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The map label for this gene is pyrAA2 [H]

Identifier: 28378457

GI number: 28378457

Start: 1614564

End: 1615646

Strand: Direct

Name: pyrAA2 [H]

Synonym: lp_1783

Alternate gene names: 28378457

Gene position: 1614564-1615646 (Clockwise)

Preceding gene: 28378456

Following gene: 28378458

Centisome position: 48.8

GC content: 48.48

Gene sequence:

>1083_bases
ATGGCAGACCGCTATTTGATTCTTGAAGATGGCAGCGCCTATTTAGGTGAAGGCTTTGGCTCGCCAGCGGTTTCTACCGG
AGAAATCGTCGTCAACACGAGTATGACGGGCTATCAAGAAATTATTACTAATCAAATTTATCATAACCAAATCGTTGCTT
TTACGCAGCCAACCATTGGGAGCTATGGGATCAACCACGATAGCTACGAATCAATTTTACCGACCGTTAAGGGGGTCGTT
GTTCGGGATGTCGCTAGCATTTCGACCAACCGGCAGCGGCGCTGGTCACTGGATCAATATTTAAAGCAACAAAATATTCC
TGGCATCAGCCATATTGATACACGGCACTTAGCGAAGCAACTCCGAGCAAGTGGCCCGATGAAAGCCAGTATCGTAGACG
TTGCAGATGCCCATGCCTTTGATCAATTGGGTGCGACCGTGCTGACTAATCAACAGGTGGCAGCGGTCGCAACACCGAAA
CCATTTCCTAATCCGGGGACGGGGCTCAATGTCGTGGTCATCGACTTTGGCCTCAAACACGGGATTTTGCGCGAACTTAG
TAAGCGTGCTTGTAACGTCACGGTCTTACCTTATACGGCAACGACTGAGGAAATTTTAAACTTAGATCCAGACGGCGTCA
TTCTATCGACTGGGCCCGGCAAACCGCAGGACTTACCAGTCAGTGTGACTGAGATGATCAAGAACATTCAAAATCGGGTG
CCACTGTTTGCCATCGGACTGGGCCACGAACTATTTGCGATGGCCAATGGCGCTCAAATTATGAAATTATCACCTGAACA
TCATGGGGCTAACCACCCAATCCGCGAAGTGATTACGAACCAAATTATTTATGCAGCTCAGGGCCAGGGGTTCGCGGTAG
ATGCGGATACTGTTGATCGTAATAAGTTAATCACGACATTTGTTGACTTGATCGATGGGACGATTCAAGGACTGCGTCTA
CGGGACTTTCCAGCGTTTTCAGTGCAATTTTTCCCAGATGGCGCTCCTGGTCCGACTGAAACGCGCGATATTTTTGATGA
ATTTGTTGAGTCGATGCAACAAGCAAGGGGGCCAATCTGGTGA

Upstream 100 bases:

>100_bases
CAAGAACGCATCAAAGTGACGGTCAATGAAATCGACGGTCACGATGGTATTGAAATTATTAACTAGTACGACAAAGTCAA
ACAGATGAGAAGGGATCGCA

Downstream 100 bases:

>100_bases
ACAATCAAGCCTTACAAAAAGTGTTGATTATCGGCGCTGGGCATAATGACATTGGCCGAGAGGGACAACACGACGCGGCG
GTGACCCAAATCGGTGCGGC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain; CPS-P [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MADRYLILEDGSAYLGEGFGSPAVSTGEIVVNTSMTGYQEIITNQIYHNQIVAFTQPTIGSYGINHDSYESILPTVKGVV
VRDVASISTNRQRRWSLDQYLKQQNIPGISHIDTRHLAKQLRASGPMKASIVDVADAHAFDQLGATVLTNQQVAAVATPK
PFPNPGTGLNVVVIDFGLKHGILRELSKRACNVTVLPYTATTEEILNLDPDGVILSTGPGKPQDLPVSVTEMIKNIQNRV
PLFAIGLGHELFAMANGAQIMKLSPEHHGANHPIREVITNQIIYAAQGQGFAVDADTVDRNKLITTFVDLIDGTIQGLRL
RDFPAFSVQFFPDGAPGPTETRDIFDEFVESMQQARGPIW

Sequences:

>Translated_360_residues
MADRYLILEDGSAYLGEGFGSPAVSTGEIVVNTSMTGYQEIITNQIYHNQIVAFTQPTIGSYGINHDSYESILPTVKGVV
VRDVASISTNRQRRWSLDQYLKQQNIPGISHIDTRHLAKQLRASGPMKASIVDVADAHAFDQLGATVLTNQQVAAVATPK
PFPNPGTGLNVVVIDFGLKHGILRELSKRACNVTVLPYTATTEEILNLDPDGVILSTGPGKPQDLPVSVTEMIKNIQNRV
PLFAIGLGHELFAMANGAQIMKLSPEHHGANHPIREVITNQIIYAAQGQGFAVDADTVDRNKLITTFVDLIDGTIQGLRL
RDFPAFSVQFFPDGAPGPTETRDIFDEFVESMQQARGPIW
>Mature_359_residues
ADRYLILEDGSAYLGEGFGSPAVSTGEIVVNTSMTGYQEIITNQIYHNQIVAFTQPTIGSYGINHDSYESILPTVKGVVV
RDVASISTNRQRRWSLDQYLKQQNIPGISHIDTRHLAKQLRASGPMKASIVDVADAHAFDQLGATVLTNQQVAAVATPKP
FPNPGTGLNVVVIDFGLKHGILRELSKRACNVTVLPYTATTEEILNLDPDGVILSTGPGKPQDLPVSVTEMIKNIQNRVP
LFAIGLGHELFAMANGAQIMKLSPEHHGANHPIREVITNQIIYAAQGQGFAVDADTVDRNKLITTFVDLIDGTIQGLRLR
DFPAFSVQFFPDGAPGPTETRDIFDEFVESMQQARGPIW

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=362, Percent_Identity=32.0441988950276, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI21361331, Length=372, Percent_Identity=29.3010752688172, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI169790915, Length=372, Percent_Identity=29.3010752688172, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=38.8739946380697, Blast_Score=260, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI193204318, Length=365, Percent_Identity=33.1506849315069, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324878, Length=367, Percent_Identity=33.5149863760218, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322331, Length=398, Percent_Identity=29.3969849246231, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI45555749, Length=378, Percent_Identity=35.1851851851852, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24642586, Length=378, Percent_Identity=35.1851851851852, Blast_Score=183, Evalue=1e-46,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39227; Mature: 39096

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRYLILEDGSAYLGEGFGSPAVSTGEIVVNTSMTGYQEIITNQIYHNQIVAFTQPTIG
CCCCEEEEECCCHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEEEEECCCCC
SYGINHDSYESILPTVKGVVVRDVASISTNRQRRWSLDQYLKQQNIPGISHIDTRHLAKQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHCCCCCCCCCHHHHHHHH
LRASGPMKASIVDVADAHAFDQLGATVLTNQQVAAVATPKPFPNPGTGLNVVVIDFGLKH
HHHCCCCCEEEEHHHHHHHHHHHCCEEECCCCEEEEECCCCCCCCCCCCEEEEEECCHHH
GILRELSKRACNVTVLPYTATTEEILNLDPDGVILSTGPGKPQDLPVSVTEMIKNIQNRV
HHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCC
PLFAIGLGHELFAMANGAQIMKLSPEHHGANHPIREVITNQIIYAAQGQGFAVDADTVDR
CEEEEECCCHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCEEEEECCCCEEEECCCCCC
NKLITTFVDLIDGTIQGLRLRDFPAFSVQFFPDGAPGPTETRDIFDEFVESMQQARGPIW
HHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADRYLILEDGSAYLGEGFGSPAVSTGEIVVNTSMTGYQEIITNQIYHNQIVAFTQPTIG
CCCEEEEECCCHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEEEEECCCCC
SYGINHDSYESILPTVKGVVVRDVASISTNRQRRWSLDQYLKQQNIPGISHIDTRHLAKQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHCCCCCCCCCHHHHHHHH
LRASGPMKASIVDVADAHAFDQLGATVLTNQQVAAVATPKPFPNPGTGLNVVVIDFGLKH
HHHCCCCCEEEEHHHHHHHHHHHCCEEECCCCEEEEECCCCCCCCCCCCEEEEEECCHHH
GILRELSKRACNVTVLPYTATTEEILNLDPDGVILSTGPGKPQDLPVSVTEMIKNIQNRV
HHHHHHHHHHCCEEEEECCCCHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCC
PLFAIGLGHELFAMANGAQIMKLSPEHHGANHPIREVITNQIIYAAQGQGFAVDADTVDR
CEEEEECCCHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCEEEEECCCCEEEECCCCCC
NKLITTFVDLIDGTIQGLRLRDFPAFSVQFFPDGAPGPTETRDIFDEFVESMQQARGPIW
HHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8982065; 12566566; 10852872 [H]