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The map label for this gene is spiA [H]

Identifier: 29141723

GI number: 29141723

Start: 1334869

End: 1336362

Strand: Direct

Name: spiA [H]

Synonym: t1262

Alternate gene names: 29141723

Gene position: 1334869-1336362 (Clockwise)

Preceding gene: 29141722

Following gene: 29141724

Centisome position: 27.86

GC content: 42.3

Gene sequence:

>1494_bases
ATGGTAGTAAATAAACGTTTAATCTTAATTTTACTATTTATACTCAATACAGCAAAGAGTGATGAGTTATCATGGAAAGG
TAATGACTTCACCCTTTATGCCAGACAAATGCCATTAGCAGAGGTTTTACATCTGCTCTCAGAGAACTATGATACGGCTA
TTACTATTAGCCCATTGATAACAGCTACATTTAGTGGAAAAATTCCGCCTGGACCACCGGTCGATATTTTGAATAACCTG
GCAGCACAATATGATTTGCTAACCTGGTTTGATGGCAGCATGTTATATGTATATCCTGCATCGTTATTAAAACATCAGGT
TATCACTTTCAATATTTTATCTACTGGACGGTTCATTCATTACTTACGCAGCCAGAATATCCTTTCATCACCGGGATGCG
AGGTTAAAGAAATTACCGGTACCAGAGCTGTGGAGGTGAGTGGTGTTCCCAGCTGTCTGACTCGTATTAGTCAATTAGCT
TCAGTGCTGGATAATGCGTTAATCAAACGAAAAGACAGTGCGGTGAGTGTAAGTATATACACGCTTAAGTATGCCACTGC
GATGGATACCCAATACCAATATCGCGATCAGTCCGTCGTGGTTCCAGGGGTCGTGAGTGTATTGCGTGAGATGAGTAAAA
CCAGCGTACCGGCGTCATCGACGACCAATGGTTCACCCGCTACACAGGCATTGCCCATGTTTGCTGCCGACCCACGCCAG
AATGCAGTGATCGTTCGTGATTATGCGGCCAATATGGCCGGGTATCGGAAACTCATCACAGAATTAGATCAACGCCAGCA
GATGATAGAGATTTCGGTGAAAATTATCGATGTTAATGCTGGAGATATTAACCAGTTAGGCATCGACTGGGGAACGGCAG
TGTCGCTGGGTGGCAAGAAAATTGCGTTCAATACAGGTTTGAATGACGGTGGTGCTAGCGGTTTTTCAACGGTAATCAGC
GATACCTCAAACTTTATGGTGCGTTTGAATGCCCTGGAAAAAAGCTCTCAGGCTTATGTACTTTCCCAGCCATCTGTGGT
GACTTTAAATAATATCCAGGCTGTGCTGGATAAAAATATTACTTTCTATACCAAACTGCAGGGAGAAAAAGTGGCTAAAC
TTGAATCCATCACTACGGGTTCTTTGTTGCGCGTTACACCTCGCTTGTTAAATGACAATGGCACGCAAAAAATAATGCTT
AATCTTAATATTCAGGATGGACAACAAAGTGATACGCAAAGCGAAACAGATCCGCTACCCGAAGTGCAAAATTCTGAAAT
TGCTTCGCAAGCCACATTATTGGCCGGACAAAGTCTATTGCTGGGAGGGTTTAAACAAGGTAAACAAATCCACTCGCAAA
ACAAAATCCCTTTATTGGGCGATATCCCTGTCGTAGGTCATTTGTTTCGCAATGATACGACTCAAGTACATAGTGTAATC
AGGCTTTTTTTGATTAAAGCCTCAGTAGTAAATAATGGCATATCTCATGGTTAA

Upstream 100 bases:

>100_bases
GAGCTTATTGCTATTGAGATAGAAAAACACCTGGCTTTAACTTGCATTTTAAAAAATGTAATACGCAATCACCATAAACT
TTATTCGGGTGGGGTATAAA

Downstream 100 bases:

>100_bases
TCCAAAGAGTTCCTGGAAAATACGTTTTTTAGGTCACGTTTTACAAGGCCGGGAAGTATGGCTGAATGAAGGTAACCTGT
CACTGGGGGAGAAGGGATGC

Product: outer membrane secretory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MVVNKRLILILLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLITATFSGKIPPGPPVDILNNL
AAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIHYLRSQNILSSPGCEVKEITGTRAVEVSGVPSCLTRISQLA
SVLDNALIKRKDSAVSVSIYTLKYATAMDTQYQYRDQSVVVPGVVSVLREMSKTSVPASSTTNGSPATQALPMFAADPRQ
NAVIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGGKKIAFNTGLNDGGASGFSTVIS
DTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVLDKNITFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIML
NLNIQDGQQSDTQSETDPLPEVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQVHSVI
RLFLIKASVVNNGISHG

Sequences:

>Translated_497_residues
MVVNKRLILILLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLITATFSGKIPPGPPVDILNNL
AAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIHYLRSQNILSSPGCEVKEITGTRAVEVSGVPSCLTRISQLA
SVLDNALIKRKDSAVSVSIYTLKYATAMDTQYQYRDQSVVVPGVVSVLREMSKTSVPASSTTNGSPATQALPMFAADPRQ
NAVIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGGKKIAFNTGLNDGGASGFSTVIS
DTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVLDKNITFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIML
NLNIQDGQQSDTQSETDPLPEVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQVHSVI
RLFLIKASVVNNGISHG
>Mature_497_residues
MVVNKRLILILLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLITATFSGKIPPGPPVDILNNL
AAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIHYLRSQNILSSPGCEVKEITGTRAVEVSGVPSCLTRISQLA
SVLDNALIKRKDSAVSVSIYTLKYATAMDTQYQYRDQSVVVPGVVSVLREMSKTSVPASSTTNGSPATQALPMFAADPRQ
NAVIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGGKKIAFNTGLNDGGASGFSTVIS
DTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVLDKNITFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIML
NLNIQDGQQSDTQSETDPLPEVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQVHSVI
RLFLIKASVVNNGISHG

Specific function: Very likely required for the export process of the yop proteins [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=251, Percent_Identity=25.8964143426295, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI87082242, Length=384, Percent_Identity=24.4791666666667, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR004845
- InterPro:   IPR003522 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 54099; Mature: 54099

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVNKRLILILLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLI
CCCCHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCEE
TATFSGKIPPGPPVDILNNLAAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIH
EEEECCCCCCCCCHHHHHHHHHHHCEEEEECCCEEEEEEHHHHCCEEEEEEEECCCHHHH
YLRSQNILSSPGCEVKEITGTRAVEVSGVPSCLTRISQLASVLDNALIKRKDSAVSVSIY
HHHCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
TLKYATAMDTQYQYRDQSVVVPGVVSVLREMSKTSVPASSTTNGSPATQALPMFAADPRQ
EEEEEHHCCCCCEECCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEECCCCC
NAVIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGGKK
CEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCEECCCCCCEEECCCEE
IAFNTGLNDGGASGFSTVISDTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVLDKNI
EEEECCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCEEEECCCCEEEECCHHHHHCCCC
TFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIMLNLNIQDGQQSDTQSETDPLP
EEEEEECCCHHHHHHHCCCCCCEEECHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
EVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQVHSVI
CCCCCHHHHHHHHHCCCHHHCCCHHHCHHHHCCCCCCEEECCCHHHHHHCCCHHHHHHHH
RLFLIKASVVNNGISHG
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVVNKRLILILLFILNTAKSDELSWKGNDFTLYARQMPLAEVLHLLSENYDTAITISPLI
CCCCHHHHHHHHHHHCCCCCCCCEECCCCEEEEEECCCHHHHHHHHHCCCCCEEEECCEE
TATFSGKIPPGPPVDILNNLAAQYDLLTWFDGSMLYVYPASLLKHQVITFNILSTGRFIH
EEEECCCCCCCCCHHHHHHHHHHHCEEEEECCCEEEEEEHHHHCCEEEEEEEECCCHHHH
YLRSQNILSSPGCEVKEITGTRAVEVSGVPSCLTRISQLASVLDNALIKRKDSAVSVSIY
HHHCCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
TLKYATAMDTQYQYRDQSVVVPGVVSVLREMSKTSVPASSTTNGSPATQALPMFAADPRQ
EEEEEHHCCCCCEECCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEECCCCC
NAVIVRDYAANMAGYRKLITELDQRQQMIEISVKIIDVNAGDINQLGIDWGTAVSLGGKK
CEEEEEHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCEECCCCCCEEECCCEE
IAFNTGLNDGGASGFSTVISDTSNFMVRLNALEKSSQAYVLSQPSVVTLNNIQAVLDKNI
EEEECCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCEEEECCCCEEEECCHHHHHCCCC
TFYTKLQGEKVAKLESITTGSLLRVTPRLLNDNGTQKIMLNLNIQDGQQSDTQSETDPLP
EEEEEECCCHHHHHHHCCCCCCEEECHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
EVQNSEIASQATLLAGQSLLLGGFKQGKQIHSQNKIPLLGDIPVVGHLFRNDTTQVHSVI
CCCCCHHHHHHHHHCCCHHHCCCHHHCHHHHCCCCCCEEECCCHHHHHHCCCHHHHHHHH
RLFLIKASVVNNGISHG
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1860816 [H]