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The map label for this gene is phsB

Identifier: 29141309

GI number: 29141309

Start: 908496

End: 909074

Strand: Direct

Name: phsB

Synonym: t0810

Alternate gene names: 29141309

Gene position: 908496-909074 (Clockwise)

Preceding gene: 29141308

Following gene: 29141310

Centisome position: 18.96

GC content: 54.23

Gene sequence:

>579_bases
ATGAATCATTTAACGAATCAGTACGTCATGCTGCATGATGAAAAACGTTGTATCGGCTGCCAGGCGTGTACCGTTGCCTG
CAAAGTGCTTAATGACGTACCGGAGGGATTTAGCCGCGTACAGGTACAAATTCGCGCCCCTGAACAGGCATCCAACGCAC
TAACCCATTTTCAATTTGTCCGCGTCTCCTGTCAGCACTGTGAAAATGCGCCATGTGTCAGCGTTTGTCCTACCGGAGCG
TCATATCGTGATGAAAACGGGATCGTGCAGGTGGATAAATCGCGCTGTATTGGCTGCGATTATTGTGTTGCCGCGTGTCC
TTTCCATGTGCGCTATCTGAATCCGCAAACCGGCGTCGCCGACAAGTGTAACTTCTGTGCCGACACGCGGTTGGCGGCTG
GCCAGTCTCCGGCGTGCGTATCCGTTTGCCCAACGGACGCGCTGAAATTCGGCAGACTGGATGAGAGCGAGATCCAGCGC
TGGGTCGGTCAGAAAGAGGTCTATCGCCAGCAGGAAGCGCGTAGCGGCGCGGTCAGTCTGTACCGTCGTAAAGAAGTCCA
TCAGGAGGGTAAAGCATGA

Upstream 100 bases:

>100_bases
ACTCTGGCAATTTACTGCCGCACGTGACGAGTCCGGTATCCGGTACGGTAGTGCATACCGCAGGCGTGACGCTGAGCCGG
GCATGAGGGAGGAGGGAACC

Downstream 100 bases:

>100_bases
ATACTATCTGGGGAGCGGAACTACATTATGCGCCAGATTATTGGCCGCTGTGGTTAATTTACGCAGGCGTCGTGGTGCTG
CTCATGCTTGTTGGGCTGGT

Product: thiosulfate reductase electron transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNHLTNQYVMLHDEKRCIGCQACTVACKVLNDVPEGFSRVQVQIRAPEQASNALTHFQFVRVSCQHCENAPCVSVCPTGA
SYRDENGIVQVDKSRCIGCDYCVAACPFHVRYLNPQTGVADKCNFCADTRLAAGQSPACVSVCPTDALKFGRLDESEIQR
WVGQKEVYRQQEARSGAVSLYRRKEVHQEGKA

Sequences:

>Translated_192_residues
MNHLTNQYVMLHDEKRCIGCQACTVACKVLNDVPEGFSRVQVQIRAPEQASNALTHFQFVRVSCQHCENAPCVSVCPTGA
SYRDENGIVQVDKSRCIGCDYCVAACPFHVRYLNPQTGVADKCNFCADTRLAAGQSPACVSVCPTDALKFGRLDESEIQR
WVGQKEVYRQQEARSGAVSLYRRKEVHQEGKA
>Mature_192_residues
MNHLTNQYVMLHDEKRCIGCQACTVACKVLNDVPEGFSRVQVQIRAPEQASNALTHFQFVRVSCQHCENAPCVSVCPTGA
SYRDENGIVQVDKSRCIGCDYCVAACPFHVRYLNPQTGVADKCNFCADTRLAAGQSPACVSVCPTDALKFGRLDESEIQR
WVGQKEVYRQQEARSGAVSLYRRKEVHQEGKA

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential)

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=54.6961325966851, Blast_Score=206, Evalue=9e-55,
Organism=Escherichia coli, GI2367345, Length=179, Percent_Identity=52.5139664804469, Blast_Score=188, Evalue=2e-49,
Organism=Escherichia coli, GI1787122, Length=158, Percent_Identity=41.1392405063291, Blast_Score=119, Evalue=9e-29,
Organism=Escherichia coli, GI1787872, Length=158, Percent_Identity=39.2405063291139, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787749, Length=160, Percent_Identity=38.125, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1790326, Length=156, Percent_Identity=38.4615384615385, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789370, Length=203, Percent_Identity=33.0049261083744, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI87082180, Length=169, Percent_Identity=36.6863905325444, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI87082179, Length=165, Percent_Identity=34.5454545454545, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788811, Length=155, Percent_Identity=36.7741935483871, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787478, Length=123, Percent_Identity=35.7723577235772, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787963, Length=155, Percent_Identity=29.0322580645161, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367245, Length=166, Percent_Identity=34.9397590361446, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787740, Length=90, Percent_Identity=38.8888888888889, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1789067, Length=179, Percent_Identity=32.4022346368715, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1789079, Length=223, Percent_Identity=26.9058295964126, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHSB_SALTI (P0A1I2)

Other databases:

- EMBL:   AL627273
- EMBL:   AE014613
- RefSeq:   NP_456612.1
- RefSeq:   NP_804651.1
- ProteinModelPortal:   P0A1I2
- SMR:   P0A1I2
- PRIDE:   P0A1I2
- GeneID:   1070081
- GeneID:   1248607
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0810
- KEGG:   sty:STY2270
- HOGENOM:   HBG726839
- OMA:   EIHQEGK
- ProtClustDB:   CLSK894263
- BioCyc:   SENT209261:T0810-MONOMER
- BioCyc:   SENT220341:STY2270-MONOMER
- GO:   GO:0006810
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900

Pfam domain/function: PF00037 Fer4

EC number: NA

Molecular weight: Translated: 21320; Mature: 21320

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
8.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLTNQYVMLHDEKRCIGCQACTVACKVLNDVPEGFSRVQVQIRAPEQASNALTHFQFV
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHH
RVSCQHCENAPCVSVCPTGASYRDENGIVQVDKSRCIGCDYCVAACPFHVRYLNPQTGVA
HHHHHHCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCEEEEEECCCCCCC
DKCNFCADTRLAAGQSPACVSVCPTDALKFGRLDESEIQRWVGQKEVYRQQEARSGAVSL
CCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHH
YRRKEVHQEGKA
HHHHHHHHCCCC
>Mature Secondary Structure
MNHLTNQYVMLHDEKRCIGCQACTVACKVLNDVPEGFSRVQVQIRAPEQASNALTHFQFV
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHH
RVSCQHCENAPCVSVCPTGASYRDENGIVQVDKSRCIGCDYCVAACPFHVRYLNPQTGVA
HHHHHHCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCEEEEEECCCCCCC
DKCNFCADTRLAAGQSPACVSVCPTDALKFGRLDESEIQRWVGQKEVYRQQEARSGAVSL
CCCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHH
YRRKEVHQEGKA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504