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The map label for this gene is fepA [H]

Identifier: 29142682

GI number: 29142682

Start: 2353169

End: 2355424

Strand: Direct

Name: fepA [H]

Synonym: t2283

Alternate gene names: 29142682

Gene position: 2353169-2355424 (Clockwise)

Preceding gene: 29142678

Following gene: 29142683

Centisome position: 49.11

GC content: 54.08

Gene sequence:

>2256_bases
ATGAACAAGAAGATTCATTCCCTGACCTTACTGGTCAATTTAGGGATTTACGGGGCCGCTCTGCCCGTGATGGCGGAAGA
AAAAACCGATAGCGCTGCGCTCACCAATGAAGACACCATCGTGGTGACCGCTGCCCAGCAAAACCTACAGGCGCCAGGCG
TATCCACCATCACCGCCGATGAGATCCGCAAAAATCCGCCCGCGCGCGACGTGTCAGAAATCATCCGCACCATGCCGGGC
GTAAACCTGACCGGCAACTCCACCAGCGGTCAGCGCGGGAATAACCGCCAAATCGATATTCGCGGCATGGGGCCGGAAAA
CACCCTTATCCTGATAGACGGTAAACCCGTTACCAGCCGTAACTCGGTACGCCTGGGCTGGCGCGGCGAACGCGATACCC
GCGGCGATACCGCGTGGGTGCCGCCAGAGATGATCGAACGCATTGAAGTGCTGCGTGGCCCTGCCGCCGCACGTTACGGT
AACGGCGCGGCGGGTGGCGTGGTCAATATCATTACCAAAAAAGGCGGTAGCGAGTGGCACGGTTCCTGGAATACCTATTT
CAACGCGCCGGAACATAAAGACGAAGGCGCGACCAAACGCACTAACTTTAGCCTGAACGGCCCGCTGGGCGGGGATTTCA
GCTTCCGTCTGTATGGCAACCTCGACAAAACGCAGGCGGATGCGCGTAATATTAACCAGGGGCATCAGTCTGAACGTACC
GGGAGTTACGCCGACACGTTACCAGCCGGACGCGAGGGGGTCATTAACAAAGATATCAACGGCGTGGTTCGTTGGGATTT
TGCTCCGCTTCAGTCTCTGGAGCTGGAGGCCGGTTACAGTCGCCAGGGCAACCTCTATGCCGGCGATACTCAGAACACCA
ATACGAACCAACTGGTGAAAGATAATTACGGAAAAGAGACGAACCGCCTCTATCGGCAGAACTACTCTCTGACCTGGAAC
GGCGGCTGGGATAATGGCGTAACCACCAGTAACTGGGTGCAATACGAACATACCCGTAACTCCCGGATGCCGGAAGGCCT
GGCAGGCGGCACCGAAGGGATTTTCGATCCCAAAGCATCGCAAAAATATGCCGATGCCGATTTGAATGACGTCACCCTAC
ACAGCGAAGTGAGCCTTCCTTTCGATCTGCTGGTCAATCAAAACCTGACGCTGGGTACCGAATGGGCCCAACAGCGTATG
AAAGACCAGCTCTCCAACTCGCAAACCTTTATGGGGGGAAATATCCCGGGTTACAGCAGTACCAATCGTAGCCCCTATTC
AAAAGCGGAAATTTTCTCTCTGTTTGCTGAAAACAACATGGAACTGACCGACAGCACCATGCTGACGCCGGGGATACGTT
TCGATCATCACAGTATTGTGGGGGATAACTGGAGCCCGTCGCTGAACCTGTCGCAAGGTCTGGGGGATGACTTTACCCTG
AAAATGGGGATCGCCCGCGCTTATAAAGCCCCGAGTCTGTACCAGACTAACCCAAACTATATTTTGTACAGTAAAGGTCA
GGGTTGCTACGCGACTGGCGCGGGTACCGGCATCGGCTGCTATATGATGGGTAACGATGATCTGAAAGCCGAAACCAGCA
TCAATAAAGAGATCGGTCTGGAATTTAAACGCGATGGCTGGCTGGCCGGCGTCACCTGGTTCCGCAATGATTACCGCAAT
AAGATTGAAGCGGGTACGGTGCCGTTGCAGCGTATCAATAACGGCAAAACGGATGTCTATCAGTGGGAGAACGTGCCGAA
AGCGGTCGTTGAAGGTCTGGAAGGCACGTTAAACGTACCGGTTAGCGACACCGTCAACTGGACGAATAACGTTACGTATA
TGCTGCAGAGTAAAAACAAAGAGACCGGCGAACGCCTGTCGATTATTCCACAGTACACGCTTAACTCTACCCTGAGCTGG
CAGGTACGTCAGGACGTTTCTCTGCAATCGACCTTCACCTGGTACGGCAAACAAGAGCCGAAGAAATATGATTATCAGGG
TAACCCCGTTACCGGAACAGATAAGCAAGCGGTCAGCCCATACAGTATTGTTGGCCTGAGCGCGACCTGGGACGTGACCA
AAAATGTCAGCCTGACCGGTGGCGTGGATAACCTCTTTGACAAACGTCTGTGGCGTGAAGGTAACGCGCAAACCGTCAGG
GATACGCAAACTGGCGCTTATATGGCGGGCGCTGGCGCGTACACCTATAACGAACCGGGACGTACGTGGTATATGAGCAT
TAATACCCATTTCTGA

Upstream 100 bases:

>100_bases
TATTGTAGCGCTATGGGACGCGCGAACACAATTTCACCACCCGGCCAATGCCTTTGACGGGCGCTTTGGCTTATGTGGCT
AAAGAAAAGCAGGATATACA

Downstream 100 bases:

>100_bases
TGGCAACCTTCCCTCCCTCATTCGGGGAGGGAATTAGCAAAAGACGATGCTGACATCTCATTTTCCCCTTCCCTTTGCCG
GACACAGGCTGCATATCGTC

Product: outer membrane receptor FepA

Products: NA

Alternate protein names: Enterobactin outer-membrane receptor [H]

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MNKKIHSLTLLVNLGIYGAALPVMAEEKTDSAALTNEDTIVVTAAQQNLQAPGVSTITADEIRKNPPARDVSEIIRTMPG
VNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSRNSVRLGWRGERDTRGDTAWVPPEMIERIEVLRGPAAARYG
NGAAGGVVNIITKKGGSEWHGSWNTYFNAPEHKDEGATKRTNFSLNGPLGGDFSFRLYGNLDKTQADARNINQGHQSERT
GSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYAGDTQNTNTNQLVKDNYGKETNRLYRQNYSLTWN
GGWDNGVTTSNWVQYEHTRNSRMPEGLAGGTEGIFDPKASQKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRM
KDQLSNSQTFMGGNIPGYSSTNRSPYSKAEIFSLFAENNMELTDSTMLTPGIRFDHHSIVGDNWSPSLNLSQGLGDDFTL
KMGIARAYKAPSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRN
KIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVPVSDTVNWTNNVTYMLQSKNKETGERLSIIPQYTLNSTLSW
QVRQDVSLQSTFTWYGKQEPKKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTGGVDNLFDKRLWREGNAQTVR
DTQTGAYMAGAGAYTYNEPGRTWYMSINTHF

Sequences:

