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The map label for this gene is amiC [H]

Identifier: 29143251

GI number: 29143251

Start: 2982788

End: 2984041

Strand: Reverse

Name: amiC [H]

Synonym: t2899

Alternate gene names: 29143251

Gene position: 2984041-2982788 (Counterclockwise)

Preceding gene: 29143253

Following gene: 29143250

Centisome position: 62.27

GC content: 53.35

Gene sequence:

>1254_bases
ATGTCGGGAGCCAATTCAGCAATCAGCCGTCGCCGTTTACTACAAGGCGCGGGCGCCATGTGGCTATTAAGCGTGAGCCA
GGTCGGGCTGGCTGCCGTAAGCCAGGTCGTTGCGGTGCGTATCTGGCCTGCGTCCAGCTATACCCGCGTCACGGTAGAGT
CGAATCGTCTGCTGAAATATAAACAATTTGCCTTAAGTAACCCGGACCGCGTGGTGGTAGATATTGAGGATGTGAATCTG
AACTCGGTGCTTAAAGGCATTGGCGCGCAGATTCGCAGCGACGATCCGTATATCAAATCCGCAAGGGTAGGGCAGTTTGA
TCCTAAGACTGTGCGTATGGTCTTTGAGCTAAAACAAAACGTGAAGCCGCAGCTATTTGCGCTGGCGCCTGTGGCAGGCT
TTAAAGAACGTCTGGTGATAGATCTTTATCCTGCTAATGCGCAGGATATGCAGGATCCGCTGCTGGCGCTGCTGGAAGAC
TACAATAAAGGCGATCTGGATAAACAGGTGCCGCCTTCGCAAAGCGGGCCGCAGCCCGGTAAAGCGGGGCGCGATCGTCC
CATTGTCATTATGCTTGATCCGGGACACGGCGGCGAAGACCCTGGCGCGATAGGCAAATACAAAACGCGTGAAAAAGACG
TGGTGCTCCAGATAGCCCGCCGCCTGCGCGCGTTGATCGAAAAAGAAGGCAACATGAAGGTCTATATGACGCGCAATGAA
GACATCTTCATTCCGTTAAAAGTCCGGGTGGCGAAAGCGCAGAAACAGCGCGCCGACCTGTTTGTCTCTATTCATGCGGA
TGCCTTCACCAGCCGGCAGCCTAGCGGTTCATCCGTATTCGCGCTATCGACTAAAGGCGCCACCAGTACTGCGGCGAAAT
ATCTGGCGCAAACGCAGAACGCCTCGGACTTAATTGGCGGCGTGAGCAAAAGCGGTGACCGTTATGTCGACCATACGATG
TTCGATATGGTGCAATCGCTGACCATTGCCGATAGTTTGAAATTCGGTAAAGCGGTGTTGAAGCAGCTTGGTAAAATTAA
TGACCTGCATAAAAACAAGGTCGAGCAGGCGGGGTTTGCGGTGTTAAAGGCGCCCGATATTCCGTCTATTCTGGTCGAGA
CGGCGTTTATCAGTAACATTGAAGAAGAACGCAAGCTCAAGACCGCGACGTTCCAGCAACAGGTCGCAGAGTCGATTCTG
GCGGGCATTAAAGCCTATTTTGCCGATGGGGCGACGCTGGCGAGAAGAAGTTAA

Upstream 100 bases:

>100_bases
ACCCTTTTATAGATGACAGATTATGCGTCATTCGCTAAAGTTTCCGGTCAATTTGGATGAACTTTGTATGATCTCTATTT
AGTTTTTGCTCGGGAGAAGC

Downstream 100 bases:

>100_bases
CCTGAGATGACGGCGAGTTTATTGCGCGGGTAAGTAGGATGCTGCCCGCACTGTCGTTGCGGACCAGATGACAGATACAA
AAAAACACCCGTAAGGGTGT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKYKQFALSNPDRVVVDIEDVNL
NSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQNVKPQLFALAPVAGFKERLVIDLYPANAQDMQDPLLALLED
YNKGDLDKQVPPSQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTM
FDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFAVLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESIL
AGIKAYFADGATLARRS

Sequences:

>Translated_417_residues
MSGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKYKQFALSNPDRVVVDIEDVNL
NSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQNVKPQLFALAPVAGFKERLVIDLYPANAQDMQDPLLALLED
YNKGDLDKQVPPSQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTM
FDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFAVLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESIL
AGIKAYFADGATLARRS
>Mature_416_residues
SGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKYKQFALSNPDRVVVDIEDVNLN
SVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQNVKPQLFALAPVAGFKERLVIDLYPANAQDMQDPLLALLEDY
NKGDLDKQVPPSQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNED
IFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMF
DMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFAVLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESILA
GIKAYFADGATLARRS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=416, Percent_Identity=94.2307692307692, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=47.2340425531915, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=42.2222222222222, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45596; Mature: 45465

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKY
CCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCHH
KQFALSNPDRVVVDIEDVNLNSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQN
HHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
VKPQLFALAPVAGFKERLVIDLYPANAQDMQDPLLALLEDYNKGDLDKQVPPSQSGPQPG
CCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
CEEEEEEEEEHHHHHCCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCEE
VLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESILAGIKAYFADGATLARRS
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
SGANSAISRRRLLQGAGAMWLLSVSQVGLAAVSQVVAVRIWPASSYTRVTVESNRLLKY
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCHH
KQFALSNPDRVVVDIEDVNLNSVLKGIGAQIRSDDPYIKSARVGQFDPKTVRMVFELKQN
HHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHC
VKPQLFALAPVAGFKERLVIDLYPANAQDMQDPLLALLEDYNKGDLDKQVPPSQSGPQPG
CCCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAGRDRPIVIMLDPGHGGEDPGAIGKYKTREKDVVLQIARRLRALIEKEGNMKVYMTRNE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DIFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQN
CEEEEEEEEEHHHHHCCCCEEEEEECCHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCC
ASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLKQLGKINDLHKNKVEQAGFA
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCEE
VLKAPDIPSILVETAFISNIEEERKLKTATFQQQVAESILAGIKAYFADGATLARRS
EEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]