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The map label for this gene is potE [H]

Identifier: 29142583

GI number: 29142583

Start: 2241703

End: 2243022

Strand: Direct

Name: potE [H]

Synonym: t2175

Alternate gene names: 29142583

Gene position: 2241703-2243022 (Clockwise)

Preceding gene: 29142582

Following gene: 29142584

Centisome position: 46.78

GC content: 54.24

Gene sequence:

>1320_bases
ATGAGTAAACCTAAATCCAATAAAATGGGTGTCGTGCAGCTCACAATTCTGACTATGGTCAACATGATGGGCTCCGGGAT
CATCATGCTACCGACCAAACTGTCTGAAGTCGGCACGATATCCATTATCTCCTGGCTGGTCACCGCCGTCGGCTCGATGG
CGCTGGCCTGGGCGTTTGCGAAATGCGGTATGTTTAGCCGTAAATCCGGCGGCATGGGCGGTTACGCCGAATATGCCTTC
GGTAAATCCGGCAACTTTATGGCGAACTATACCTACGGCGTCTCGCTGCTGATCGCCAACGTGGCTATCGCCATCTCCGC
TGTCGGCTATGGTACCGAACTGTTCGGCGCCGCGCTGACGCCGCTCCAGATCGGTCTGGCGACTATCGCCGTATTGTGGG
TTTGTACCATCGCAAACTTTGGCGGCGCGCGTATTACCGGACAGATCAGTAGCGTTACCGTTTGGGGCGTCATCATTCCG
GTTGTCGGCCTGTGCATCATTGGCTGGTTCTGGTTTAGCCCCAGCATGTACGTAGCGTCATGGAACCCGCACCATGTTCC
GTTCTTTAGCGCGGTCGGCTCTTCCATCGCCATGACGTTGTGGGCATTTCTTGGACTGGAATCCGCCTGCGCTAACGCAG
ACGTGGTCGAGAATCCGGAAAAGAACGTCCCCATCGCGGTACTGGGCGGTACGCTGGGCGCGGCGGTCATCTATATCGTT
TCGACTAACGTGATTGCCGGGATTGTGCCGAACATGGATCTGGCGAACTCCACGGCGCCGTTTGGTCTGGCGTTTGCACA
GATGTTCACGCCTGCGGTAGGTAAAGTGATCATGGCGCTGATGGTAATGTCCTGCTGCGGCTCGCTGCTGGGCTGGCAGT
TCACTATCGCCCAGGTCTTTAAATCCTCCGCTGACGAAGGCTATTTCCCGAAAGTCTTCTCTCGTGTAACGAAAGTCGAC
GCGCCGGTACAGGGGATGCTGATTATTGTGATTATTCAGACTGGCCTGTCGCTGATGACCATTAGCCCGTCGCTGAATAG
TCAGTTTAACGTCCTGGTTAACCTGGCGGTGGTGACGAACATTATTCCGTATATTCTGTCGATGGCCGCGCTGGTTATCA
TTCAGAAAATGGCGAATGTGCCATCATCAAAAGCGAAAGTCGCTAACTTCGTGGCCTTCGTTGGCGCCATGTATAGCTTC
TACGCGCTATATTCCTCCGGTGAAGAAGCCATGCTGTACGGCTCAATTGTGACCTTCCTCGGCTGGACGTTATACGGCCT
GGTCTCACCGCGTTTTGAATTGAAAAATAAACACGGCTGA

Upstream 100 bases:

>100_bases
AGGGCGTTTATATCGAAGAACACGACGGCCGTAAGCAGGTCTGGTGCTACGTCATTAAACCTCGTGACGCGCAGCGCTCC
CTGCTGAAGGAGGAAAAATT

Downstream 100 bases:

>100_bases
TGGCGCGCCCGGGCTGCGGTAACGATAGCCCGGGCATTTTCACTCTTTGAATAAGATAATAGAAATGAGTAATCCAGAAC
GGGAAGTCACTGATATAGCA

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSKPKSNKMGVVQLTILTMVNMMGSGIIMLPTKLSEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAF
GKSGNFMANYTYGVSLLIANVAIAISAVGYGTELFGAALTPLQIGLATIAVLWVCTIANFGGARITGQISSVTVWGVIIP
VVGLCIIGWFWFSPSMYVASWNPHHVPFFSAVGSSIAMTLWAFLGLESACANADVVENPEKNVPIAVLGGTLGAAVIYIV
STNVIAGIVPNMDLANSTAPFGLAFAQMFTPAVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKVFSRVTKVD
APVQGMLIIVIIQTGLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKMANVPSSKAKVANFVAFVGAMYSF
YALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG

Sequences:

>Translated_439_residues
MSKPKSNKMGVVQLTILTMVNMMGSGIIMLPTKLSEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAF
GKSGNFMANYTYGVSLLIANVAIAISAVGYGTELFGAALTPLQIGLATIAVLWVCTIANFGGARITGQISSVTVWGVIIP
VVGLCIIGWFWFSPSMYVASWNPHHVPFFSAVGSSIAMTLWAFLGLESACANADVVENPEKNVPIAVLGGTLGAAVIYIV
STNVIAGIVPNMDLANSTAPFGLAFAQMFTPAVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKVFSRVTKVD
APVQGMLIIVIIQTGLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKMANVPSSKAKVANFVAFVGAMYSF
YALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG
>Mature_438_residues
SKPKSNKMGVVQLTILTMVNMMGSGIIMLPTKLSEVGTISIISWLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFG
KSGNFMANYTYGVSLLIANVAIAISAVGYGTELFGAALTPLQIGLATIAVLWVCTIANFGGARITGQISSVTVWGVIIPV
VGLCIIGWFWFSPSMYVASWNPHHVPFFSAVGSSIAMTLWAFLGLESACANADVVENPEKNVPIAVLGGTLGAAVIYIVS
TNVIAGIVPNMDLANSTAPFGLAFAQMFTPAVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSADEGYFPKVFSRVTKVDA
PVQGMLIIVIIQTGLSLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKMANVPSSKAKVANFVAFVGAMYSFY
ALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=439, Percent_Identity=94.0774487471526, Blast_Score=836, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=437, Percent_Identity=33.4096109839817, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1790575, Length=411, Percent_Identity=31.8734793187348, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787890, Length=423, Percent_Identity=27.4231678486998, Blast_Score=108, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17540018, Length=312, Percent_Identity=25.9615384615385, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46476; Mature: 46344

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPKSNKMGVVQLTILTMVNMMGSGIIMLPTKLSEVGTISIISWLVTAVGSMALAWAFA
CCCCCCCCCCCEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELFGAALT
HCCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PLQIGLATIAVLWVCTIANFGGARITGQISSVTVWGVIIPVVGLCIIGWFWFSPSMYVAS
HHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
WNPHHVPFFSAVGSSIAMTLWAFLGLESACANADVVENPEKNVPIAVLGGTLGAAVIYIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
STNVIAGIVPNMDLANSTAPFGLAFAQMFTPAVGKVIMALMVMSCCGSLLGWQFTIAQVF
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSSADEGYFPKVFSRVTKVDAPVQGMLIIVIIQTGLSLMTISPSLNSQFNVLVNLAVVTN
HCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
IIPYILSMAALVIIQKMANVPSSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GWTLYGLVSPRFELKNKHG
HHHHHHHCCCCHHCCCCCC
>Mature Secondary Structure 
SKPKSNKMGVVQLTILTMVNMMGSGIIMLPTKLSEVGTISIISWLVTAVGSMALAWAFA
CCCCCCCCCCEEHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVAIAISAVGYGTELFGAALT
HCCCCCCCCCCCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PLQIGLATIAVLWVCTIANFGGARITGQISSVTVWGVIIPVVGLCIIGWFWFSPSMYVAS
HHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
WNPHHVPFFSAVGSSIAMTLWAFLGLESACANADVVENPEKNVPIAVLGGTLGAAVIYIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHH
STNVIAGIVPNMDLANSTAPFGLAFAQMFTPAVGKVIMALMVMSCCGSLLGWQFTIAQVF
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSSADEGYFPKVFSRVTKVDAPVQGMLIIVIIQTGLSLMTISPSLNSQFNVLVNLAVVTN
HCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHH
IIPYILSMAALVIIQKMANVPSSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GWTLYGLVSPRFELKNKHG
HHHHHHHCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]