Click here to switch to the map view.

The map label for this gene is astE [H]

Identifier: 29141651

GI number: 29141651

Start: 1255130

End: 1256098

Strand: Direct

Name: astE [H]

Synonym: t1186

Alternate gene names: 29141651

Gene position: 1255130-1256098 (Clockwise)

Preceding gene: 29141650

Following gene: 29141652

Centisome position: 26.19

GC content: 58.31

Gene sequence:

>969_bases
ATGGATAACTTTCTGGCGTTGACGCTAAGCGGCACGACACCACGGGTGACGCAGGGGAAGGGCGCGGGTTTTCGCTGGCG
CTGGTTAGGCCATGGTCTGCTTGAACTCACGCCCGATGCGCCGGTCGACCGCGCGTTAATTCTTTCTGCGGGGATACACG
GTAATGAAACCGCGCCGGTCGAGATGCTGGATAAGCTGCTGTCGGCGCTGTATTCAGGCAGCTTGACGTTAACGTGGCGA
GTGCTGGTGGTACTCGGCAATCCGCAGGCGCTGGCGGCGGGAATACGCTATTGTCACAGTGATATGAACCGTATGTTTGG
CGGGCGTTGGCAGTCCTTTGCCGAAAGCGATGAAACGCGGCGGGCGCGTGAGCTGGAGCTAAGTCTGGAGACCTTCTTTT
CATCTGGCCAGGCGCGGGTACGCTGGCATCTGGATCTGCATACCGCCATTCGTGGCTCGCATCATTTGCGTTTTGGCGTA
TTGCCGCAGCGCGACCGCCCGTGGGAGGCGGATTTTCTGGCGTGGCTGGGCGCGGCAGGACTGGAGGCGTTGGTATTTCA
TCAGGCGCCCGGCGGTACGTTTACGCACTTTAGCTCTGAACATTTCGGCGCGCTTTCCTGTACGCTGGAGTTGGGAAAGG
CGTTGCCGTTTGGGCAAAACGATCTGACGCAGTTCAGCGTAACCTCGCAGGCGTTGTCGGCGTTGCTGAGCGGTGTCGAA
ACGTCAACCTCGTTTTCGCCGCCGCTACGCTATCGGGTGGTGTCGCAAATCACGCGTCACAGCGACAAGTTCGCGCTTTA
TATGGATGCGCAAACGCTGAATTTTACTGCCTTTGCGAAGGGAACGTTGCTGGCCGAGGAGGGGGATAAGCGCGTGACGG
TGACGCATGACGTTGAATATGTTCTCTTTCCTAATTCCTCTGTCGCCTGCGGATTGCGGGCTGGATTAATGCTGGAAAGA
CTGCCCTGA

Upstream 100 bases:

>100_bases
GATCCGTTATTATTGCGAGAAGGCCGGGAGGCGCTGGATGTGTTAACGCGTCTGCTGGATTTGGGGTCGGTTTATCCTTT
CCAGCAAACGGGGGCGGCTG

Downstream 100 bases:

>100_bases
TAAATGTCCCTGTAAGCTCACGTTAGGATTACAGGGATGATTTGGGACACAGTTTCATGCTATTACGGATTATTCCTAGA
GAATTGCTTTATTAATAAGC

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDNFLALTLSGTTPRVTQGKGAGFRWRWLGHGLLELTPDAPVDRALILSAGIHGNETAPVEMLDKLLSALYSGSLTLTWR
VLVVLGNPQALAAGIRYCHSDMNRMFGGRWQSFAESDETRRARELELSLETFFSSGQARVRWHLDLHTAIRGSHHLRFGV
LPQRDRPWEADFLAWLGAAGLEALVFHQAPGGTFTHFSSEHFGALSCTLELGKALPFGQNDLTQFSVTSQALSALLSGVE
TSTSFSPPLRYRVVSQITRHSDKFALYMDAQTLNFTAFAKGTLLAEEGDKRVTVTHDVEYVLFPNSSVACGLRAGLMLER
LP

