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The map label for this gene is pstF [H]

Identifier: 28377594

GI number: 28377594

Start: 670692

End: 671594

Strand: Direct

Name: pstF [H]

Synonym: lp_0733

Alternate gene names: 28377594

Gene position: 670692-671594 (Clockwise)

Preceding gene: 28377593

Following gene: 28377596

Centisome position: 20.27

GC content: 44.41

Gene sequence:

>903_bases
ATGAAAAAAGCACTGACACTCGGCGCATTGGCAGTAACGATGACCATTTTACTAGCTGGTTGCGGCAGTAGCTCAGCCAC
AAGTACGAAGAGTACTAGTAGCAGTAGTGGTAGTTCCAAGACTACTAAGATCGTTGCAACTGGTTCAACCGCTTTACAAC
CATTAGTGGAACAGGCTGGACAGGCTTACCAGAACGAACATGCTAACGTGACCATTAACGTTCAGGGTGGTGGTTCAGGA
GCAGGCTTAAGTCAGGTGGCGAAGGGTTCAGTCAATATTGGGAACTCTGATTTATTTGCACAGGAACAAAAGGGGTTAGA
CGCCAAAGGGTTAGTTGACCATAAAGTGGCTGTTGTGGGGATGGCACCCGTTATTAATAAGGATGCCGGTGTCACTAATA
TTTCAAAAGAACAACTTATCAAGATTTTCCAAGGCAAGATCACTAACTGGAAAGATGTTGGCGGTAAGGACGAAAAGATT
ACCGTCGTTAACCGTGCTCAAGGTAGTGGGACCCGGGCGACGTTTGAAAAATGGGGATTAGACAACGCGAAGGTCGCAAC
TAGCCAAGAACAAGATTCGAATGGGACCGTTCAAAAAATTGTTAGCACCACGCCTGGTGCCATCAGTTACTTAGCATTCT
CATACGTTAAGAGTGACGTTCAGGCGCTTTCTATTGATAACGTCAAACCAACCGAAGCTAACGTAGCGACGAACAAGTGG
AAGATTTGGGCTTATGAACACATGTATACTAAGGGGCAACCAACTGGGGAGTTGAAGAACTTCTTAACTTACATGACTTC
TAAAGCCGTTCAAGGTAGTTTAGTTGAGAAATTAGGATATATTCCATTGACTTCGATGAAGGTTGATCGTGGCCAATCTG
GTGCAATCACTAATTTAAAATAA

Upstream 100 bases:

>100_bases
CTCCATCACTAGTCGTAGCACTCCTACCCAGTTTTGCTATGATGGTTGCCGGGTTGGTTGCTTAATTTTGATAGTCAATT
GAAGAAGGGGAGTTTCAATT

Downstream 100 bases:

>100_bases
ATAACAGACAAAAAGTCGAGCGATAGCTCGACTTTTTTTATGGGCCGCAATTAGTGCAACGCGGACTTTAACAATGAAAA
AACAAATCATCGGCTCAAGT

Product: phosphate ABC transporter, substrate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKKALTLGALAVTMTILLAGCGSSSATSTKSTSSSSGSSKTTKIVATGSTALQPLVEQAGQAYQNEHANVTINVQGGGSG
AGLSQVAKGSVNIGNSDLFAQEQKGLDAKGLVDHKVAVVGMAPVINKDAGVTNISKEQLIKIFQGKITNWKDVGGKDEKI
TVVNRAQGSGTRATFEKWGLDNAKVATSQEQDSNGTVQKIVSTTPGAISYLAFSYVKSDVQALSIDNVKPTEANVATNKW
KIWAYEHMYTKGQPTGELKNFLTYMTSKAVQGSLVEKLGYIPLTSMKVDRGQSGAITNLK

Sequences:

>Translated_300_residues
MKKALTLGALAVTMTILLAGCGSSSATSTKSTSSSSGSSKTTKIVATGSTALQPLVEQAGQAYQNEHANVTINVQGGGSG
AGLSQVAKGSVNIGNSDLFAQEQKGLDAKGLVDHKVAVVGMAPVINKDAGVTNISKEQLIKIFQGKITNWKDVGGKDEKI
TVVNRAQGSGTRATFEKWGLDNAKVATSQEQDSNGTVQKIVSTTPGAISYLAFSYVKSDVQALSIDNVKPTEANVATNKW
KIWAYEHMYTKGQPTGELKNFLTYMTSKAVQGSLVEKLGYIPLTSMKVDRGQSGAITNLK
>Mature_300_residues
MKKALTLGALAVTMTILLAGCGSSSATSTKSTSSSSGSSKTTKIVATGSTALQPLVEQAGQAYQNEHANVTINVQGGGSG
AGLSQVAKGSVNIGNSDLFAQEQKGLDAKGLVDHKVAVVGMAPVINKDAGVTNISKEQLIKIFQGKITNWKDVGGKDEKI
TVVNRAQGSGTRATFEKWGLDNAKVATSQEQDSNGTVQKIVSTTPGAISYLAFSYVKSDVQALSIDNVKPTEANVATNKW
KIWAYEHMYTKGQPTGELKNFLTYMTSKAVQGSLVEKLGYIPLTSMKVDRGQSGAITNLK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=224, Percent_Identity=27.6785714285714, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 31480; Mature: 31480

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALTLGALAVTMTILLAGCGSSSATSTKSTSSSSGSSKTTKIVATGSTALQPLVEQAG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHH
QAYQNEHANVTINVQGGGSGAGLSQVAKGSVNIGNSDLFAQEQKGLDAKGLVDHKVAVVG
HHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEE
MAPVINKDAGVTNISKEQLIKIFQGKITNWKDVGGKDEKITVVNRAQGSGTRATFEKWGL
ECHHCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHCC
DNAKVATSQEQDSNGTVQKIVSTTPGAISYLAFSYVKSDVQALSIDNVKPTEANVATNKW
CCCEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
KIWAYEHMYTKGQPTGELKNFLTYMTSKAVQGSLVEKLGYIPLTSMKVDRGQSGAITNLK
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKALTLGALAVTMTILLAGCGSSSATSTKSTSSSSGSSKTTKIVATGSTALQPLVEQAG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHH
QAYQNEHANVTINVQGGGSGAGLSQVAKGSVNIGNSDLFAQEQKGLDAKGLVDHKVAVVG
HHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEEEE
MAPVINKDAGVTNISKEQLIKIFQGKITNWKDVGGKDEKITVVNRAQGSGTRATFEKWGL
ECHHCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHCC
DNAKVATSQEQDSNGTVQKIVSTTPGAISYLAFSYVKSDVQALSIDNVKPTEANVATNKW
CCCEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCE
KIWAYEHMYTKGQPTGELKNFLTYMTSKAVQGSLVEKLGYIPLTSMKVDRGQSGAITNLK
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8760913; 8969508; 9384377 [H]