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The map label for this gene is acT [C]

Identifier: 29142403

GI number: 29142403

Start: 2040284

End: 2041195

Strand: Reverse

Name: acT [C]

Synonym: t1981

Alternate gene names: 29142403

Gene position: 2041195-2040284 (Counterclockwise)

Preceding gene: 29142409

Following gene: 29142397

Centisome position: 42.6

GC content: 58.11

Gene sequence:

>912_bases
ATGATAAAAACGGATCTCAATGATTTTGCGTGGTTTGTGCATGTGGTCGAAGAGGGTGGGTTTGCCGCGGCGGGGCGTGC
GCTTGATGAACCAAAATCAAAACTGAGCCGGCGTATTGCGCAACTGGAGGAACGGCTGGGCGTGCGATTGATTCAGCGAA
CCACCCGGCAGTTTAACGTTACCGAAGTGGGGCAAACATTCTATGAGCACTGTAAAGCGATGCTGGTGGAAGCTCAGGCG
GCGCAGGATGCCATTGCCGCATTGCAGGTAGAGCCTCGCGGTATCGTGAAGCTAACCTGCCCGGTGACGCTGCTGCATGT
ACACATCGGCCCAATGCTGGCGAAGTTTATGGCGCGTTATCCTGATGTTTCGCTACAGCTTGAAGCGACCAATCGTCGGG
TGGACGTCGTAGGGGAGGGGGTGGATGTGGCGATTCGCGTCAGGCCGCGCCCGTTTGAAGATAGCGACCTGGTGATGCGC
GTCCTGGCTGACAGAGGGCATCGCCTGTTTGCCAGCCCGGATCTCATCGCACGGATGGGAATCCCTTCCGCGCCGGCGGA
GTTGAGCCATTGGCCAGGGCTAAGTCTTGCATCCGGCAAGCATATCCACCGCTGGGAACTTTACGGCCCGCAGGGCGCGC
GTGCGGAAGTCCATTTTACCCCGCGTATGATTACTACAGACATGTTGGCGCTACGCGAGGCGGCGATGGCGGGCGTGGGG
CTTGTGCAGTTACCTGTCTTAATGGTGAAAGAACAACTGGCCGCCGGTGAACTGGTCGCGGTGCTGGAAGAGTGGGAGCC
CCGGCGGGAGGTTATTCATGCTGTGTTCCCATCCCGGCGAGGGCTATTACCTTCCGTACGTGCGTTGGTCGATTTTTTGA
CGGAAGAGTATGCGCGGATGGTGGAAGAGTAA

Upstream 100 bases:

>100_bases
CCTCAGTGGCGTTGTCTTGATGTGAATAAGCTTATATTCATGACAAAATGTTCGGTAGTGGGTAAAATGGCTCATACCGT
TCCATTAATAGGACAATGTA

Downstream 100 bases:

>100_bases
CCTGCTCGTCATTCATCGACTTAATGGACGCAAAAAAGGCCGGTTAAACCGACCTTTTATCCAGCCGCCCTTCAGGGCGT
GAGGAGACAATTAGCGACTA

Product: transcriptional activator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIKTDLNDFAWFVHVVEEGGFAAAGRALDEPKSKLSRRIAQLEERLGVRLIQRTTRQFNVTEVGQTFYEHCKAMLVEAQA
AQDAIAALQVEPRGIVKLTCPVTLLHVHIGPMLAKFMARYPDVSLQLEATNRRVDVVGEGVDVAIRVRPRPFEDSDLVMR
VLADRGHRLFASPDLIARMGIPSAPAELSHWPGLSLASGKHIHRWELYGPQGARAEVHFTPRMITTDMLALREAAMAGVG
LVQLPVLMVKEQLAAGELVAVLEEWEPRREVIHAVFPSRRGLLPSVRALVDFLTEEYARMVEE

Sequences:

>Translated_303_residues
MIKTDLNDFAWFVHVVEEGGFAAAGRALDEPKSKLSRRIAQLEERLGVRLIQRTTRQFNVTEVGQTFYEHCKAMLVEAQA
AQDAIAALQVEPRGIVKLTCPVTLLHVHIGPMLAKFMARYPDVSLQLEATNRRVDVVGEGVDVAIRVRPRPFEDSDLVMR
VLADRGHRLFASPDLIARMGIPSAPAELSHWPGLSLASGKHIHRWELYGPQGARAEVHFTPRMITTDMLALREAAMAGVG
LVQLPVLMVKEQLAAGELVAVLEEWEPRREVIHAVFPSRRGLLPSVRALVDFLTEEYARMVEE
>Mature_303_residues
MIKTDLNDFAWFVHVVEEGGFAAAGRALDEPKSKLSRRIAQLEERLGVRLIQRTTRQFNVTEVGQTFYEHCKAMLVEAQA
AQDAIAALQVEPRGIVKLTCPVTLLHVHIGPMLAKFMARYPDVSLQLEATNRRVDVVGEGVDVAIRVRPRPFEDSDLVMR
VLADRGHRLFASPDLIARMGIPSAPAELSHWPGLSLASGKHIHRWELYGPQGARAEVHFTPRMITTDMLALREAAMAGVG
LVQLPVLMVKEQLAAGELVAVLEEWEPRREVIHAVFPSRRGLLPSVRALVDFLTEEYARMVEE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=30.5084745762712, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=29.1095890410959, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=28.4745762711864, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=30.976430976431, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=27.7966101694915, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787589, Length=284, Percent_Identity=28.8732394366197, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=27.4914089347079, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI157672245, Length=142, Percent_Identity=33.8028169014084, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=24.5674740484429, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786448, Length=301, Percent_Identity=24.2524916943522, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=148, Percent_Identity=31.7567567567568, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33831; Mature: 33831

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTDLNDFAWFVHVVEEGGFAAAGRALDEPKSKLSRRIAQLEERLGVRLIQRTTRQFNV
CCCCCHHHHHHHHHHHHCCCCHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TEVGQTFYEHCKAMLVEAQAAQDAIAALQVEPRGIVKLTCPVTLLHVHIGPMLAKFMARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCEEEEHHHHHHHHHHHHHHC
PDVSLQLEATNRRVDVVGEGVDVAIRVRPRPFEDSDLVMRVLADRGHRLFASPDLIARMG
CCCEEEEEECCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCHHHHHCC
IPSAPAELSHWPGLSLASGKHIHRWELYGPQGARAEVHFTPRMITTDMLALREAAMAGVG
CCCCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHCCC
LVQLPVLMVKEQLAAGELVAVLEEWEPRREVIHAVFPSRRGLLPSVRALVDFLTEEYARM
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
VEE
HCC
>Mature Secondary Structure
MIKTDLNDFAWFVHVVEEGGFAAAGRALDEPKSKLSRRIAQLEERLGVRLIQRTTRQFNV
CCCCCHHHHHHHHHHHHCCCCHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TEVGQTFYEHCKAMLVEAQAAQDAIAALQVEPRGIVKLTCPVTLLHVHIGPMLAKFMARY
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCEEEEHHHHHHHHHHHHHHC
PDVSLQLEATNRRVDVVGEGVDVAIRVRPRPFEDSDLVMRVLADRGHRLFASPDLIARMG
CCCEEEEEECCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCHHHHHCC
IPSAPAELSHWPGLSLASGKHIHRWELYGPQGARAEVHFTPRMITTDMLALREAAMAGVG
CCCCCCHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHCCC
LVQLPVLMVKEQLAAGELVAVLEEWEPRREVIHAVFPSRRGLLPSVRALVDFLTEEYARM
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
VEE
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]