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The map label for this gene is spoT [H]

Identifier: 28199582

GI number: 28199582

Start: 1990140

End: 1992347

Strand: Reverse

Name: spoT [H]

Synonym: PD1707

Alternate gene names: 28199582

Gene position: 1992347-1990140 (Counterclockwise)

Preceding gene: 28199586

Following gene: 28199581

Centisome position: 79.07

GC content: 51.36

Gene sequence:

>2208_bases
TTGCTGTTGCTCATAGGGTGGCATAGTCTTCCGAATATGACCCATGTTTCCTTGATCCAGACAGATCACGCCGCACTCCC
GTTGGCTGATACGAAGTTACCTGATTACTTCTTGCAGCTGGAGCGCATGGCTGCTCATTTAACACATGAGCAATTGATGG
CGCTGCGTCGCGCCTGGGTGATGGGTATGGCTGCGCATGCTGGGCAGACACGTAAATCTGGTGAGCCGTATATCACCCAT
CCTGTGGCTGTGGCTTGTGTATTGGCCGACTTAGGTATGGATGTAGAGACATTAATTGCGGCGATCCTTCACGACACCAT
TGAGGACACCTCAGTGACCCGTGAAATACTCGCTGCCGAGTTTGGCGAGACCGTTGCGGATTTGGTGGATGGTGTCACTA
AGTTAGACAAACTGAAGTTCCGGAATCGTCAGGAAGCAGCCGCTGAGAGCTTCCGTAAAATGCTGTTAGCGATGTCGCGT
GATTTGCGTGTGATCATGATCAAATTGGCTGATCGCTTGCACAATATGCGCACCCTTGGCGCGCAGAGTATTGAGGCGCG
TAGCCGCATTGCACGTGAGACGCTGGAGATTTACGCGCCGATTGCTCAGCGTCTTGGCATGGCACTGATGAAATCCGAGC
TTCAAAACCTTGGATTTCGTGCATTGCATCCGTGGCGCCACGCCATCATTGAGAAACATATTCTCAACCAACCCGTGGTG
CGCCGTGAGGCGATGGAACGCGTTGAATTGCAGTTGTCCGGGCGCTTGATACAAGAGGGAGTGCAGCACCGTGTAGTGAG
TCGGATCAAGACTCCGTGGAGCATCTACAACAAGATGCACGACGATAACAAATCTTTCGACCAAGTGATGGATGTCTTCG
GATTTCGATTGGTTGTTAAGAGCGTTGCTGATTGTTACCACGCATTGGGTATCGTGCATGGCACTTTAAAACCTTTAGAT
GGACGGTTCCGGGATTTCATCGCGATTCCCAAAACAAATGGCTACCAGTCGCTGCATACTGTTCTATTTGGCCCATACGG
CTCCCCGATCGAAGTGCAGATTCGTACCGAAGAGATGGATATGATTGCTGAACGCGGCATTGCCGCACACTGGGCCTACA
AGTGTGGCAGCGATTCGCCGAATAGCGCGCAGAGCCGCGTTCATGCCTGGATTGCCGAATTGCTCGAATCACAGCGCACC
GCGATTTCCTCAATGGAATTCTTGGATAACTTCAAGGTTGATCTATTTCCAGATGAGATCTATTTATTCACGCCCAAGGG
CAAGATCTTTGCTTTGCCAAAAAATTCCACTGCTCTTGATTTCGCTTATGCGGTACATACCGATATTGGCAATCATGCCG
TGACCTCTCGGGTGGATAAACAGCTGATGCCACTGCGTACCAAACTCTCTAGTGGACAAACTGTGGAAATCATCACGGCA
CGCTCTGCCTTACCCAAACCGCAATGGTTGGAATTCGTAGTCAGCAGCAAGGCACGTACTGCAATCCGCCATCAACTCAA
ACAGCTTCAACACGAGGATGCCGTACAACTGGGTCACCGAATGCTTGATCGTGCGTTGGAAGCCATGGACAGCTCGTTGG
AACGTCTGCCAAAGGGCCGACTGGATGCCTTCCTGGCTGAACATCACTTTCAGCGCTTAGAGGCCTTGCTTGCTGACATC
GCCTTGGGTAACTGGATGCCTAATCAGACTGCACAAGCATTGCTGACTTACACGGAATTGCTTGGCAACACGCCGATTGA
GCACTCACGCGAGAAAATTTTGATCAATGGTAGCGAGCGTGGCGTCATCAGCTTTGCAAACTGTTGTCAACCAATTCCTG
GTGATGATGTGATGGGCTACCACACCACTGGAAAGGGGATTGTTGTCCATCGCCTGGATTGTCCAAACCTGAATGAATTG
CGTAAATCACCTGAGCGTTGGGTGCCGATCGATTGGGATTTAAACGTTTCTGGTGATTACGATGCCTCGTTACTCGTCGA
AGCTGAGAATCGCACTGGCGTACTGGCCCAATTAGCTGCGGCGATCGCGCAGAGCCATTCCAATATTGAACATGTTGACT
ACCTGGAGCGCGACTTTAATGCAGCCCTACTGCGCTTCAGTATCCAGGTGCGTGACCGCCGTCACCTAGCCGAAGTGATG
CGCCGGTTACGCCGCTTGCCTGCAGTACAGAGCGTATATCGTCAATAG

Upstream 100 bases:

>100_bases
CCTGATGCGGGGAACCATGAACTGTGAAGTGATCCGCGCATCTCTATGATTGTGAACCTGGATAGGCTTTATCGTCGCGG
CCTGTCGCATTAGATGCTAT

Downstream 100 bases:

>100_bases
CTTCCGTCCTTGATACCAGCGTTTTGACTAAGGTATTAGTAGATGAGACCTGCACCTCATGCACTCTTTCTCCACTGGCT
TTCTTCTGCAACTGGAGCAT

Product: pentaphosphate guanosine-3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MLLLIGWHSLPNMTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRKSGEPYITH
PVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSR
DLRVIMIKLADRLHNMRTLGAQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVV
RREAMERVELQLSGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIVHGTLKPLD
GRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRT
AISSMEFLDNFKVDLFPDEIYLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITA
RSALPKPQWLEFVVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQRLEALLADI
ALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNEL
RKSPERWVPIDWDLNVSGDYDASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVM
RRLRRLPAVQSVYRQ

Sequences:

>Translated_735_residues
MLLLIGWHSLPNMTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRKSGEPYITH
PVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSR
DLRVIMIKLADRLHNMRTLGAQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVV
RREAMERVELQLSGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIVHGTLKPLD
GRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRT
AISSMEFLDNFKVDLFPDEIYLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITA
RSALPKPQWLEFVVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQRLEALLADI
ALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNEL
RKSPERWVPIDWDLNVSGDYDASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVM
RRLRRLPAVQSVYRQ
>Mature_735_residues
MLLLIGWHSLPNMTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWVMGMAAHAGQTRKSGEPYITH
PVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAEFGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSR
DLRVIMIKLADRLHNMRTLGAQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVV
RREAMERVELQLSGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVKSVADCYHALGIVHGTLKPLD
GRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMDMIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRT
AISSMEFLDNFKVDLFPDEIYLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITA
RSALPKPQWLEFVVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGRLDAFLAEHHFQRLEALLADI
ALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSERGVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNEL
RKSPERWVPIDWDLNVSGDYDASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVM
RRLRRLPAVQSVYRQ

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=692, Percent_Identity=47.6878612716763, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=675, Percent_Identity=33.4814814814815, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 83040; Mature: 83040

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLIGWHSLPNMTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWV
CEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGMAAHAGQTRKSGEPYITHPVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAE
HHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
AQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCHH
RREAMERVELQLSGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SVADCYHALGIVHGTLKPLDGRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMD
HHHHHHHHHHHHHCCCCCCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHH
MIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFKVDLFPDEI
HHHHCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
YLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITA
EEECCCCCEEEECCCCCCEEEHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
RSALPKPQWLEFVVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LDAFLAEHHFQRLEALLADIALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSER
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
GVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDWDLNVSGDY
CEEEHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHCCCCCCCCCEEEECCCCCC
DASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVM
CCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLRRLPAVQSVYRQ
HHHHHCHHHHHHHCC
>Mature Secondary Structure
MLLLIGWHSLPNMTHVSLIQTDHAALPLADTKLPDYFLQLERMAAHLTHEQLMALRRAWV
CEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGMAAHAGQTRKSGEPYITHPVAVACVLADLGMDVETLIAAILHDTIEDTSVTREILAAE
HHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FGETVADLVDGVTKLDKLKFRNRQEAAAESFRKMLLAMSRDLRVIMIKLADRLHNMRTLG
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
AQSIEARSRIARETLEIYAPIAQRLGMALMKSELQNLGFRALHPWRHAIIEKHILNQPVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCCHH
RREAMERVELQLSGRLIQEGVQHRVVSRIKTPWSIYNKMHDDNKSFDQVMDVFGFRLVVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SVADCYHALGIVHGTLKPLDGRFRDFIAIPKTNGYQSLHTVLFGPYGSPIEVQIRTEEMD
HHHHHHHHHHHHHCCCCCCCCCCHHEEECCCCCCHHHHHHHHCCCCCCCEEEEEEHHHHH
MIAERGIAAHWAYKCGSDSPNSAQSRVHAWIAELLESQRTAISSMEFLDNFKVDLFPDEI
HHHHCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEE
YLFTPKGKIFALPKNSTALDFAYAVHTDIGNHAVTSRVDKQLMPLRTKLSSGQTVEIITA
EEECCCCCEEEECCCCCCEEEHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
RSALPKPQWLEFVVSSKARTAIRHQLKQLQHEDAVQLGHRMLDRALEAMDSSLERLPKGR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LDAFLAEHHFQRLEALLADIALGNWMPNQTAQALLTYTELLGNTPIEHSREKILINGSER
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
GVISFANCCQPIPGDDVMGYHTTGKGIVVHRLDCPNLNELRKSPERWVPIDWDLNVSGDY
CEEEHHHHCCCCCCCCCCCEEECCCCEEEEECCCCCHHHHHCCCCCCCCCEEEECCCCCC
DASLLVEAENRTGVLAQLAAAIAQSHSNIEHVDYLERDFNAALLRFSIQVRDRRHLAEVM
CCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRLRRLPAVQSVYRQ
HHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]