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The map label for this gene is mesJ

Identifier: 28377429

GI number: 28377429

Start: 494395

End: 495741

Strand: Direct

Name: mesJ

Synonym: lp_0545

Alternate gene names: 28377429

Gene position: 494395-495741 (Clockwise)

Preceding gene: 28377428

Following gene: 28377430

Centisome position: 14.94

GC content: 50.04

Gene sequence:

>1347_bases
ATGACACCAATTCAAAAGTTCAATCGGCAATTAGCTGCAGCCAAACTGTTATCACCACAGCAAACAGTGGTGGTAGCGGT
GTCGACTGGGGTCGATTCAATGGTATTGTTGCATTTGTTGCAACGATTGCCAGCAGCGGAACGGCCACGGGTAGTGGTGG
CTCATGTTAATCATCATTTACGCGAACAAAGTCAAATGGAAGCGGACTACTTGCGACAATATTGCCAGCAGCAAAACTTG
AAGCTAGTCATGGCCGATTGGCTACCCGCGGCACATCCCAAGAGTGGTATTGAAGCAGCTGGGCGTCAGTTTCGGTACCA
TGTTTTTGCTAAGGTGATGCGGGAAAATCGTGCAACGGCAGTTTTGACTGCGCACCACGCCAATGATCAAGTCGAAACGT
ATCTGATGAAGCTTGCGCGTGGTGGCGACATCTCACAATTGACAGGAATCGCAACTAGTCGGCCCTTTGCAACGGGCCGA
TTAATTCGGCCGTTGTTGACGTGGTCGAAAGATCAGCTACGAAACTATGCTGCCGAGCAGCATGTCGTTTATTTTGAAGA
CGTTACTAATCAAGATGTGGCCTTGACCCGTAATCGGATTCGGCACCGTGTCGTGCCAGAACTTATGACGGTTAATCCCC
AGTTATTAAAGCATGTGGCCGATTACCAGCAACAGTTGACAACGCTGTTGACGGCTAAAAAGCAAATGGTCACGGTACTA
CTGTCGCAGGTCGTAACGGCCACAGGGGCGTTGGTTGTTGATCAGTGGGGCGCACTCCCGAGCCAGTGGCAGTTAGCCGT
TTTTGCGACGTGGCTCGAGCAGCAGACGCAACAATTATTCACCGAGTCGAAGTTACAACCGCTGGTCTCGTGGGGACAAC
GGACGCGGCCTGCCACGAGTAGATTAACGGTAAACGCGGCCTGGGAGTTGTACCGTAACGCTGGTATCATTGAAGCTTTG
CCAATAAAAAAAAGGGGTAAAAAGTTAATGCCCCGTGAAAAAATTATGGTAGACTTAAACCAATGGCAGAAAATTACTGC
AACCCAAACGGTGGGAATCTTTACGCGGGTGCCAAATGTTGTCAGTCAGCCATTTTGGTTAACAGAAGCCGATTGGCCGC
TAGTGTGGCGGCCGTGGCAAGCTGGTGATCGAATCGTTCTCAAGGGCGGTGGTCATCAGCTGGTACGTCGACTGCTGATT
GATCGAAAAGTTCCGGCTGAACGGCGTGAACAGGTGCAAGTGTTAGTGAATGCTCAAGGGAACGTTCTGTGGGTGGTCGG
TCATAAGTTTAGCTATCGTACGACTGGTACCCAGACAGTATTTTTAGCCTTAAAACACGAGAGTTGA

Upstream 100 bases:

>100_bases
TAATGTTCAAATAGGTGACAAACGACCAGCCAACTAATCGGCAAGTTTTAACAATAAATAAATCCAATGTATTTATAATG
AAGTTAGATGGGAGGTTAAG

Downstream 100 bases:

>100_bases
AAGGAGTACATCTGAAACGATGAACAACGACATTGAACGAGTACTTTATAGTCGCGAGGATATCCATCAGGTTGCACAAA
AATTAGGAAAAGAACTCACA

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MTPIQKFNRQLAAAKLLSPQQTVVVAVSTGVDSMVLLHLLQRLPAAERPRVVVAHVNHHLREQSQMEADYLRQYCQQQNL
KLVMADWLPAAHPKSGIEAAGRQFRYHVFAKVMRENRATAVLTAHHANDQVETYLMKLARGGDISQLTGIATSRPFATGR
LIRPLLTWSKDQLRNYAAEQHVVYFEDVTNQDVALTRNRIRHRVVPELMTVNPQLLKHVADYQQQLTTLLTAKKQMVTVL
LSQVVTATGALVVDQWGALPSQWQLAVFATWLEQQTQQLFTESKLQPLVSWGQRTRPATSRLTVNAAWELYRNAGIIEAL
PIKKRGKKLMPREKIMVDLNQWQKITATQTVGIFTRVPNVVSQPFWLTEADWPLVWRPWQAGDRIVLKGGGHQLVRRLLI
DRKVPAERREQVQVLVNAQGNVLWVVGHKFSYRTTGTQTVFLALKHES

Sequences:

>Translated_448_residues
MTPIQKFNRQLAAAKLLSPQQTVVVAVSTGVDSMVLLHLLQRLPAAERPRVVVAHVNHHLREQSQMEADYLRQYCQQQNL
KLVMADWLPAAHPKSGIEAAGRQFRYHVFAKVMRENRATAVLTAHHANDQVETYLMKLARGGDISQLTGIATSRPFATGR
LIRPLLTWSKDQLRNYAAEQHVVYFEDVTNQDVALTRNRIRHRVVPELMTVNPQLLKHVADYQQQLTTLLTAKKQMVTVL
LSQVVTATGALVVDQWGALPSQWQLAVFATWLEQQTQQLFTESKLQPLVSWGQRTRPATSRLTVNAAWELYRNAGIIEAL
PIKKRGKKLMPREKIMVDLNQWQKITATQTVGIFTRVPNVVSQPFWLTEADWPLVWRPWQAGDRIVLKGGGHQLVRRLLI
DRKVPAERREQVQVLVNAQGNVLWVVGHKFSYRTTGTQTVFLALKHES
>Mature_447_residues
TPIQKFNRQLAAAKLLSPQQTVVVAVSTGVDSMVLLHLLQRLPAAERPRVVVAHVNHHLREQSQMEADYLRQYCQQQNLK
LVMADWLPAAHPKSGIEAAGRQFRYHVFAKVMRENRATAVLTAHHANDQVETYLMKLARGGDISQLTGIATSRPFATGRL
IRPLLTWSKDQLRNYAAEQHVVYFEDVTNQDVALTRNRIRHRVVPELMTVNPQLLKHVADYQQQLTTLLTAKKQMVTVLL
SQVVTATGALVVDQWGALPSQWQLAVFATWLEQQTQQLFTESKLQPLVSWGQRTRPATSRLTVNAAWELYRNAGIIEALP
IKKRGKKLMPREKIMVDLNQWQKITATQTVGIFTRVPNVVSQPFWLTEADWPLVWRPWQAGDRIVLKGGGHQLVRRLLID
RKVPAERREQVQVLVNAQGNVLWVVGHKFSYRTTGTQTVFLALKHES

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=216, Percent_Identity=35.6481481481481, Blast_Score=111, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_LACPL (Q88Z33)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784321.1
- ProteinModelPortal:   Q88Z33
- SMR:   Q88Z33
- GeneID:   1061899
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0545
- NMPDR:   fig|220668.1.peg.455
- HOGENOM:   HBG515019
- OMA:   HANDQVE
- ProtClustDB:   CLSK821200
- BioCyc:   LPLA220668:LP_0545-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 51019; Mature: 50888

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIQKFNRQLAAAKLLSPQQTVVVAVSTGVDSMVLLHLLQRLPAAERPRVVVAHVNHHL
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHH
REQSQMEADYLRQYCQQQNLKLVMADWLPAAHPKSGIEAAGRQFRYHVFAKVMRENRATA
HHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VLTAHHANDQVETYLMKLARGGDISQLTGIATSRPFATGRLIRPLLTWSKDQLRNYAAEQ
EEEEECCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHC
HVVYFEDVTNQDVALTRNRIRHRVVPELMTVNPQLLKHVADYQQQLTTLLTAKKQMVTVL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQVVTATGALVVDQWGALPSQWQLAVFATWLEQQTQQLFTESKLQPLVSWGQRTRPATS
HHHHHHHCCHHEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RLTVNAAWELYRNAGIIEALPIKKRGKKLMPREKIMVDLNQWQKITATQTVGIFTRVPNV
EEEEHHHHHHHHCCCCEEECCCHHCCCCCCCHHHHEEEHHHHHEEHHHHHHHHHHHCCHH
VSQPFWLTEADWPLVWRPWQAGDRIVLKGGGHQLVRRLLIDRKVPAERREQVQVLVNAQG
HCCCCEEECCCCCEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHEEEEEECCC
NVLWVVGHKFSYRTTGTQTVFLALKHES
CEEEEEECCEEEECCCCEEEEEEEECCC
>Mature Secondary Structure 
TPIQKFNRQLAAAKLLSPQQTVVVAVSTGVDSMVLLHLLQRLPAAERPRVVVAHVNHHL
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHH
REQSQMEADYLRQYCQQQNLKLVMADWLPAAHPKSGIEAAGRQFRYHVFAKVMRENRATA
HHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
VLTAHHANDQVETYLMKLARGGDISQLTGIATSRPFATGRLIRPLLTWSKDQLRNYAAEQ
EEEEECCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHC
HVVYFEDVTNQDVALTRNRIRHRVVPELMTVNPQLLKHVADYQQQLTTLLTAKKQMVTVL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQVVTATGALVVDQWGALPSQWQLAVFATWLEQQTQQLFTESKLQPLVSWGQRTRPATS
HHHHHHHCCHHEEHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RLTVNAAWELYRNAGIIEALPIKKRGKKLMPREKIMVDLNQWQKITATQTVGIFTRVPNV
EEEEHHHHHHHHCCCCEEECCCHHCCCCCCCHHHHEEEHHHHHEEHHHHHHHHHHHCCHH
VSQPFWLTEADWPLVWRPWQAGDRIVLKGGGHQLVRRLLIDRKVPAERREQVQVLVNAQG
HCCCCEEECCCCCEEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHEEEEEECCC
NVLWVVGHKFSYRTTGTQTVFLALKHES
CEEEEEECCEEEECCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566