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The map label for this gene is gshR3 [H]

Identifier: 28378490

GI number: 28378490

Start: 1648551

End: 1649900

Strand: Direct

Name: gshR3 [H]

Synonym: lp_1822

Alternate gene names: 28378490

Gene position: 1648551-1649900 (Clockwise)

Preceding gene: 28378489

Following gene: 28378492

Centisome position: 49.83

GC content: 48.37

Gene sequence:

>1350_bases
ATGGCAGATTATGACACAATTTTTATCGGTAGCGGGCACGCAACGTGGCATGCGGCAGTGGCCCTAGCACATGCGCAACA
TAAGGTAGCAATCATTGAAGAAGATACCATCGCCGGGACGTGCACTAACTTTGGCTGTGATGCTAAGATTCTGTTAGACG
GTCCCTTCGAGCTGACTGAACAACTCAAACAATATCAAGGGATTGGCGTCAACACAACGCCCACCATCGATTGGTCACAG
TTGATGGCCTATAAGCAACAAGTCATTCAACCTTTATCGGTACAAATGACGGCCGTCTTCAAACAATTGGGGATTACAAT
TATTACCGGTCATGGTGAGCTGACTGATACCCACACTGTTCAAGTAGCGGACAGCACGTATACAGCGGATACCATCGTCA
TCGGGACCGGACAACGACCAGCCAAGCTGGCTATCCCGGGTGCGGACCTGATGCATGACAGTCGTGATTTTCTTGACCTA
CCAACCATGCCTAAACGGTTGACCTTAATTGGTGCTGGAATCATCTCGCTGGAATTTGCCAACATGGCCGTGTTACTAGG
GTCAGAAGTGCATATTATTGAGTTTGCCGATCGAGCACTACCCGCATTTTATTCAGAACACGTTAAAAAGATGATCACAC
ATCTACAGGCAGCCGGGGTGCACTTCCACTTTGGCGAAGCGCTTAGCCAAGTGACTAAAACGGCCACAGGGTTAATGGCA
ACCACTGCCAGCGGCCTGAAGATTGAGAGTGATGATATCATTGCGGCCACTGGTCGGATTCCAAACATCGAACACTTAGG
ACTAACGAAAGTTGGCATTAAGACCGACCGGCACGGCATTATCGTCGACGACCATTTGCGGACAAGCATTCCAAACATTT
ACGCCAGTGGTGATGTCATTAGTAAGACTTTGCCGAAGCTAACCCCGACCGCGATCTTTGAATCTAACTACATTGCCGGC
CAACTGCTTGGTAGCACTGCCGCCATTGATTATCCGGTCATTCCAGCGGTCGTCTTTACGCTACCAAGAATCGCCCAAGT
CGGTGTCAGTGTCGAGGCTGCCCAGCATGATACTGAACACTTTCACGTTCAAGCGCTGCCATATGGTAAACTGTTAGCCT
TTCAGTATCAAAACGAGGTCGACGCTGATTTACAATTAGTCTTTGACCAAGAGAATTACCTCGTCGGTGCCTCAATCTAT
GGTAATGGCGCACTAGACCTGATTAACTTACTGACCATGATTATTGCTGATCACGTCTCCGCCACAACGTTAAGTCAGAA
GATTTTTGCCTTTCCAAGTGCTTCAGTGGGGATCATTGATATGTTGACACCACTACTACACCATGACTAG

Upstream 100 bases:

>100_bases
AGATTATTACCGCATTATCACAGGATTTGGCCTAAACGGGCCTTTTTATTGACCGTTTCAATCGCGTGCGACTGAAATAT
AATTTTAGGAGGAATCTCTA

Downstream 100 bases:

>100_bases
TTATTAAACCAACTAACTAATTTAAAGATACTGGGTATGTTGCTGACTACCCAGTATCTTTTTGTCGTTTCAGATTAACT
CACTGACTGAGGACACCTAA

Product: glutathione reductase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MADYDTIFIGSGHATWHAAVALAHAQHKVAIIEEDTIAGTCTNFGCDAKILLDGPFELTEQLKQYQGIGVNTTPTIDWSQ
LMAYKQQVIQPLSVQMTAVFKQLGITIITGHGELTDTHTVQVADSTYTADTIVIGTGQRPAKLAIPGADLMHDSRDFLDL
PTMPKRLTLIGAGIISLEFANMAVLLGSEVHIIEFADRALPAFYSEHVKKMITHLQAAGVHFHFGEALSQVTKTATGLMA
TTASGLKIESDDIIAATGRIPNIEHLGLTKVGIKTDRHGIIVDDHLRTSIPNIYASGDVISKTLPKLTPTAIFESNYIAG
QLLGSTAAIDYPVIPAVVFTLPRIAQVGVSVEAAQHDTEHFHVQALPYGKLLAFQYQNEVDADLQLVFDQENYLVGASIY
GNGALDLINLLTMIIADHVSATTLSQKIFAFPSASVGIIDMLTPLLHHD

Sequences:

>Translated_449_residues
MADYDTIFIGSGHATWHAAVALAHAQHKVAIIEEDTIAGTCTNFGCDAKILLDGPFELTEQLKQYQGIGVNTTPTIDWSQ
LMAYKQQVIQPLSVQMTAVFKQLGITIITGHGELTDTHTVQVADSTYTADTIVIGTGQRPAKLAIPGADLMHDSRDFLDL
PTMPKRLTLIGAGIISLEFANMAVLLGSEVHIIEFADRALPAFYSEHVKKMITHLQAAGVHFHFGEALSQVTKTATGLMA
TTASGLKIESDDIIAATGRIPNIEHLGLTKVGIKTDRHGIIVDDHLRTSIPNIYASGDVISKTLPKLTPTAIFESNYIAG
QLLGSTAAIDYPVIPAVVFTLPRIAQVGVSVEAAQHDTEHFHVQALPYGKLLAFQYQNEVDADLQLVFDQENYLVGASIY
GNGALDLINLLTMIIADHVSATTLSQKIFAFPSASVGIIDMLTPLLHHD
>Mature_448_residues
ADYDTIFIGSGHATWHAAVALAHAQHKVAIIEEDTIAGTCTNFGCDAKILLDGPFELTEQLKQYQGIGVNTTPTIDWSQL
MAYKQQVIQPLSVQMTAVFKQLGITIITGHGELTDTHTVQVADSTYTADTIVIGTGQRPAKLAIPGADLMHDSRDFLDLP
TMPKRLTLIGAGIISLEFANMAVLLGSEVHIIEFADRALPAFYSEHVKKMITHLQAAGVHFHFGEALSQVTKTATGLMAT
TASGLKIESDDIIAATGRIPNIEHLGLTKVGIKTDRHGIIVDDHLRTSIPNIYASGDVISKTLPKLTPTAIFESNYIAGQ
LLGSTAAIDYPVIPAVVFTLPRIAQVGVSVEAAQHDTEHFHVQALPYGKLLAFQYQNEVDADLQLVFDQENYLVGASIYG
NGALDLINLLTMIIADHVSATTLSQKIFAFPSASVGIIDMLTPLLHHD

