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The map label for this gene is purB [H]

Identifier: 28572941

GI number: 28572941

Start: 907280

End: 908725

Strand: Direct

Name: purB [H]

Synonym: TW800

Alternate gene names: 28572941

Gene position: 907280-908725 (Clockwise)

Preceding gene: 28572930

Following gene: 28572942

Centisome position: 97.98

GC content: 46.96

Gene sequence:

>1446_bases
GTGTCAGAGGATGAATATGATTCACTGTCTCCCTTAGATGGCAGGTATGGCCCAGCTGTGCGAGCATTGCGCGGTTTTCT
GTCTGAGAGTGCGCTTATTCGCGAGCGTATTAGGGTTGAAATCGGCTGGGTATTTTTTTTGTGGAGGAAAGCGGTTTTTG
GACTTGCAGAACCCACAAAGGAAGATGAGAGGTTTCTTTCATCGATAGTGCATGATGCGCATAAAAACATACCGCGGGTC
AAGAAGATTGAAGAGCGGATACAGCACGATGTGAAAGCCGTCGAGATCTTCCTGCGAGAGAGATTTACGCAGCAGGGTCT
GGGCAATATAGTCGAGCTACTACATTTCGCGTGCACAAGTGAAGACATAAACAACCTGGCGTACGCACTGAACATGAAGG
CAGCTCTCTCAGAAGTCATCCTGCCCTCGATTGATGAACTTATAGGACACCTAGAAAAGATGTCTCAGAAATATGCGGGA
GATGCAATGCTCGCGCGCACACACGGACAACCCGCAACACCAACAACTCTTGGAAAGGAGCTATTGGTGTTTGTCTACCG
CCTGAAAAGGCAACGGAAAAATCTAGAAGATGTGCAATTTCTTGGGAAATTCTCTGGTGCGACCGGGACATTTTCAGCTC
ATTGCGTAGCCGCCCCAAATTACCCGTGGCAAGAGTGGGCAAAAGAATTTGTGAACAGTTTTGGCCTCATTTATAACCCC
ATTACCACTCAAATTGAGCCACATGACTGGCAATGCGAGCTGCTTGAGAGAGTGGGGCATATCTGTCGAATCCTCCACAA
TCTGGCAACCGATATGTGGAGCTATATCTCATTCGGATATTTTCAGCAAGGCTTTATATCAGAATCTGTTGGCTCCTCAA
CTATGCCACACAAGGTCAATCCCATCCGCTTTGAAAATGCCGAGGCAAATCTGGAAATGGCTTGCGGAATAATCTCATGC
ATTGTTGCAAGTCTGTCTACAAGCAGGCTTCAGCGGGACTTGAGCGACTCCAGTGTCCAGAGAAATATAGGTGTCGCCCT
GGGGCATTCACTTGTAGCGATCGATAACTTGAAAAGAGGTCTTGCTGGTATTCGCCTGAATAAAAATCGCCTAACTGAAG
ACCTCAATAACAACTGGGAAGTAATTGCGGAGGCGGTACAAACAACCATCCGCGCCGAAATACTGGCATGTAAAAGCAAT
GAGGCTAACCCTTATGAAAGGCTCAAAGATTTCACACGAAACAAGAAAATTACGAAACACACTCTCGATGAATTTATAAC
TAACTTGGACCTAAGCGAGCATGCAAAAAAACGACTTTTAGAATTAACACCAAGCAAATATACTGGCTTGGCAGAGCGCA
TGGTGCAGGCACATAGCTCAGGCACTGGGGGCACCAAAAATAGCATTGGCACTGGGGGCACCAAAAATAGCTCAGGTGCT
GGGTGA

Upstream 100 bases:

>100_bases
GACTACTCTACAGTTGCCGTAATCCGTAAAATCCCCATTAAGGAAGATGTAGAAAACATTACAGATAAGAACACATGACA
AATAAAAGTAAAATGTAGGG

Downstream 100 bases:

>100_bases
AAATGCTTGCCTGCAAAACTATGGCAAGCTTTCGGTTGCTCAAGCCTGAGGAGGAATAGATTTGCCAGCAACAATTCTTA
TTGGTGCCCAGTGGGGGGAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSEDEYDSLSPLDGRYGPAVRALRGFLSESALIRERIRVEIGWVFFLWRKAVFGLAEPTKEDERFLSSIVHDAHKNIPRV
KKIEERIQHDVKAVEIFLRERFTQQGLGNIVELLHFACTSEDINNLAYALNMKAALSEVILPSIDELIGHLEKMSQKYAG
DAMLARTHGQPATPTTLGKELLVFVYRLKRQRKNLEDVQFLGKFSGATGTFSAHCVAAPNYPWQEWAKEFVNSFGLIYNP
ITTQIEPHDWQCELLERVGHICRILHNLATDMWSYISFGYFQQGFISESVGSSTMPHKVNPIRFENAEANLEMACGIISC
IVASLSTSRLQRDLSDSSVQRNIGVALGHSLVAIDNLKRGLAGIRLNKNRLTEDLNNNWEVIAEAVQTTIRAEILACKSN
EANPYERLKDFTRNKKITKHTLDEFITNLDLSEHAKKRLLELTPSKYTGLAERMVQAHSSGTGGTKNSIGTGGTKNSSGA
G

Sequences:

>Translated_481_residues
MSEDEYDSLSPLDGRYGPAVRALRGFLSESALIRERIRVEIGWVFFLWRKAVFGLAEPTKEDERFLSSIVHDAHKNIPRV
KKIEERIQHDVKAVEIFLRERFTQQGLGNIVELLHFACTSEDINNLAYALNMKAALSEVILPSIDELIGHLEKMSQKYAG
DAMLARTHGQPATPTTLGKELLVFVYRLKRQRKNLEDVQFLGKFSGATGTFSAHCVAAPNYPWQEWAKEFVNSFGLIYNP
ITTQIEPHDWQCELLERVGHICRILHNLATDMWSYISFGYFQQGFISESVGSSTMPHKVNPIRFENAEANLEMACGIISC
IVASLSTSRLQRDLSDSSVQRNIGVALGHSLVAIDNLKRGLAGIRLNKNRLTEDLNNNWEVIAEAVQTTIRAEILACKSN
EANPYERLKDFTRNKKITKHTLDEFITNLDLSEHAKKRLLELTPSKYTGLAERMVQAHSSGTGGTKNSIGTGGTKNSSGA
G
>Mature_480_residues
SEDEYDSLSPLDGRYGPAVRALRGFLSESALIRERIRVEIGWVFFLWRKAVFGLAEPTKEDERFLSSIVHDAHKNIPRVK
KIEERIQHDVKAVEIFLRERFTQQGLGNIVELLHFACTSEDINNLAYALNMKAALSEVILPSIDELIGHLEKMSQKYAGD
AMLARTHGQPATPTTLGKELLVFVYRLKRQRKNLEDVQFLGKFSGATGTFSAHCVAAPNYPWQEWAKEFVNSFGLIYNPI
TTQIEPHDWQCELLERVGHICRILHNLATDMWSYISFGYFQQGFISESVGSSTMPHKVNPIRFENAEANLEMACGIISCI
VASLSTSRLQRDLSDSSVQRNIGVALGHSLVAIDNLKRGLAGIRLNKNRLTEDLNNNWEVIAEAVQTTIRAEILACKSNE
ANPYERLKDFTRNKKITKHTLDEFITNLDLSEHAKKRLLELTPSKYTGLAERMVQAHSSGTGGTKNSIGTGGTKNSSGAG

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=292, Percent_Identity=24.6575342465753, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557269, Length=292, Percent_Identity=24.6575342465753, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1787376, Length=451, Percent_Identity=43.0155210643016, Blast_Score=387, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53921; Mature: 53790

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDEYDSLSPLDGRYGPAVRALRGFLSESALIRERIRVEIGWVFFLWRKAVFGLAEPTK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EDERFLSSIVHDAHKNIPRVKKIEERIQHDVKAVEIFLRERFTQQGLGNIVELLHFACTS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EDINNLAYALNMKAALSEVILPSIDELIGHLEKMSQKYAGDAMLARTHGQPATPTTLGKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHH
LLVFVYRLKRQRKNLEDVQFLGKFSGATGTFSAHCVAAPNYPWQEWAKEFVNSFGLIYNP
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEECC
ITTQIEPHDWQCELLERVGHICRILHNLATDMWSYISFGYFQQGFISESVGSSTMPHKVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PIRFENAEANLEMACGIISCIVASLSTSRLQRDLSDSSVQRNIGVALGHSLVAIDNLKRG
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LAGIRLNKNRLTEDLNNNWEVIAEAVQTTIRAEILACKSNEANPYERLKDFTRNKKITKH
HHCCEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHH
TLDEFITNLDLSEHAKKRLLELTPSKYTGLAERMVQAHSSGTGGTKNSIGTGGTKNSSGA
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
G
C
>Mature Secondary Structure 
SEDEYDSLSPLDGRYGPAVRALRGFLSESALIRERIRVEIGWVFFLWRKAVFGLAEPTK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EDERFLSSIVHDAHKNIPRVKKIEERIQHDVKAVEIFLRERFTQQGLGNIVELLHFACTS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EDINNLAYALNMKAALSEVILPSIDELIGHLEKMSQKYAGDAMLARTHGQPATPTTLGKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCCCCCCCHHHHHH
LLVFVYRLKRQRKNLEDVQFLGKFSGATGTFSAHCVAAPNYPWQEWAKEFVNSFGLIYNP
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEECC
ITTQIEPHDWQCELLERVGHICRILHNLATDMWSYISFGYFQQGFISESVGSSTMPHKVN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PIRFENAEANLEMACGIISCIVASLSTSRLQRDLSDSSVQRNIGVALGHSLVAIDNLKRG
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LAGIRLNKNRLTEDLNNNWEVIAEAVQTTIRAEILACKSNEANPYERLKDFTRNKKITKH
HHCCEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHH
TLDEFITNLDLSEHAKKRLLELTPSKYTGLAERMVQAHSSGTGGTKNSIGTGGTKNSSGA
HHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA