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The map label for this gene is ybbF [H]

Identifier: 29140821

GI number: 29140821

Start: 324194

End: 325555

Strand: Direct

Name: ybbF [H]

Synonym: t0287

Alternate gene names: 29140821

Gene position: 324194-325555 (Clockwise)

Preceding gene: 29140820

Following gene: 29140822

Centisome position: 6.77

GC content: 56.02

Gene sequence:

>1362_bases
ATGGATAAGACGGCAGCGCTCGCCAGCGACATTTTACGTGGTATCGGCGGAGAGCAAAATATTCTGCGCCTGGAAAACTG
TATGACGCGAGTCAGAGTGGAGGTACAGGACGACAGCCAACTGGATATTCCGCGCTTAAAAGCGTTGCCTGGCGTCAGTG
GATACGTAAAGCAAGGGGAGCAACATCAGCTGATCGTTGGGCCGGGGAAGGCTGCACAGGTGGTTGATGCGATGCGCGTG
CAGATTGCCGCAGGTGGTGTAAAGCCGGACGATGCTATGGCGCGTACCAAATCCGAGGCGAAAGCGAAGTATAAAGCGCC
GATGAGCGATGCGTTGCGTAAGCTGGCGAATGTTTTTATTCCGCTTATTCCCGCCTTTATCGCATCCGGCTTGATTACCG
GCATTATCAATATTCTTAAACGCCCGGATATCGTCGGCGATGTTGCCGTCCACTATCCTAACCTGCTGGGCCTGATGGGG
ATATTTGGTAGCGCGGTGTTCGCTATCATGAACATTCTGGTTGGCGTCAATACCGCGAAAGTCTTTGGCGGATCGCAGGC
GTTAGGCGGCGTGATGGCGGGGATCCTTTCCAGCCCGCAACTGGCGCAGATTACGCTCTTTGGCGAGGCGCTTCAGCCGG
GACGGGGCGGCGTTATCGCCGTCCTGCTGGTGGTGGCGTTAATGTGCTGGATTGAGCGCCAGTTCCGTAAGCTGCTGCCG
GGGTCGCTGGAGTTAATTCTTAACCCTTTATTGACGACTGTTATCACCGGCGCGGTAGCGATTGTCGCTCTGCAACCGCT
TGGCGGCTGGATTTCAGACGCTATCGCTCACGGCGCGTCCTGGGCTATCGATCGCGGCGGTTTTCTGGTGGGCGCGGTGT
TAGCCGGAACCTTCCTGCCGTTGGTACTGATCGGGCTGCATCAGGGGCTGGTGCCTATTCATGTGGAGCTGGTGCAAGCG
CACGGCTATAACGCGCTGTTTCCCATCCTGGCGATGGCGGGGGTTGGTCAGATAGGCGCGGCCATTGCCGTACTGATGAA
AACCCGCAATGCGCGGCTCAAAAAGGTGATTAAAGGCGCGCTTCCGGTCGGACTGCTGGGGATTGGCGAGCCGCTGATTT
TTGGCGTCACGCTGCCATTGGGTAAGCCATTTATTGGCGCTTGCCTCGGCGGCGCGGTAGGCGGGGCGTTGATCAGCTAC
TGGAAAGTGGCGACGGTAATTACCTTTGGTATTTCCGGTTTGCCGCTGGCATTAACAATTGTTGCCGGAAAAGTCCTGTT
CTATCTGTTAGGCTATTTAATAGCGGTAATTGCCGGGTTTATTTTTACCTGGCTGCTGGGGTTCAATGATCCAGAGGAGT
AA

Upstream 100 bases:

>100_bases
TTGATGGCGCTTACGGGGCTGGATGCTGCAGCCGCCAGGGAAAAACTCGCCGCTCATCAGGGCTTTTTAAGGGCGGCGTT
AGAACACTAAAGAGGTGTGT

Downstream 100 bases:

>100_bases
GGTTTGGTCAGCCACGAGCGTCGTGTCGTCTTTTTTGATTTGGATGGAACGTTATATCAACAGGATATGTTCGGCAGTTT
TCTGCGTTATTTGCTGCGCC

Product: PTS system transporter subunit IIBC

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MDKTAALASDILRGIGGEQNILRLENCMTRVRVEVQDDSQLDIPRLKALPGVSGYVKQGEQHQLIVGPGKAAQVVDAMRV
QIAAGGVKPDDAMARTKSEAKAKYKAPMSDALRKLANVFIPLIPAFIASGLITGIINILKRPDIVGDVAVHYPNLLGLMG
IFGSAVFAIMNILVGVNTAKVFGGSQALGGVMAGILSSPQLAQITLFGEALQPGRGGVIAVLLVVALMCWIERQFRKLLP
GSLELILNPLLTTVITGAVAIVALQPLGGWISDAIAHGASWAIDRGGFLVGAVLAGTFLPLVLIGLHQGLVPIHVELVQA
HGYNALFPILAMAGVGQIGAAIAVLMKTRNARLKKVIKGALPVGLLGIGEPLIFGVTLPLGKPFIGACLGGAVGGALISY
WKVATVITFGISGLPLALTIVAGKVLFYLLGYLIAVIAGFIFTWLLGFNDPEE

Sequences:

>Translated_453_residues
MDKTAALASDILRGIGGEQNILRLENCMTRVRVEVQDDSQLDIPRLKALPGVSGYVKQGEQHQLIVGPGKAAQVVDAMRV
QIAAGGVKPDDAMARTKSEAKAKYKAPMSDALRKLANVFIPLIPAFIASGLITGIINILKRPDIVGDVAVHYPNLLGLMG
IFGSAVFAIMNILVGVNTAKVFGGSQALGGVMAGILSSPQLAQITLFGEALQPGRGGVIAVLLVVALMCWIERQFRKLLP
GSLELILNPLLTTVITGAVAIVALQPLGGWISDAIAHGASWAIDRGGFLVGAVLAGTFLPLVLIGLHQGLVPIHVELVQA
HGYNALFPILAMAGVGQIGAAIAVLMKTRNARLKKVIKGALPVGLLGIGEPLIFGVTLPLGKPFIGACLGGAVGGALISY
WKVATVITFGISGLPLALTIVAGKVLFYLLGYLIAVIAGFIFTWLLGFNDPEE
>Mature_453_residues
MDKTAALASDILRGIGGEQNILRLENCMTRVRVEVQDDSQLDIPRLKALPGVSGYVKQGEQHQLIVGPGKAAQVVDAMRV
QIAAGGVKPDDAMARTKSEAKAKYKAPMSDALRKLANVFIPLIPAFIASGLITGIINILKRPDIVGDVAVHYPNLLGLMG
IFGSAVFAIMNILVGVNTAKVFGGSQALGGVMAGILSSPQLAQITLFGEALQPGRGGVIAVLLVVALMCWIERQFRKLLP
GSLELILNPLLTTVITGAVAIVALQPLGGWISDAIAHGASWAIDRGGFLVGAVLAGTFLPLVLIGLHQGLVPIHVELVQA
HGYNALFPILAMAGVGQIGAAIAVLMKTRNARLKKVIKGALPVGLLGIGEPLIFGVTLPLGKPFIGACLGGAVGGALISY
WKVATVITFGISGLPLALTIVAGKVLFYLLGYLIAVIAGFIFTWLLGFNDPEE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788769, Length=474, Percent_Identity=38.3966244725738, Blast_Score=258, Evalue=7e-70,
Organism=Escherichia coli, GI1790159, Length=461, Percent_Identity=29.2841648590022, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI48994906, Length=472, Percent_Identity=27.5423728813559, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI2367362, Length=426, Percent_Identity=26.056338028169, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47232; Mature: 47232

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKTAALASDILRGIGGEQNILRLENCMTRVRVEVQDDSQLDIPRLKALPGVSGYVKQGE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHCCCCCHHHCCCC
QHQLIVGPGKAAQVVDAMRVQIAAGGVKPDDAMARTKSEAKAKYKAPMSDALRKLANVFI
CCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PLIPAFIASGLITGIINILKRPDIVGDVAVHYPNLLGLMGIFGSAVFAIMNILVGVNTAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VFGGSQALGGVMAGILSSPQLAQITLFGEALQPGRGGVIAVLLVVALMCWIERQFRKLLP
HCCCHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GSLELILNPLLTTVITGAVAIVALQPLGGWISDAIAHGASWAIDRGGFLVGAVLAGTFLP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHH
LVLIGLHQGLVPIHVELVQAHGYNALFPILAMAGVGQIGAAIAVLMKTRNARLKKVIKGA
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPVGLLGIGEPLIFGVTLPLGKPFIGACLGGAVGGALISYWKVATVITFGISGLPLALTI
CCCCHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VAGKVLFYLLGYLIAVIAGFIFTWLLGFNDPEE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKTAALASDILRGIGGEQNILRLENCMTRVRVEVQDDSQLDIPRLKALPGVSGYVKQGE
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCCCCCCHHHHCCCCCHHHCCCC
QHQLIVGPGKAAQVVDAMRVQIAAGGVKPDDAMARTKSEAKAKYKAPMSDALRKLANVFI
CCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
PLIPAFIASGLITGIINILKRPDIVGDVAVHYPNLLGLMGIFGSAVFAIMNILVGVNTAK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VFGGSQALGGVMAGILSSPQLAQITLFGEALQPGRGGVIAVLLVVALMCWIERQFRKLLP
HCCCHHHHHHHHHHHHCCCCCEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
GSLELILNPLLTTVITGAVAIVALQPLGGWISDAIAHGASWAIDRGGFLVGAVLAGTFLP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHH
LVLIGLHQGLVPIHVELVQAHGYNALFPILAMAGVGQIGAAIAVLMKTRNARLKKVIKGA
HHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPVGLLGIGEPLIFGVTLPLGKPFIGACLGGAVGGALISYWKVATVITFGISGLPLALTI
CCCCHHCCCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VAGKVLFYLLGYLIAVIAGFIFTWLLGFNDPEE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]