>Translated_751_residues
MNKKIHSLTLLVNLGIYGAALPVMAEEKTDSAALTNEDTIVVTAAQQNLQAPGVSTITADEIRKNPPARDVSEIIRTMPG
VNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSRNSVRLGWRGERDTRGDTAWVPPEMIERIEVLRGPAAARYG
NGAAGGVVNIITKKGGSEWHGSWNTYFNAPEHKDEGATKRTNFSLNGPLGGDFSFRLYGNLDKTQADARNINQGHQSERT
GSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYAGDTQNTNTNQLVKDNYGKETNRLYRQNYSLTWN
GGWDNGVTTSNWVQYEHTRNSRMPEGLAGGTEGIFDPKASQKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRM
KDQLSNSQTFMGGNIPGYSSTNRSPYSKAEIFSLFAENNMELTDSTMLTPGIRFDHHSIVGDNWSPSLNLSQGLGDDFTL
KMGIARAYKAPSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRN
KIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVPVSDTVNWTNNVTYMLQSKNKETGERLSIIPQYTLNSTLSW
QVRQDVSLQSTFTWYGKQEPKKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTGGVDNLFDKRLWREGNAQTVR
DTQTGAYMAGAGAYTYNEPGRTWYMSINTHF
>Mature_751_residues
MNKKIHSLTLLVNLGIYGAALPVMAEEKTDSAALTNEDTIVVTAAQQNLQAPGVSTITADEIRKNPPARDVSEIIRTMPG
VNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSRNSVRLGWRGERDTRGDTAWVPPEMIERIEVLRGPAAARYG
NGAAGGVVNIITKKGGSEWHGSWNTYFNAPEHKDEGATKRTNFSLNGPLGGDFSFRLYGNLDKTQADARNINQGHQSERT
GSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYAGDTQNTNTNQLVKDNYGKETNRLYRQNYSLTWN
GGWDNGVTTSNWVQYEHTRNSRMPEGLAGGTEGIFDPKASQKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRM
KDQLSNSQTFMGGNIPGYSSTNRSPYSKAEIFSLFAENNMELTDSTMLTPGIRFDHHSIVGDNWSPSLNLSQGLGDDFTL
KMGIARAYKAPSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRN
KIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVPVSDTVNWTNNVTYMLQSKNKETGERLSIIPQYTLNSTLSW
QVRQDVSLQSTFTWYGKQEPKKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTGGVDNLFDKRLWREGNAQTVR
DTQTGAYMAGAGAYTYNEPGRTWYMSINTHF

Specific function: This protein is involved in the initial step of iron uptake by binding ferrienterobactin (Fe-ENT), an iron chelatin siderophore that allows E.coli to extract iron from the environment. FepA also acts as a receptor for colicins B and D [H]

COG id: COG4771

COG function: function code P; Outer membrane receptor for ferrienterochelin and colicins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tonB-dependent receptor family [H]

Homologues:

Organism=Escherichia coli, GI1786798, Length=755, Percent_Identity=81.8543046357616, Blast_Score=1269, Evalue=0.0,
Organism=Escherichia coli, GI1788478, Length=768, Percent_Identity=32.1614583333333, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790405, Length=241, Percent_Identity=31.5352697095436, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787723, Length=168, Percent_Identity=30.3571428571429, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917
- InterPro:   IPR010105 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 83013; Mature: 83013

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00430 TONB_DEPENDENT_REC_1 ; PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIHSLTLLVNLGIYGAALPVMAEEKTDSAALTNEDTIVVTAAQQNLQAPGVSTITAD
CCCCEEEEEEEEECCHHHHCCCEEECCCCCCCEECCCCEEEEEECCCCCCCCCCCEEEHH
EIRKNPPARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSR
HHHCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCC
NSVRLGWRGERDTRGDTAWVPPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGGSEWH
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEECCCCCCCC
GSWNTYFNAPEHKDEGATKRTNFSLNGPLGGDFSFRLYGNLDKTQADARNINQGHQSERT
CCCCCEECCCCCCCCCCCEECCEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCC
GSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYAGDTQNTNTNQLVK
CCHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCCCHHH
DNYGKETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHTRNSRMPEGLAGGTEGIFDPKAS
CCCCCHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
QKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRMKDQLSNSQTFMGGNIPGYSS
CCCCCCCCCCEEEECCCCCCEEHEECCCCEECHHHHHHHHHHHHCCCCEEECCCCCCCCC
TNRSPYSKAEIFSLFAENNMELTDSTMLTPGIRFDHHSIVGDNWSPSLNLSQGLGDDFTL
CCCCCCHHHHHHHHHHCCCCEECCCEEECCCCEECCCEECCCCCCCCCCHHCCCCCCEEE
KMGIARAYKAPSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGL
EEEHHHHHCCCCEEECCCCEEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHCC
EFKRDGWLAGVTWFRNDYRNKIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVP
EECCCCEEEEHHHHHHHHHCHHCCCCCCHHHCCCCCCCEEECCCCCHHHHHCCCCEEECC
VSDTVNWTNNVTYMLQSKNKETGERLSIIPQYTLNSTLSWQVRQDVSLQSTFTWYGKQEP
CCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCC
KKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTGGVDNLFDKRLWREGNAQTVR
CCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCCCEEE
DTQTGAYMAGAGAYTYNEPGRTWYMSINTHF
ECCCCCEEECCCCEEECCCCCEEEEEEECCC
>Mature Secondary Structure
MNKKIHSLTLLVNLGIYGAALPVMAEEKTDSAALTNEDTIVVTAAQQNLQAPGVSTITAD
CCCCEEEEEEEEECCHHHHCCCEEECCCCCCCEECCCCEEEEEECCCCCCCCCCCEEEHH
EIRKNPPARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPENTLILIDGKPVTSR
HHHCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCC
NSVRLGWRGERDTRGDTAWVPPEMIERIEVLRGPAAARYGNGAAGGVVNIITKKGGSEWH
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEEEEECCCCCCCC
GSWNTYFNAPEHKDEGATKRTNFSLNGPLGGDFSFRLYGNLDKTQADARNINQGHQSERT
CCCCCEECCCCCCCCCCCEECCEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCC
GSYADTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYSRQGNLYAGDTQNTNTNQLVK
CCHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCEEECCCCCCCCCCHHH
DNYGKETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHTRNSRMPEGLAGGTEGIFDPKAS
CCCCCHHHHHHHHCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
QKYADADLNDVTLHSEVSLPFDLLVNQNLTLGTEWAQQRMKDQLSNSQTFMGGNIPGYSS
CCCCCCCCCCEEEECCCCCCEEHEECCCCEECHHHHHHHHHHHHCCCCEEECCCCCCCCC
TNRSPYSKAEIFSLFAENNMELTDSTMLTPGIRFDHHSIVGDNWSPSLNLSQGLGDDFTL
CCCCCCHHHHHHHHHHCCCCEECCCEEECCCCEECCCEECCCCCCCCCCHHCCCCCCEEE
KMGIARAYKAPSLYQTNPNYILYSKGQGCYATGAGTGIGCYMMGNDDLKAETSINKEIGL
EEEHHHHHCCCCEEECCCCEEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCCCHHHCC
EFKRDGWLAGVTWFRNDYRNKIEAGTVPLQRINNGKTDVYQWENVPKAVVEGLEGTLNVP
EECCCCEEEEHHHHHHHHHCHHCCCCCCHHHCCCCCCCEEECCCCCHHHHHCCCCEEECC
VSDTVNWTNNVTYMLQSKNKETGERLSIIPQYTLNSTLSWQVRQDVSLQSTFTWYGKQEP
CCCCCCCCCCEEEEEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCC
KKYDYQGNPVTGTDKQAVSPYSIVGLSATWDVTKNVSLTGGVDNLFDKRLWREGNAQTVR
CCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCEEEECCHHHHHHHHHHCCCCCCEEE
DTQTGAYMAGAGAYTYNEPGRTWYMSINTHF
ECCCCCEEECCCCEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3015941; 8905232; 9278503; 2974033; 2526281; 2201687; 9886293 [H]