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The map label for this gene is cssS

Identifier: 255767751

GI number: 255767751

Start: 3386398

End: 3387753

Strand: Direct

Name: cssS

Synonym: BSU33020

Alternate gene names: 255767751

Gene position: 3386398-3387753 (Clockwise)

Preceding gene: 16080354

Following gene: 16080356

Centisome position: 80.33

GC content: 41.37

Gene sequence:

>1356_bases
ATGAAAAACAAGCCGCTCGCGTTTCAGATATGGGTTGTCATATCCGGCATCCTGTTAGCGATATCGATTTTACTGCTTGT
GTTATTTTCAAACACGCTGCGAGATTTTTTCACTAATGAAACGTATACGACGATTGAAAATGAGCAGCATGTTCTGACAG
AGTACCGCCTGCCAGGTTCGATTGAAAGGCGCTATTACAGCGAGGAAGCGACGGCGCCGACAACTGTCCGCTCCGTACAG
CACGTGCTCCTTCCTGAAAATGAAGAGGCTTCTTCAGACAAGGATTTAAGCATTCTGTCATCTTCATTTATCCACAAGGT
GTACAAGCTGGCTGATAAGCAGGAAGCTAAAAAGAAACGTTACAGCGCCGACGTCAATGGAGAGAAAGTGTTTTTTGTCA
TTAAAAAGGGACTTTCCGTCAATGGACAATCAGCGATGATGCTCTCTTACGCGCTTGATTCTTATCGGGACGATTTGGCC
TATACCTTGTTCAAACAGCTTCTGTTTATTATAGCTGTCGTCATTTTATTAAGCTGGATTCCGGCTATTTGGCTTGCAAA
GTATTTATCAAGGCCTCTTGTATCATTTGAAAAACACGTCAAACGGATTTCTGAACAGGATTGGGATGACCCAGTAAAAG
TGGACCGGAAAGATGAAATCGGCAAATTGGGCCATACCATCGAAGAGATGCGCCAAAAGCTTGTGCAAAAGGATGAAACA
GAAAGAACTCTATTGCAAAATATCTCTCATGATTTAAAAACGCCGGTCATGGTCATCAGAGGCTATACACAATCAATTAA
AGACGGGATTTTTCCTAAAGGAGACCTTGAAAACACTGTAGATGTTATTGAATGCGAAGCTCTTAAGCTGGAGAAAAAAA
TAAAGGATTTATTATATTTAACGAAGCTGGATTATTTAGCGAAGCAAAAAGTGCAGCACGACATGTTCAGTATTGTGGAA
GTGACAGAAGAAGTCATCGAACGATTGAAGTGGGCGCGGAAAGAACTATCGTGGGAAATTGATGTAGAAGAGGATATTTT
GATGCCGGGCGATCCGGAGCAATGGAACAAACTCCTCGAAAACATTTTGGAAAATCAAATCCGCTATGCTGAGACAAAAA
TAGAAATCAGCATGAAACAAGATGATCGAAATATCGTGATCACCATTAAAAATGACGGTCCGCATATTGAAGATGAGATG
CTCTCCAGCCTCTATGAGCCTTTTAATAAAGGGAAGAAAGGCGAATTCGGCATTGGTCTAAGCATCGTAAAACGAATTTT
AACTCTTCATAAGGCATCTATCTCAATTGAAAATGACAAAACGGGTGTAACATACCGCATAGCAGTGCCAAAATAG

Upstream 100 bases:

>100_bases
CAGACCGGGTCGTTGATGATCTCGTCCGGAGACTGCGCAGAAAGATGCCTGAATTGAAGGTGGAGACGATTTACGGTTTC
GGCTACAGGATGATGTCATC

Downstream 100 bases:

>100_bases
ACTGTAGATGTTTTGCAGTCTATTTTTTTATGTGAAAGAATAGCCGTTAAAATCTCTGTAATTTGTTTTGAGCGGCTGTT
TGCATTGATGAACCCATTTT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKNKPLAFQIWVVISGILLAISILLLVLFSNTLRDFFTNETYTTIENEQHVLTEYRLPGSIERRYYSEEATAPTTVRSVQ
HVLLPENEEASSDKDLSILSSSFIHKVYKLADKQEAKKKRYSADVNGEKVFFVIKKGLSVNGQSAMMLSYALDSYRDDLA
YTLFKQLLFIIAVVILLSWIPAIWLAKYLSRPLVSFEKHVKRISEQDWDDPVKVDRKDEIGKLGHTIEEMRQKLVQKDET
ERTLLQNISHDLKTPVMVIRGYTQSIKDGIFPKGDLENTVDVIECEALKLEKKIKDLLYLTKLDYLAKQKVQHDMFSIVE
VTEEVIERLKWARKELSWEIDVEEDILMPGDPEQWNKLLENILENQIRYAETKIEISMKQDDRNIVITIKNDGPHIEDEM
LSSLYEPFNKGKKGEFGIGLSIVKRILTLHKASISIENDKTGVTYRIAVPK

Sequences:

>Translated_451_residues
MKNKPLAFQIWVVISGILLAISILLLVLFSNTLRDFFTNETYTTIENEQHVLTEYRLPGSIERRYYSEEATAPTTVRSVQ
HVLLPENEEASSDKDLSILSSSFIHKVYKLADKQEAKKKRYSADVNGEKVFFVIKKGLSVNGQSAMMLSYALDSYRDDLA
YTLFKQLLFIIAVVILLSWIPAIWLAKYLSRPLVSFEKHVKRISEQDWDDPVKVDRKDEIGKLGHTIEEMRQKLVQKDET
ERTLLQNISHDLKTPVMVIRGYTQSIKDGIFPKGDLENTVDVIECEALKLEKKIKDLLYLTKLDYLAKQKVQHDMFSIVE
VTEEVIERLKWARKELSWEIDVEEDILMPGDPEQWNKLLENILENQIRYAETKIEISMKQDDRNIVITIKNDGPHIEDEM
LSSLYEPFNKGKKGEFGIGLSIVKRILTLHKASISIENDKTGVTYRIAVPK
>Mature_451_residues
MKNKPLAFQIWVVISGILLAISILLLVLFSNTLRDFFTNETYTTIENEQHVLTEYRLPGSIERRYYSEEATAPTTVRSVQ
HVLLPENEEASSDKDLSILSSSFIHKVYKLADKQEAKKKRYSADVNGEKVFFVIKKGLSVNGQSAMMLSYALDSYRDDLA
YTLFKQLLFIIAVVILLSWIPAIWLAKYLSRPLVSFEKHVKRISEQDWDDPVKVDRKDEIGKLGHTIEEMRQKLVQKDET
ERTLLQNISHDLKTPVMVIRGYTQSIKDGIFPKGDLENTVDVIECEALKLEKKIKDLLYLTKLDYLAKQKVQHDMFSIVE
VTEEVIERLKWARKELSWEIDVEEDILMPGDPEQWNKLLENILENQIRYAETKIEISMKQDDRNIVITIKNDGPHIEDEM
LSSLYEPFNKGKKGEFGIGLSIVKRILTLHKASISIENDKTGVTYRIAVPK

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1788393, Length=276, Percent_Identity=27.1739130434783, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789808, Length=298, Percent_Identity=25.503355704698, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=25.8771929824561, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1786783, Length=305, Percent_Identity=23.9344262295082, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=24.4813278008299, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787374, Length=285, Percent_Identity=21.4035087719298, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786600, Length=232, Percent_Identity=23.2758620689655, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790436, Length=288, Percent_Identity=21.5277777777778, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790551, Length=285, Percent_Identity=22.8070175438596, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1790861, Length=289, Percent_Identity=21.1072664359862, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082128, Length=298, Percent_Identity=22.8187919463087, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786912, Length=235, Percent_Identity=22.1276595744681, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788279, Length=292, Percent_Identity=22.2602739726027, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CSSS_BACSU (O32193)

Other databases:

- EMBL:   Z93941
- EMBL:   AJ223978
- EMBL:   AL009126
- PIR:   D70045
- RefSeq:   NP_391182.2
- ProteinModelPortal:   O32193
- SMR:   O32193
- EnsemblBacteria:   EBBACT00000001884
- GeneID:   935936
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU33020
- NMPDR:   fig|224308.1.peg.3308
- GenoList:   BSU33020
- GeneTree:   EBGT00050000000010
- HOGENOM:   HBG338303
- ProtClustDB:   CLSK872951
- BioCyc:   BSUB:BSU33020-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00304
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 52098; Mature: 52098

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50885 HAMP; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12617a58)-; HASH(0x11eaf2d0)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKPLAFQIWVVISGILLAISILLLVLFSNTLRDFFTNETYTTIENEQHVLTEYRLPGS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCH
IERRYYSEEATAPTTVRSVQHVLLPENEEASSDKDLSILSSSFIHKVYKLADKQEAKKKR
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YSADVNGEKVFFVIKKGLSVNGQSAMMLSYALDSYRDDLAYTLFKQLLFIIAVVILLSWI
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAIWLAKYLSRPLVSFEKHVKRISEQDWDDPVKVDRKDEIGKLGHTIEEMRQKLVQKDET
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
ERTLLQNISHDLKTPVMVIRGYTQSIKDGIFPKGDLENTVDVIECEALKLEKKIKDLLYL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TKLDYLAKQKVQHDMFSIVEVTEEVIERLKWARKELSWEIDVEEDILMPGDPEQWNKLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHH
NILENQIRYAETKIEISMKQDDRNIVITIKNDGPHIEDEMLSSLYEPFNKGKKGEFGIGL
HHHHHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
SIVKRILTLHKASISIENDKTGVTYRIAVPK
HHHHHHHHHHHHHEEECCCCCCEEEEEECCH
>Mature Secondary Structure
MKNKPLAFQIWVVISGILLAISILLLVLFSNTLRDFFTNETYTTIENEQHVLTEYRLPGS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCH
IERRYYSEEATAPTTVRSVQHVLLPENEEASSDKDLSILSSSFIHKVYKLADKQEAKKKR
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YSADVNGEKVFFVIKKGLSVNGQSAMMLSYALDSYRDDLAYTLFKQLLFIIAVVILLSWI
CCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAIWLAKYLSRPLVSFEKHVKRISEQDWDDPVKVDRKDEIGKLGHTIEEMRQKLVQKDET
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
ERTLLQNISHDLKTPVMVIRGYTQSIKDGIFPKGDLENTVDVIECEALKLEKKIKDLLYL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TKLDYLAKQKVQHDMFSIVEVTEEVIERLKWARKELSWEIDVEEDILMPGDPEQWNKLLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHH
NILENQIRYAETKIEISMKQDDRNIVITIKNDGPHIEDEMLSSLYEPFNKGKKGEFGIGL
HHHHHHHHHHHHEEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCH
SIVKRILTLHKASISIENDKTGVTYRIAVPK
HHHHHHHHHHHHHEEECCCCCCEEEEEECCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295