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The map label for this gene is clpB [H]

Identifier: 29142966

GI number: 29142966

Start: 2668402

End: 2671065

Strand: Reverse

Name: clpB [H]

Synonym: t2591

Alternate gene names: 29142966

Gene position: 2671065-2668402 (Counterclockwise)

Preceding gene: 29142975

Following gene: 29142965

Centisome position: 55.74

GC content: 59.05

Gene sequence:

>2664_bases
ATGGAAACTCCTGTTTCACGCAGTGCGTTATATGGAAAACTGGCCGGCCCACTATTCCGGTCGCTGGAATCGGCAACGGC
ATTTTGCAAACTACGCTCTAATCCCTGGGGTGAGCTGACTCACTGGCTGCACCAGTTAACACAGCAGCCCGATAACGATA
TTCTCCACGTTCTTCGGCATTACCAGATCCCTCTTTCTGATGTGGAGAAAGCGTTACTCCGGCAACTGGATATGCTGCCC
GCCGGGGCCAGCGCCATTAGTGATTTTTCTCACCATATCGATCTCAGCGTTGAAAAGGCCTGGATGCTGGCGAGCGTCCG
TTACGGCGATAACAAAATTCGCAGCGGCTGGTTGCTGCTGGCCTTGTTGACCACGCCAGAACTGCGTCGGGTACTGAGCA
GTATCTGCGCGCCGCTGGCCACGCTTCCGGTTGATGAACTGACGGAAATACTGCCCTCGTTGATCGAAACATCGCCGGAA
GCGCAGGAGCGCCCTTACGACGGCTCCGGTCTGGCATCAGCCATTCCCGGTGAAAGCAGCCAGGCGATTCCCAACGGCGT
GCAGGACGGTAAATCCGCGCTGGCAAAATACTGTCAGGACATGACGGCACAGGCGCGCGACGGCAAAATCGACCCGGTGA
CGGGGCGTGAGCATGAAATCCGCACCATGACGGATATTCTGCTGCGCCGTCGCCAGAATAATCCCCTACTGACTGGTGAG
GCGGGCGTCGGAAAGACGGCGGTCGTCGAAGGTTTTGCCCTCGCGATTGCGCAGGGGGAAGTGCCGCCCGCGCTGCGGGA
AGTACGGCTGCTGGCGCTGGACGTTGGCGCTCTGTTGGCCGGAGCCAGCATGAAAGGCGAGTTTGAATCGCGTCTGAAAG
GGTTACTGGAAGAGGCCGGGCGCTCGCCGCAGCCGGTTATTCTGTTTGTCGATGAAGTTCACACTCTGGTGGGCGCGGGC
GGCGCATCCGGCACGGGCGATGCCGCTAACCTGCTGAAACCGGCGCTGGCGTGCGGCACCCTGCGGACTATCGGCGCCAC
CACCTGGAGCGAATACAAGCGCCATATTGAGAAAGATCCGGCGCTGACCCGTCGTTTTCAGGTGTTGCAGATTGCCGAGC
CGGAAGAGATCCCCGCAATGGAAATGGTGCGTGGTCTGGTGGATACGCTGGAAAAACACCATAACGTACTGATTCTGGAT
GAGGCGGTACGTGCGGCGGTACAGCTTTCTCACCGCTACATTCCCGCCCGGCAGTTGCCGGATAAGGCCATCAGCCTACT
GGATACCGCCGCGGTCCGCGTGGCGCTGACGCTGCACACGCCGCCTGCCAGCGTACAGTTCCTGCGTCAGCAGCTAAAAG
CGGCGGAAATGGAACGGTCGCTGTTGCAGAAGCAGGAAAAAATGGGGATTCAGTCAGATGAGCGGTGCGATGCGCTGACG
GCGCGAATTTTCTCGCTCAACGATGAACTGACTGCATCCGAATCCCGCTGGCAGCGGGAGCTGGAACTGGTACATACGTT
GCAGGAACTGCGTGTCGCAGAGTCTGATGCTGATGACAAAACCACGCTGCAACAGGCCGAAACAGCGCTAAGGGAGTGGC
AGGGCGACGCGCCGGTGGTGTTCCCGGAAGTCAGCGCGGCGATTGTCGCGGCGATTGTCGCCGACTGGACCGGTATTCCT
GCCGGGCGCATGGTGAAAGATGAGGCCAGCCAGGTGCTGGAACTGCCTGCCCGACTGGCGCAACGCGTTACCGGGCAAGA
CGGCGCGCTGGCGCAGATTGGTGAACGTATTCAGACCGCCAGGGCGGGACTGGGCGATCCACGCAAACCGGTGGGCGTGT
TTATGCTGGCCGGGCCGTCCGGTGTCGGTAAAACCGAAACCGCGCTGGCGCTGGCGGAGGCTATCTACGGCGGTGAGCAG
AACCTGGTAACCATCAATATGAGCGAGTTCCAGGAGGCTCACACCGTTTCCACACTGAAAGGCGCGCCGCCCGGCTATGT
GGGCTATGGCGAGGGTGGTGTGCTGACGGAAGCGGTGCGTCGTCACCCCTGGAGCGTAGTGCTGCTCGACGAGATCGAAA
AAGCGCACCATGACGTCCATGAACTCTTCTATCAGGTGTTTGACAAGGGCGGGATGGAGGACGGTGAGGGAACACATGTC
GATTTCAAAAACACCACGCTACTACTCACCACCAATGTAGGTTCCGACCTCATCAGCCAGATGTGTGAAGATCCGGCCTT
AATGCCCGATGCTACGGGGCTTAAAGAGGCGCTAATGCCGGAATTGCGCAAGCATTTCCCGGCGGCATTTCTGGGCCGCG
TGACGGTGATCCCTTACCTGCCGCTGGATGAAACGTCGCGTGGCGTGATTGCCCGTCTGCATCTTGACCGGCTGGTGGCG
CGGATGGGTGAACAGCACGGGGTGACGCTGACGTATAGTGAGGAACTGGTCGCACATATTGTGGCGTGCTGTCCAATGCA
TGAAACGGGCGCGCGGTTGCTGATTGGCTACATCGAACAGCACATCCTGCCACAACTGTCGCGCTACTGGTTGCAGGCCA
TGACGGAAAAAGCCGCTATCAGGCAGATTGATATCGGCGTTAATGGTGATGAGCAGATTGTTTTTGAGACAACCTCGCAG
GAGGGAATATGCCAAAAGAGTTAA

