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The map label for this gene is recG [H]

Identifier: 28199580

GI number: 28199580

Start: 1987389

End: 1989545

Strand: Reverse

Name: recG [H]

Synonym: PD1705

Alternate gene names: 28199580

Gene position: 1989545-1987389 (Counterclockwise)

Preceding gene: 28199581

Following gene: 28199579

Centisome position: 78.96

GC content: 56.28

Gene sequence:

>2157_bases
ATGCCGCGTGCACGTTCGCATGCCATCGACTTGAACGTAGCTGGCAATGCCCCATTGTCGGTGCTGCCGCGGGTTGGGCC
GGTATTAGCTGGAAAATGCGCTGCACGTGGCATCTTCACCTTGCAGGATTTGTGGCTACTGCTCCCATTACGTTATGAGG
ACCGTACCCGGCTAACCCCGATTGCCGAGCTTCAGGATGGGGTTGCGGTTCAGATTGAGGCCCGAGTGGAAGCGGTGGAG
CGTAGTTTCCGTTACCGGCCGCTATTACGCGTGGTGTTGTCCGACGACGCACATCACATACTGACGATGCGTTTTTTCCA
TTTTCGCGCCGCACAGATTGCACGGTTTACTGTCGGCACACGGGTGCGTGCGTATGGCGTACCCAAACTTGGCCAATATG
GTTGGGAGATTGTCCATCCCAGCTACCGCATTCTTGCCCCTGGTGAAGCTGTCAGCCTCAATGACTGCTTGGACCCTGTG
TATCCGGTGATTGATGGCGTGGGGCCTGCCATCGTGCGTCAGCTCATCCGCGAAGCGTTGGAACATTTACCTACGGATGC
AGCCTTAGAACTCTTGCCAGACGTTTGGTTGAGTGACCTTGGCCTGCCAACGCTGCGTACAGCCCTACTTATCATGCACC
GGCCCCCGCTGGATGCGGATATTGCCCGATTGATGGTGGGCACTCACCCCGCGCAGCAGCGCTTGTCGTTGGAAGAATTG
CTTTCCCACCAACTCAGCCTGCGTCGCCAGCGCATTGCATTGCAACGTCATTCCGCCCCCGCATTGCCTGATGGAGCGGC
TTTGGTGGCCTCTTTGTTGCACGCACTGCCGTTTCACCTCACTGCTGCGCAGCAACGGGTATTCGCCCAGATTGCTGCTG
ATGTGGCCAAGCCGGTCCCAATGCTGCGTCTTGTGCAGGGTGATGTTGGCAGCGGTAAGACCGTGGTTGCAGCATTGGCC
GCATTACTTGCTGTTGAGCAAAACAAGCAGGTGGCTCTGGCGGCACCGACAGCGTTACTGGCCGAGCAACACTTTATCAA
CCTGCGTGACTGGCTGGAGCCATTGGGCGTGCGTGTGTATTGGTTGGCTGCCAAGGTGACCGGCAAGGCCCGTCTCCAAG
CGCTGAATGATGTGGCCTCTGGTGATGCGCAGGTGGTCGTCGGTACACATGCCCTGATGCAAGAGTCTGTGGTGTTTCAT
GACTTGGCACTGACCATTGTCGATGAACAGCACCGTTTTGGCGTCCATCAGCGCTTGTTGCTTCGTGACAAAGGGGCTAC
TGATGGCATTGTGCCGCATCAGTTAGTGATGACCGCTACACCGATTCCACGCACCTTAGCGATGGCTGCGTATGCTGATT
TGGATGTTTCGGTGATTGACGAAATGCCGCCTGGCCGTACCCCGGTGCAAACGAATGTATTGAGTGCTGAGCGTCGCCCG
GAATTGGTGGAACGTATCCGTCTGGCTTGTGCTCAGGGGCGCCAGGTTTATTGGGTGTGTACGCTGATCGACGAAAGCCA
AACGGAAGCAGAACAGGCACCCCCCAGCAGTAACGATATTGGGCATCGCAGCGAGGTACAGGCGGCACAGGCCACCTATG
AAGCACTGTCTGCACAATTGCCTGGTGTCCGTGTCGGACTCGTGCATGGGCGCATGAAGGCAGCGGAAAAACAGCGGACA
ATGCGTGCGTTCAAATGCAACGAAATTGATGTCTTAGTTGCTACCACGGTGATTGAGGTGGGTGTCGATGTCCCCAATGC
GTCGCTCATGATTATCGAAAATGCTGAGCGTTTGGGTTTAGCGCAACTCCATCAGTTACGTGGTCGGGTCGGACGTGGTT
CTGTTGTGTCAAGTTGCGTTCTGTTGTATCAGCCCCCACTGTCGATGCTGGCGCGTCAGCGTCTGCAAATCATGCGCCAG
ACGAACGATGGTTTTGCGATTGCTGAAAAAGATCTGGAATTACGTGGTCCCGGCGAATTGTTGGGGACACGTCAAACAGG
TCTTGCAGCGTTTCGTGTTGCTGACCTTGCCCGCGATGCCCATCTGCTGCCCCGCGTGTATTCGTTGGCGAACATGTTGC
TTGACGAATCGCCACAGTTAGCTGATCAAGTGATTGCGCGCTGGATTGGTAGTGCGGTGCGTTATGCATCCGCCTGA

