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The map label for this gene is phsA [H]

Identifier: 29141308

GI number: 29141308

Start: 906205

End: 908481

Strand: Direct

Name: phsA [H]

Synonym: t0809

Alternate gene names: 29141308

Gene position: 906205-908481 (Clockwise)

Preceding gene: 29141306

Following gene: 29141309

Centisome position: 18.91

GC content: 55.12

Gene sequence:

>2277_bases
ATGAGCATTAGTCGTCGTTCTTTTTTGCAGGGGGTAGGCATAGGCTGCTCTGCCTGCGCGCTGGGCGCTTTTCCGCCCGG
GGCGCTGGCGCGCAACCCGATTGCCGGCATTAACGGTAAAACCACGCTAACGCCAAGTTTGTGCGAAATGTGTTCCTTTC
GTTGCCCTATTCAGGCGCAGGTAGTCAATAACAAGACCGTTTTTATCCAGGGCAATCCTTCTGCGCCGCAGCAGGGGACG
CGCATATGCGCCAGGGGCGGGAGCGGCGTCAGCCTGGTCAATGACCCGCAACGGATTGTAAAACCTATGAAACGCACCGG
GCCACGCGGTGACGGTGAATGGCAGGTGATTAGCTGGCAACAGGCTTACCAGGAAATCGCGGCGAAAATGAATGCCATCA
AAGCGCAGCATGGCCCCGAGAGCGTGGCTTTCTCTTCCAAATCGGGCTCGCTCTCCAGCCATCTTTTCCATCTGGCTACG
GCCTTTGGTTCGCCTAATACCTTTACTCACGCCTCAACATGCCCTGCCGGGAAAGCCATTGCGGCAAAAGTGATGATGGG
CGGCGATCTGGCGATGGATATCGCTAACACGCGCTATCTGGTTTCGTTTGGCCACAATTTGTATGAAGGGATTGAAGTTG
CCGATACCCATGAGTTAATGACCGCGCAGGAGAAGGGGGCCAAAATGGTGAGCTTCGATCCGCGTTTGTCGATATTTTCC
AGCAAGGCGGATGAGTGGCACGCTATTCGTCCCGGAGGGGATTTAGCGGTTCTGCTGGCGATGTGCCACGTCATGATTGA
TGAACAACTCTACGATGCGTCTTTTGTTGAGCGTTATACCAGCGGATTTGAACAGTTAGCACAGGCGGTAAAAGAGACGA
CGTCGGAATGGGCCGCCGCGCAGGCCGATGTCCCAGCCGACGTCATTGTCCGGGTGACACGCGAACTGGCCGCCTGTGCG
CCTCACGCTATTGTCAGTCCTGGTCATCGCGCGACGTTCTCGCAGGAAGAGATCGATATGCGGCGTATGATTTTTACGCT
TAATGTGCTGCTCGGTAATATTGAGCGCGAAGGCGGGCTATATCAGAAAAAAAACGCGTCGGTTTACAACAAACTGGCCG
GAGAAAAGGTCGCGCCAACGCTGGCGAAACTCAACATTAAAAATATGCCGAAACCGACGGCGCAACGAATCGATCTGGTC
GCACCTCAGTTTAAATATATCGCCGCTGGCGGCGGCGTGGTGCAAAGCATTATTGACGCGGCGTTAACCCAGAAGCCTTA
CCCGATAAAGGCGTGGATTATGTCGCGGCATAATCCTTTCCAGACCGTCACCTGTCGTTCGGACCTGGTAAAAACCGTTG
AGCAACTGGATCTGGTGGTCAGCTGCGATGTCTATTTGAGCGAGAGCGCGGCATATGCCGACTATCTGCTGCCGGAATGC
ACCTATCTCGAACGGGACGAAGAGGTATCCGATATGTCGGGTCTGCACCCGGCTTACGCTCTGCGCCAACAGGTCGTAGA
GCCGATTGGCGAGGCGCGTCCGAGTTGGCAAATCTGGAAAGAACTTGGCGAGCAGTTGGGATTAGGGCAGTACTATCCGT
GGCAGGATATGCAGACGCGCCAACTCTATCAGTTGAACGGCGACCATGCCTTAGCGAAGGAACTGCGACAGAAAGGGTAT
CTCGAATGGGGCGTTCCGCTGCTATTACGCGAACCAGAATCCGTTCGTCAGTTTACGGCGCGTTACCCCGGCGCTATCGC
GACGGACAGTGACAACACCTATGGCGAACAGCTTCGCTTCAAATCGCCCTCCGGCAAAATCGAACTTTATTCCGCAACCC
TGGAGGAATTGCTCCCTGGCTACGGCGTTCCGCGCGTTCGTGACTTTGCGCTGAAAAAAGAGAATGAGCTTTACTTCATT
CAAGGCAAGGTGGCCGTGCATACCAATGGCGCGACGCAGTACGTACCTTTACTCAGCGAGCTAATGTGGGATAACGCGGT
CTGGGTCCATCCGCAAACAGCGCAAGAAAAAGGCATTAAGACCGGCGATGAGATCTGGCTGGAAAATGCCACGGGTAAAG
AGAAAGGTAAGGCGCTGGTGACGCCCGGTATTCGCCCGGATACGCTTTTTGTCTATATGGGATTTGGCGCTAAAGCTGGG
GCTAAAACGGCGGCGACGACACACGGTATCCACTCTGGCAATTTACTGCCGCACGTGACGAGTCCGGTATCCGGTACGGT
AGTGCATACCGCAGGCGTGACGCTGAGCCGGGCATGA

Upstream 100 bases:

>100_bases
CATGCTCATATTAACAACTATTTCAAATTGTGATTGAAGCCGACATTTCATAATACAAATTAAATATAATCTTCAGCTAT
ATCTAATAACAGGAGGTTAT

Downstream 100 bases:

>100_bases
GGGAGGAGGGAACCATGAATCATTTAACGAATCAGTACGTCATGCTGCATGATGAAAAACGTTGTATCGGCTGCCAGGCG
TGTACCGTTGCCTGCAAAGT

Product: thiosulfate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGT
RICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLAT
AFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTSEWAAAQADVPADVIVRVTRELAACA
PHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLV
APQFKYIAAGGGVVQSIIDAALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGY
LEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
AKTAATTHGIHSGNLLPHVTSPVSGTVVHTAGVTLSRA

Sequences:

>Translated_758_residues
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGT
RICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLAT
AFGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTSEWAAAQADVPADVIVRVTRELAACA
PHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLV
APQFKYIAAGGGVVQSIIDAALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGY
LEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFI
QGKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
AKTAATTHGIHSGNLLPHVTSPVSGTVVHTAGVTLSRA
>Mature_757_residues
SISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQVVNNKTVFIQGNPSAPQQGTR
ICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQQAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATA
FGSPNTFTHASTCPAGKAIAAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFSS
KADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTSEWAAAQADVPADVIVRVTRELAACAP
HAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGLYQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVA
PQFKYIAAGGGVVQSIIDAALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPECT
YLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTRQLYQLNGDHALAKELRQKGYL
EWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRFKSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQ
GKVAVHTNGATQYVPLLSELMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAGA
KTAATTHGIHSGNLLPHVTSPVSGTVVHTAGVTLSRA

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=835, Percent_Identity=26.3473053892216, Blast_Score=195, Evalue=1e-50,
Organism=Escherichia coli, GI87081797, Length=791, Percent_Identity=25.4108723135272, Blast_Score=182, Evalue=8e-47,
Organism=Escherichia coli, GI171474008, Length=828, Percent_Identity=25, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI3868721, Length=726, Percent_Identity=24.3801652892562, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1787231, Length=717, Percent_Identity=21.8967921896792, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI87081994, Length=795, Percent_Identity=22.1383647798742, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788534, Length=826, Percent_Identity=20.4600484261501, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 82758; Mature: 82627

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQ
CCCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQ
EECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHH
QAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAI
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
HHHHEECCCEEEECCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHC
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTSEWAAA
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGL
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDA
CCCCCCHHHHHHCCCHHCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
ALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
HHCCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTR
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCH
QLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRF
HHEEECCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCCCCEEE
KSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSE
ECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
HHHCCEEEECCCHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCC
AKTAATTHGIHSGNLLPHVTSPVSGTVVHTAGVTLSRA
CCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEECC
>Mature Secondary Structure 
SISRRSFLQGVGIGCSACALGAFPPGALARNPIAGINGKTTLTPSLCEMCSFRCPIQAQ
CCCHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHCCCCCEEEE
VVNNKTVFIQGNPSAPQQGTRICARGGSGVSLVNDPQRIVKPMKRTGPRGDGEWQVISWQ
EECCEEEEEECCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHH
QAYQEIAAKMNAIKAQHGPESVAFSSKSGSLSSHLFHLATAFGSPNTFTHASTCPAGKAI
HHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
AAKVMMGGDLAMDIANTRYLVSFGHNLYEGIEVADTHELMTAQEKGAKMVSFDPRLSIFS
HHHHEECCCEEEECCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHCCEEEECCCCHHHHC
SKADEWHAIRPGGDLAVLLAMCHVMIDEQLYDASFVERYTSGFEQLAQAVKETTSEWAAA
CCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QADVPADVIVRVTRELAACAPHAIVSPGHRATFSQEEIDMRRMIFTLNVLLGNIEREGGL
CCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
YQKKNASVYNKLAGEKVAPTLAKLNIKNMPKPTAQRIDLVAPQFKYIAAGGGVVQSIIDA
CCCCCCHHHHHHCCCHHCCHHHHCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
ALTQKPYPIKAWIMSRHNPFQTVTCRSDLVKTVEQLDLVVSCDVYLSESAAYADYLLPEC
HHCCCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHCEEEEECEEECCCHHHHHHHCCCC
TYLERDEEVSDMSGLHPAYALRQQVVEPIGEARPSWQIWKELGEQLGLGQYYPWQDMQTR
CCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCH
QLYQLNGDHALAKELRQKGYLEWGVPLLLREPESVRQFTARYPGAIATDSDNTYGEQLRF
HHEEECCCHHHHHHHHHCCCEECCCCEEEECCHHHHHHHHHCCCCEECCCCCCCCCCEEE
KSPSGKIELYSATLEELLPGYGVPRVRDFALKKENELYFIQGKVAVHTNGATQYVPLLSE
ECCCCCEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEEEEEEEEEEECCCHHHHHHHHH
LMWDNAVWVHPQTAQEKGIKTGDEIWLENATGKEKGKALVTPGIRPDTLFVYMGFGAKAG
HHHCCEEEECCCHHHHCCCCCCCCEEEECCCCCCCCCEEECCCCCCCEEEEEECCCCCCC
AKTAATTHGIHSGNLLPHVTSPVSGTVVHTAGVTLSRA
CCCHHHHCCCCCCCCCCCCCCCCCCCEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]