Upstream 100 bases:

>100_bases
GTTTCGATATTTTGTCGAACATTTTAATAAAGAATTGTTTATATATTCATGTGCATTGATGCATTATTTTTATGAATTTT
TATGTCACAAGGCATAACAA

Downstream 100 bases:

>100_bases
GTGGTGCGCAGTGGGTTTATGGCGTGACTAAGTTTGTCGGCGGAAATACAGATAAACCCCACTGGTCAATTGATGGGCAT
TAAATATGACATTACGTGAC

Product: ClpB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 887; Mature: 887

Protein sequence:

>887_residues
METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWGELTHWLHQLTQQPDNDILHVLRHYQIPLSDVEKALLRQLDMLP
AGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLLALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPE
AQERPYDGSGLASAIPGESSQAIPNGVQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGE
AGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAGRSPQPVILFVDEVHTLVGAG
GASGTGDAANLLKPALACGTLRTIGATTWSEYKRHIEKDPALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILD
EAVRAAVQLSHRYIPARQLPDKAISLLDTAAVRVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERCDALT
ARIFSLNDELTASESRWQRELELVHTLQELRVAESDADDKTTLQQAETALREWQGDAPVVFPEVSAAIVAAIVADWTGIP
AGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTARAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQ
NLVTINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHV
DFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETSRGVIARLHLDRLVA
RMGEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQHILPQLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFETTSQ
EGICQKS

Sequences:

>Translated_887_residues
METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWGELTHWLHQLTQQPDNDILHVLRHYQIPLSDVEKALLRQLDMLP
AGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLLALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPE
AQERPYDGSGLASAIPGESSQAIPNGVQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGE
AGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAGRSPQPVILFVDEVHTLVGAG
GASGTGDAANLLKPALACGTLRTIGATTWSEYKRHIEKDPALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILD
EAVRAAVQLSHRYIPARQLPDKAISLLDTAAVRVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERCDALT
ARIFSLNDELTASESRWQRELELVHTLQELRVAESDADDKTTLQQAETALREWQGDAPVVFPEVSAAIVAAIVADWTGIP
AGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTARAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQ
NLVTINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHV
DFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETSRGVIARLHLDRLVA
RMGEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQHILPQLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFETTSQ
EGICQKS
>Mature_887_residues
METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWGELTHWLHQLTQQPDNDILHVLRHYQIPLSDVEKALLRQLDMLP
AGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLLALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPE
AQERPYDGSGLASAIPGESSQAIPNGVQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGE
AGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAGRSPQPVILFVDEVHTLVGAG
GASGTGDAANLLKPALACGTLRTIGATTWSEYKRHIEKDPALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILD
EAVRAAVQLSHRYIPARQLPDKAISLLDTAAVRVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERCDALT
ARIFSLNDELTASESRWQRELELVHTLQELRVAESDADDKTTLQQAETALREWQGDAPVVFPEVSAAIVAAIVADWTGIP
AGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTARAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQ
NLVTINMSEFQEAHTVSTLKGAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHV
DFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETSRGVIARLHLDRLVA
RMGEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQHILPQLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFETTSQ
EGICQKS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=306, Percent_Identity=32.3529411764706, Blast_Score=156, Evalue=1e-37,
Organism=Escherichia coli, GI1788943, Length=691, Percent_Identity=41.9681620839363, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1787109, Length=248, Percent_Identity=46.7741935483871, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6320464, Length=698, Percent_Identity=39.541547277937, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6323002, Length=691, Percent_Identity=34.876989869754, Blast_Score=396, Evalue=1e-110,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR017729
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96783; Mature: 96783

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00870 CLPAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWGELTHWLHQLTQQPDNDILHVLRH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
YQIPLSDVEKALLRQLDMLPAGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLL
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHEEECCCHHCCCEEEE
ALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPEAQERPYDGSGLASAIPGESS
EECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCC
QAIPNGVQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECC
AGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAG
CCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
RSPQPVILFVDEVHTLVGAGGASGTGDAANLLKPALACGTLRTIGATTWSEYKRHIEKDP
CCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
ALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILDEAVRAAVQLSHRYIPARQLP
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCHHHCC
DKAISLLDTAAVRVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERCDALT
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ARIFSLNDELTASESRWQRELELVHTLQELRVAESDADDKTTLQQAETALREWQGDAPVV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEE
FPEVSAAIVAAIVADWTGIPAGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTA
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQNLVTINMSEFQEAHTVSTLK
HCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHC
GAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHV
CCCCCCCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYL
EECCEEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCEEECC
PLDETSRGVIARLHLDRLVARMGEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HILPQLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFETTSQEGICQKS
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
METPVSRSALYGKLAGPLFRSLESATAFCKLRSNPWGELTHWLHQLTQQPDNDILHVLRH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
YQIPLSDVEKALLRQLDMLPAGASAISDFSHHIDLSVEKAWMLASVRYGDNKIRSGWLLL
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHEEECCCHHCCCEEEE
ALLTTPELRRVLSSICAPLATLPVDELTEILPSLIETSPEAQERPYDGSGLASAIPGESS
EECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCC
QAIPNGVQDGKSALAKYCQDMTAQARDGKIDPVTGREHEIRTMTDILLRRRQNNPLLTGE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECC
AGVGKTAVVEGFALAIAQGEVPPALREVRLLALDVGALLAGASMKGEFESRLKGLLEEAG
CCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCC
RSPQPVILFVDEVHTLVGAGGASGTGDAANLLKPALACGTLRTIGATTWSEYKRHIEKDP
CCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCH
ALTRRFQVLQIAEPEEIPAMEMVRGLVDTLEKHHNVLILDEAVRAAVQLSHRYIPARQLP
HHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCHHHCC
DKAISLLDTAAVRVALTLHTPPASVQFLRQQLKAAEMERSLLQKQEKMGIQSDERCDALT
HHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ARIFSLNDELTASESRWQRELELVHTLQELRVAESDADDKTTLQQAETALREWQGDAPVV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEE
FPEVSAAIVAAIVADWTGIPAGRMVKDEASQVLELPARLAQRVTGQDGALAQIGERIQTA
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAGLGDPRKPVGVFMLAGPSGVGKTETALALAEAIYGGEQNLVTINMSEFQEAHTVSTLK
HCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHC
GAPPGYVGYGEGGVLTEAVRRHPWSVVLLDEIEKAHHDVHELFYQVFDKGGMEDGEGTHV
CCCCCCCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
DFKNTTLLLTTNVGSDLISQMCEDPALMPDATGLKEALMPELRKHFPAAFLGRVTVIPYL
EECCEEEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCEEECC
PLDETSRGVIARLHLDRLVARMGEQHGVTLTYSEELVAHIVACCPMHETGARLLIGYIEQ
CCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHH
HILPQLSRYWLQAMTEKAAIRQIDIGVNGDEQIVFETTSQEGICQKS
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10986262 [H]