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The map label for this gene is hycE [H]

Identifier: 29143110

GI number: 29143110

Start: 2832641

End: 2834350

Strand: Reverse

Name: hycE [H]

Synonym: t2751

Alternate gene names: 29143110

Gene position: 2834350-2832641 (Counterclockwise)

Preceding gene: 29143111

Following gene: 29143109

Centisome position: 59.15

GC content: 57.43

Gene sequence:

>1710_bases
ATGTTTGAAGAAAAATTAGGTCAACAATACCTTGCGGCGCTGCACCAGGCGTTTCCGGGCGTCGTGATGGACGAAGCCTG
GCAGACCAAAGATCAGCTGACTATTACGGTAAAAGTGAACTATCTGCCGGAAGTGGTGGAGTTTCTTTACTACCAGCAGG
GTGGGTGGCTGTCGGTGCTGTTCGGTAATGACGAACGCCAGTTGTGCGGCCACTATGCCGTTTATTACGTGCTGTCGATG
GAGCAGGGCACCAAGTGCTGGATTACCGTCCGTGTTGAAGTGGATGCCAATAAGCTGGAATTCCCATCCGTTACGCCGCG
CGTGCCGGCTGCCGTGTGGGGTGAGCGCGAAGTACGCGACATGTACGGTTTAATCCCGGTCGGTCTGCCGGACGAGCGCC
GTCTGGTGCTGCCGGACGACTGGCCGGATGAACTCTATCCGCTGCGTAAAGACAGCATGGATTATCGTCAGCGCCCGGCG
CCGACCACCGATGCGGAAACCTACGAGTTCATTAACGAGCTGGGTGACAAGAAAAATAACGTGGTGCCGATTGGCCCGCT
GCATGTCACTTCCGATGAACCGGGCCACTTCCGTCTGTTCGTCGATGGCGAAAACATTATCGATGCCGACTACCGCCTGT
TCTACGTCCACCGTGGTATGGAAAAACTGGCGGAAACCCGCATGGGTTATAACGAAGTCACCTTCCTGTCGGATCGCGTG
TGTGGAATCTGCGGTTTCGCGCACAGCACTGCCTACACCACTTCCGTGGAAAACGCGATGGGCATTCAGGTGCCGGAGCG
TGCGCAGATGATCCGCGCCATTCTGCTGGAAGTGGAGCGTCTGCACTCGCACCTGCTGAACCTAGGCCTCGCCTGCCACT
TTACCGGCTTTGACTCCGGCTTTATGCAGTTCTTCCGCGTGCGTGAAACCTCCATGAAGATGGCGGAAATACTGACCGGG
GCGCGCAAAACTTACGGTCTGAACCTGATCGGCGGGATTCGCCGCGATCTGCTCAAAGAGGACATGATCCAGACCCGTCA
ACTGGCGCAGCAGATGCGTCGTGACGTGCAGGAGCTGGTGGACATGCTGCTGAGCACGCCGAATATGGAACAGCGTACCG
TGGGTATCGGCCGTCTGGACCCGGAAATTGCCCGCGACTTCAGTAATGTCGGCCCGATGGTGCGCGCCAGCGGTCACGCC
CGCGACACCCGCGCCGACCACCCGTTTGTCGGCTACGGTCTGCTGCCGATGGAAGTACATAGCGAGCAGGGCTGCGACGT
GATTTCTCGTCTGAAAGTCCGTATCAACGAAGTCTACACCTCGCTGAATATGATCGATTTCGGTCTGGATAATCTACCGG
GCGGCCCGCTGATGGTGGAAGGCTTTACCTATATTCCGCACCGTTTTGCGCTCGGCTTCGCTGAAGCGCCGCGTGGTGAC
GATATCCACTGGAGCATGACCGGCGACAACCAGAAGCTTTACCGCTGGCGCTGCCGTGCGGCAACCTACGCCAACTGGCC
GACCCTGCGTTATATGCTGCGTGGCAACACCGTCTCCGACGCGCCGCTGATCATCGGTAGCCTCGATCCTTGCTACTCCT
GTACCGACCGGATGACCGTGGTCGATGTACGTAAGAAGAAGAGCAAAGTCGTGCCGTACAAAGAACTTGAGCGCTACAGC
ATTGAGCGTAAAAACTCGCCGCTGAAATAA

Upstream 100 bases:

>100_bases
TGCGTTTTTGCGCCACTTCACGCGTGACCTGGGCCGGTTTTGGGTTTGCGTTTTTAGCCTTCGTCTCCTTGCTGGCGGCG
TGATTTAAGAGAGTTTGAGC

Downstream 100 bases:

>100_bases
GGAATCGCCATGTTTACCTTTATCAAAAAAGTTATCAAAACCGGCACGGCGACCTCGTCTTATCCGCTGGAGCCGATTGC
GGTTGATAAAAACTTCCGCG

Product: formate hydrogenlyase subunit 5

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MFEEKLGQQYLAALHQAFPGVVMDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVLFGNDERQLCGHYAVYYVLSM
EQGTKCWITVRVEVDANKLEFPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELVDMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTSLNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK

Sequences:

>Translated_569_residues
MFEEKLGQQYLAALHQAFPGVVMDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVLFGNDERQLCGHYAVYYVLSM
EQGTKCWITVRVEVDANKLEFPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELVDMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTSLNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK
>Mature_569_residues
MFEEKLGQQYLAALHQAFPGVVMDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVLFGNDERQLCGHYAVYYVLSM
EQGTKCWITVRVEVDANKLEFPSVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELVDMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTSLNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=387, Percent_Identity=25.8397932816537, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI260898743, Length=379, Percent_Identity=26.3852242744063, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1789076, Length=569, Percent_Identity=96.8365553602812, Blast_Score=1152, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=563, Percent_Identity=72.113676731794, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=512, Percent_Identity=25.78125, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17568379, Length=387, Percent_Identity=25.8397932816537, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17555284, Length=400, Percent_Identity=25.5, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI221459469, Length=387, Percent_Identity=25.5813953488372, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24638644, Length=387, Percent_Identity=26.6149870801034, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 65099; Mature: 65099

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEEKLGQQYLAALHQAFPGVVMDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVL
CCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEE
FGNDERQLCGHYAVYYVLSMEQGTKCWITVRVEVDANKLEFPSVTPRVPAAVWGEREVRD
ECCCHHHHHHHEEEEEEEEECCCCEEEEEEEEEECCCCEECCCCCCCCCHHHCCCHHHHH
MYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGDKKNN
HHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCC
VVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
EEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHH
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSG
HHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
FMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELV
HHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVH
HHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECCEEEEEC
SEQGCDVISRLKVRINEVYTSLNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEECCHHHHCCCCCCCCCC
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTV
CEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHCCCCCEEE
VDVRKKKSKVVPYKELERYSIERKNSPLK
EEEHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFEEKLGQQYLAALHQAFPGVVMDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVL
CCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEE
FGNDERQLCGHYAVYYVLSMEQGTKCWITVRVEVDANKLEFPSVTPRVPAAVWGEREVRD
ECCCHHHHHHHEEEEEEEEECCCCEEEEEEEEEECCCCEECCCCCCCCCHHHCCCHHHHH
MYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGDKKNN
HHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCC
VVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
EEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHH
CGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSG
HHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
FMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELV
HHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVH
HHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECCEEEEEC
SEQGCDVISRLKVRINEVYTSLNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFAEAPRGD
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCEEECCHHHHCCCCCCCCCC
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTV
CEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEECCCCHHCCCCCEEE
VDVRKKKSKVVPYKELERYSIERKNSPLK
EEEHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]