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The map label for this gene is mrdB [H]

Identifier: 29142629

GI number: 29142629

Start: 2294302

End: 2295414

Strand: Direct

Name: mrdB [H]

Synonym: t2228

Alternate gene names: 29142629

Gene position: 2294302-2295414 (Clockwise)

Preceding gene: 29142628

Following gene: 29142630

Centisome position: 47.88

GC content: 53.01

Gene sequence:

>1113_bases
ATGACGGATAATCCGAACAAAAAAACCTTCTGGGATAAAATTCATATCGATCCCACGATGCTACTCATTCTGCTGGCGTT
ACTGGTTTATAGCGCGTTGGTCATCTGGAGCGCCAGCGGCCAGGACATCGGCATGATGGAGCGTAAAATCGGTCAGATTG
CGATGGGGCTGGTGGTTATGGTGGTGATGGCGCAGATCCCGCCGCGCGTGTATGAAGGCTGGGCGCCCTATCTCTATATC
ATTTGTATCATTCTGCTGGTCGCCGTTGATGCCTTCGGCGCAATATCCAAGGGCGCGCAGCGCTGGCTCGATCTGGGAAT
CGTTCGCTTTCAGCCTTCGGAAATAGCCAAAATCGCCGTGCCGCTGATGGTCGCCCGCTTTATTAACCGCGACGTCTGCC
CGCCCTCGCTGAAAAATACCGCCATCGCCCTGGTATTAATCTTTATGCCTACCCTGCTGGTCGCCGCCCAGCCGGACCTG
GGGACGTCTATTCTGGTCGCCCTTTCCGGTCTCTTTGTCCTATTTTTGTCTGGCCTGAGCTGGCGCTTAATTGGGGTCGC
CATTGTATTGATCGCTGCATTTATTCCTATTTTGTGGTTTTTCCTGATGCACGATTACCAACGCCAGCGCGTCATGATGC
TCCTTGATCCGGAGACCGATCCGCTGGGCGCGGGCTATCATATTATTCAGTCTAAAATCGCCATTGGCTCAGGCGGCCTG
CGCGGTAAAGGCTGGCTCCACGGCACCCAGTCGCAGCTGGAGTTTTTACCCGAACGCCATACCGACTTTATTTTCGCGGT
ACTGGCGGAAGAGCTCGGTCTGGTGGGCATCCTGATTCTGCTCGCGCTCTATATTTTGCTAATAATGCGCGGTCTGTGGA
TTGCCGCCAGAGCGCAAACTACATTTGGTCGCGTGATGGCTGGCGGATTAATGTTAATATTATTCGTTTATGTCTTCGTA
AATATTGGTATGGTGAGCGGTATTCTGCCTGTGGTTGGAGTACCTTTACCCTTGGTCAGCTACGGGGGCTCCGCACTGAT
TGTGCTGATGGCCGGGTTCGGGATTGTGATGTCGATCCACACCCACAGAAAAATGTTGTCGAAAAGCGTATAA

Upstream 100 bases:

>100_bases
CGATCATGCGCCAAATCCTTGATCACATCATGCTGGGCGACAACAACACCCATTTGCCTGCGGAAAACCCGGTGGTTGCA
GCGGCGGAGGACCAATAATC

Downstream 100 bases:

>100_bases
GGGGTGCGCAATGCATAAGCAGTTGCCTGTAATCTGCGTCGCGGCAGGGATAGTACTACTCGCGGCATGTACTAATGACG
GCGGTCAGCAGCAAACCACC

Product: cell wall shape-determining protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTDNPNKKTFWDKIHIDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVVMVVMAQIPPRVYEGWAPYLYI
ICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
GTSILVALSGLFVLFLSGLSWRLIGVAIVLIAAFIPILWFFLMHDYQRQRVMMLLDPETDPLGAGYHIIQSKIAIGSGGL
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV

Sequences:

>Translated_370_residues
MTDNPNKKTFWDKIHIDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVVMVVMAQIPPRVYEGWAPYLYI
ICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
GTSILVALSGLFVLFLSGLSWRLIGVAIVLIAAFIPILWFFLMHDYQRQRVMMLLDPETDPLGAGYHIIQSKIAIGSGGL
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFV
NIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV
>Mature_369_residues
TDNPNKKTFWDKIHIDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVVMVVMAQIPPRVYEGWAPYLYII
CIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLG
TSILVALSGLFVLFLSGLSWRLIGVAIVLIAAFIPILWFFLMHDYQRQRVMMLLDPETDPLGAGYHIIQSKIAIGSGGLR
GKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVN
IGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV

Specific function: This is one of the proteins responsible for the rod shape of E.coli. Is required for the expression of the enzymatic activity of PBP2, which is thought to synthesize peptidoglycan in the step of initiation of cell elongation [H]

COG id: COG0772

COG function: function code D; Bacterial cell division membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ftsW/rodA/spoVE family [H]

Homologues:

Organism=Escherichia coli, GI1786853, Length=370, Percent_Identity=98.1081081081081, Blast_Score=724, Evalue=0.0,
Organism=Escherichia coli, GI1786277, Length=366, Percent_Identity=30.8743169398907, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001182
- InterPro:   IPR018365
- InterPro:   IPR011923 [H]

Pfam domain/function: PF01098 FTSW_RODA_SPOVE [H]

EC number: NA

Molecular weight: Translated: 40533; Mature: 40402

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00428 FTSW_RODA_SPOVE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNPNKKTFWDKIHIDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVVM
CCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAV
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLGTSILVALSGLFVLFLSGLS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGVAIVLIAAFIPILWFFLMHDYQRQRVMMLLDPETDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKSV
HHHHHHHCCC
>Mature Secondary Structure 
TDNPNKKTFWDKIHIDPTMLLILLALLVYSALVIWSASGQDIGMMERKIGQIAMGLVVM
CCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
VVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRWLDLGIVRFQPSEIAKIAV
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHH
PLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDLGTSILVALSGLFVLFLSGLS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCH
WRLIGVAIVLIAAFIPILWFFLMHDYQRQRVMMLLDPETDPLGAGYHIIQSKIAIGSGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCC
RGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVGILILLALYILLIMRGLWIAARAQT
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGVPLPLVSYGGSALIVLMAGFGIVMSIH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHH
THRKMLSKSV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]