Sequences:

>Translated_322_residues
MDNFLALTLSGTTPRVTQGKGAGFRWRWLGHGLLELTPDAPVDRALILSAGIHGNETAPVEMLDKLLSALYSGSLTLTWR
VLVVLGNPQALAAGIRYCHSDMNRMFGGRWQSFAESDETRRARELELSLETFFSSGQARVRWHLDLHTAIRGSHHLRFGV
LPQRDRPWEADFLAWLGAAGLEALVFHQAPGGTFTHFSSEHFGALSCTLELGKALPFGQNDLTQFSVTSQALSALLSGVE
TSTSFSPPLRYRVVSQITRHSDKFALYMDAQTLNFTAFAKGTLLAEEGDKRVTVTHDVEYVLFPNSSVACGLRAGLMLER
LP
>Mature_322_residues
MDNFLALTLSGTTPRVTQGKGAGFRWRWLGHGLLELTPDAPVDRALILSAGIHGNETAPVEMLDKLLSALYSGSLTLTWR
VLVVLGNPQALAAGIRYCHSDMNRMFGGRWQSFAESDETRRARELELSLETFFSSGQARVRWHLDLHTAIRGSHHLRFGV
LPQRDRPWEADFLAWLGAAGLEALVFHQAPGGTFTHFSSEHFGALSCTLELGKALPFGQNDLTQFSVTSQALSALLSGVE
TSTSFSPPLRYRVVSQITRHSDKFALYMDAQTLNFTAFAKGTLLAEEGDKRVTVTHDVEYVLFPNSSVACGLRAGLMLER
LP

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate [H]

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788040, Length=321, Percent_Identity=70.7165109034268, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007036
- InterPro:   IPR016681 [H]

Pfam domain/function: PF04952 AstE_AspA [H]

EC number: =3.5.1.96 [H]

Molecular weight: Translated: 35475; Mature: 35475

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFLALTLSGTTPRVTQGKGAGFRWRWLGHGLLELTPDAPVDRALILSAGIHGNETAPV
CCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCH
EMLDKLLSALYSGSLTLTWRVLVVLGNPQALAAGIRYCHSDMNRMFGGRWQSFAESDETR
HHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHH
RARELELSLETFFSSGQARVRWHLDLHTAIRGSHHLRFGVLPQRDRPWEADFLAWLGAAG
HHHHHHHHHHHHHCCCCEEEEEEEEEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
LEALVFHQAPGGTFTHFSSEHFGALSCTLELGKALPFGQNDLTQFSVTSQALSALLSGVE
CEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHCCC
TSTSFSPPLRYRVVSQITRHSDKFALYMDAQTLNFTAFAKGTLLAEEGDKRVTVTHDVEY
CCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCEEEECCCCCEEEEEECEEE
VLFPNSSVACGLRAGLMLERLP
EEECCCCEECHHHHCCHHHCCC
>Mature Secondary Structure
MDNFLALTLSGTTPRVTQGKGAGFRWRWLGHGLLELTPDAPVDRALILSAGIHGNETAPV
CCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCH
EMLDKLLSALYSGSLTLTWRVLVVLGNPQALAAGIRYCHSDMNRMFGGRWQSFAESDETR
HHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHH
RARELELSLETFFSSGQARVRWHLDLHTAIRGSHHLRFGVLPQRDRPWEADFLAWLGAAG
HHHHHHHHHHHHHCCCCEEEEEEEEEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHCC
LEALVFHQAPGGTFTHFSSEHFGALSCTLELGKALPFGQNDLTQFSVTSQALSALLSGVE
CEEEEEECCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHCCC
TSTSFSPPLRYRVVSQITRHSDKFALYMDAQTLNFTAFAKGTLLAEEGDKRVTVTHDVEY
CCCCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCEEEECCCCCEEEEEECEEE
VLFPNSSVACGLRAGLMLERLP
EEECCCCEECHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA