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The map label for this gene is yqcB

Identifier: 29143227

GI number: 29143227

Start: 2956978

End: 2957760

Strand: Reverse

Name: yqcB

Synonym: t2873

Alternate gene names: 29143227

Gene position: 2957760-2956978 (Counterclockwise)

Preceding gene: 29143228

Following gene: 29143226

Centisome position: 61.72

GC content: 58.24

Gene sequence:

>783_bases
ATGCTGGAGATCCTCTATCAAGACCCGTGGCTGGTTGCCGTGAATAAACCCGCAGGCTGGCTTGTTCACCGGAGCTGGCT
GGATCGCGACGAAAAAGTCGTGGTCATGCAAACGGTGCGCGACCAAATCGGCCAGCATGTTTTTACCGCCCACCGTCTCG
ACAGGCCCACGTCGGGCGTACTGTTAATGGGGCTGTCCAGCGAAGCGGGACGCCGTCTGGCGCAGCAGTTCGAGCAGCAC
CACATCCGTAAACGCTACCATGCCATTGTGCGCGGCTGGCTGATAGATGACGCGGTACTGGATTATCCGCTGGTGGAAGA
GCGCGATAAAATTGCCGATAAATTCGCCCGCGAAGATAAAGCGCCCCAGCCGGCTGTAACGCAGTATCGCGGGCTGGCGA
CGGTCGAAATGGCGGTGCCGACCGGGCGTTATCCCACCACGCGTTATGGCCTGGTTGAGCTGGAACCAAAAACGGGACGC
AAGCACCAGCTTCGTCGTCATCTGGCGCATCTGCGTCATCCTATCATCGGCGACAGCAAGCATGGCGATTTGCGACAAAA
CCGCAGCGCCGCAGAGCATTTTGCCTGTCGTCGCCTGATGCTTCATGCCAGTCGGCTTGAACTGACGCACCCCTTCACCG
GACAGCCGTTGATAATTCAGGCCGGGTTGGATGAAACCTGGATGCAGGTGCTGACACAGTTTGGCTGGCGTGGACTTCTC
CCTGACAATGAAAGGGTTGAGTTTACGGCGGCGTCCCGGCAGGATGAGACTCATCTGACATAA

Upstream 100 bases:

>100_bases
CTTATTATGAAATGGCGCTGGCGGCGGATCATCCGCAGCGGGAGGCGATCCTGGCGGTTTTGCAGGATCTGGATGCGCTA
TTCGTCCGCGATAAATCCTG

Downstream 100 bases:

>100_bases
TTCAGGGAGATACGCATAATGGCGGAAATTGGTATTTTTGTCGGGACCATGTATGGCAACTCACTGCTGGTGGCGGAAGA
AGCGGAAGCGATCCTCGCCA

Product: tRNA pseudouridine synthase C

Products: NA

Alternate protein names: tRNA pseudouridylate synthase C; tRNA-uridine isomerase C

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLEILYQDPWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRRLAQQFEQH
HIRKRYHAIVRGWLIDDAVLDYPLVEERDKIADKFAREDKAPQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLIIQAGLDETWMQVLTQFGWRGLL
PDNERVEFTAASRQDETHLT

Sequences:

>Translated_260_residues
MLEILYQDPWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRRLAQQFEQH
HIRKRYHAIVRGWLIDDAVLDYPLVEERDKIADKFAREDKAPQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLIIQAGLDETWMQVLTQFGWRGLL
PDNERVEFTAASRQDETHLT
>Mature_260_residues
MLEILYQDPWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGVLLMGLSSEAGRRLAQQFEQH
HIRKRYHAIVRGWLIDDAVLDYPLVEERDKIADKFAREDKAPQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGR
KHQLRRHLAHLRHPIIGDSKHGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLIIQAGLDETWMQVLTQFGWRGLL
PDNERVEFTAASRQDETHLT

Specific function: Responsible for synthesis of pseudouridine from uracil- 65 in transfer RNAs

COG id: COG0564

COG function: function code J; Pseudouridylate synthases, 23S RNA-specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rluA family

Homologues:

Organism=Homo sapiens, GI221316667, Length=186, Percent_Identity=32.7956989247312, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI221316663, Length=204, Percent_Identity=29.9019607843137, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1789155, Length=255, Percent_Identity=88.6274509803922, Blast_Score=469, Evalue=1e-133,
Organism=Escherichia coli, GI1787327, Length=233, Percent_Identity=31.3304721030043, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786244, Length=225, Percent_Identity=34.6666666666667, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788946, Length=228, Percent_Identity=27.6315789473684, Blast_Score=81, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6320168, Length=180, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324506, Length=187, Percent_Identity=25.668449197861, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583449, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583447, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583445, Length=186, Percent_Identity=31.1827956989247, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921090, Length=187, Percent_Identity=32.0855614973262, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUC_SALTI (Q8Z439)

Other databases:

- EMBL:   AL627276
- EMBL:   AE014613
- RefSeq:   NP_457360.1
- RefSeq:   NP_806569.1
- ProteinModelPortal:   Q8Z439
- SMR:   Q8Z439
- GeneID:   1068708
- GeneID:   1249400
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t2873
- KEGG:   sty:STY3103
- HOGENOM:   HBG750656
- OMA:   MPVAIGR
- ProtClustDB:   PRK11112
- BioCyc:   SENT209261:T2873-MONOMER
- BioCyc:   SENT220341:STY3103-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006224
- InterPro:   IPR006145

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 30056; Mature: 30056

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS01129 PSI_RLU

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEILYQDPWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGV
CCEEEECCCEEEEECCCCCCEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMGLSSEAGRRLAQQFEQHHIRKRYHAIVRGWLIDDAVLDYPLVEERDKIADKFAREDK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
APQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGRKHQLRRHLAHLRHPIIGDSK
CCCCHHHHHCCCEEEEEECCCCCCCCCEECEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLIIQAGLDETWMQVLTQFGWRGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
PDNERVEFTAASRQDETHLT
CCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MLEILYQDPWLVAVNKPAGWLVHRSWLDRDEKVVVMQTVRDQIGQHVFTAHRLDRPTSGV
CCEEEECCCEEEEECCCCCCEEEHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCE
LLMGLSSEAGRRLAQQFEQHHIRKRYHAIVRGWLIDDAVLDYPLVEERDKIADKFAREDK
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
APQPAVTQYRGLATVEMAVPTGRYPTTRYGLVELEPKTGRKHQLRRHLAHLRHPIIGDSK
CCCCHHHHHCCCEEEEEECCCCCCCCCEECEEEECCCCCHHHHHHHHHHHHHCCCCCCCC
HGDLRQNRSAAEHFACRRLMLHASRLELTHPFTGQPLIIQAGLDETWMQVLTQFGWRGLL
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCHHHHHHHHHHCCCCCC
PDNERVEFTAASRQDETHLT
CCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504