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The map label for this gene is ompF

Identifier: 29142359

GI number: 29142359

Start: 1979688

End: 1980779

Strand: Direct

Name: ompF

Synonym: t1935

Alternate gene names: 29142359

Gene position: 1979688-1980779 (Clockwise)

Preceding gene: 29142358

Following gene: 29142360

Centisome position: 41.31

GC content: 51.1

Gene sequence:

>1092_bases
ATGATGAAGCGCAAAATCCTGGCAGCGGTGATCCCTGCCCTGCTGGCTGCTGCAACCGCAAACGCAGCAGAAATTTATAA
TAAAGATGGTAATAAGCTGGATCTGTACGGTAAAGCCGTGGGTCGTCACGTATGGACAACGACCGGCGATAGTAAAAATG
CCGACCAGACTTATGCCCAGATTGGTTTTAAAGGGGAAACGCAGATTAACACCGATCTGACCGGTTTCGGTCAGTGGGAA
TACCGTACTAAAGCAGACCGCGCTGAAGGCGAACAGCAGAACTCGAATCTGGTACGTCTGGCTTTCGCGGGTTTGAAATA
CGCGGAAGTGGGTTCAATCGATTATGGTCGTAACTACGGTATCGTTTACGATGTTGAATCCTATACCGATATGGCCCCCT
ACTTCTCCGGCGAAACCTGGGGCGGCGCCTATACCGATAACTACATGACCAGCCGTGCTGGCGGTTTGTTGACCTACCGT
AACTCTGACTTCTTTGGTCTGGTGGACGGTCTCTCTTTCGGTATCCAGTATCAGGGTAAAAATCAGGACAACCACAGCAT
TAACTCTCAGAATGGCGATGGCGTAGGTTACACCATGGCATATGAGTTCGACGGCTTTGGCGTCACCGCAGCGTACAGCA
ACAGCAAGCGTACTAACGATCAGCAGGATCGCGATGGTAATGGCGATCGCGCAGAATCGTGGGCCGTTGGCGCGAAATAT
GATGCAAACAACGTCTACCTGGCTGCCGTATATGCTGAAACCCGCAATATGAGCATTGTTGAAAATACGGTTACCGATAC
CGTGGAAATGGCAAACAAAACGCAGAACCTGGAAGTGGTCGCTCAGTACCAGTTTGACTTCGGCCTGCGTCCGGCAATCT
CGTATGTGCAGAGTAAAGGTAAGCAGTTGAACGGCGCCGACGGCTCGGCCGATCTGGCGAAATATATTCAGGCGGGCGCG
ACTTACTACTTCAACAAAAACATGAACGTATGGGTTGACTACCGTTTCAACCTGCTGGACGAAAACGACTACAGCTCCAG
CTACGTTGGCACCGACGATCAGGCGGCTGTCGGTATTACTTACCAGTTCTGA

Upstream 100 bases:

>100_bases
ACACCAAACTCTCATCAATATTTCTGTAAAGTTTTATTGACAGAATTTATTGACGGCAGTGGCAGGTGTCATATAAAAAA
ACCAATGAGGGTAATAAATA

Downstream 100 bases:

>100_bases
TCAGCAGACCCCTTTGTTTTATGCCTCAAAAACAGGACTTCGGTCCTGTTTTTTTATCTCTCTTAGAGAAAATGTGCGTC
TTTCAAAAACAGGCGGGCTT

Product: outer membrane protein F

Products: NA

Alternate protein names: Outer membrane protein S3; Porin ompF

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQIGFKGETQINTDLTGFGQWE
YRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYGIVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYR
NSDFFGLVDGLSFGIQYQGKNQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKGKQLNGADGSADLAKYIQAGA
TYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGITYQF

Sequences:

>Translated_363_residues
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQIGFKGETQINTDLTGFGQWE
YRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYGIVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYR
NSDFFGLVDGLSFGIQYQGKNQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKGKQLNGADGSADLAKYIQAGA
TYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGITYQF
>Mature_363_residues
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQIGFKGETQINTDLTGFGQWE
YRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYGIVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYR
NSDFFGLVDGLSFGIQYQGKNQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKGKQLNGADGSADLAKYIQAGA
TYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGITYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family

Homologues:

Organism=Escherichia coli, GI1787160, Length=369, Percent_Identity=58.5365853658537, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1788544, Length=378, Percent_Identity=52.9100529100529, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786436, Length=366, Percent_Identity=54.6448087431694, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787641, Length=384, Percent_Identity=53.3854166666667, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 1980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 17600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12744 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): OMPF_SALTI (Q56113)

Other databases:

- EMBL:   X89757
- EMBL:   AL627268
- EMBL:   AE014613
- RefSeq:   NP_455485.1
- RefSeq:   NP_805701.1
- ProteinModelPortal:   Q56113
- SMR:   Q56113
- GeneID:   1067105
- GeneID:   1247431
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t1935
- KEGG:   sty:STY1002
- HOGENOM:   HBG673855
- OMA:   RMALSES
- ProtClustDB:   CLSK894015
- BioCyc:   SENT209261:T1935-MONOMER
- BioCyc:   SENT220341:STY1002-MONOMER
- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702
- Gene3D:   G3DSA:2.40.160.10
- PRINTS:   PR00183
- PRINTS:   PR00182

Pfam domain/function: PF00267 Porin_1

EC number: NA

Molecular weight: Translated: 40106; Mature: 40106

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb7816dc)-; HASH(0xeee4734)-; HASH(0xcb1f380)-; HASH(0xdf12764)-; HASH(0xdf0f428)-; HASH(0xf5a42e0)-; HASH(0xdf1061c)-; HASH(0xf234ea0)-; HASH(0xdf129b0)-; HASH(0xdf101c0)-; HASH(0xdf13b44)-; HASH(0xdf13544)-; HASH(0xdf081ec)-; HASH(0xd60b074)-; HASH(0xdf12ea8)-; HASH(0xdf10748)-; HASH(0xc3fe330)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQ
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEHHHHCCEEEEECCCCCCCCHHHHH
IGFKGETQINTDLTGFGQWEYRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYG
CCCCCCCEECCCCCCCCCEEEECCCHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCC
IVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYRNSDFFGLVDGLSFGIQYQGK
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCEEEECCCEEEEEECCC
NQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCEE
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKG
CCCCEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCC
KQLNGADGSADLAKYIQAGATYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGIT
CCCCCCCCHHHHHHHHHCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEE
YQF
EEH
>Mature Secondary Structure
MMKRKILAAVIPALLAAATANAAEIYNKDGNKLDLYGKAVGRHVWTTTGDSKNADQTYAQ
CCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEEHHHHCCEEEEECCCCCCCCHHHHH
IGFKGETQINTDLTGFGQWEYRTKADRAEGEQQNSNLVRLAFAGLKYAEVGSIDYGRNYG
CCCCCCCEECCCCCCCCCEEEECCCHHCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCC
IVYDVESYTDMAPYFSGETWGGAYTDNYMTSRAGGLLTYRNSDFFGLVDGLSFGIQYQGK
EEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCEEEECCCEEEEEECCC
NQDNHSINSQNGDGVGYTMAYEFDGFGVTAAYSNSKRTNDQQDRDGNGDRAESWAVGAKY
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCEE
DANNVYLAAVYAETRNMSIVENTVTDTVEMANKTQNLEVVAQYQFDFGLRPAISYVQSKG
CCCCEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCC
KQLNGADGSADLAKYIQAGATYYFNKNMNVWVDYRFNLLDENDYSSSYVGTDDQAAVGIT
CCCCCCCCHHHHHHHHHCCEEEEEECCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEE
YQF
EEH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504