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The map label for this gene is rimO [H]

Identifier: 29142458

GI number: 29142458

Start: 2093889

End: 2095214

Strand: Direct

Name: rimO [H]

Synonym: t2037

Alternate gene names: 29142458

Gene position: 2093889-2095214 (Clockwise)

Preceding gene: 29142455

Following gene: 29142464

Centisome position: 43.7

GC content: 53.92

Gene sequence:

>1326_bases
ATGAGTAATGTAACCCACCAGCCAAAGATCGGCTTCGTTTCCCTGGGCTGTCCGAAAAACCTCGTTGATTCGGAACGAAT
CCTGACTGAGCTGCGTACCGAAGGCTATGACGTTGTGCCACACTATGACGATGCGGATATGGTTATCGTTAATACCTGTG
GCTTTATTGACAGCGCGGTACAAGAGTCACTGGAAGCCATTGGCGAAGCGCTGAACGAAAACGGTAAGGTAATCGTCACC
GGCTGCCTGGGCGCAAAAGAAGATCAGATCCGCGAAGTGCATCCGAAAGTACTGGAAATCACCGGGCCGCATAGCTATGA
GCAAGTTCTGCAGCACGTACATCATTACGTACCGAAGCCGAAGCACAATCCATTTTTAAGCCTCGTACCGGAACAGGGTG
TAAAACTGACGCCGCGCCATTATGCGTATCTGAAAATTTCTGAAGGCTGTAATCACCGCTGCACGTTCTGCATCATCCCG
TCGATGCGCGGGGATCTGGTCAGCCGTCCGATTGGCGACGTATTAAGCGAAGCGAAACGTCTGGTTGATGCCGGGGTCAA
AGAGATTCTGGTGATTTCGCAAGATACCTCCGCCTACGGCGTAGATGTGAAGCACCGCACCGGATTCCATAATGGCGAAC
CGATGAAAACCAGTATGGTGAGCCTGTGCGAGCAGTTATCAAAACTGGGCGTCTGGACACGTCTGCACTACGTCTACCCC
TACCCACACGTTGATGATGTGATTCCGCTAATGGCGGAAGGCAAAATCCTGCCTTATCTGGACATTCCGCTGCAACATGC
CAGCCCGCGGATTCTTAAACTGATGAAACGTCCTGGTTCCGTTGACCGCCAGCTGGCGCGCATTAAGCAGTGGCGCGAAA
TCTGCCCGGAACTGACCCTACGCTCCACCTTTATTGTCGGCTTCCCCGGCGAAACGGAAGAAGACTTCCAGATGCTGCTC
GACTTCCTGAAAGAGGCGCGCCTCGATCGTGTAGGCTGCTTCAAGTACAGCCCGGTGGAAGGCGCAGGCGCGAATGAGCT
GCCGGATCAGGTGCCGGAAGAGGTGAAAGAAGAGCGCTGGAACCGCTTTATGCAACTGCAACAGCAAATCTCCGCTGAGC
GCTTGCAGGAAAAAGTGGGCCGCGAAATTCTGGTGATCGTCGATGAGGTTGACGAAGAAGGCGCTATCGGGCGTAGCATG
GCGGATGCGCCGGAAATTGACGGCGCGGTCTATTTGAACGGCGAAACCAACGTCAAGCCGGGCGATATCGTGCGCGTGAA
AGTGGAAAACGCCGACGAATATGATTTGTGGGGCAGTCGGGTTTAA

Upstream 100 bases:

>100_bases
TTGATGCCGCCGCTCCTGATGAGATTGCGATACAATTTTTGTTCGGATATCTGTAGAATCCCTGCCCTGACCATTCCAAT
AAATGAACACTTTTTAAGCT

Downstream 100 bases:

>100_bases
CGCCACTTCCCCCGGCGCGGGTGCCGAATGTGTTACCCCACCTGGCGAGCCGGTTCCAGGCCCGCCAGGCGTCAACGCCA
CCGGGCTGGCTTACCCCTTA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSNVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPHYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVT
GCLGAKEDQIREVHPKVLEITGPHSYEQVLQHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIGDVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPMKTSMVSLCEQLSKLGVWTRLHYVYP
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLL
DFLKEARLDRVGCFKYSPVEGAGANELPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIVDEVDEEGAIGRSM
ADAPEIDGAVYLNGETNVKPGDIVRVKVENADEYDLWGSRV

Sequences:

>Translated_441_residues
MSNVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPHYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVT
GCLGAKEDQIREVHPKVLEITGPHSYEQVLQHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIGDVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPMKTSMVSLCEQLSKLGVWTRLHYVYP
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLL
DFLKEARLDRVGCFKYSPVEGAGANELPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIVDEVDEEGAIGRSM
ADAPEIDGAVYLNGETNVKPGDIVRVKVENADEYDLWGSRV
>Mature_440_residues
SNVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPHYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTG
CLGAKEDQIREVHPKVLEITGPHSYEQVLQHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPS
MRGDLVSRPIGDVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPMKTSMVSLCEQLSKLGVWTRLHYVYPY
PHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLD
FLKEARLDRVGCFKYSPVEGAGANELPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIVDEVDEEGAIGRSMA
DAPEIDGAVYLNGETNVKPGDIVRVKVENADEYDLWGSRV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=26.3157894736842, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=26.6503667481663, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1787057, Length=441, Percent_Identity=97.2789115646258, Blast_Score=885, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=385, Percent_Identity=28.3116883116883, Blast_Score=151, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=26.0215053763441, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71996771, Length=212, Percent_Identity=29.7169811320755, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=24.8953974895398, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19922432, Length=412, Percent_Identity=26.2135922330097, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49610; Mature: 49479

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPHYDDADMVIVNTCGFIDSAV
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCHHHHHH
QESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLQHVHHYVPKP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCC
KHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLSEAKR
CCCCCEEECCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHH
LVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPMKTSMVSLCEQLSKLGVWTRLHYVYP
HHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTL
CCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
RSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGAGANELPDQVPEEVKEERW
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
NRFMQLQQQISAERLQEKVGREILVIVDEVDEEGAIGRSMADAPEIDGAVYLNGETNVKP
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GDIVRVKVENADEYDLWGSRV
CCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SNVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPHYDDADMVIVNTCGFIDSAV
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCHHHHHH
QESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLQHVHHYVPKP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCC
KHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLSEAKR
CCCCCEEECCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHH
LVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPMKTSMVSLCEQLSKLGVWTRLHYVYP
HHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTL
CCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
RSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGAGANELPDQVPEEVKEERW
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
NRFMQLQQQISAERLQEKVGREILVIVDEVDEEGAIGRSMADAPEIDGAVYLNGETNVKP
HHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GDIVRVKVENADEYDLWGSRV
CCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA