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The map label for this gene is wcaL [H]

Identifier: 29141272

GI number: 29141272

Start: 863277

End: 864497

Strand: Direct

Name: wcaL [H]

Synonym: t0772

Alternate gene names: 29141272

Gene position: 863277-864497 (Clockwise)

Preceding gene: 29141271

Following gene: 29141273

Centisome position: 18.02

GC content: 56.51

Gene sequence:

>1221_bases
ATGAAAGTCAGCTTTTTTCTGCTGAAATTTCCACTCTCATCGGAAACCTTTGTGCTGAATCAGATTACTGCGTTTATTGA
TATGGGCCATGAGGTGGAGATTGTCGCGTTACAAAAAGGCGATACCCAACATACTCACGCCGCCTGGGAGAAGTATGGCC
TGGCGGCGAAAACCCGCTGGTTACAGGATGAGCCCCAGGGACGGCTGGCGAAGCTGCGCTACCGGGCATGTAAAACGCTG
CCGGGGCTGCATCGGGCGGCGACCTGGAAAGCGCTCAATTTTACCCGCTACGGCGATGAATCACGCAATTTGATCCTTTC
CGCGATTTGCGCGCAGGCGGGCCAACCTTTTGTGGCGGATGTGTTCATCGCGCACTTTGGTCCGGCGGGCGTGACGGCGG
CCAAACTGCGCGAACTGGGCGTGCTTCGCGGCAAAATCGAGACTATTTTCCACGGAATTGATATTTCCAGTCGCGAGGTG
CTCAGTCATTACACGCCGGAGTATCAGCAGTTGTTTCGTCGTGGCGATCTGATGCTGCCCATCAGCGATCTGTGGGCCGG
TCGCCTGAAAAGTATGGGCTGTCCGCCGGAAAAGATTGCCGTTTCGCGCATGGGCGTCGACATGACGCGTTTTTCCCATC
GTCCGGTGAAAGCGCCAGGGATGCCGCTGGAGATGATTTCCGTCGCGCGCCTGACCGAGAAAAAAGGCCTGCATGTGGCG
ATTGAAGCCTGTCGGCAACTGAAAGCGCAGGGCGTGGCGTTTCGCTACCGCATTCTGGGGATTGGCCCGTGGGAACGTCG
GCTGCGCACGCTCATTGAGCAGTATCAGTTAGAGGATGTCATTGAGATGCCGGGTTTTAAACCGAGCCATGAAGTGAAGG
CGATGCTGGATGACGCCGATGTTTTTTTGCTGCCGTCGATTACCGGTACGGATGGCGATATGGAAGGTATTCCGGTAGCG
CTGATGGAGACGATGGCGGTGGGGATTCCCGTGGTGTCTACCGTGCATAGCGGTATTCCGGAACTGGTGGAGGCCGGCAA
ATCCGGCTGGCTGGTACCGGAAAACGATGCGCAGGCGCTGGCGGCCCGACTCGCTGAGTTCAGCCGGATTGACCACGATA
CGCTGGGGTCGGTGATCATGCGCGCTCGTGAAAAAGTGGCGCAAGATTTTAATCAGCAGGCGATTAATCGCCAGTTAGCC
AGCCTGCTACAAACCATATAA

Upstream 100 bases:

>100_bases
TGCCTGCGCTAAAGGCAAAAGTGGCTAACGCCGTCACCGAGGAGCGGGCGAAAGGGTTCACGATGATCAAATCTGCTCTC
GACAGAATTCGGGAGGCGCA

Downstream 100 bases:

>100_bases
ACGAGGTGGTATGCCCGCGACTAAATTCTCCCGACGTACCCTCCTGACGGCAGGTTCCGCGCTTGCTGTTCTTCCTTTTC
TGCGCGCCTTGCCGGTACAG

Product: glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAATWKALNFTRYGDESRNLILSAICAQAGQPFVADVFIAHFGPAGVTAAKLRELGVLRGKIETIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMETMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLGSVIMRAREKVAQDFNQQAINRQLA
SLLQTI

Sequences:

>Translated_406_residues
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAATWKALNFTRYGDESRNLILSAICAQAGQPFVADVFIAHFGPAGVTAAKLRELGVLRGKIETIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMETMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLGSVIMRAREKVAQDFNQQAINRQLA
SLLQTI
>Mature_406_residues
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRWLQDEPQGRLAKLRYRACKTL
PGLHRAATWKALNFTRYGDESRNLILSAICAQAGQPFVADVFIAHFGPAGVTAAKLRELGVLRGKIETIFHGIDISSREV
LSHYTPEYQQLFRRGDLMLPISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDADVFLLPSITGTDGDMEGIPVA
LMETMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQALAARLAEFSRIDHDTLGSVIMRAREKVAQDFNQQAINRQLA
SLLQTI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=406, Percent_Identity=85.2216748768473, Blast_Score=705, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 45178; Mature: 45178

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRW
CCEEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHC
LQDEPQGRLAKLRYRACKTLPGLHRAATWKALNFTRYGDESRNLILSAICAQAGQPFVAD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEEEHCCCCCHHHHHHHHHHHHCCCCHHHH
VFIAHFGPAGVTAAKLRELGVLRGKIETIFHGIDISSREVLSHYTPEYQQLFRRGDLMLP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSITGTDGDMEGIPVALMETMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AARLAEFSRIDHDTLGSVIMRAREKVAQDFNQQAINRQLASLLQTI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKVSFFLLKFPLSSETFVLNQITAFIDMGHEVEIVALQKGDTQHTHAAWEKYGLAAKTRW
CCEEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHC
LQDEPQGRLAKLRYRACKTLPGLHRAATWKALNFTRYGDESRNLILSAICAQAGQPFVAD
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHEEEHCCCCCHHHHHHHHHHHHCCCCHHHH
VFIAHFGPAGVTAAKLRELGVLRGKIETIFHGIDISSREVLSHYTPEYQQLFRRGDLMLP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCEEE
ISDLWAGRLKSMGCPPEKIAVSRMGVDMTRFSHRPVKAPGMPLEMISVARLTEKKGLHVA
HHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
IEACRQLKAQGVAFRYRILGIGPWERRLRTLIEQYQLEDVIEMPGFKPSHEVKAMLDDAD
HHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VFLLPSITGTDGDMEGIPVALMETMAVGIPVVSTVHSGIPELVEAGKSGWLVPENDAQAL
EEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHH
AARLAEFSRIDHDTLGSVIMRAREKVAQDFNQQAINRQLASLLQTI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]