Upstream 100 bases:

>100_bases
AGGCCCCCTTCCCAGCACGCTCCACCATTCAGGTCTCCGGTTTACCCAAGGGCGCGGATTTCGAGGTGGATGCAGTGATG
GTGATTGACTGATCCCTGCT

Downstream 100 bases:

>100_bases
TGCCTGTTATCTATTGCACGTATCACTGATCTCTCTGTCCCAGAGGATACGTTCGTTGCTTTTAGCAACGTTGAGTGTCG
CCTGGTACCGCACTCAATGC

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MPRARSHAIDLNVAGNAPLSVLPRVGPVLAGKCAARGIFTLQDLWLLLPLRYEDRTRLTPIAELQDGVAVQIEARVEAVE
RSFRYRPLLRVVLSDDAHHILTMRFFHFRAAQIARFTVGTRVRAYGVPKLGQYGWEIVHPSYRILAPGEAVSLNDCLDPV
YPVIDGVGPAIVRQLIREALEHLPTDAALELLPDVWLSDLGLPTLRTALLIMHRPPLDADIARLMVGTHPAQQRLSLEEL
LSHQLSLRRQRIALQRHSAPALPDGAALVASLLHALPFHLTAAQQRVFAQIAADVAKPVPMLRLVQGDVGSGKTVVAALA
ALLAVEQNKQVALAAPTALLAEQHFINLRDWLEPLGVRVYWLAAKVTGKARLQALNDVASGDAQVVVGTHALMQESVVFH
DLALTIVDEQHRFGVHQRLLLRDKGATDGIVPHQLVMTATPIPRTLAMAAYADLDVSVIDEMPPGRTPVQTNVLSAERRP
ELVERIRLACAQGRQVYWVCTLIDESQTEAEQAPPSSNDIGHRSEVQAAQATYEALSAQLPGVRVGLVHGRMKAAEKQRT
MRAFKCNEIDVLVATTVIEVGVDVPNASLMIIENAERLGLAQLHQLRGRVGRGSVVSSCVLLYQPPLSMLARQRLQIMRQ
TNDGFAIAEKDLELRGPGELLGTRQTGLAAFRVADLARDAHLLPRVYSLANMLLDESPQLADQVIARWIGSAVRYASA

Sequences:

>Translated_718_residues
MPRARSHAIDLNVAGNAPLSVLPRVGPVLAGKCAARGIFTLQDLWLLLPLRYEDRTRLTPIAELQDGVAVQIEARVEAVE
RSFRYRPLLRVVLSDDAHHILTMRFFHFRAAQIARFTVGTRVRAYGVPKLGQYGWEIVHPSYRILAPGEAVSLNDCLDPV
YPVIDGVGPAIVRQLIREALEHLPTDAALELLPDVWLSDLGLPTLRTALLIMHRPPLDADIARLMVGTHPAQQRLSLEEL
LSHQLSLRRQRIALQRHSAPALPDGAALVASLLHALPFHLTAAQQRVFAQIAADVAKPVPMLRLVQGDVGSGKTVVAALA
ALLAVEQNKQVALAAPTALLAEQHFINLRDWLEPLGVRVYWLAAKVTGKARLQALNDVASGDAQVVVGTHALMQESVVFH
DLALTIVDEQHRFGVHQRLLLRDKGATDGIVPHQLVMTATPIPRTLAMAAYADLDVSVIDEMPPGRTPVQTNVLSAERRP
ELVERIRLACAQGRQVYWVCTLIDESQTEAEQAPPSSNDIGHRSEVQAAQATYEALSAQLPGVRVGLVHGRMKAAEKQRT
MRAFKCNEIDVLVATTVIEVGVDVPNASLMIIENAERLGLAQLHQLRGRVGRGSVVSSCVLLYQPPLSMLARQRLQIMRQ
TNDGFAIAEKDLELRGPGELLGTRQTGLAAFRVADLARDAHLLPRVYSLANMLLDESPQLADQVIARWIGSAVRYASA
>Mature_717_residues
PRARSHAIDLNVAGNAPLSVLPRVGPVLAGKCAARGIFTLQDLWLLLPLRYEDRTRLTPIAELQDGVAVQIEARVEAVER
SFRYRPLLRVVLSDDAHHILTMRFFHFRAAQIARFTVGTRVRAYGVPKLGQYGWEIVHPSYRILAPGEAVSLNDCLDPVY
PVIDGVGPAIVRQLIREALEHLPTDAALELLPDVWLSDLGLPTLRTALLIMHRPPLDADIARLMVGTHPAQQRLSLEELL
SHQLSLRRQRIALQRHSAPALPDGAALVASLLHALPFHLTAAQQRVFAQIAADVAKPVPMLRLVQGDVGSGKTVVAALAA
LLAVEQNKQVALAAPTALLAEQHFINLRDWLEPLGVRVYWLAAKVTGKARLQALNDVASGDAQVVVGTHALMQESVVFHD
LALTIVDEQHRFGVHQRLLLRDKGATDGIVPHQLVMTATPIPRTLAMAAYADLDVSVIDEMPPGRTPVQTNVLSAERRPE
LVERIRLACAQGRQVYWVCTLIDESQTEAEQAPPSSNDIGHRSEVQAAQATYEALSAQLPGVRVGLVHGRMKAAEKQRTM
RAFKCNEIDVLVATTVIEVGVDVPNASLMIIENAERLGLAQLHQLRGRVGRGSVVSSCVLLYQPPLSMLARQRLQIMRQT
NDGFAIAEKDLELRGPGELLGTRQTGLAAFRVADLARDAHLLPRVYSLANMLLDESPQLADQVIARWIGSAVRYASA

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=702, Percent_Identity=56.2678062678063, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=398, Percent_Identity=39.9497487437186, Blast_Score=256, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78626; Mature: 78495

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRARSHAIDLNVAGNAPLSVLPRVGPVLAGKCAARGIFTLQDLWLLLPLRYEDRTRLTP
CCCCCCCEEEEEECCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
IAELQDGVAVQIEARVEAVERSFRYRPLLRVVLSDDAHHILTMRFFHFRAAQIARFTVGT
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RVRAYGVPKLGQYGWEIVHPSYRILAPGEAVSLNDCLDPVYPVIDGVGPAIVRQLIREAL
EEEECCCCCCCCCCEEEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHLPTDAALELLPDVWLSDLGLPTLRTALLIMHRPPLDADIARLMVGTHPAQQRLSLEEL
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCHHHH
LSHQLSLRRQRIALQRHSAPALPDGAALVASLLHALPFHLTAAQQRVFAQIAADVAKPVP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MLRLVQGDVGSGKTVVAALAALLAVEQNKQVALAAPTALLAEQHFINLRDWLEPLGVRVY
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCEEE
WLAAKVTGKARLQALNDVASGDAQVVVGTHALMQESVVFHDLALTIVDEQHRFGVHQRLL
EEEEECCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHEEEECCHHHHCHHHHHH
LRDKGATDGIVPHQLVMTATPIPRTLAMAAYADLDVSVIDEMPPGRTPVQTNVLSAERRP
HHCCCCCCCCCCCHHEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHCCCCCH
ELVERIRLACAQGRQVYWVCTLIDESQTEAEQAPPSSNDIGHRSEVQAAQATYEALSAQL
HHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PGVRVGLVHGRMKAAEKQRTMRAFKCNEIDVLVATTVIEVGVDVPNASLMIIENAERLGL
CCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCHHCCH
AQLHQLRGRVGRGSVVSSCVLLYQPPLSMLARQRLQIMRQTNDGFAIAEKDLELRGPGEL
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHH
LGTRQTGLAAFRVADLARDAHLLPRVYSLANMLLDESPQLADQVIARWIGSAVRYASA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRARSHAIDLNVAGNAPLSVLPRVGPVLAGKCAARGIFTLQDLWLLLPLRYEDRTRLTP
CCCCCCEEEEEECCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCC
IAELQDGVAVQIEARVEAVERSFRYRPLLRVVLSDDAHHILTMRFFHFRAAQIARFTVGT
HHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RVRAYGVPKLGQYGWEIVHPSYRILAPGEAVSLNDCLDPVYPVIDGVGPAIVRQLIREAL
EEEECCCCCCCCCCEEEECCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EHLPTDAALELLPDVWLSDLGLPTLRTALLIMHRPPLDADIARLMVGTHPAQQRLSLEEL
HHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHCCHHHH
LSHQLSLRRQRIALQRHSAPALPDGAALVASLLHALPFHLTAAQQRVFAQIAADVAKPVP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
MLRLVQGDVGSGKTVVAALAALLAVEQNKQVALAAPTALLAEQHFINLRDWLEPLGVRVY
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCHHHHHHHHCCEEE
WLAAKVTGKARLQALNDVASGDAQVVVGTHALMQESVVFHDLALTIVDEQHRFGVHQRLL
EEEEECCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHEEEECCHHHHCHHHHHH
LRDKGATDGIVPHQLVMTATPIPRTLAMAAYADLDVSVIDEMPPGRTPVQTNVLSAERRP
HHCCCCCCCCCCCHHEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHCCCCCH
ELVERIRLACAQGRQVYWVCTLIDESQTEAEQAPPSSNDIGHRSEVQAAQATYEALSAQL
HHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PGVRVGLVHGRMKAAEKQRTMRAFKCNEIDVLVATTVIEVGVDVPNASLMIIENAERLGL
CCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCCEEEEEECCHHCCH
AQLHQLRGRVGRGSVVSSCVLLYQPPLSMLARQRLQIMRQTNDGFAIAEKDLELRGPGEL
HHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHH
LGTRQTGLAAFRVADLARDAHLLPRVYSLANMLLDESPQLADQVIARWIGSAVRYASA
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]