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The map label for this gene is pbpD

Identifier: 255767716

GI number: 255767716

Start: 3233911

End: 3235785

Strand: Direct

Name: pbpD

Synonym: BSU31490

Alternate gene names: 255767716

Gene position: 3233911-3235785 (Clockwise)

Preceding gene: 50812285

Following gene: 16080202

Centisome position: 76.71

GC content: 43.95

Gene sequence:

>1875_bases
GTGACCATGTTACGAAAAATAATCGGATGGATTTTGCTCCTTTGCATAATCCCATTGTTTGCATTTACAGTTATCGCTTC
CGGAAAAGAAGTAAAACAAATGAAGTCCCTGGATCAGGTGCTTGATAAAAATATTGATCTGAAAGATATCAGCCTTGTTC
AGAACAGCTACATGTATGACAGGGACGGCTCCCTTGTCTCGGAAATCGTCAGCGACCACGAAAACCGTGTGCTCGTTCCA
TTTAACAAGATTCCCGAGGAAGTCAAACAGATTTTCTTAACATCTGAGGACCGCCATTTTTACGAGCATAAAGGCTTTGA
CTTTATGGGAATGGTGCGGGCTACTGCTTCTAACGTAAAAGACAAGAAAATTGACCAGGGTGCCAGCACCATCACACAGC
AGCTTTCAAGAAACTTGTATTTGAGCCACGAACGCTCCTTCAGCCGCAAGCTGACAGAGCTCGCGTATTCCTATCAGCTG
GAGAAAAAATATACGAAAAATGAAATTCTTGAAGCTTACTTAAATACCATTTATTTCAACAACGGTGTTTACGGCGTCGG
TTCAGCAGCTCAATTCTATTTCAGCAAACCGCTGAAATCTCTCACAGTGGGAGAAATGGCTTTTATCTGTGCTATACCTA
ATAACCCTACATTATATGATCCTCTCAAGCATTTTGACTACACGAAAAGCCGTCAAGAACGTTTACTTAAAGGGCTAAAA
GATGCCGGGGTCATTACGGACAAAGAGCTGAAAAAAGCCGTAAAACAAAAAATCAAACTGGATGTTGAAAAAAGAGAAGA
CAAATATCCTGACTACGTTTCCTACGTCAATGATGAATTCACTCAGCTGGTGTCTGAATCAGAAGGCTTTGATAAACGCC
TTCAAAAAGCATCAGGAAAACAAAAAGAAAAGATCGAGAACGAGCTGTCCGCAAGAGTCAGCACGCTGATGAAAGACGGA
GTGAAAATTTATACTGCACTTGATCCGTACATGCAAAATCAAGTTGTGGCACAAATGAATTCCAAGCTCCCGTATGCGGA
CGTACAGGGCGGAGCGGCTGTGATTAATCACCAGACGCATCAAATCATTGCTCTATCCGGCGGGAAAAACTATCAAAAAT
ATGACTTTAACCGCGCCTACCAGGCGTACAGGCAGCCGGGTTCATCCATTAAGCCGCTTCTTGATTACGGCCCTTATATT
GAACAGACCGGCGCGACAACAAGCAGCACCATTGATGCCAGCAAGTTTTGCAGCAAAGACTACTGTCCGCAAAACTACAA
TAACCGCACTTACGGCACAGTGACGCTTGATACGGCGTTTAAAAATTCATATAATACCCCGGCTATTAGAATGCTCGACC
GCGTCGGCATCCAAAAGGCATTCAGTTATATTGAGCCGTATCATTTTGCCAAACTTGTCGACAGTGACTATCTTCTTCCG
GCGGCGCTTGGCGGATTTACAAACGGCATGACGCCTCTTGAGATGACAAAGGCTTATACGACATTCGGAAACAGCGGAAG
CTATACGCCGAGCCATGCGATTACAAAGGTGACAGACCTTAAAGGAAAAACACTTTATAAATGGAATGATAAAGCGACTC
AAATATTCAGCGTCCGCACGAACATGCAGCTGAAAAAACTGATGTCTTCCGTTGTGAAAAGCGGAACAGGGAAAAAAGCA
TATTTCAATGCGCCTTACATCGGCGGTAAAACCGGAACATCAAATGATTATCACGATATGTGGTTTGTCGGCCTGACTGA
TACGTATACAATGGGTGTCTGGGTCGGAAAGGATACACCGACCAGCGTTGAATATCTTCACAGCATCAGCCCTCAGCTTT
CCATCTGGAAAGGAACGCTGCAAGCGGCTTATTAA

Upstream 100 bases:

>100_bases
CAAGCTGATTCCGAGAAAAAACTAAGGAATCTTCTAAATTTAACCTTCTCGTAATCTCAAAAGAATGGTACGATATGGCT
AGAATTTAGGAGAAAAGAGA

Downstream 100 bases:

>100_bases
AGGATCGGAGGGGATATGGAGTGACTTCAGAACAAATTCCAAATCGCGTTTTGCTGTTTGACGGTGTCTGCAATTTATGC
AATGGCGCCGTGCAGTTTAT

Product: penicillin-binding protein 4

Products: NA

Alternate protein names: PBP 4

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MTMLRKIIGWILLLCIIPLFAFTVIASGKEVKQMKSLDQVLDKNIDLKDISLVQNSYMYDRDGSLVSEIVSDHENRVLVP
FNKIPEEVKQIFLTSEDRHFYEHKGFDFMGMVRATASNVKDKKIDQGASTITQQLSRNLYLSHERSFSRKLTELAYSYQL
EKKYTKNEILEAYLNTIYFNNGVYGVGSAAQFYFSKPLKSLTVGEMAFICAIPNNPTLYDPLKHFDYTKSRQERLLKGLK
DAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDG
VKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYI
EQTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIRMLDRVGIQKAFSYIEPYHFAKLVDSDYLLP
AALGGFTNGMTPLEMTKAYTTFGNSGSYTPSHAITKVTDLKGKTLYKWNDKATQIFSVRTNMQLKKLMSSVVKSGTGKKA
YFNAPYIGGKTGTSNDYHDMWFVGLTDTYTMGVWVGKDTPTSVEYLHSISPQLSIWKGTLQAAY

Sequences:

>Translated_624_residues
MTMLRKIIGWILLLCIIPLFAFTVIASGKEVKQMKSLDQVLDKNIDLKDISLVQNSYMYDRDGSLVSEIVSDHENRVLVP
FNKIPEEVKQIFLTSEDRHFYEHKGFDFMGMVRATASNVKDKKIDQGASTITQQLSRNLYLSHERSFSRKLTELAYSYQL
EKKYTKNEILEAYLNTIYFNNGVYGVGSAAQFYFSKPLKSLTVGEMAFICAIPNNPTLYDPLKHFDYTKSRQERLLKGLK
DAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDG
VKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYI
EQTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIRMLDRVGIQKAFSYIEPYHFAKLVDSDYLLP
AALGGFTNGMTPLEMTKAYTTFGNSGSYTPSHAITKVTDLKGKTLYKWNDKATQIFSVRTNMQLKKLMSSVVKSGTGKKA
YFNAPYIGGKTGTSNDYHDMWFVGLTDTYTMGVWVGKDTPTSVEYLHSISPQLSIWKGTLQAAY
>Mature_623_residues
TMLRKIIGWILLLCIIPLFAFTVIASGKEVKQMKSLDQVLDKNIDLKDISLVQNSYMYDRDGSLVSEIVSDHENRVLVPF
NKIPEEVKQIFLTSEDRHFYEHKGFDFMGMVRATASNVKDKKIDQGASTITQQLSRNLYLSHERSFSRKLTELAYSYQLE
KKYTKNEILEAYLNTIYFNNGVYGVGSAAQFYFSKPLKSLTVGEMAFICAIPNNPTLYDPLKHFDYTKSRQERLLKGLKD
AGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDGV
KIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIE
QTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIRMLDRVGIQKAFSYIEPYHFAKLVDSDYLLPA
ALGGFTNGMTPLEMTKAYTTFGNSGSYTPSHAITKVTDLKGKTLYKWNDKATQIFSVRTNMQLKKLMSSVVKSGTGKKAY
FNAPYIGGKTGTSNDYHDMWFVGLTDTYTMGVWVGKDTPTSVEYLHSISPQLSIWKGTLQAAY

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786343, Length=583, Percent_Identity=28.3018867924528, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI87082258, Length=256, Percent_Identity=35.546875, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788867, Length=526, Percent_Identity=22.6235741444867, Blast_Score=132, Evalue=8e-32,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=36.3636363636364, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PBPD_BACSU (P40750)

Other databases:

- EMBL:   U11882
- EMBL:   Z93933
- EMBL:   AL009126
- PIR:   A55220
- RefSeq:   NP_391027.2
- ProteinModelPortal:   P40750
- SMR:   P40750
- EnsemblBacteria:   EBBACT00000003592
- GeneID:   938851
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31490
- NMPDR:   fig|224308.1.peg.3152
- GenoList:   BSU31490
- GeneTree:   EBGT00070000031846
- HOGENOM:   HBG672432
- PhylomeDB:   P40750
- ProtClustDB:   CLSK887793
- BioCyc:   BSUB:BSU31490-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase; SSF56601 PBP_transp_fold

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 70659; Mature: 70528

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLRKIIGWILLLCIIPLFAFTVIASGKEVKQMKSLDQVLDKNIDLKDISLVQNSYMYD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEC
RDGSLVSEIVSDHENRVLVPFNKIPEEVKQIFLTSEDRHFYEHKGFDFMGMVRATASNVK
CCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCC
DKKIDQGASTITQQLSRNLYLSHERSFSRKLTELAYSYQLEKKYTKNEILEAYLNTIYFN
HHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
NGVYGVGSAAQFYFSKPLKSLTVGEMAFICAIPNNPTLYDPLKHFDYTKSRQERLLKGLK
CCEECCCCHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHH
DAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGK
HCCCCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCH
QKEKIENELSARVSTLMKDGVKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTH
HHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCE
QIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIEQTGATTSSTIDASKFCSKD
EEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHCCCCCCCCCCHHHHHCCC
YCPQNYNNRTYGTVTLDTAFKNSYNTPAIRMLDRVGIQKAFSYIEPYHFAKLVDSDYLLP
CCCCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
AALGGFTNGMTPLEMTKAYTTFGNSGSYTPSHAITKVTDLKGKTLYKWNDKATQIFSVRT
HHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEECC
NMQLKKLMSSVVKSGTGKKAYFNAPYIGGKTGTSNDYHDMWFVGLTDTYTMGVWVGKDTP
CHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCC
TSVEYLHSISPQLSIWKGTLQAAY
HHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TMLRKIIGWILLLCIIPLFAFTVIASGKEVKQMKSLDQVLDKNIDLKDISLVQNSYMYD
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEC
RDGSLVSEIVSDHENRVLVPFNKIPEEVKQIFLTSEDRHFYEHKGFDFMGMVRATASNVK
CCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHCCC
DKKIDQGASTITQQLSRNLYLSHERSFSRKLTELAYSYQLEKKYTKNEILEAYLNTIYFN
HHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEC
NGVYGVGSAAQFYFSKPLKSLTVGEMAFICAIPNNPTLYDPLKHFDYTKSRQERLLKGLK
CCEECCCCHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHH
DAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGK
HCCCCCHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCH
QKEKIENELSARVSTLMKDGVKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTH
HHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCE
QIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIEQTGATTSSTIDASKFCSKD
EEEEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHCCCCCCCCCCHHHHHCCC
YCPQNYNNRTYGTVTLDTAFKNSYNTPAIRMLDRVGIQKAFSYIEPYHFAKLVDSDYLLP
CCCCCCCCCEEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHH
AALGGFTNGMTPLEMTKAYTTFGNSGSYTPSHAITKVTDLKGKTLYKWNDKATQIFSVRT
HHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCEEEEECCCCEEEEEECC
NMQLKKLMSSVVKSGTGKKAYFNAPYIGGKTGTSNDYHDMWFVGLTDTYTMGVWVGKDTP
CHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCC
TSVEYLHSISPQLSIWKGTLQAAY
HHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377