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The map label for this gene is fliM [H]

Identifier: 29141388

GI number: 29141388

Start: 998331

End: 999335

Strand: Reverse

Name: fliM [H]

Synonym: t0901

Alternate gene names: 29141388

Gene position: 999335-998331 (Counterclockwise)

Preceding gene: 29141389

Following gene: 29141387

Centisome position: 20.85

GC content: 53.33

Gene sequence:

>1005_bases
ATGGGCGATAGTATTCTTTCTCAGGCTGAAATCGATGCGCTGTTGAACGGCGACAGCGACACCAAAGACGAACCCACTCC
CGGTATCGCAAGCGATAGCGATATTCGCCCTTACGATCCCAATACCCAGCGTCGCGTCGTGCGTGAACGCTTGCAGGCGC
TGGAGATTATTAACGAGCGTTTCGCACGCCAGTTCCGGATGGGATTATTTAACCTGCTGCGCCGCAGCCCGGATATTACC
GTGGGCGCCATACGTATTCAGCCGTATCACGAATTTGCGCGTAACCTGCCAGTACCGACCAACCTGAACCTGATCCACCT
GAAGCCGCTACGCGGCACCGGGCTGGTGGTGTTCTCGCCGAGTCTGGTGTTTATTACCGTCGATAACCTGTTCGGCGGCG
ACGGTCGTTTTCCGACCAAAGTCGAGGGCCGCGAGTTTACCCATACGGAACAGCGGGTCATCAACCGTATGCTGAAACTG
GCGCTGGAAGGCTATAGCGACGCATGGAAAGCCATTAATCCGCTGGAAGTGGAGTATGTGCGTTCCGAAATGCAGGTGAA
ATTTACCAATATCACCACCTCGCCGAACGATATCGTCGTCAATACGCCGTTTCATGTGGAGATCGGCAATCTGACCGGTG
AATTTAACATCTGTCTGCCATTTAGCATGATCGAGCCGCTGCGTGAGCTGCTGGTGAATCCGCCGCTGGAAAACTCGCGC
CATGAAGATCAGAACTGGCGTGATAACCTGGTGCGCCAGGTTCAGCACTCTGAACTGGAGCTGGTGGCCAATTTTGCCGA
TATTCCGTTACGTCTTTCCCAGATTTTGAAGCTGAAACCCGGCGATGTACTGCCGATTGAAAAACCCGACCGCATTATTG
CTCATGTGGACGGCGTTCCGGTACTCACCAGCCAATATGGCACCGTAAACGGACAGTATGCGCTACGCGTAGAACATTTG
ATCAACCCAATTTTGAATTCGCTGAATGAGGAACAGCCCAAATGA

Upstream 100 bases:

>100_bases
GCCGCCATTAAAGAGACGCTGGCCGCGCCGCTCGTTGCCGGACAACCAAAACAGGTCGTCACTGACGTGCTTTATACAGC
TTTTATTCTGCGGTAACGAT

Downstream 100 bases:

>100_bases
GTGACATGAATAATCCGTCCGATGAGAATACTGGCGCATTGGATGATCTGTGGGCTGACGCGTTAAACGAGAAAAAAGCG
ACCACCAATAAAAGCGCCGC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGDSILSQAEIDALLNGDSDTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINERFARQFRMGLFNLLRRSPDIT
VGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFITVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HEDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTVNGQYALRVEHL
INPILNSLNEEQPK

Sequences:

>Translated_334_residues
MGDSILSQAEIDALLNGDSDTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINERFARQFRMGLFNLLRRSPDIT
VGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFITVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HEDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTVNGQYALRVEHL
INPILNSLNEEQPK
>Mature_333_residues
GDSILSQAEIDALLNGDSDTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINERFARQFRMGLFNLLRRSPDITV
GAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSPSLVFITVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLA
LEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSRH
EDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTVNGQYALRVEHLI
NPILNSLNEEQPK

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=334, Percent_Identity=95.2095808383233, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37889; Mature: 37758

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDSILSQAEIDALLNGDSDTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINER
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FARQFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSP
HHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEC
SLVFITVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYV
CEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
RSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HCCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCC
HEDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKPDRIIAHVDGVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VLTSQYGTVNGQYALRVEHLINPILNSLNEEQPK
EEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GDSILSQAEIDALLNGDSDTKDEPTPGIASDSDIRPYDPNTQRRVVRERLQALEIINER
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
FARQFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNLNLIHLKPLRGTGLVVFSP
HHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEC
SLVFITVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKLALEGYSDAWKAINPLEVEYV
CEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHH
RSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNICLPFSMIEPLRELLVNPPLENSR
HCCCEEEEEEEECCCCCEEEECCEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCCC
HEDQNWRDNLVRQVQHSELELVANFADIPLRLSQILKLKPGDVLPIEKPDRIIAHVDGVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
VLTSQYGTVNGQYALRVEHLINPILNSLNEEQPK
EEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]