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The map label for this gene is nac [H]

Identifier: 29142591

GI number: 29142591

Start: 2248806

End: 2249732

Strand: Direct

Name: nac [H]

Synonym: t2183

Alternate gene names: 29142591

Gene position: 2248806-2249732 (Clockwise)

Preceding gene: 29142590

Following gene: 29142592

Centisome position: 46.93

GC content: 56.31

Gene sequence:

>927_bases
ATGGAGCTTCGCCAGTTACGCTATTTCGTGCGTATTATTGAAACCGGCAGTATGGGCAGCGCCGCACAGGATCTTGATAT
TGGCGTTTCAGCATTGAGTCAACAGATGTCCCGTCTGGAAAACGAGCTCGCCATCCGTCTGCTGCAGCGCACCTCGCGCG
GCGTAACGCCAACCAACGCCGGGCTGGCCTTTTATTCACAGGCGCAACTCGCCCTGCGTCATGCCGACGACGCGATTCTC
GCCGCCAGAGAAGCGCGCCTTTCCGGTCATGTGAGCGTAGGAATGGCCCCCAGTACCGCCTCCATACTGGGTATACCGTT
TATCCATGCCATGCAGGAAAACTATGCCGATGTCCGACTGCATGTGGTGGAAAGCTTGTCCGGTAATCTGGAAAGAATGA
TCAATACCCGCCAGATTGATTTAGCCGTTGTCTTTCAAAAAGATAAAATCCTGCGCTGGAGCGCCAGACCGATCCTTGAA
GAACAGCTTTTTCTGATTGGCTCCCATGCCCTGCTGGCGGCGCTTCCTGATAACCCGATCACACCTGAACAACTGGCAGG
CATTCCGCTCATTATGCCAAGCCAGGGACATGGGCTGCGCGGCAGGCTGGACGCTGTCTGCAAGGAACATGCGCTTAATG
TTGAAATCGTCGCGGAAATTGACGGCCTGGCGTTGTTGATGCGCGCCGTGCGCGACGGTCTTGGCGCCACGTTGCAGCCC
GGCGCGGCGATTTCTCATCTGGATAACGACGCGCTACGCGTGATCGGCGTCCATAATCCGGTACTCAGCCGCCCCAATTT
TTTAGTCAGCCTGTCGGATGATGAACTGACGCCCGCCGGCCTTGCCGCCAGAGTAGTCCTTACCAAAGTTATGCGTCAGT
TAGTCGACGCCGGTGAATGGCCAGGCGCAACCCTTTACGCTTACTAA

Upstream 100 bases:

>100_bases
AAGAGCCAACCGCCCGGTTGGCTTTTTTATATCCTCCTCTTTCCTGCCGCTTGATGTTGATTTAATGTAAAAAACGCTAT
CGCATCTCAGGAGAGCAGGA

Downstream 100 bases:

>100_bases
ACCCCTGTTTAGCGCTACCTGATAGCGCGCCGCTTATTTATCCGTATACATTTCAATTACAAATTTAACAAATAGTTAAA
TGGTACGGGAGTATAAGATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELRQLRYFVRIIETGSMGSAAQDLDIGVSALSQQMSRLENELAIRLLQRTSRGVTPTNAGLAFYSQAQLALRHADDAIL
AAREARLSGHVSVGMAPSTASILGIPFIHAMQENYADVRLHVVESLSGNLERMINTRQIDLAVVFQKDKILRWSARPILE
EQLFLIGSHALLAALPDNPITPEQLAGIPLIMPSQGHGLRGRLDAVCKEHALNVEIVAEIDGLALLMRAVRDGLGATLQP
GAAISHLDNDALRVIGVHNPVLSRPNFLVSLSDDELTPAGLAARVVLTKVMRQLVDAGEWPGATLYAY

Sequences:

>Translated_308_residues
MELRQLRYFVRIIETGSMGSAAQDLDIGVSALSQQMSRLENELAIRLLQRTSRGVTPTNAGLAFYSQAQLALRHADDAIL
AAREARLSGHVSVGMAPSTASILGIPFIHAMQENYADVRLHVVESLSGNLERMINTRQIDLAVVFQKDKILRWSARPILE
EQLFLIGSHALLAALPDNPITPEQLAGIPLIMPSQGHGLRGRLDAVCKEHALNVEIVAEIDGLALLMRAVRDGLGATLQP
GAAISHLDNDALRVIGVHNPVLSRPNFLVSLSDDELTPAGLAARVVLTKVMRQLVDAGEWPGATLYAY
>Mature_308_residues
MELRQLRYFVRIIETGSMGSAAQDLDIGVSALSQQMSRLENELAIRLLQRTSRGVTPTNAGLAFYSQAQLALRHADDAIL
AAREARLSGHVSVGMAPSTASILGIPFIHAMQENYADVRLHVVESLSGNLERMINTRQIDLAVVFQKDKILRWSARPILE
EQLFLIGSHALLAALPDNPITPEQLAGIPLIMPSQGHGLRGRLDAVCKEHALNVEIVAEIDGLALLMRAVRDGLGATLQP
GAAISHLDNDALRVIGVHNPVLSRPNFLVSLSDDELTPAGLAARVVLTKVMRQLVDAGEWPGATLYAY

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=35.6275303643725, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787879, Length=314, Percent_Identity=29.9363057324841, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=282, Percent_Identity=25.886524822695, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=23.9583333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790399, Length=252, Percent_Identity=25.7936507936508, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788748, Length=282, Percent_Identity=25.886524822695, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787601, Length=250, Percent_Identity=29.2, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788481, Length=237, Percent_Identity=23.6286919831224, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788887, Length=253, Percent_Identity=26.8774703557312, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33389; Mature: 33389

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRQLRYFVRIIETGSMGSAAQDLDIGVSALSQQMSRLENELAIRLLQRTSRGVTPTNA
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GLAFYSQAQLALRHADDAILAAREARLSGHVSVGMAPSTASILGIPFIHAMQENYADVRL
CEEHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCHHHHH
HVVESLSGNLERMINTRQIDLAVVFQKDKILRWSARPILEEQLFLIGSHALLAALPDNPI
HHHHHHCCCHHHHHCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHCCCEEEEECCCCCC
TPEQLAGIPLIMPSQGHGLRGRLDAVCKEHALNVEIVAEIDGLALLMRAVRDGLGATLQP
CHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCC
GAAISHLDNDALRVIGVHNPVLSRPNFLVSLSDDELTPAGLAARVVLTKVMRQLVDAGEW
CCHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PGATLYAY
CCCEEEEC
>Mature Secondary Structure
MELRQLRYFVRIIETGSMGSAAQDLDIGVSALSQQMSRLENELAIRLLQRTSRGVTPTNA
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GLAFYSQAQLALRHADDAILAAREARLSGHVSVGMAPSTASILGIPFIHAMQENYADVRL
CEEHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHCCCHHHHH
HVVESLSGNLERMINTRQIDLAVVFQKDKILRWSARPILEEQLFLIGSHALLAALPDNPI
HHHHHHCCCHHHHHCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHCCCEEEEECCCCCC
TPEQLAGIPLIMPSQGHGLRGRLDAVCKEHALNVEIVAEIDGLALLMRAVRDGLGATLQP
CHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCC
GAAISHLDNDALRVIGVHNPVLSRPNFLVSLSDDELTPAGLAARVVLTKVMRQLVDAGEW
CCHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PGATLYAY
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]