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=27.7056277056277, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI91199540, Length=455, Percent_Identity=27.6923076923077, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI33519430, Length=393, Percent_Identity=25.4452926208651, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519428, Length=393, Percent_Identity=25.4452926208651, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519426, Length=393, Percent_Identity=25.4452926208651, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI148277065, Length=393, Percent_Identity=25.4452926208651, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI148277071, Length=393, Percent_Identity=25.4452926208651, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045266, Length=393, Percent_Identity=26.2086513994911, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI22035672, Length=473, Percent_Identity=25.5813953488372, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI291045268, Length=309, Percent_Identity=26.8608414239482, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1786307, Length=448, Percent_Identity=27.2321428571429, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1789915, Length=336, Percent_Identity=30.3571428571429, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=25.5458515283843, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=25.764192139738, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789065, Length=229, Percent_Identity=28.3842794759825, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=455, Percent_Identity=26.3736263736264, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=329, Percent_Identity=30.3951367781155, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=329, Percent_Identity=30.3951367781155, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=469, Percent_Identity=25.7995735607676, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=348, Percent_Identity=26.4367816091954, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325166, Length=383, Percent_Identity=28.1984334203655, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321091, Length=460, Percent_Identity=24.7826086956522, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=22.9787234042553, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21358499, Length=455, Percent_Identity=29.8901098901099, Blast_Score=162, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24640549, Length=376, Percent_Identity=29.2553191489362, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640553, Length=376, Percent_Identity=29.2553191489362, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640551, Length=376, Percent_Identity=29.2553191489362, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17737741, Length=380, Percent_Identity=28.4210526315789, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48419; Mature: 48288

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYDTIFIGSGHATWHAAVALAHAQHKVAIIEEDTIAGTCTNFGCDAKILLDGPFELTE
CCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCEEEEECCCHHHHH
QLKQYQGIGVNTTPTIDWSQLMAYKQQVIQPLSVQMTAVFKQLGITIITGHGELTDTHTV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCCEEE
QVADSTYTADTIVIGTGQRPAKLAIPGADLMHDSRDFLDLPTMPKRLTLIGAGIISLEFA
EEECCCEECCEEEEECCCCCCEEECCCHHHHCCCCCCCCCCCCCCEEEEEECCEEEEEEC
NMAVLLGSEVHIIEFADRALPAFYSEHVKKMITHLQAAGVHFHFGEALSQVTKTATGLMA
CEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHE
TTASGLKIESDDIIAATGRIPNIEHLGLTKVGIKTDRHGIIVDDHLRTSIPNIYASGDVI
ECCCCCEECCCCEEEECCCCCCCCCCCEEEECEEECCCCEEEECHHHHCCCCEEECCCHH
SKTLPKLTPTAIFESNYIAGQLLGSTAAIDYPVIPAVVFTLPRIAQVGVSVEAAQHDTEH
HHHHCCCCCHHEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCE
FHVQALPYGKLLAFQYQNEVDADLQLVFDQENYLVGASIYGNGALDLINLLTMIIADHVS
EEEEEECCCEEEEEEECCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHCC
ATTLSQKIFAFPSASVGIIDMLTPLLHHD
HHHHHHHHEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
ADYDTIFIGSGHATWHAAVALAHAQHKVAIIEEDTIAGTCTNFGCDAKILLDGPFELTE
CCCCEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCEEEECCCCCCEEEEECCCHHHHH
QLKQYQGIGVNTTPTIDWSQLMAYKQQVIQPLSVQMTAVFKQLGITIITGHGELTDTHTV
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCCEEE
QVADSTYTADTIVIGTGQRPAKLAIPGADLMHDSRDFLDLPTMPKRLTLIGAGIISLEFA
EEECCCEECCEEEEECCCCCCEEECCCHHHHCCCCCCCCCCCCCCEEEEEECCEEEEEEC
NMAVLLGSEVHIIEFADRALPAFYSEHVKKMITHLQAAGVHFHFGEALSQVTKTATGLMA
CEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHE
TTASGLKIESDDIIAATGRIPNIEHLGLTKVGIKTDRHGIIVDDHLRTSIPNIYASGDVI
ECCCCCEECCCCEEEECCCCCCCCCCCEEEECEEECCCCEEEECHHHHCCCCEEECCCHH
SKTLPKLTPTAIFESNYIAGQLLGSTAAIDYPVIPAVVFTLPRIAQVGVSVEAAQHDTEH
HHHHCCCCCHHEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCE
FHVQALPYGKLLAFQYQNEVDADLQLVFDQENYLVGASIYGNGALDLINLLTMIIADHVS
EEEEEECCCEEEEEEECCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHCC
ATTLSQKIFAFPSASVGIIDMLTPLLHHD
HHHHHHHHEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]