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The map label for this gene is hofB [H]

Identifier: 29140690

GI number: 29140690

Start: 169457

End: 170842

Strand: Reverse

Name: hofB [H]

Synonym: t0149

Alternate gene names: 29140690

Gene position: 170842-169457 (Counterclockwise)

Preceding gene: 29140691

Following gene: 29140689

Centisome position: 3.57

GC content: 55.34

Gene sequence:

>1386_bases
ATGAAAGACGCTCAGCTCAACACCTTGTGTCAACGTCATCAGGCTGTACTGATCAATAGCGCCAGCAACAGTATCACTGT
TGCGGTGGTCGACGCGCCCTCTCATGCGTTATTGGACGCTCTGCATTTCGCCACGCAAAAACAGATTGATATTGTGTGCT
GGACTCGCCAGCAAATGGAAAATCATCGGCATAAGCCGGATCAGGCACCCTCGACAAACGCCGCGAAAGGCGGAGAAACC
GCGGCCCAGCTTCTCAATCAGACATTACGCTCGGCGATGGCGAAACGCGCCTCTGATATCCATCTCGAACCCGGCGCCAG
CCGCTACCGGATAAGGCTCCGCATCGATGGCGTTCTTCATATTCTGCAGGACATCGCCAAAGAGACGGGGCTTGCGCTGA
CGGCCAGATTGAAAGTCCTAGGAAATTTGGACATCGCTGAACATCGGCTGCCGCAGGACGGGCAATTTACCGTTGACCTG
ACGGGCGACAGCATCTCTTTTCGTATCGCCACGCTTCCCTGTAAAGAAGGCGAAAAAGTCGTCTTACGGTTGTTGCATCA
GGTTGAACAAACGCTGGATCTGGACACGCTGGGCATGTACGGGGCACAACTTACCGCTTTTAGACAAGCCCTACAGCAAC
CTCAGGGGCTGGTACTGGTGACTGGCCCCACCGGTAGCGGAAAAACGGTCACGCTATACAGCGCGCTACAAACCCGGAAT
ACGCCGGGTATCAACCTCTGTAGCGTTGAGGATCCGATAGAAATTCCACTGGATGGGATTAACCAGACGCAGATCCATCC
CCGCGCCGGGCTCACCTTTCAAAATGTGCTGCGCGCGCTTTTACGCCAGGACCCTGACATCATCATGGTAGGTGAAATTC
GTGACGGCGACACCGCAGAAATCGCCATTAAGGCGGCACAAACGGGCCATCTGGTACTTTCAACACTCCATACGAACTCC
ACCAGCGAAACCCTGATACGTTTGCAGCAAATAGGCGTAACACGCTGGATGATCTCATCGGCGCTTACGCTGGTGGTAGC
GCAACGCCTGGTAAGGAAGCTTTGCCCGCACTGCAAACAGCGTCTGAGCGATCCCGTGGTGCTATCGCCCAATCTTTGGC
CCAGCGCGCTACCGCGCTGGCAGGCAAACGGCTGTCAGCATTGCTACCACGGCTTTTATGGCCGAACCGCGTTATTCGAA
GTGTTAACCGTTACCCCGGCATTACGTCAGTTAATTGCGAGCGGCGCATCGGCGCAAGCACTGGAAGCCCACCTCCAGCA
GACCGGTACAGGGACGCTTTTTGAAAATGGCTGTCGCGCCGTTGAGCAAGGCATGACGTCCTTTGAAGAGATACTGCGCG
TTCTGGGAATGCCCCATGAGCGTTAA

Upstream 100 bases:

>100_bases
CATTACCGGCTGGACGCGTAACTGCAATATTCAAAGCGACAGCGCGTTACAACAGGCTTGTGAAGATGTCTTCCGTTTTG
ACGCCAACTAAGGAGACAAG

Downstream 100 bases:

>100_bases
ACAGCTCTGGCGCTGGCAAGGCGTTAACGATAAAGGTCAACTGGAACAAGACGTTGTATGGGTGGACAATCGTCTGGCGC
TGATCATCACCCTGCAACAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKDAQLNTLCQRHQAVLINSASNSITVAVVDAPSHALLDALHFATQKQIDIVCWTRQQMENHRHKPDQAPSTNAAKGGET
AAQLLNQTLRSAMAKRASDIHLEPGASRYRIRLRIDGVLHILQDIAKETGLALTARLKVLGNLDIAEHRLPQDGQFTVDL
TGDSISFRIATLPCKEGEKVVLRLLHQVEQTLDLDTLGMYGAQLTAFRQALQQPQGLVLVTGPTGSGKTVTLYSALQTRN
TPGINLCSVEDPIEIPLDGINQTQIHPRAGLTFQNVLRALLRQDPDIIMVGEIRDGDTAEIAIKAAQTGHLVLSTLHTNS
TSETLIRLQQIGVTRWMISSALTLVVAQRLVRKLCPHCKQRLSDPVVLSPNLWPSALPRWQANGCQHCYHGFYGRTALFE
VLTVTPALRQLIASGASAQALEAHLQQTGTGTLFENGCRAVEQGMTSFEEILRVLGMPHER

Sequences:

>Translated_461_residues
MKDAQLNTLCQRHQAVLINSASNSITVAVVDAPSHALLDALHFATQKQIDIVCWTRQQMENHRHKPDQAPSTNAAKGGET
AAQLLNQTLRSAMAKRASDIHLEPGASRYRIRLRIDGVLHILQDIAKETGLALTARLKVLGNLDIAEHRLPQDGQFTVDL
TGDSISFRIATLPCKEGEKVVLRLLHQVEQTLDLDTLGMYGAQLTAFRQALQQPQGLVLVTGPTGSGKTVTLYSALQTRN
TPGINLCSVEDPIEIPLDGINQTQIHPRAGLTFQNVLRALLRQDPDIIMVGEIRDGDTAEIAIKAAQTGHLVLSTLHTNS
TSETLIRLQQIGVTRWMISSALTLVVAQRLVRKLCPHCKQRLSDPVVLSPNLWPSALPRWQANGCQHCYHGFYGRTALFE
VLTVTPALRQLIASGASAQALEAHLQQTGTGTLFENGCRAVEQGMTSFEEILRVLGMPHER
>Mature_461_residues
MKDAQLNTLCQRHQAVLINSASNSITVAVVDAPSHALLDALHFATQKQIDIVCWTRQQMENHRHKPDQAPSTNAAKGGET
AAQLLNQTLRSAMAKRASDIHLEPGASRYRIRLRIDGVLHILQDIAKETGLALTARLKVLGNLDIAEHRLPQDGQFTVDL
TGDSISFRIATLPCKEGEKVVLRLLHQVEQTLDLDTLGMYGAQLTAFRQALQQPQGLVLVTGPTGSGKTVTLYSALQTRN
TPGINLCSVEDPIEIPLDGINQTQIHPRAGLTFQNVLRALLRQDPDIIMVGEIRDGDTAEIAIKAAQTGHLVLSTLHTNS
TSETLIRLQQIGVTRWMISSALTLVVAQRLVRKLCPHCKQRLSDPVVLSPNLWPSALPRWQANGCQHCYHGFYGRTALFE
VLTVTPALRQLIASGASAQALEAHLQQTGTGTLFENGCRAVEQGMTSFEEILRVLGMPHER

Specific function: Unknown

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=459, Percent_Identity=69.7167755991285, Blast_Score=671, Evalue=0.0,
Organism=Escherichia coli, GI1789723, Length=403, Percent_Identity=43.1761786600496, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI87082188, Length=346, Percent_Identity=30.635838150289, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 50521; Mature: 50521

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDAQLNTLCQRHQAVLINSASNSITVAVVDAPSHALLDALHFATQKQIDIVCWTRQQME
CCCCHHHHHHHHCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCEEEEEECHHHHH
NHRHKPDQAPSTNAAKGGETAAQLLNQTLRSAMAKRASDIHLEPGASRYRIRLRIDGVLH
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEHHHHH
ILQDIAKETGLALTARLKVLGNLDIAEHRLPQDGQFTVDLTGDSISFRIATLPCKEGEKV
HHHHHHHHHCCCCEEEHHHHCCCCHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCHHH
VLRLLHQVEQTLDLDTLGMYGAQLTAFRQALQQPQGLVLVTGPTGSGKTVTLYSALQTRN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEHHHCCC
TPGINLCSVEDPIEIPLDGINQTQIHPRAGLTFQNVLRALLRQDPDIIMVGEIRDGDTAE
CCCCEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEE
IAIKAAQTGHLVLSTLHTNSTSETLIRLQQIGVTRWMISSALTLVVAQRLVRKLCPHCKQ
EEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSDPVVLSPNLWPSALPRWQANGCQHCYHGFYGRTALFEVLTVTPALRQLIASGASAQA
HCCCCEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LEAHLQQTGTGTLFENGCRAVEQGMTSFEEILRVLGMPHER
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKDAQLNTLCQRHQAVLINSASNSITVAVVDAPSHALLDALHFATQKQIDIVCWTRQQME
CCCCHHHHHHHHCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCEEEEEECHHHHH
NHRHKPDQAPSTNAAKGGETAAQLLNQTLRSAMAKRASDIHLEPGASRYRIRLRIDGVLH
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEHHHHH
ILQDIAKETGLALTARLKVLGNLDIAEHRLPQDGQFTVDLTGDSISFRIATLPCKEGEKV
HHHHHHHHHCCCCEEEHHHHCCCCHHHHCCCCCCCEEEEEECCCEEEEEEECCCCCCHHH
VLRLLHQVEQTLDLDTLGMYGAQLTAFRQALQQPQGLVLVTGPTGSGKTVTLYSALQTRN
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEEHHHCCC
TPGINLCSVEDPIEIPLDGINQTQIHPRAGLTFQNVLRALLRQDPDIIMVGEIRDGDTAE
CCCCEEECCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEE
IAIKAAQTGHLVLSTLHTNSTSETLIRLQQIGVTRWMISSALTLVVAQRLVRKLCPHCKQ
EEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSDPVVLSPNLWPSALPRWQANGCQHCYHGFYGRTALFEVLTVTPALRQLIASGASAQA
HCCCCEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LEAHLQQTGTGTLFENGCRAVEQGMTSFEEILRVLGMPHER
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503 [H]