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The map label for this gene is amiD [H]

Identifier: 29142423

GI number: 29142423

Start: 2062860

End: 2063690

Strand: Reverse

Name: amiD [H]

Synonym: t2002

Alternate gene names: 29142423

Gene position: 2063690-2062860 (Counterclockwise)

Preceding gene: 29142424

Following gene: 29142415

Centisome position: 43.07

GC content: 57.4

Gene sequence:

>831_bases
ATGAAAGCGCTACTGTGGCTGGTTGGTCTCGCGTTGCTGTTAACAGGCTGCGCGAGCGAAAAAGGAATTATCGATAAAGA
GGGATATCAGCTTGATACCCGACATCGGGCGCAGGCGGCCTATCCGCGCATTAAAGTCCTGGTGATTCACTATACGGCGG
AAAACTTTGACGTTTCGCTGGCGACGTTAACGGGTCGCAACGTCAGTTCGCATTACCTGATTCCCGCAACCCCGCCATTA
TATGGCGGTAAACCGCGCATCTGGCAACTGGTGCCGGAACAGGATCAGGCTTGGCATGCGGGCGTCAGTTTCTGGCGAGG
CGCCACGCGTCTCAATGATACGTCTATTGGCATTGAGCTGGAAAATCGCGGCTGGCGAATGTCCGGCGGGGTGAAATCTT
TCGCGCCGTTTGAATCCGCGCAAATTCAGGCATTGATCCCGTTAGCGAAGGACATTATCGCGCGCTATGACATCAAACCG
CAGAATGTGGTGGCCCATGCGGATATCGCGCCGCAGCGTAAAGACGATCCCGGCCCGCGCTTCCCGTGGCGCGAGCTGGC
GGCACAGGGGATTGGCGCCTGGCCTGACGCCCAGCGTGTGGCGTTTTATCTGGCTGGACGCGCGCCGTATACGCCAGTCG
ATACCGCAACGGTGCTTGCGTTACTCTCGCGCTATGGCTATGAAGTCAAAGCCGATATGACGGCGCGCGAGCAACAGCGG
GTGATTATGGCGTTCCAGATGCACTTCCGTCCGGCGCAATGGAACGGTATCGCAGATGCCGAAACGCAGGCGATTGCCGA
AGCATTACTGGAGAAGTACGGCCAGGATTAA

Upstream 100 bases:

>100_bases
CCGATGCGGTGGTAGGCATCGATATTGATTACGAAACTGTCGGCAAGGATGGCAGTATGCTGATGGTCAGCGTCAGCGGA
ACGGCGGTGAAAACGCGCCG

Downstream 100 bases:

>100_bases
CGCGGTAAATTTCCGTGATCGCGCAGCCAGGCGGCGGTGCGCTCAATGCCTTCGTCCAGCGTGACGATGGGCTGGTAGCC
CAGCTCGTCCTGCGCGCGAG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSLATLTGRNVSSHYLIPATPPL
YGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIELENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKP
QNVVAHADIAPQRKDDPGPRFPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD

Sequences:

>Translated_276_residues
MKALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSLATLTGRNVSSHYLIPATPPL
YGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIELENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKP
QNVVAHADIAPQRKDDPGPRFPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD
>Mature_276_residues
MKALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSLATLTGRNVSSHYLIPATPPL
YGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIELENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKP
QNVVAHADIAPQRKDDPGPRFPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=276, Percent_Identity=81.5217391304348, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1786300, Length=124, Percent_Identity=40.3225806451613, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30738; Mature: 30738

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHCCCEEEEEEEEEECCCCEEEE
ATLTGRNVSSHYLIPATPPLYGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIEL
EEEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
ENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKPQNVVAHADIAPQRKDDPGPR
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCC
FPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
CCHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHH
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD
HHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKALLWLVGLALLLTGCASEKGIIDKEGYQLDTRHRAQAAYPRIKVLVIHYTAENFDVSL
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHCCCEEEEEEEEEECCCCEEEE
ATLTGRNVSSHYLIPATPPLYGGKPRIWQLVPEQDQAWHAGVSFWRGATRLNDTSIGIEL
EEEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEE
ENRGWRMSGGVKSFAPFESAQIQALIPLAKDIIARYDIKPQNVVAHADIAPQRKDDPGPR
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCC
FPWRELAAQGIGAWPDAQRVAFYLAGRAPYTPVDTATVLALLSRYGYEVKADMTAREQQR
CCHHHHHHCCCCCCCCHHEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHH
VIMAFQMHFRPAQWNGIADAETQAIAEALLEKYGQD
HHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]