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The map label for this gene is hycD [H]

Identifier: 29143111

GI number: 29143111

Start: 2834368

End: 2835291

Strand: Reverse

Name: hycD [H]

Synonym: t2752

Alternate gene names: 29143111

Gene position: 2835291-2834368 (Counterclockwise)

Preceding gene: 29143112

Following gene: 29143110

Centisome position: 59.17

GC content: 58.23

Gene sequence:

>924_bases
ATGAGTGTGTTTTATCCGTTAATTCAGGCGCTGGTGCTATTTGCCGTTGCGCCATTGCTGTCCGGTATTACTCGCGTGGC
GCGCGCCCGTCTACACAATCGCCGTGGGCCGGGCGTGTTGCAGGAGTACCGCGACATTATCAAACTGCTCGGTCGTCAGA
GTATTGCGCCCGCTGATTCCGGCTGGGTTTTTCGCCTGACGCCGTTTGTGATGGTCGGCGTGATGCTGACTATCGCCACC
GCGCTGCCGGTCGTCACCGTTGGATCTCCGTTGCCGCAACTGGGTGATTTAATCACCCTGATTTATCTGTTCGCGATTGC
GCGCTTCTTCTTCTCTATTGCAGGCCTGGATACCGGCAGTCCGTTCACTGCGATCGGCGCCAGCCGTGAAGCGATGCTCG
GCGTGCTGGTGGAGCCGATTCTGCTGCTGGGTCTGTGGGTAGCCGCGCAGGTGGCGGGTTCAACTCATATCAGCAATATC
GCCGATACCATTTATCACTGGCCTGTCGCGCGCAGTATTCCGCTGATTCTGGCGCTGTGCGCCTGCGCCTTTGCCACCTT
TATCGAAATGGGCAAGCTGCCGTTCGACCTGGCGGAAGCGGAGCAGGAGTTGCAGGAAGGGCCGTTGACCGAATACAGCG
GTAGCGGCTTTGCGGTGCTGAAATGGGGCATCAGCCTGAAACAACTGGTGGTGCTGCAAATGTTCGTCGGCGTGTTTTTG
CCGTGGGGACAGATGGAAACCTTCAGCGCAGGCGGACTGTTACTGGCGCTGGTGATTGCCGTCGTCAAGCTGATTGTCGG
CGTACTGGTGATCGCCCTGTTTGAAAACAGCATGGCGCGACTGCGTTTTTGCGCCACTTCACGCGTGACCTGGGCCGGTT
TTGGGTTTGCGTTTTTAGCCTTCGTCTCCTTGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGCAGAACGCGGCGGCCGCGGGGCTGTTCCGTCGTCTGGCGCTGATTGAGCTGGCGGTGCTGGTGGTGATTGTG
GTTTCACGAGGAGCCTGAAA

Downstream 100 bases:

>100_bases
TTTAAGAGAGTTTGAGCATGTTTGAAGAAAAATTAGGTCAACAATACCTTGCGGCGCTGCACCAGGCGTTTCCGGGCGTC
GTGATGGACGAAGCCTGGCA

Product: formate hydrogenlyase subunit 4

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSVFYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSIAPADSGWVFRLTPFVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLIYLFAIARFFFSIAGLDTGSPFTAIGASREAMLGVLVEPILLLGLWVAAQVAGSTHISNI
ADTIYHWPVARSIPLILALCACAFATFIEMGKLPFDLAEAEQELQEGPLTEYSGSGFAVLKWGISLKQLVVLQMFVGVFL
PWGQMETFSAGGLLLALVIAVVKLIVGVLVIALFENSMARLRFCATSRVTWAGFGFAFLAFVSLLAA

Sequences:

>Translated_307_residues
MSVFYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSIAPADSGWVFRLTPFVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLIYLFAIARFFFSIAGLDTGSPFTAIGASREAMLGVLVEPILLLGLWVAAQVAGSTHISNI
ADTIYHWPVARSIPLILALCACAFATFIEMGKLPFDLAEAEQELQEGPLTEYSGSGFAVLKWGISLKQLVVLQMFVGVFL
PWGQMETFSAGGLLLALVIAVVKLIVGVLVIALFENSMARLRFCATSRVTWAGFGFAFLAFVSLLAA
>Mature_306_residues
SVFYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSIAPADSGWVFRLTPFVMVGVMLTIATA
LPVVTVGSPLPQLGDLITLIYLFAIARFFFSIAGLDTGSPFTAIGASREAMLGVLVEPILLLGLWVAAQVAGSTHISNIA
DTIYHWPVARSIPLILALCACAFATFIEMGKLPFDLAEAEQELQEGPLTEYSGSGFAVLKWGISLKQLVVLQMFVGVFLP
WGQMETFSAGGLLLALVIAVVKLIVGVLVIALFENSMARLRFCATSRVTWAGFGFAFLAFVSLLAA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=90.5537459283388, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI87082115, Length=302, Percent_Identity=53.6423841059603, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1788618, Length=201, Percent_Identity=29.8507462686567, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 33029; Mature: 32898

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00667 COMPLEX1_ND1_1 ; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSIAPADS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPFVMVGVMLTIATALPVVTVGSPLPQLGDLITLIYLFAIARFFFSIAGLDTGS
CCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPILLLGLWVAAQVAGSTHISNIADTIYHWPVARSIPLILALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLTEYSGSGFAVLKWGISLKQLVVLQMFVGVFL
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
PWGQMETFSAGGLLLALVIAVVKLIVGVLVIALFENSMARLRFCATSRVTWAGFGFAFLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FVSLLAA
HHHHHCC
>Mature Secondary Structure 
SVFYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSIAPADS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPFVMVGVMLTIATALPVVTVGSPLPQLGDLITLIYLFAIARFFFSIAGLDTGS
CCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPILLLGLWVAAQVAGSTHISNIADTIYHWPVARSIPLILALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLTEYSGSGFAVLKWGISLKQLVVLQMFVGVFL
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
PWGQMETFSAGGLLLALVIAVVKLIVGVLVIALFENSMARLRFCATSRVTWAGFGFAFLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
FVSLLAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]