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The map label for this gene is gltX [H]

Identifier: 29140965

GI number: 29140965

Start: 512524

End: 513939

Strand: Direct

Name: gltX [H]

Synonym: t0442

Alternate gene names: 29140965

Gene position: 512524-513939 (Clockwise)

Preceding gene: 29140964

Following gene: 29140968

Centisome position: 10.7

GC content: 55.23

Gene sequence:

>1416_bases
ATGAAAATCAAAACTCGCTTCGCGCCTAGCCCGACAGGTTATCTGCACGTCGGCGGCGCGCGTACTGCTCTTTATTCCTG
GCTTTTCGCACGTCACCACGGCGGTGAATTCGTGCTGCGTATTGAAGACACCGATCTTGAGCGTTCCACGCCGGAAGCCA
TTGAAGCCATCATGGATGGTATGAACTGGCTGAACCTGGAATGGGACGAAGGCCCATATTTCCAGACCAAACGTTTTGAT
CGCTATAACGCGGTGATTGACGAGATGCTGGAGGCGGGAACAGCCTATAAATGCTACTGCTCTAAAGAGCGTCTGGAGCA
ACTGCGTGAAGATCAGATGGCGAAAGGCGAGAAGCCGCGTTATGACGGGCGTTGCCGTCACAGCCACGAACATCATGCCG
ACGATGAGCCATGCGTGGTGCGCTTTGCCAACCCGCAGGACGGTTCCGTTATTTTTGATGATCAGATCCGTGGCCCGATC
GAATTCAGCAATCAGGAACTGGACGATCTGATCATTCGTCGTACCGACGGTTCCCCAACCTACAACTTCTGTGTGGTGGT
GGACGACTGGGATATGGAAATTACCCATGTGATTCGTGGCGAAGACCATATCAACAATACTCCGCGCCAGATTAACATTC
TGAAAGCGTTAAACGCGCCGGTGCCGATGTACGCGCATGTGTCGATGATTAACGGCGACGACGGTAAAAAGCTGTCTAAA
CGTCACGGTGCGGTGAGCGTGATGCAGTATCGCGATGACGGTTATCTGCCGGAAGCGTTGCTGAACTATCTGGTGCGTCT
GGGCTGGTCCAGCGGCGATCAGGAAATCTTCACCCGCGAAGAGATGATCAAGCTGTTCTCCCTCGGTGCGGTGAGCAAGT
CTGCGAGCGCGTTCAACACCGATAAACTGCTGTGGCTGAACCACCACTATATCAATACGCTGGCGCCGGAATATGTGGCG
ACGCATTTGCAGTGGCATATCGAGCAGGAAAATATCGACACCCGTAACGGCCCGCAGCTTGCTGAGCTGGTGAAGTTGCT
GGGCGAACGCTGCAAGACTCTGAAAGAGATGGCGCAGAGCTGCCGCTACTTCTATGAAGACTTCAGCGAGTTTGACGCCG
ATGCGGCGAAAAAACACCTCCGCCCGGTGGCGCGTCAGCCGCTGGAAGTGGTGCGCGACAAGCTGTCGGCGATTACGGAC
TGGTCAGCAGAAAACGTCCACCACGCGATTCAGGCCACGGCGGATGAGCTGGAAGTGGGGATGGGTAAAGTAGGGATGCC
GCTGCGTGTGGCTGTGACCGGTGCCGGGCAGTCGCCTGCGCTGGATGTCACCGTACATGCGATTGGTAAAACCCGCAGCA
TTGAGCGTATCAACAAAGCGCTGGGCTTTATCGCCGAGCGTGAAAGTCAGCAGTAA

Upstream 100 bases:

>100_bases
ATCAACATTGAGGAATCAAGCCACAGTGATAGAATACAAGGCTATTCATCTTTCCAGGATTTGCCGGTTGTCGGCATCTT
TATAAACGTAAGGCCATTTC

Downstream 100 bases:

>100_bases
CGCCAGCTGAAACAGAATAAACGGCAGGGCAGACGCCTGCCGTTTATTATTTGTTAGTCCGCCTCATTGATACCTAAACG
CGTCAGAAATCCGCGAATAC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLNLEWDEGPYFQTKRFD
RYNAVIDEMLEAGTAYKCYCSKERLEQLREDQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQDGSVIFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALNAPVPMYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSSGDQEIFTREEMIKLFSLGAVSKSASAFNTDKLLWLNHHYINTLAPEYVA
THLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQSCRYFYEDFSEFDADAAKKHLRPVARQPLEVVRDKLSAITD
WSAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALGFIAERESQQ

Sequences:

>Translated_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLNLEWDEGPYFQTKRFD
RYNAVIDEMLEAGTAYKCYCSKERLEQLREDQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQDGSVIFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALNAPVPMYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSSGDQEIFTREEMIKLFSLGAVSKSASAFNTDKLLWLNHHYINTLAPEYVA
THLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQSCRYFYEDFSEFDADAAKKHLRPVARQPLEVVRDKLSAITD
WSAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALGFIAERESQQ
>Mature_471_residues
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHHGGEFVLRIEDTDLERSTPEAIEAIMDGMNWLNLEWDEGPYFQTKRFD
RYNAVIDEMLEAGTAYKCYCSKERLEQLREDQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQDGSVIFDDQIRGPI
EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALNAPVPMYAHVSMINGDDGKKLSK
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSSGDQEIFTREEMIKLFSLGAVSKSASAFNTDKLLWLNHHYINTLAPEYVA
THLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQSCRYFYEDFSEFDADAAKKHLRPVARQPLEVVRDKLSAITD
WSAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALGFIAERESQQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=31.1740890688259, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=95.1167728237792, Blast_Score=949, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=244, Percent_Identity=36.4754098360656, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=28.6885245901639, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=30.1229508196721, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=496, Percent_Identity=31.8548387096774, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=32.3076923076923, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=25.3275109170306, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24665321, Length=490, Percent_Identity=31.4285714285714, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53634; Mature: 53634

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLNLEWDEGPYFQTKRFDRYNAVIDEMLEAGTAYKCYCSKERLEQLREDQMAKGEKPR
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQDGSVIFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALNAPVPMYAHVSMINGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCCCEEHHHHCCCCCCCEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSSGDQEIFTREEMIKLFSLGAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DKLLWLNHHYINTLAPEYVATHLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQS
CEEEEEECHHHHHCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFSEFDADAAKKHLRPVARQPLEVVRDKLSAITDWSAENVHHAIQATADELEVG
HHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALGFIAERESQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKTRFAPSPTGYLHVGGARTALYSWLFARHHGGEFVLRIEDTDLERSTPEAIEAIMDG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCC
MNWLNLEWDEGPYFQTKRFDRYNAVIDEMLEAGTAYKCYCSKERLEQLREDQMAKGEKPR
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCC
YDGRCRHSHEHHADDEPCVVRFANPQDGSVIFDDQIRGPIEFSNQELDDLIIRRTDGSPT
CCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCCCCCCHHEEEECCCCCC
YNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALNAPVPMYAHVSMINGDDGKKLSK
EEEEEEEECCCCEEEEEECCCHHCCCCCCCEEHHHHCCCCCCCEEEEEEEECCCCHHHHH
RHGAVSVMQYRDDGYLPEALLNYLVRLGWSSGDQEIFTREEMIKLFSLGAVSKSASAFNT
HCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DKLLWLNHHYINTLAPEYVATHLQWHIEQENIDTRNGPQLAELVKLLGERCKTLKEMAQS
CEEEEEECHHHHHCCHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CRYFYEDFSEFDADAAKKHLRPVARQPLEVVRDKLSAITDWSAENVHHAIQATADELEVG
HHHHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
MGKVGMPLRVAVTGAGQSPALDVTVHAIGKTRSIERINKALGFIAERESQQ
CCCCCCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA