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The map label for this gene is manX [H]

Identifier: 29141523

GI number: 29141523

Start: 1130027

End: 1130995

Strand: Reverse

Name: manX [H]

Synonym: t1048

Alternate gene names: 29141523

Gene position: 1130995-1130027 (Counterclockwise)

Preceding gene: 29141525

Following gene: 29141522

Centisome position: 23.6

GC content: 53.04

Gene sequence:

>969_bases
GTGACCATTGCTATTGTTATAGGCACACATGGTTGGGCTGCAGAGCAGTTACTTAAAACAGCCGAAATGCTGTTAGGCGA
GCAGGAAAACGTCGGCTGGATCGATTTCGTTCCAGGCGAAAATGCCGAAACGCTGATCGAAAAGTACAACGCTCAGTTGG
CTAAACTCGATACCAGTAAAGGCGTGCTATTTCTCGTTGATACATGGGGAGGCAGTCCGTTTAATGCTGCCAGCCGCATT
GTCGTCGATAAAGAGCGCTATGAAGTTATTGCCGGCGTCAACATCCCGATGCTGGTCGAAACGTTTATGGCTCGTGACGA
CGATCCGAGCTTTGACGAGCTGGTCGCACTGGCGGTAGAAACCGGTCGTGAAGGCGTAAAAGCGCTGAAAGCGAAACCGG
TAGAAAAAGCCGCGCCAGCGCCGGTCGCCGCCGCGCCAAAAGCGGCCACGCCGGCAAAACCGATGGGCCCGAATGATTAT
ATGGTTATCGGGCTTGCCCGTATTGATGACCGCCTCATTCACGGTCAGGTCGCTACCCGCTGGACCAAAGAGACCAATGT
CAGCCGTATTATTGTTGTGAGCGATGAAGTCGCGGCGGATACCGTACGTAAAACGCTGCTGACGCAAGTTGCGCCGCCGG
GCGTTACGGCGCATGTGGTTGACGTCGCGAAGATGATTCGCGTCTACAATAACCCGAAATACGCCGGCGAACGCGTGATG
CTTCTGTTTACCAATCCTACCGACGTCGAGCGCATCGTTGAGGGCGGCGTGAAAGTCACCTCCGTGAACATTGGCGGTAT
GGCCTATCGTCAAGGTAAAACGCAGGTGAACAACGCCGTTTCCGTCGATGAAAAGGATATCGAAGCCTTTAAAAAGCTCA
ACGAGCGCGGCATCGAGCTTGAGGTGCGTAAAGTTTCCACCGATCCGAAACTGAAAATGATGGATTTAATTGCCAAAGTG
GCGAAATAA

Upstream 100 bases:

>100_bases
TTTCGTTGTAACCCTCATCTGAATCGATTCGCTTGCGGACGGCGATTCAAAAAATACATCTGTCAAGTTGATGTGTTGAC
AATAATAAAGGAGGTAGCAA

Downstream 100 bases:

>100_bases
CCGCCGGCATTTAATTAGCTTTCACACTTAAGATAGCAATAGGAGAAGTACAATGGAGATTACCACTCTTCAGATTGTGC
TGGTGTTCATCGTCGCATGT

Product: PTS system mannose-specific transporter IIAB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTSKGVLFLVDTWGGSPFNAASRI
VVDKERYEVIAGVNIPMLVETFMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPVAAAPKAATPAKPMGPNDY
MVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVM
LLFTNPTDVERIVEGGVKVTSVNIGGMAYRQGKTQVNNAVSVDEKDIEAFKKLNERGIELEVRKVSTDPKLKMMDLIAKV
AK

Sequences:

>Translated_322_residues
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTSKGVLFLVDTWGGSPFNAASRI
VVDKERYEVIAGVNIPMLVETFMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPVAAAPKAATPAKPMGPNDY
MVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVM
LLFTNPTDVERIVEGGVKVTSVNIGGMAYRQGKTQVNNAVSVDEKDIEAFKKLNERGIELEVRKVSTDPKLKMMDLIAKV
AK
>Mature_321_residues
TIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTSKGVLFLVDTWGGSPFNAASRIV
VDKERYEVIAGVNIPMLVETFMARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPVAAAPKAATPAKPMGPNDYM
VIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVML
LFTNPTDVERIVEGGVKVTSVNIGGMAYRQGKTQVNNAVSVDEKDIEAFKKLNERGIELEVRKVSTDPKLKMMDLIAKVA
K

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=323, Percent_Identity=94.4272445820433, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=37.5796178343949, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=31.5789473684211, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35018; Mature: 34887

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTSK
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCHHEEEECCC
GVLFLVDTWGGSPFNAASRIVVDKERYEVIAGVNIPMLVETFMARDDDPSFDELVALAVE
CEEEEEECCCCCCCCCCCEEEEEHHHEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVKALKAKPVEKAAPAPVAAAPKAATPAKPMGPNDYMVIGLARIDDRLIHGQVATR
HCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCHHHCCHHHHE
WTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVM
EECCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LLFTNPTDVERIVEGGVKVTSVNIGGMAYRQGKTQVNNAVSVDEKDIEAFKKLNERGIEL
EEEECCCHHHHHHHCCEEEEEEEECCEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCEE
EVRKVSTDPKLKMMDLIAKVAK
EEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAETLIEKYNAQLAKLDTSK
EEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCHHEEEECCC
GVLFLVDTWGGSPFNAASRIVVDKERYEVIAGVNIPMLVETFMARDDDPSFDELVALAVE
CEEEEEECCCCCCCCCCCEEEEEHHHEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVKALKAKPVEKAAPAPVAAAPKAATPAKPMGPNDYMVIGLARIDDRLIHGQVATR
HCHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCHHHCCHHHHE
WTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTAHVVDVAKMIRVYNNPKYAGERVM
EECCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
LLFTNPTDVERIVEGGVKVTSVNIGGMAYRQGKTQVNNAVSVDEKDIEAFKKLNERGIEL
EEEECCCHHHHHHHCCEEEEEEEECCEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCCEE
EVRKVSTDPKLKMMDLIAKVAK
EEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]