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The map label for this gene is hprT [H]

Identifier: 28377430

GI number: 28377430

Start: 495761

End: 496303

Strand: Direct

Name: hprT [H]

Synonym: lp_0546

Alternate gene names: 28377430

Gene position: 495761-496303 (Clockwise)

Preceding gene: 28377429

Following gene: 28377431

Centisome position: 14.99

GC content: 40.88

Gene sequence:

>543_bases
ATGAACAACGACATTGAACGAGTACTTTATAGTCGCGAGGATATCCATCAGGTTGCACAAAAATTAGGAAAAGAACTCAC
AACTGCATATGCTGGTAAGAACCCATTAATTATTTGCGTACTCAAGGGTGCCGTTTTATTTACCACGGACATTATTCGTG
AAATGGATATTTACGCGGATCTTGATTTTATTAATTTATCAAGTTACGGTAATGAAACGATCTCTAGTGGTGAGGTTCAA
CTAACGAAGGACTTAGATGCTGATGTGACGGGTCGTGATGTGTTAGTCATTGAAGACATCATTGATACCGGGCGGACGTT
GAAGTTCTTGATCGATTTGTTGAAGCGGCGCGATGTGAACTCAGTTAAGGTCTGCACGTTATTGGATAAACCAGCTGGTC
GACTCGTTGATATTAAGGCAGATTATATTGGTTTTGAAGTACCGAATGAGTTTGTGGTCGGTTACGGTCTTGACTATGCA
GAACGCTATCGGAACTTGCCGTACGTTGGGATTTTAAAACCTGCGATTTACGAGCATAAATAA

Upstream 100 bases:

>100_bases
CTGTGGGTGGTCGGTCATAAGTTTAGCTATCGTACGACTGGTACCCAGACAGTATTTTTAGCCTTAAAACACGAGAGTTG
AAAGGAGTACATCTGAAACG

Downstream 100 bases:

>100_bases
TTGGTCAATGATAGTCAAATGTGCTATTATTTAGACTATCGAAGTACTGTATATAAAGTTTCGATGAAGGAGGCACATAT
GAACAATCGACGCAATGGAC

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNNDIERVLYSREDIHQVAQKLGKELTTAYAGKNPLIICVLKGAVLFTTDIIREMDIYADLDFINLSSYGNETISSGEVQ
LTKDLDADVTGRDVLVIEDIIDTGRTLKFLIDLLKRRDVNSVKVCTLLDKPAGRLVDIKADYIGFEVPNEFVVGYGLDYA
ERYRNLPYVGILKPAIYEHK

Sequences:

>Translated_180_residues
MNNDIERVLYSREDIHQVAQKLGKELTTAYAGKNPLIICVLKGAVLFTTDIIREMDIYADLDFINLSSYGNETISSGEVQ
LTKDLDADVTGRDVLVIEDIIDTGRTLKFLIDLLKRRDVNSVKVCTLLDKPAGRLVDIKADYIGFEVPNEFVVGYGLDYA
ERYRNLPYVGILKPAIYEHK
>Mature_180_residues
MNNDIERVLYSREDIHQVAQKLGKELTTAYAGKNPLIICVLKGAVLFTTDIIREMDIYADLDFINLSSYGNETISSGEVQ
LTKDLDADVTGRDVLVIEDIIDTGRTLKFLIDLLKRRDVNSVKVCTLLDKPAGRLVDIKADYIGFEVPNEFVVGYGLDYA
ERYRNLPYVGILKPAIYEHK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=179, Percent_Identity=36.3128491620112, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI9910262, Length=179, Percent_Identity=34.6368715083799, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI87081689, Length=172, Percent_Identity=41.8604651162791, Blast_Score=157, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17510817, Length=183, Percent_Identity=32.7868852459016, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20319; Mature: 20319

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDIERVLYSREDIHQVAQKLGKELTTAYAGKNPLIICVLKGAVLFTTDIIREMDIYAD
CCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
LDFINLSSYGNETISSGEVQLTKDLDADVTGRDVLVIEDIIDTGRTLKFLIDLLKRRDVN
CCEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCCC
SVKVCTLLDKPAGRLVDIKADYIGFEVPNEFVVGYGLDYAERYRNLPYVGILKPAIYEHK
CEEEEEEECCCCCCEEEEEEEEECEECCCCEEEECCCCHHHHHCCCCEEEEECCHHHCCC
>Mature Secondary Structure
MNNDIERVLYSREDIHQVAQKLGKELTTAYAGKNPLIICVLKGAVLFTTDIIREMDIYAD
CCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
LDFINLSSYGNETISSGEVQLTKDLDADVTGRDVLVIEDIIDTGRTLKFLIDLLKRRDVN
CCEEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHCCCC
SVKVCTLLDKPAGRLVDIKADYIGFEVPNEFVVGYGLDYAERYRNLPYVGILKPAIYEHK
CEEEEEEECCCCCCEEEEEEEEECEECCCCEEEECCCCHHHHHCCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]