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The map label for this gene is amiA [H]

Identifier: 29140935

GI number: 29140935

Start: 483295

End: 484164

Strand: Reverse

Name: amiA [H]

Synonym: t0408

Alternate gene names: 29140935

Gene position: 484164-483295 (Counterclockwise)

Preceding gene: 29140946

Following gene: 29140934

Centisome position: 10.1

GC content: 50.46

Gene sequence:

>870_bases
ATGAGCACTTTTAAACTCCTAAAAACTCTCACATCGCGCCGCCAGGTACTCAAAACAGGTCTGGCGGCCCTGACGCTGTC
AGGTATGTCGCACGCAATAGCCAAAGAAGAAACGCTAAAAACCAGTAATGGTCACAGCAAACCCAAAACCAAAAAAACCG
GCAGTAAACGGTTGGTGATGCTCGACCCCGGACACGGCGGCATCGATACCGGCGCGATTGGCCGCAACGGTTCTCAGGAA
AAACACGTCGTACTGGCAATTGCCAAAAACGTACGCGCTATTTTGCGAAATCACGGAATCGATGCGCGTTTAACCCGGAC
AGGGGATACCTTTATTCCGCTGTACGATCGGGTGGAAATCGCCCATAAGCACGGCGCGGATCTGTTTATGTCTATTCATG
CCGATGGTTTTACCAATCCGAAAGCGGCGGGCGCTTCCGTTTTTGCGCTTTCCAACCGCGGGGCCAGTAGCGCCATGGCG
AAGTATCTCTCCGAGCGTGAAAACCGCGCGGACGAAGTGGCGGGTAAAAAAGCCACTGACCGGGATCATCTATTACAACA
AGTTTTGTTTGATCTGGTGCAAACCGATACGATTAAAAACAGTCTGACGCTGGGTTCGCATATTCTCAAGAAAATCAAAC
CCATACATAAATTACACAGCCGCACTACCGAACAAGCGGCTTTCGTGGTGCTAAAATCACCGTCAATTCCGTCGGTACTG
GTCGAAACCTCGTTTATTACCAATCCGGAAGAAGAACGCCTGCTGGGCACGACGGCGTTTCGCCAGAAAATCGCTACGGC
GATCGCCAACGGCATCATCAGTTATTTTCACTGGTTTGATAATCAGAAAGCACACACGAAGAAACGGTAA

Upstream 100 bases:

>100_bases
TATTGAAATGAAAAGTAAAACAATTCTCATCACCAACCGTCGTAACGGATTACGCGATACGATATAACATCTTGAACTTA
ATTTTCACAACTCAGGCCGT

Downstream 100 bases:

>100_bases
TCATGAAACCCGATGCACATCACGTAAAACAGTTTCTGCTACGCCTGCAGGATGATATTTGCCAAACGTTATCCGCTGTG
GATGGCGCAAACTTTATCGA

Product: N-acetylmuramoyl-l-alanine amidase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSTFKLLKTLTSRRQVLKTGLAALTLSGMSHAIAKEETLKTSNGHSKPKTKKTGSKRLVMLDPGHGGIDTGAIGRNGSQE
KHVVLAIAKNVRAILRNHGIDARLTRTGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDRDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPIHKLHSRTTEQAAFVVLKSPSIPSVL
VETSFITNPEEERLLGTTAFRQKIATAIANGIISYFHWFDNQKAHTKKR

Sequences:

>Translated_289_residues
MSTFKLLKTLTSRRQVLKTGLAALTLSGMSHAIAKEETLKTSNGHSKPKTKKTGSKRLVMLDPGHGGIDTGAIGRNGSQE
KHVVLAIAKNVRAILRNHGIDARLTRTGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
KYLSERENRADEVAGKKATDRDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPIHKLHSRTTEQAAFVVLKSPSIPSVL
VETSFITNPEEERLLGTTAFRQKIATAIANGIISYFHWFDNQKAHTKKR
>Mature_288_residues
STFKLLKTLTSRRQVLKTGLAALTLSGMSHAIAKEETLKTSNGHSKPKTKKTGSKRLVMLDPGHGGIDTGAIGRNGSQEK
HVVLAIAKNVRAILRNHGIDARLTRTGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAK
YLSERENRADEVAGKKATDRDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPIHKLHSRTTEQAAFVVLKSPSIPSVLV
ETSFITNPEEERLLGTTAFRQKIATAIANGIISYFHWFDNQKAHTKKR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=289, Percent_Identity=92.7335640138408, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI87082163, Length=239, Percent_Identity=48.9539748953975, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790611, Length=244, Percent_Identity=39.344262295082, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31674; Mature: 31543

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKLLKTLTSRRQVLKTGLAALTLSGMSHAIAKEETLKTSNGHSKPKTKKTGSKRLVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
LDPGHGGIDTGAIGRNGSQEKHVVLAIAKNVRAILRNHGIDARLTRTGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCCEECHHHHHHH
AHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPIHKLHSRTTEQAAFVVLKSPSIPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHE
VETSFITNPEEERLLGTTAFRQKIATAIANGIISYFHWFDNQKAHTKKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STFKLLKTLTSRRQVLKTGLAALTLSGMSHAIAKEETLKTSNGHSKPKTKKTGSKRLVM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
LDPGHGGIDTGAIGRNGSQEKHVVLAIAKNVRAILRNHGIDARLTRTGDTFIPLYDRVEI
ECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCCEECHHHHHHH
AHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATD
HHHCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
RDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPIHKLHSRTTEQAAFVVLKSPSIPSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHE
VETSFITNPEEERLLGTTAFRQKIATAIANGIISYFHWFDNQKAHTKKR
EEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]