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The map label for this gene is cca [H]

Identifier: 28572951

GI number: 28572951

Start: 918020

End: 919456

Strand: Direct

Name: cca [H]

Synonym: TW810

Alternate gene names: 28572951

Gene position: 918020-919456 (Clockwise)

Preceding gene: 28572950

Following gene: NA

Centisome position: 99.14

GC content: 50.31

Gene sequence:

>1437_bases
GTGAAGTATACAAGCATGTTTAAATTAGCGCTATCGCGCCTGCAAAAACTGTCACATGAAGACTCGGTCCTTGCTCTTAC
AGCGGCATTCTGTGCCAAGAATTATGAGCTTGCCCTGGTCGGTGGACCGGTTAGAGATTGTTTCCTTGACAGGCCGGTTG
TTGACCTTGATTTTGCCACAAACGCAACGCCGCAGGAGACTCTGAGGCTTGTAAAACCCATTTCCTCGGGCTGTTGGACA
ACAGGGGCACGCTACGGAACCGTTTCTGCTCGCGTGAAGGGCAGGGTGGTTGAGATAACCACATATCGAAGCGACAACTA
CTCGGCGAATACCCGCAAACCTAAGGTAAGTTTTGGAGGCTCCCTTGAAGAAGACTTAATTCGGCGCGACTTCACGGTCA
ACGCAATGGCATTCAGGGTGCCTAGCGGGCAACTGGTTGATATAAGTTCCGGTCTTCAAGACATTGCCAACAAAAGACTT
GCGACCCCTATAAAACCGGAACTATCTTTTGCAGACGACCCCCTGCGCATGTTGAGGGCTGCTCGATTGTCTTCTCAATT
GGGTTTCAAAATATCGGAGAACAGCCTTCTTGCCATGAAAGACTGTGCCAAGAGGTTAAAAATCGTCTCGGCAGAGCGTG
TAAGGGGTGAGTTTGAAAAACTGCTTTGCTGTGCAGATCCCACGGGCAGTTTACGGGTGCTCGTCAATACCGGTTTGATT
AATCATTTTTTGCCAGAGATATCCGCTCTGCGCTTGGAAATTGATGAGCACTTCCACCACAAAGATGTGTATGAACATTC
CCTCATTGTTCTCCGCCAAGCAATCAAATTGGAACAGGAGCGCTTCCGTAATGGGCCCGACCTGGTCCTGCGTCTGGCGG
CGCTCATGCACGATATTGGCAAGCCTGCAACCCGTCGTAAACATCCGGACGGGTCTGTGACCTTTTATCACCACGATACG
GTTGGTGCAAAAATGGCCTCGAAGCGCCTAGCGGCAATGCGTTTTGACAAGAATGTTATCTTTGCGGTCACTCGCCTGAT
TCAGCTTCATCTTCGTTTTTTTGGCTACGGTGACCAATCGTGGACCGACTCCGCTGTGAGGCGCTATGTGAGAGATGCTG
GCCCCCAACTGGAGCGCCTACATATACTGACCCGCGCCGATGTTACCACCAGAAACCAGAATAAAGCTCGGCGCCTCAGG
GCCGCCTATGACAACCTCGAAGAGCGAATTGCCAAACTTCGAGAACAAGAAGAGTTAGACGCGATAAGACCTGAATTAAA
TGGTGAAGAGATTATGAGCCTCCTAGATCTAAAGCCTGGCAAAGAAGTTGGGGTGGCCTATAAATACCTTCTCGACCTCA
GGCTGGACAATGGACCCATTGGCAAACAGGAATCAGCTAGAAAGCTCTTATTGTGGTGGAAATCTGAGAGGCGGTAG

Upstream 100 bases:

>100_bases
TATGCTGCCAATATACTCGTATTTTCTTGTTATAACCCGCTGCCGGGAAATCAGGTTTTTTTTCAAATGAAGTACAAACG
GGTTGTTTTGGAGCGCTCTT

Downstream 100 bases:

>100_bases
CACACCCCAATCCCACGGCGCGGGCTCGCGTGGGGGCAAATCAGGGGGCAAATCACAGGCCCGAGGGTGGCACACCCATT
TTAAGAAGAATCCGCCTAAG

Product: RNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKYTSMFKLALSRLQKLSHEDSVLALTAAFCAKNYELALVGGPVRDCFLDRPVVDLDFATNATPQETLRLVKPISSGCWT
TGARYGTVSARVKGRVVEITTYRSDNYSANTRKPKVSFGGSLEEDLIRRDFTVNAMAFRVPSGQLVDISSGLQDIANKRL
ATPIKPELSFADDPLRMLRAARLSSQLGFKISENSLLAMKDCAKRLKIVSAERVRGEFEKLLCCADPTGSLRVLVNTGLI
NHFLPEISALRLEIDEHFHHKDVYEHSLIVLRQAIKLEQERFRNGPDLVLRLAALMHDIGKPATRRKHPDGSVTFYHHDT
VGAKMASKRLAAMRFDKNVIFAVTRLIQLHLRFFGYGDQSWTDSAVRRYVRDAGPQLERLHILTRADVTTRNQNKARRLR
AAYDNLEERIAKLREQEELDAIRPELNGEEIMSLLDLKPGKEVGVAYKYLLDLRLDNGPIGKQESARKLLLWWKSERR

Sequences:

>Translated_478_residues
MKYTSMFKLALSRLQKLSHEDSVLALTAAFCAKNYELALVGGPVRDCFLDRPVVDLDFATNATPQETLRLVKPISSGCWT
TGARYGTVSARVKGRVVEITTYRSDNYSANTRKPKVSFGGSLEEDLIRRDFTVNAMAFRVPSGQLVDISSGLQDIANKRL
ATPIKPELSFADDPLRMLRAARLSSQLGFKISENSLLAMKDCAKRLKIVSAERVRGEFEKLLCCADPTGSLRVLVNTGLI
NHFLPEISALRLEIDEHFHHKDVYEHSLIVLRQAIKLEQERFRNGPDLVLRLAALMHDIGKPATRRKHPDGSVTFYHHDT
VGAKMASKRLAAMRFDKNVIFAVTRLIQLHLRFFGYGDQSWTDSAVRRYVRDAGPQLERLHILTRADVTTRNQNKARRLR
AAYDNLEERIAKLREQEELDAIRPELNGEEIMSLLDLKPGKEVGVAYKYLLDLRLDNGPIGKQESARKLLLWWKSERR
>Mature_478_residues
MKYTSMFKLALSRLQKLSHEDSVLALTAAFCAKNYELALVGGPVRDCFLDRPVVDLDFATNATPQETLRLVKPISSGCWT
TGARYGTVSARVKGRVVEITTYRSDNYSANTRKPKVSFGGSLEEDLIRRDFTVNAMAFRVPSGQLVDISSGLQDIANKRL
ATPIKPELSFADDPLRMLRAARLSSQLGFKISENSLLAMKDCAKRLKIVSAERVRGEFEKLLCCADPTGSLRVLVNTGLI
NHFLPEISALRLEIDEHFHHKDVYEHSLIVLRQAIKLEQERFRNGPDLVLRLAALMHDIGKPATRRKHPDGSVTFYHHDT
VGAKMASKRLAAMRFDKNVIFAVTRLIQLHLRFFGYGDQSWTDSAVRRYVRDAGPQLERLHILTRADVTTRNQNKARRLR
AAYDNLEERIAKLREQEELDAIRPELNGEEIMSLLDLKPGKEVGVAYKYLLDLRLDNGPIGKQESARKLLLWWKSERR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=228, Percent_Identity=32.4561403508772, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789436, Length=329, Percent_Identity=30.3951367781155, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081691, Length=225, Percent_Identity=30.2222222222222, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71995920, Length=227, Percent_Identity=26.8722466960352, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=222, Percent_Identity=31.5315315315315, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281360112, Length=206, Percent_Identity=30.0970873786408, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21357337, Length=206, Percent_Identity=30.0970873786408, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54211; Mature: 54211

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTSMFKLALSRLQKLSHEDSVLALTAAFCAKNYELALVGGPVRDCFLDRPVVDLDFAT
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCEEEECCC
NATPQETLRLVKPISSGCWTTGARYGTVSARVKGRVVEITTYRSDNYSANTRKPKVSFGG
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCCCCCCCCCCCCCC
SLEEDLIRRDFTVNAMAFRVPSGQLVDISSGLQDIANKRLATPIKPELSFADDPLRMLRA
CHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
ARLSSQLGFKISENSLLAMKDCAKRLKIVSAERVRGEFEKLLCCADPTGSLRVLVNTGLI
HHHHHHCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCHH
NHFLPEISALRLEIDEHFHHKDVYEHSLIVLRQAIKLEQERFRNGPDLVLRLAALMHDIG
HHHHHHHHHHEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
KPATRRKHPDGSVTFYHHDTVGAKMASKRLAAMRFDKNVIFAVTRLIQLHLRFFGYGDQS
CCHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
WTDSAVRRYVRDAGPQLERLHILTRADVTTRNQNKARRLRAAYDNLEERIAKLREQEELD
CHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIRPELNGEEIMSLLDLKPGKEVGVAYKYLLDLRLDNGPIGKQESARKLLLWWKSERR
HHCCCCCHHHHHHHHCCCCCCHHHHHEEEHEEEEECCCCCCCCHHHCEEEEEECCCCC
>Mature Secondary Structure
MKYTSMFKLALSRLQKLSHEDSVLALTAAFCAKNYELALVGGPVRDCFLDRPVVDLDFAT
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCEEEECCC
NATPQETLRLVKPISSGCWTTGARYGTVSARVKGRVVEITTYRSDNYSANTRKPKVSFGG
CCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCCCCCCCCCCCCCC
SLEEDLIRRDFTVNAMAFRVPSGQLVDISSGLQDIANKRLATPIKPELSFADDPLRMLRA
CHHHHHHHCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
ARLSSQLGFKISENSLLAMKDCAKRLKIVSAERVRGEFEKLLCCADPTGSLRVLVNTGLI
HHHHHHCCCEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCHH
NHFLPEISALRLEIDEHFHHKDVYEHSLIVLRQAIKLEQERFRNGPDLVLRLAALMHDIG
HHHHHHHHHHEEEHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHC
KPATRRKHPDGSVTFYHHDTVGAKMASKRLAAMRFDKNVIFAVTRLIQLHLRFFGYGDQS
CCHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
WTDSAVRRYVRDAGPQLERLHILTRADVTTRNQNKARRLRAAYDNLEERIAKLREQEELD
CHHHHHHHHHHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIRPELNGEEIMSLLDLKPGKEVGVAYKYLLDLRLDNGPIGKQESARKLLLWWKSERR
HHCCCCCHHHHHHHHCCCCCCHHHHHEEEHEEEEECCCCCCCCHHHCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA