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The map label for this gene is pts10B [H]

Identifier: 28377468

GI number: 28377468

Start: 561614

End: 562132

Strand: Direct

Name: pts10B [H]

Synonym: lp_0587

Alternate gene names: 28377468

Gene position: 561614-562132 (Clockwise)

Preceding gene: 28377467

Following gene: 28377469

Centisome position: 16.98

GC content: 47.4

Gene sequence:

>519_bases
ATGACGATGGATATTCGTTTAGCACGCATTGATAGTCGGCTACTCCACGGGCAAGTTGCCACGGTGTGGACCAAGACGGT
CAGTCCTAACCGTATTTTGGTCGTCTCAGACGAAGTTGCCCAGGATGCACTTCGCAAGATTCTAATTGTGCAAGCCGCGC
CCCCTGGTGTGAAAGCTAATGTGATTACAGTTGACAAAATGATCGAGATTTATCAAGACTCGCTATTTGATGAAGTAAAG
CCACTAATCCTAACTGATACGCCCCAGAACATGGCGCGCTTAGTAGCGGGGGGACTTGATTTTAGCAAAACGGGCATTGA
TATTGGTAGTCTAGCTTTTTCAGCGGGCATGGTGATGGTCACCAATGCTATTGCAATCGGTCAAGCCGAAGCACGGGCAT
TATATGCGTTACAAGCAGCGGGATTAAAGGTGTTTGCGCAGAAAGTCCCGACGGATAAACGGGTTGATGTGATGCCGCTA
TTAGCTAAAAATGGCTTTATGGCGCCAGATAAGTATTAA

Upstream 100 bases:

>100_bases
ATAAGCCAATTGATGAGGTGATTGCCCACCTTGAAGAGACTGGAAAGATTGGGATTAAGCATCTCAAGTTGTTACAGGAC
GATGGGGAAGATGAGCCGCT

Downstream 100 bases:

>100_bases
CAAAGTTATGATGGACAGTGGTGTGTCGGTTATGGGCACCACTGTTCTTTCATTTTGGCTAGGTTTCTAAGGATTTACCA
AAAATACAGCCGTAAACTTT

Product: mannose PTS, EIIB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MTMDIRLARIDSRLLHGQVATVWTKTVSPNRILVVSDEVAQDALRKILIVQAAPPGVKANVITVDKMIEIYQDSLFDEVK
PLILTDTPQNMARLVAGGLDFSKTGIDIGSLAFSAGMVMVTNAIAIGQAEARALYALQAAGLKVFAQKVPTDKRVDVMPL
LAKNGFMAPDKY

Sequences:

>Translated_172_residues
MTMDIRLARIDSRLLHGQVATVWTKTVSPNRILVVSDEVAQDALRKILIVQAAPPGVKANVITVDKMIEIYQDSLFDEVK
PLILTDTPQNMARLVAGGLDFSKTGIDIGSLAFSAGMVMVTNAIAIGQAEARALYALQAAGLKVFAQKVPTDKRVDVMPL
LAKNGFMAPDKY
>Mature_171_residues
TMDIRLARIDSRLLHGQVATVWTKTVSPNRILVVSDEVAQDALRKILIVQAAPPGVKANVITVDKMIEIYQDSLFDEVKP
LILTDTPQNMARLVAGGLDFSKTGIDIGSLAFSAGMVMVTNAIAIGQAEARALYALQAAGLKVFAQKVPTDKRVDVMPLL
AKNGFMAPDKY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=161, Percent_Identity=43.4782608695652, Blast_Score=137, Evalue=3e-34,
Organism=Escherichia coli, GI87082217, Length=158, Percent_Identity=31.6455696202532, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18595; Mature: 18464

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDIRLARIDSRLLHGQVATVWTKTVSPNRILVVSDEVAQDALRKILIVQAAPPGVKAN
CCCEEEEHHHHHHHHCCCEEEEEEECCCCCEEEEEECHHHHHHHHHEEEEECCCCCCEEE
VITVDKMIEIYQDSLFDEVKPLILTDTPQNMARLVAGGLDFSKTGIDIGSLAFSAGMVMV
EEEHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHH
TNAIAIGQAEARALYALQAAGLKVFAQKVPTDKRVDVMPLLAKNGFMAPDKY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCC
>Mature Secondary Structure 
TMDIRLARIDSRLLHGQVATVWTKTVSPNRILVVSDEVAQDALRKILIVQAAPPGVKAN
CCEEEEHHHHHHHHCCCEEEEEEECCCCCEEEEEECHHHHHHHHHEEEEECCCCCCEEE
VITVDKMIEIYQDSLFDEVKPLILTDTPQNMARLVAGGLDFSKTGIDIGSLAFSAGMVMV
EEEHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHH
TNAIAIGQAEARALYALQAAGLKVFAQKVPTDKRVDVMPLLAKNGFMAPDKY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]