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The map label for this gene is ispG [H]

Identifier: 29140865

GI number: 29140865

Start: 383225

End: 384343

Strand: Direct

Name: ispG [H]

Synonym: t0333

Alternate gene names: 29140865

Gene position: 383225-384343 (Clockwise)

Preceding gene: 29140864

Following gene: 29140866

Centisome position: 8.0

GC content: 55.05

Gene sequence:

>1119_bases
ATGCATAACCAGGCTCCGATTCAACGTAGAAAATCGACACGTATTTACGTTGGGAATGTGCCGATTGGCGATGGCGCCCC
TATCGCCGTCCAGTCGATGACTAACACGCGTACCACTGACGTGGAAGCGACGGTTAATCAGATCAAAGCGCTGGAGCGCG
TTGGCGCAGATATTGTTCGTGTTTCTGTACCGACCATGGACGCCGCCGAAGCGTTCAAGCTTATCAAACAGCAGGTTAGC
GTCCCGCTGGTTGCCGATATTCACTTCGACTACCGCATTGCGCTGAAAGTAGCGGAATACGGCGTTGACTGCTTGCGTAT
TAACCCCGGCAATATCGGTAACGAAGAACGCATTCGCATGGTGGTGGACTGCGCGCGCGACAAAAACATCCCTATCCGTA
TCGGCGTAAATGCCGGATCGCTGGAAAAAGATCTGCAGGAAAAATACGGAGAGCCGACGCCGCAGGCGCTGCTGGAATCA
GCCATGCGCCATGTCGATCATCTCGATCGTCTCAACTTTGATCAGTTCAAAGTGAGCGTAAAAGCGTCCGATGTATTCCT
TGCCGTTGAGTCTTATCGTTTGTTGGCTAAACAGATCGATCAGCCGCTGCATCTGGGGATCACCGAAGCGGGCGGCGCGC
GTAGCGGGGCGGTTAAGTCGGCGATCGGTTTAGGTCTGCTGTTATCGGAAGGCATCGGCGACACGCTGCGCGTATCGTTG
GCGGCCGACCCGGTGGAAGAGATCAAAGTCGGTTTCGATATCCTGAAGTCGCTGCGTATTCGCGCGCGCGGCATCAATTT
CATCGCCTGCCCGACCTGTTCTCGTCAGGAGTTCGACGTTATCGGCACGGTGAACGCGCTGGAGCAGCGTCTGGAAGATA
TCATCACCCCGATGGACGTCTCGATCATCGGTTGTGTGGTGAACGGGCCGGGCGAAGCGCTGGTATCGACGCTGGGCGTG
ACGGGCGGCAATAAGAAAAGCGGCCTGTATGAAGACGGCGTGCGTAAGGACAGACTCGATAACGACGATATGATCGCGCA
GCTTGAATCCCGTATTCGCGCGAAAGTAAGTCAACTTGATGAAGCGCGTCGGATTGACGTGCTGCAGGTTGAAAAATAA

Upstream 100 bases:

>100_bases
ATCTGAGCCGCTTTATCAGAACTAATCAGGTTGCGCGTCTGACCCTTAATGCCGAACCGACACCGGCGCAGTAACAGACG
GGTAACGCGGGAGATTTTTC

Downstream 100 bases:

>100_bases
GAACGTGATGGGAAGCGGCGCGCTTCCCGTGTATGATTGAACCCGCATGGCTCCCGGTTCGTTCGGGGAAGCGCTGAGGG
TTCATTTTTATATTCAGAAA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGV
TGGNKKSGLYEDGVRKDRLDNDDMIAQLESRIRAKVSQLDEARRIDVLQVEK

Sequences:

>Translated_372_residues
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGV
TGGNKKSGLYEDGVRKDRLDNDDMIAQLESRIRAKVSQLDEARRIDVLQVEK
>Mature_372_residues
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVS
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
AADPVEEIKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGV
TGGNKKSGLYEDGVRKDRLDNDDMIAQLESRIRAKVSQLDEARRIDVLQVEK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=372, Percent_Identity=98.1182795698925, Blast_Score=739, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40627; Mature: 40627

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVR
CCCCCCCHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRM
EECCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEHHCCCEEEEECCCCCCCHHHEEE
VVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSV
EEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
KASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
EECEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNDDMIAQLESRIRAKVSQLD
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EARRIDVLQVEK
HHHCCCEEEECC
>Mature Secondary Structure
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVR
CCCCCCCHHCCCCEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQQVSVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRM
EECCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEHHCCCEEEEECCCCCCCHHHEEE
VVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSV
EEECCCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
KASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSL
EECEEEEEHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNDDMIAQLESRIRAKVSQLD
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EARRIDVLQVEK
HHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA