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The map label for this gene is rumA [H]

Identifier: 29143220

GI number: 29143220

Start: 2946600

End: 2947895

Strand: Reverse

Name: rumA [H]

Synonym: t2866

Alternate gene names: 29143220

Gene position: 2947895-2946600 (Counterclockwise)

Preceding gene: 29143222

Following gene: 29143219

Centisome position: 61.52

GC content: 54.24

Gene sequence:

>1296_bases
ATGGCGCAATTCTACTCTGCAAAACGGCGCGTGACGACGCGTCAGATCATAACCGTTAAAGTCAATGACCTCGATTCATT
TGGTCAGGGCGTAGCCCGTCATAACGGAAAGGCGCTGTTTATCCCCGGTTTATTGCCTGAGGAAAGCGCAGAAGTCATTA
TTACAGAAGATAAAAAGCAATTTGCCCGCGCACGCGTTTCGCGCCGTTTGAATGATAGCCCGGAACGAGAAACGCCGCGC
TGCCCGCATTTTGGCGTCTGCGGCGGTTGTCAGCAACAGCATGTCGGCATCGACCTGCAACAACGCAGCAAAAGCGCGGC
GCTGGCGCGTTTAATGAAACATGAGGTGAATGACATCATCGCCGGCGCGTCCTGGGGTTATCGGCGTCGTGCGCGGTTAA
GTCTGAATTGCCCGCCGGATAAACCGCTGCAAATGGGATTTCGCAAAGCGGGTTCCAGCGATATCGTCAATGTCGAACAG
TGCCCCGTTTTGGCGCCCCAGCTTGAAGCATTGCTGCCCCGCATCAGGGCGTGTCTGGCGAGTTTACACGGCACCCGACA
TCTTGGGCATGTTGAGCTGGTACAGGCGGGAAGCGGTACGCTGATGATTCTGCGCCATACCGCGCCATTAAGTGCGGCGG
ATAAAGAAAAACTGGAACGCTTTTCGCATTCTGAGGGATTGTCTCTGTTCCTGGCGCCGTTTAGCGAGATACTGGAAACG
GTATCTGGCGAAGCGCCCTGGTATGATTCGCACGGCTTACGTTTAACCTTCAGTCCGCGAGACTTTATTCAGGTTAATGA
GGCGGTAAACCAGCAAATGGTCGCCCGCGCGCTGGAGTGGCTTGATGTGCGCGCCGAGGATCGTGTCCTGGATCTGTTCT
GCGGAATGGGGAATTTTACGCTACCGCTGGCAACGCGCGCGGCAAGCGTGGTAGGGGTTGAGGGCGTTCAGGCGCTGGTA
GAAAAAGGCCGTGAAAACGCCATCCGCAATGGTTTACATAATGTGACATTCTTCCACGAGAACCTGGAGGAAGATGTCAC
GAAGCAGCCGTGGGCGAAAAACGGCTTTGACAAAGTCTTACTCGATCCTGCGCGTGCGGGGGCTACAGGAGTGATGCGAC
ATATTATAAAATTAAAACCTATTCGCATTGTTTATGTATCCTGTAACCCGGCGACGCTGGCGCGCGATAGTGAAGCGCTG
GTCAATGCGGGATATGAGGTTACGCGTTTAGCGATGCTCGACATGTTCCCGCACACAGGACATCTGGAATCAATGGTTCT
GTTCGAGCGCATGTAA

Upstream 100 bases:

>100_bases
GGATCAGAATCATCATGCGTGCGCGCAGGCTGTAGTTGGTCATGGAGTTCCGTTATGGGACAATTAGGGTCACACTGTTA
AATTGAGAAAAAGCTGGTCG

Downstream 100 bases:

>100_bases
TGATTACCGGCTTACCGACTTCGGTAGGCCTGGTCCCTTAAGGAGAGGACGATGGTCGCGGTAAGAAGTGCACATATTAA
TAAAGCTGGTGAATTTGATC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQFARARVSRRLNDSPERETPR
CPHFGVCGGCQQQHVGIDLQQRSKSAALARLMKHEVNDIIAGASWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQ
CPVLAPQLEALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
VSGEAPWYDSHGLRLTFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVVGVEGVQALV
EKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEAL
VNAGYEVTRLAMLDMFPHTGHLESMVLFERM

Sequences:

>Translated_431_residues
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQFARARVSRRLNDSPERETPR
CPHFGVCGGCQQQHVGIDLQQRSKSAALARLMKHEVNDIIAGASWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQ
CPVLAPQLEALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
VSGEAPWYDSHGLRLTFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVVGVEGVQALV
EKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEAL
VNAGYEVTRLAMLDMFPHTGHLESMVLFERM
>Mature_430_residues
AQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQFARARVSRRLNDSPERETPRC
PHFGVCGGCQQQHVGIDLQQRSKSAALARLMKHEVNDIIAGASWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQC
PVLAPQLEALLPRIRACLASLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILETV
SGEAPWYDSHGLRLTFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFTLPLATRAASVVGVEGVQALVE
KGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVLLDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALV
NAGYEVTRLAMLDMFPHTGHLESMVLFERM

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=207, Percent_Identity=33.8164251207729, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI51173878, Length=207, Percent_Identity=33.8164251207729, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI269784680, Length=193, Percent_Identity=28.4974093264249, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI269784676, Length=193, Percent_Identity=28.4974093264249, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI20149688, Length=193, Percent_Identity=28.4974093264249, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI269784678, Length=193, Percent_Identity=28.4974093264249, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI1789148, Length=432, Percent_Identity=84.2592592592593, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI1787083, Length=373, Percent_Identity=26.8096514745308, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=34.0659340659341, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24666579, Length=215, Percent_Identity=29.7674418604651, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 47878; Mature: 47747

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQ
CCCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHH
FARARVSRRLNDSPERETPRCPHFGVCGGCQQQHVGIDLQQRSKSAALARLMKHEVNDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AGASWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQCPVLAPQLEALLPRIRACLA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHH
SLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
VSGEAPWYDSHGLRLTFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFT
HCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
LPLATRAASVVGVEGVQALVEKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
LDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALVNAGYEVTRLAMLDMFPHTG
ECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCCC
HLESMVLFERM
CHHHHHHHHCC
>Mature Secondary Structure 
AQFYSAKRRVTTRQIITVKVNDLDSFGQGVARHNGKALFIPGLLPEESAEVIITEDKKQ
CCHHHHHHCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCHHH
FARARVSRRLNDSPERETPRCPHFGVCGGCQQQHVGIDLQQRSKSAALARLMKHEVNDII
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AGASWGYRRRARLSLNCPPDKPLQMGFRKAGSSDIVNVEQCPVLAPQLEALLPRIRACLA
CCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEHHHCCCCHHHHHHHHHHHHHHHH
SLHGTRHLGHVELVQAGSGTLMILRHTAPLSAADKEKLERFSHSEGLSLFLAPFSEILET
HHHCCCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCEEEHHHHHHHHHH
VSGEAPWYDSHGLRLTFSPRDFIQVNEAVNQQMVARALEWLDVRAEDRVLDLFCGMGNFT
HCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
LPLATRAASVVGVEGVQALVEKGRENAIRNGLHNVTFFHENLEEDVTKQPWAKNGFDKVL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
LDPARAGATGVMRHIIKLKPIRIVYVSCNPATLARDSEALVNAGYEVTRLAMLDMFPHTG
ECCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHCCCCHHHHHCCCHHHHHHHHHHCCCCC
HLESMVLFERM
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA