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The map label for this gene is spiC

Identifier: 29141722

GI number: 29141722

Start: 1334484

End: 1334867

Strand: Direct

Name: spiC

Synonym: t1261

Alternate gene names: 29141722

Gene position: 1334484-1334867 (Clockwise)

Preceding gene: 29141717

Following gene: 29141723

Centisome position: 27.85

GC content: 41.15

Gene sequence:

>384_bases
ATGCTGGCAGTTTTAAAAGGCATTCCATTAATTCAGGATATCAGGGCCGAAGGTAATAGCCGATCCTGGATAATGACTAT
TGATGGGCATCCTGCCAGAGGAGAAATTTTCTCAGAAGCATTTTCTATTTCTTTGTTCTTAAATGACCTGGAAAGCTTAC
CTAAGCCTTGTCTTGCCTATGTGACACTACTGCTTGCAGCACACCCGGACGTCCATGACTATGCTATACAGCTCACAGCG
GATGGGGGATGGTTAAACGGTTATTATACCACAAGTAGTAGCTCTGAGCTTATTGCTATTGAGATAGAAAAACACCTGGC
TTTAACTTGCATTTTAAAAAATGTAATACGCAATCACCATAAACTTTATTCGGGTGGGGTATAA

Upstream 100 bases:

>100_bases
CAAGAAGAGTATAGTCGAAATAAATGTGAATCAGGCTTTTTACGGATGTGGTTGTGAGCGAATTTGATAGAAACTCCCAT
TTATGTCTGAGGAGGGATTC

Downstream 100 bases:

>100_bases
AATGGTAGTAAATAAACGTTTAATCTTAATTTTACTATTTATACTCAATACAGCAAAGAGTGATGAGTTATCATGGAAAG
GTAATGACTTCACCCTTTAT

Product: pathogenicity island secreted effector protein

Products: NA

Alternate protein names: Secretion system apparatus protein B

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAYVTLLLAAHPDVHDYAIQLTA
DGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHHKLYSGGV

Sequences:

>Translated_127_residues
MLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAYVTLLLAAHPDVHDYAIQLTA
DGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHHKLYSGGV
>Mature_127_residues
MLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAYVTLLLAAHPDVHDYAIQLTA
DGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHHKLYSGGV

Specific function: Virulence protein that plays a central role in mammalian macrophage infection, by inhibiting phagosome-lysosome fusion and cellular trafficking. May act by disrupting the function of the mammalian HOOK3 protein, a protein involved in the cellular traffic

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Cytoplasm. Note=Translocated into the cytosol of macrophages via an unique type III secretion system that functions intracellularly to translocate proteins across the phagosomal membrane. Cytoplasmic in infected macrophage

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPIC_SALTY (P74863)

Other databases:

- EMBL:   U51927
- EMBL:   AE006468
- RefSeq:   NP_460358.1
- GeneID:   1252911
- GenomeReviews:   AE006468_GR
- KEGG:   stm:STM1393
- HOGENOM:   HBG673238
- OMA:   IQDIRAE
- ProtClustDB:   PRK15368
- BioCyc:   STYP99287:STM1393-MONOMER
- GO:   GO:0005737
- GO:   GO:0009405

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 13975; Mature: 13975

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAY
CCEECCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
VTLLLAAHPDVHDYAIQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHH
HHHHHHCCCCCCCEEEEEEECCCEECCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHH
KLYSGGV
HHHCCCC
>Mature Secondary Structure
MLAVLKGIPLIQDIRAEGNSRSWIMTIDGHPARGEIFSEAFSISLFLNDLESLPKPCLAY
CCEECCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHH
VTLLLAAHPDVHDYAIQLTADGGWLNGYYTTSSSSELIAIEIEKHLALTCILKNVIRNHH
HHHHHHCCCCCCCEEEEEEECCCEECCEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHH
KLYSGGV
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8755556; 11677609