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The map label for this gene is pinE [H]

Identifier: 29143036

GI number: 29143036

Start: 2754056

End: 2754628

Strand: Reverse

Name: pinE [H]

Synonym: t2667

Alternate gene names: 29143036

Gene position: 2754628-2754056 (Counterclockwise)

Preceding gene: 229220624

Following gene: 29143035

Centisome position: 57.48

GC content: 50.96

Gene sequence:

>573_bases
ATGATCATGCTTATTGGCTATGTACGCGTGTCAACAAATGACCAGAATACCGATTTGCAACGCAATGCACTGAACTGCGC
GGGATGTGAGCGGATTTTTGAGGACAAAATCAGTGGCACCAAGTCCGACAGACCGGGGCTGAAAAAGCTGCTCAGGACAC
TATCGGCAGGAGACACGCTGGTTGTCTGGAAGCTGGACAGACTGGGGCGCAGTATGCGGCATCTTGTTACGCTGATAGAA
GAGCTGCGCCAGCGTGGCGTGAATTTCCGAAGCCTGACTGACAGTATTGATACCAGCACCCCAATGGGCCGTTTCTTTTT
TCATGTCATGGGTGCCCTGGCTGAAATGGAACGCGAACTGATAGTTGAACGTACCAGGGCAGGGCTGGCTGCAGCTCGTG
CTAAAGGCAGAGTAGGTGGACGCCGTCCTAAGTTGACCAGCGAACAGTGGGCACAGATTGGGCGTTTACTCGAGGCCGGA
GAATCAAGACAGCGTATTGCACTGATTTTTGATGTAGGCGTTTCTACCATTTATCGAAAATTTCCGGCAAATAAGAACAA
TAAATCTCCCTGA

Upstream 100 bases:

>100_bases
GCGCACCAGCCACACGATTTATTGGTTCTTGAAAACCAACGTTTATGAAAATGCAGAGGTAACAGCTAACTGGCATCATC
TCCGGTTTTTATTCAGGGGA

Downstream 100 bases:

>100_bases
ATCAGCATTATTTTGATTATTCCTGCAAGCGGACAAATACCGTCATTTTGTGTGAATAACGGTACAACTGCGCTTAGCTG
TTTGTCGGACACAATCACTT

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MIMLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIE
ELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAG
ESRQRIALIFDVGVSTIYRKFPANKNNKSP

Sequences:

>Translated_190_residues
MIMLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIE
ELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAG
ESRQRIALIFDVGVSTIYRKFPANKNNKSP
>Mature_190_residues
MIMLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIE
ELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAG
ESRQRIALIFDVGVSTIYRKFPANKNNKSP

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=86.7403314917127, Blast_Score=334, Evalue=2e-93,
Organism=Escherichia coli, GI1787827, Length=186, Percent_Identity=37.6344086021505, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=37.6344086021505, Blast_Score=114, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21390; Mature: 21390

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTL
CEEEEEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
VVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMEREL
EEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGESRQRIALIFDVGVSTIYRK
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
FPANKNNKSP
CCCCCCCCCC
>Mature Secondary Structure
MIMLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPGLKKLLRTLSAGDTL
CEEEEEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEE
VVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGRFFFHVMGALAEMEREL
EEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
IVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEAGESRQRIALIFDVGVSTIYRK
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
FPANKNNKSP
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]