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The map label for this gene is nadE

Identifier: 28377448

GI number: 28377448

Start: 522053

End: 522880

Strand: Direct

Name: nadE

Synonym: lp_0566

Alternate gene names: 28377448

Gene position: 522053-522880 (Clockwise)

Preceding gene: 28377447

Following gene: 28377449

Centisome position: 15.78

GC content: 48.19

Gene sequence:

>828_bases
ATGCGGCCATTACAAGCTGAGATTATTAAAGCGTTACACGTTGCACCAACGATTGATCCAGAAGTTGAGATTCGTCGAAG
TATTGATTTTTTGAAGGCTTATTTAACTAAAAATACCTTTTTGAAGACCTATGTGCTTGGCATCTCTGGCGGTCAAGATT
CCACGTTAGCTGGAAAATTGACGGAGATGGCCATTACCGAAATGCGGCAGGAAACGGGTGATGATCGCTATCAATTCATC
GCAGTCCGGTTACCATATGGCAATCAAGCGGATGAAGCGGATGCGATGGCAGCGATTGATTTTATGCAAGCCGACGTGAC
GGATCGGGTGGATATTCAACCGGCTACGGATGCCATGGTAACTGCATTGGAAGCTAACCAATTGACGATTCATGATTTCA
ACAAGGGCAATATTAAAGCGCGCCAACGCATGATCGTGCAATATGGGATTGCTGGTGAAATGCACGGCGCTGTCGTGGGA
ACCGACCATGCAGCCGAAGCTGTTACTGGTTTTTATACCAAGTACGGTGACGGTGGTGCGGATATTGTGCCACTATGGCG
ATTGAACAAACGTCAAGGTAAACAGATGCTGGCCGCTTTGGATGCGCCCAAGCATTTGTATGACAAAGTCCCGACCGCTG
ACTTGGAAGAAGATCGGCCAGCATTACCTGATGAAGTCGCGTTAGGTGTTCGTTACGACGACATCGACGATTACTTGGAA
GGCCGGACGGTCAGCGATGCTGCGGCCGAAAAGATTGAGGCGTGGTACTTAAAGACTGCCCACAAACGCCACGCGGCCAT
CACTGTGTTTGATGATTTTTGGAAATAA

Upstream 100 bases:

>100_bases
ACCCGGTCGACTTGTCACAAAAACTTTATGATCATAAAATGAATATTATTAACACGGTCAGAGACCACGTTAACCATAAA
GATGAGGAGGAAGCTTACTA

Downstream 100 bases:

>100_bases
GCTAGTTAGCTGATAATCGATTAGGATTACGGCTAACCTGATTAAAAAGGCGACTTGAAGATTATTCAGCTTCAGGTCGC
CTTTTTGCATAGTCACATAA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MRPLQAEIIKALHVAPTIDPEVEIRRSIDFLKAYLTKNTFLKTYVLGISGGQDSTLAGKLTEMAITEMRQETGDDRYQFI
AVRLPYGNQADEADAMAAIDFMQADVTDRVDIQPATDAMVTALEANQLTIHDFNKGNIKARQRMIVQYGIAGEMHGAVVG
TDHAAEAVTGFYTKYGDGGADIVPLWRLNKRQGKQMLAALDAPKHLYDKVPTADLEEDRPALPDEVALGVRYDDIDDYLE
GRTVSDAAAEKIEAWYLKTAHKRHAAITVFDDFWK

Sequences:

>Translated_275_residues
MRPLQAEIIKALHVAPTIDPEVEIRRSIDFLKAYLTKNTFLKTYVLGISGGQDSTLAGKLTEMAITEMRQETGDDRYQFI
AVRLPYGNQADEADAMAAIDFMQADVTDRVDIQPATDAMVTALEANQLTIHDFNKGNIKARQRMIVQYGIAGEMHGAVVG
TDHAAEAVTGFYTKYGDGGADIVPLWRLNKRQGKQMLAALDAPKHLYDKVPTADLEEDRPALPDEVALGVRYDDIDDYLE
GRTVSDAAAEKIEAWYLKTAHKRHAAITVFDDFWK
>Mature_275_residues
MRPLQAEIIKALHVAPTIDPEVEIRRSIDFLKAYLTKNTFLKTYVLGISGGQDSTLAGKLTEMAITEMRQETGDDRYQFI
AVRLPYGNQADEADAMAAIDFMQADVTDRVDIQPATDAMVTALEANQLTIHDFNKGNIKARQRMIVQYGIAGEMHGAVVG
TDHAAEAVTGFYTKYGDGGADIVPLWRLNKRQGKQMLAALDAPKHLYDKVPTADLEEDRPALPDEVALGVRYDDIDDYLE
GRTVSDAAAEKIEAWYLKTAHKRHAAITVFDDFWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=272, Percent_Identity=65.4411764705882, Blast_Score=360, Evalue=1e-101,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_LACPL (Q88Z14)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784340.1
- HSSP:   P18843
- ProteinModelPortal:   Q88Z14
- SMR:   Q88Z14
- GeneID:   1064235
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0566
- NMPDR:   fig|220668.1.peg.474
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   LPLA220668:LP_0566-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30590; Mature: 30590

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLQAEIIKALHVAPTIDPEVEIRRSIDFLKAYLTKNTFLKTYVLGISGGQDSTLAGKL
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHH
TEMAITEMRQETGDDRYQFIAVRLPYGNQADEADAMAAIDFMQADVTDRVDIQPATDAMV
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
TALEANQLTIHDFNKGNIKARQRMIVQYGIAGEMHGAVVGTDHAAEAVTGFYTKYGDGGA
EEEECCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCC
DIVPLWRLNKRQGKQMLAALDAPKHLYDKVPTADLEEDRPALPDEVALGVRYDDIDDYLE
CEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCHHEECEEECCHHHHHC
GRTVSDAAAEKIEAWYLKTAHKRHAAITVFDDFWK
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure
MRPLQAEIIKALHVAPTIDPEVEIRRSIDFLKAYLTKNTFLKTYVLGISGGQDSTLAGKL
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHH
TEMAITEMRQETGDDRYQFIAVRLPYGNQADEADAMAAIDFMQADVTDRVDIQPATDAMV
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
TALEANQLTIHDFNKGNIKARQRMIVQYGIAGEMHGAVVGTDHAAEAVTGFYTKYGDGGA
EEEECCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCC
DIVPLWRLNKRQGKQMLAALDAPKHLYDKVPTADLEEDRPALPDEVALGVRYDDIDDYLE
CEEEEECCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCHHEECEEECCHHHHHC
GRTVSDAAAEKIEAWYLKTAHKRHAAITVFDDFWK
CCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566