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The map label for this gene is yaeL

Identifier: 29140763

GI number: 29140763

Start: 257871

End: 259223

Strand: Direct

Name: yaeL

Synonym: t0224

Alternate gene names: 29140763

Gene position: 257871-259223 (Clockwise)

Preceding gene: 29140762

Following gene: 29140764

Centisome position: 5.38

GC content: 52.99

Gene sequence:

>1353_bases
ATGCTGAGTATTCTCTGGAATCTGGCTGCATTCATCATCGCACTGGGCGTGCTGATCACTGTGCATGAATTTGGTCATTT
CTGGGTTGCCCGGCGCTGCGGTGTGCGAGTTGAGCGCTTTTCCATCGGTTTTGGCAAAGCGCTCTGGCGTCGTACCGATC
GGTACGGCACCGAGTATGTCATCGCCCTGATCCCGCTGGGCGGCTACGTCAAAATGCTGGATGAGCGCGCCGAACCGGTA
GCGCCGGAATTGCGTCGTCACGCCTTCAATAACAAAACGGTGGGCCAGCGCGCCGCAATTATTGCCGCAGGTCCGGTTGC
GAACTTCATTTTTGCTATCTTTGCTTACTGGCTGGTCTTTATCATCGGCGTCCCTGGCGTTCGTCCGGTCATTGGCGAAA
TAACGCCCAACTCGATTGCTGCGCAGGCGCAAATTGCGCCTGGTACGGAACTTAAAGCCGTTGATGGTATCGAAACCCCT
GATTGGGATGCCGTGCGTTTACAACTGGTGTCCAAAATTGGCGATCAGCAGACGACGGTGAGCGTCGCGCCGTTTGGCAG
CGACCAGCGGCAGGACAAAACGCTTGATTTACGTCACTGGGCATTTGAGCCTGATAAGCAAGATCCTGTATCTTCTTTAG
GGATTCGGCCCCGCGGGCCACAGATTGAACCGGTACTGTCAGAAGTGCAGGCGAATTCCGCAGCCAGTAAAGCGGGTTTG
CAAGCAGGCGACAGGATCGTTAAAGTCGATGGTCAGCCGTTAACGCAGTGGATGAAATTCGTGACGTTTGTACGTGATAA
TCCCGGTAAGCCGTTAGCGTTAGAGATTGAAAGGCAGGGGAGCGCCTTGTCGTTGACGTTAACGCCGGATACGAAATCGG
TTAACGGAAAGGCGGAAGGGTTTGCAGGCGTGGTGCCTAAAATTATTCCTCTGCCGGAAGAGTACAAGACTATACGCCAG
TATGGGCCTTTTAGCGCCATCCTCGAAGCCACGGATAAGACGTGGCAGTTGATGAAGCTGACGGTCAATATGCTGGGTAA
ATTGATAACCGGTGATGTGAAACTGAACAACCTCAGTGGGCCGATTTCTATCGCTCAGGGGGCTGGGATGTCAGCGGAGT
TCGGGGTGATTTATTACCTGATGTTCCTTGCGCTAATCAGCGTGAACTTAGGGATAATTAACCTGTTTCCGCTGCCCGTT
CTTGACGGGGGTCATCTGCTGTTCCTGGCGATTGAAAAGCTTAAGGGCGGGCCGGTATCCGAGCGGGTTCAAGACTTTAG
TTATCGCATTGGCTCGATTTTGCTGGTGTTGTTAATGGGGCTTGCACTTTTCAATGATTTCTCTCGGTTGTAA

Upstream 100 bases:

>100_bases
ATGGCGGCATTCTGGACCGAATTGACAGTCTGACGGCTGCCGTACCGGTCTTTGCTTGCCTGTTGTTACTGGTGTTCAGG
ACGCTCTAACGGAAGGTTTT

Downstream 100 bases:

>100_bases
GAGAGTTAGTTAGGAAGAACGCATAATAACGATGGCGATGAAAAAGTTGCTCATAGCGTCGCTGCTGTTCAGCAGCGCCA
CCGTATACGGTGCTGAAGGG

Product: zinc metallopeptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYVIALIPLGGYVKMLDERAEPV
APELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETP
DWDAVRLQLVSKIGDQQTTVSVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEGFAGVVPKIIPLPEEYKTIRQ
YGPFSAILEATDKTWQLMKLTVNMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Sequences:

>Translated_450_residues
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYVIALIPLGGYVKMLDERAEPV
APELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETP
DWDAVRLQLVSKIGDQQTTVSVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEGFAGVVPKIIPLPEEYKTIRQ
YGPFSAILEATDKTWQLMKLTVNMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL
>Mature_450_residues
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYVIALIPLGGYVKMLDERAEPV
APELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVFIIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETP
DWDAVRLQLVSKIGDQQTTVSVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEGFAGVVPKIIPLPEEYKTIRQ
YGPFSAILEATDKTWQLMKLTVNMLGKLITGDVKLNNLSGPISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPV
LDGGHLLFLAIEKLKGGPVSERVQDFSYRIGSILLVLLMGLALFNDFSRL

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain

Homologues:

Organism=Escherichia coli, GI1786373, Length=450, Percent_Identity=88, Blast_Score=792, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSEP_SALTI (Q8Z9A4)

Other databases:

- EMBL:   AL627266
- EMBL:   AE014613
- RefSeq:   NP_454830.1
- RefSeq:   NP_804105.1
- ProteinModelPortal:   Q8Z9A4
- SMR:   Q8Z9A4
- MEROPS:   M50.004
- GeneID:   1067817
- GeneID:   1246735
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t0224
- KEGG:   sty:STY0246
- HOGENOM:   HBG664087
- OMA:   HGTEFVV
- ProtClustDB:   PRK10779
- BioCyc:   SENT209261:T0224-MONOMER
- BioCyc:   SENT220341:STY0246-MONOMER
- GO:   GO:0006508
- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915
- SMART:   SM00228
- TIGRFAMs:   TIGR00054

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50; SSF50156 PDZ

EC number: NA

Molecular weight: Translated: 49212; Mature: 49212

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50106 PDZ; PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 23-23

Signals:

None

Transmembrane regions:

HASH(0xc29eda4)-; HASH(0xc1795dc)-; HASH(0xc3a4b70)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCEEE
IALIPLGGYVKMLDERAEPVAPELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETPDWDAVRLQLVSKIGDQQTTV
HHCCCCCCCHHCCCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
SVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
EEECCCCCCCCCCCEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCHHHHHHCC
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEG
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCC
FAGVVPKIIPLPEEYKTIRQYGPFSAILEATDKTWQLMKLTVNMLGKLITGDVKLNNLSG
CCCCCHHHCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPVLDGGHLLFLAIEKLKGGPVS
CEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFSYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MLSILWNLAAFIIALGVLITVHEFGHFWVARRCGVRVERFSIGFGKALWRRTDRYGTEYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCCCEEE
IALIPLGGYVKMLDERAEPVAPELRRHAFNNKTVGQRAAIIAAGPVANFIFAIFAYWLVF
EEEECCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
IIGVPGVRPVIGEITPNSIAAQAQIAPGTELKAVDGIETPDWDAVRLQLVSKIGDQQTTV
HHCCCCCCCHHCCCCCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCEEE
SVAPFGSDQRQDKTLDLRHWAFEPDKQDPVSSLGIRPRGPQIEPVLSEVQANSAASKAGL
EEECCCCCCCCCCCEEHHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCHHHHHHCC
QAGDRIVKVDGQPLTQWMKFVTFVRDNPGKPLALEIERQGSALSLTLTPDTKSVNGKAEG
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCC
FAGVVPKIIPLPEEYKTIRQYGPFSAILEATDKTWQLMKLTVNMLGKLITGDVKLNNLSG
CCCCCHHHCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
PISIAQGAGMSAEFGVIYYLMFLALISVNLGIINLFPLPVLDGGHLLFLAIEKLKGGPVS
CEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCEEEEEEEHHCCCCHH
ERVQDFSYRIGSILLVLLMGLALFNDFSRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504