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The map label for this gene is nadB [H]

Identifier: 29140803

GI number: 29140803

Start: 307196

End: 308818

Strand: Reverse

Name: nadB [H]

Synonym: t0269

Alternate gene names: 29140803

Gene position: 308818-307196 (Counterclockwise)

Preceding gene: 29140815

Following gene: 29140801

Centisome position: 6.44

GC content: 55.21

Gene sequence:

>1623_bases
ATGATGACAACTCCTGAACTGTCCTGTGATGTGTTAATTATCGGCAGCGGCGCGGCCGGACTCTCGCTGGCGCTGCGTCT
GGCTGAAAAGCATAAGGTTATCGTCCTGAGTAAAGGGCCGGTGAGCGAGGGCTCCACGTTTTATGCACAGGGCGGTATTG
CCGCCGTGTTTGATGAAACCGACAGCATTGCATCGCATGTCGAAGATACGCTGATTGCCGGCGCCGGAATTTGCGATCGC
CACGCGGTGGAGTTTGTCGCCAGCAACGCTCGTACCTGCGTGCAGTGGCTTATCGATCAGGGCGTCCTGTTTGATACGCA
TGTCCAGCCAAACGGCAAGGAGAGCTATCATCTCACGCGTGAAGGCGGCCATAGCCATCGCCGTATCCTTCATGCCGCCG
ACGCTACCGGAAAGGAAGTCGAAACCACGTTGGTCAGTCGGGCACAAAATCATCCTAATATTCAGGTGCTGGAACGCAGC
AACGCCGTTGATTTAATTATCTCCGATAAAATAGGTCTGCCGGGGCCTCGCCGGGTCGTCGGCGCCTGGATCTGGAATCG
CAATAAAGAGTGGGTTGAAACCTGTCATGCGAAGTCGGTGGTGCTGGCGACCGGCGGCGCCTCAAAGGTGTATCAATATA
CCACTAACCCGGATATTTCCTCCGGTGACGGTATCGCGATGGCCTGGCGCGCGGGCTGTCGGGTCGCTAATCTGGAATTT
AACCAGTTTCATCCCACTGCCCTTTATCACCCGCAGGCGCGTAATTTTCTCCTTACCGAAGCGCTGCGCGGCGAAGGCGC
ATACCTTAAACGTCCGGACGGTTCACGGTTTATGCCGGACGTTGACGAACGCGGCGAACTGGCGCCGCGCGATATTGTCG
CCCGCGCTATTGATCATGAGATGAAACGACTCGGCGCAGACTGTATGTTTCTCGATATCAGCCACAAGCCTGACGATTTT
GTCCGCCAGCACTTCCCGATGATCTATGCGAAACTGCTCGATTTGGGGATGGATTTAACCAAAGAACCGATTCCCATTGT
TCCTGCCGCGCACTATACCTGCGGCGGCGTGGTCGTCGATGATTATGGTCGTACCGATGTAGACGGCTTATATGCCATTG
GCGAAGTCAGTTACACCGGTCTGCACGGCGCTAACCGTATGGCGTCCAACTCGTTACTGGAATGCTTAGTGTACGGCTGG
TCCGCGGCAATGGATATCGACAGGCGAATGCCTTCTGTCCACAGCGTTGACGTCCTTCCCGCCTGGGATGAAAGCCGCGT
GGAGAACGCCGATGAGCGGGTGGTGATTCAGCATAACTGGCACGAACTGCGTCTGCTGATGTGGGACTATGTGGGGATTG
TGCGCACGACGAAACGGCTGGAGCGCGCCCTACGGCGTATTACTATGTTGCAACAAGAGATTGACGAATATTACGCCAAC
TTTCGCGTCTCGAATAATTTACTGGAGCTGCGCAATCTGGTGCAAGTGGCCGAACTGATTGTGCGTTGCGCGATGATGCG
TAAAGAGAGCCGGGGGCTGCACTTTACGCTGGATTATCCGCAACAACTGGCTGAATCAGGCCCATCGATCCTTTCGCCGT
TAACGCCTCACATAAACAGATAA

Upstream 100 bases:

>100_bases
TATGTTTAATAAGCACAACACTTTTGAAATCATAACGTGCTTTTTAGCGCCATATAGTGCTAATCTGCCGCAACCATGTT
TAGTAAATTAAACAAGAACC

Downstream 100 bases:

>100_bases
AATGCCTGGGTCAGCGCCGTATAACTTTCAGAATAATTCTGGTCTGACCCGCGAATTACTAATCTGTCGCTAAAGCACTC
CCCGGCTTGCGGGGAGAATG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKIGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPIVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDVLPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR

Sequences:

>Translated_540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKIGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPIVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDVLPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
>Mature_540_residues
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDETDSIASHVEDTLIAGAGICDR
HAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTREGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERS
NAVDLIISDKIGLPGPRRVVGAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPDDF
VRQHFPMIYAKLLDLGMDLTKEPIPIVPAAHYTCGGVVVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGW
SAAMDIDRRMPSVHSVDVLPAWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=518, Percent_Identity=31.0810810810811, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1788928, Length=540, Percent_Identity=90.3703703703704, Blast_Score=1020, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=529, Percent_Identity=32.1361058601134, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786942, Length=569, Percent_Identity=30.4042179261863, Blast_Score=175, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17550100, Length=539, Percent_Identity=30.4267161410019, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=538, Percent_Identity=29.5539033457249, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI71986328, Length=284, Percent_Identity=27.8169014084507, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=31.294964028777, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322416, Length=557, Percent_Identity=30.3411131059246, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.530612244898, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=483, Percent_Identity=24.4306418219462, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24663005, Length=557, Percent_Identity=30.1615798922801, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI17137288, Length=539, Percent_Identity=30.4267161410019, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24655642, Length=539, Percent_Identity=30.4267161410019, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24655647, Length=539, Percent_Identity=30.4267161410019, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60162; Mature: 60162

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCH
DSIASHVEDTLIAGAGICDRHAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
EGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERSNAVDLIISDKIGLPGPRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHEE
GAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
EEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKRLGADCMFLDISHKPDDFVRQHFPMIYAKLLDLGMDLTKEPIPIVPAAHYTCGGVVVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
DYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAMDIDRRMPSVHSVDVLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
AWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
CCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
CEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHCCCCCCCCC
>Mature Secondary Structure
MMTTPELSCDVLIIGSGAAGLSLALRLAEKHKVIVLSKGPVSEGSTFYAQGGIAAVFDET
CCCCCCCCCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCEEEEECCH
DSIASHVEDTLIAGAGICDRHAVEFVASNARTCVQWLIDQGVLFDTHVQPNGKESYHLTR
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCEEEEC
EGGHSHRRILHAADATGKEVETTLVSRAQNHPNIQVLERSNAVDLIISDKIGLPGPRRVV
CCCCCHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCHHEE
GAWIWNRNKEWVETCHAKSVVLATGGASKVYQYTTNPDISSGDGIAMAWRAGCRVANLEF
EEEEECCCHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCEEEEEEC
NQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGSRFMPDVDERGELAPRDIVARAIDHE
CCCCCCEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MKRLGADCMFLDISHKPDDFVRQHFPMIYAKLLDLGMDLTKEPIPIVPAAHYTCGGVVVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEE
DYGRTDVDGLYAIGEVSYTGLHGANRMASNSLLECLVYGWSAAMDIDRRMPSVHSVDVLP
CCCCCCCCCEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
AWDESRVENADERVVIQHNWHELRLLMWDYVGIVRTTKRLERALRRITMLQQEIDEYYAN
CCCHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLVQVAELIVRCAMMRKESRGLHFTLDYPQQLAESGPSILSPLTPHINR
CEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]