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The map label for this gene is kinB

Identifier: 255767715

GI number: 255767715

Start: 3230067

End: 3231353

Strand: Direct

Name: kinB

Synonym: BSU31450

Alternate gene names: 255767715

Gene position: 3230067-3231353 (Clockwise)

Preceding gene: 16080196

Following gene: 16080198

Centisome position: 76.62

GC content: 43.36

Gene sequence:

>1287_bases
ATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGG
AAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTA
TTTTCCCGATTCATGAAATGGACTACATACAATACGGCCTTCAGATGATTCCCGTTATCATTTGTTTATTTTACATTAGT
ACCGCTTCCGGACTTACAGTCGCCGCATCTGTCCTATGTTTTGAACTGCTTTTTTACGAACCTTCCGCAATGTTTGTTTT
CACTTTGCTGCCTTTTCTTATCATTATCCCAATCTTATTTCAGAAAAAATGGCCATTCATGTCCAAAGCGAAAAAACTGC
TGCTGTCTTTATTGATCAGCTGCGTGGAGATTTTCCTATTTTTCGCCTCAAGCTGGATTCTTTCCGCCTTGAATATTTTA
AATTTTCAAAAATCAGGAATTTTCGTTTACGAGGCGGCAGTCTCAGGGCTTTTCCGCTCCAGTGTTCTTCTATTAAGCAT
CTATATTATCGAAAGCATCGCCGAAAATATCGCCCTGCGTTCACAGCTTATCCATTCTGAAAAAATGACGATTGTGAGTG
AACTGGCAGCGAGCGTCGCCCATGAGGTCAGGAATCCGCTGACAGTTGTCCGCGGGTTTGTCCAGCTGCTTTTTAATGAC
GAAACCCTACAAAACAAGTCGAGTGCGGATTACAAAAAGCTTGTACTGTCAGAGCTTGACCGGGCGCAAGGCATTATCAC
AAACTATCTCGATATGGCCAAACAGCAATTATACGAAAAAGAGGTTTTTGACCTATCTGCCCTTATCAAAGAGACAAGCT
CCCTTATGGTGTCGTACGCCAATTACAAGTCAGTAACCGTCGAGGCTGAAACAGAACCGGACCTTTTGATATACGGAGAC
GCAACAAAGCTGAAGCAGGCTGTCATCAACCTGATGAAAAACAGTATTGAAGCGGTTCCTCACGGGAAGGGGATGATCCA
CATATCAGCCAAAAGAAACGGCCATACGATCATGATTAACATCACAGACAATGGGGTCGGGATGACAGACCATCAAATGC
AAAAACTCGGCGAACCTTATTATTCCTTAAAAACGAACGGAACGGGACTCGGCCTCACCGTAACCTTTTCCATTATTGAA
CATCATCACGGGACCATTTCATTTAACAGCAGCTTCCAAAAAGGCACTACGGTGACCATTAAGCTTCCCGCTGATCTTCC
TCACTAG

Upstream 100 bases:

>100_bases
TTCATTTGTAAAGGCGTTCTTAATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTA
TACGAAAGGATTTCACACGA

Downstream 100 bases:

>100_bases
CAAATCGATTGGAACTTTAATAGAATAAATGCGAGGTGATGACAGATGAGCACGTTTGAGACAGGCTCTATCGTTAAAGG
ATTTTACAAAACAGGTGTAT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEILKDYLLHICFILFPILLYQVFWLGKPAILVPKINSGLVTLFACGASVLCIIFPIHEMDYIQYGLQMIPVIICLFYIS
TASGLTVAASVLCFELLFYEPSAMFVFTLLPFLIIIPILFQKKWPFMSKAKKLLLSLLISCVEIFLFFASSWILSALNIL
NFQKSGIFVYEAAVSGLFRSSVLLLSIYIIESIAENIALRSQLIHSEKMTIVSELAASVAHEVRNPLTVVRGFVQLLFND
ETLQNKSSADYKKLVLSELDRAQGIITNYLDMAKQQLYEKEVFDLSALIKETSSLMVSYANYKSVTVEAETEPDLLIYGD
ATKLKQAVINLMKNSIEAVPHGKGMIHISAKRNGHTIMINITDNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSIIE
HHHGTISFNSSFQKGTTVTIKLPADLPH

Sequences:

>Translated_428_residues
MEILKDYLLHICFILFPILLYQVFWLGKPAILVPKINSGLVTLFACGASVLCIIFPIHEMDYIQYGLQMIPVIICLFYIS
TASGLTVAASVLCFELLFYEPSAMFVFTLLPFLIIIPILFQKKWPFMSKAKKLLLSLLISCVEIFLFFASSWILSALNIL
NFQKSGIFVYEAAVSGLFRSSVLLLSIYIIESIAENIALRSQLIHSEKMTIVSELAASVAHEVRNPLTVVRGFVQLLFND
ETLQNKSSADYKKLVLSELDRAQGIITNYLDMAKQQLYEKEVFDLSALIKETSSLMVSYANYKSVTVEAETEPDLLIYGD
ATKLKQAVINLMKNSIEAVPHGKGMIHISAKRNGHTIMINITDNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSIIE
HHHGTISFNSSFQKGTTVTIKLPADLPH
>Mature_428_residues
MEILKDYLLHICFILFPILLYQVFWLGKPAILVPKINSGLVTLFACGASVLCIIFPIHEMDYIQYGLQMIPVIICLFYIS
TASGLTVAASVLCFELLFYEPSAMFVFTLLPFLIIIPILFQKKWPFMSKAKKLLLSLLISCVEIFLFFASSWILSALNIL
NFQKSGIFVYEAAVSGLFRSSVLLLSIYIIESIAENIALRSQLIHSEKMTIVSELAASVAHEVRNPLTVVRGFVQLLFND
ETLQNKSSADYKKLVLSELDRAQGIITNYLDMAKQQLYEKEVFDLSALIKETSSLMVSYANYKSVTVEAETEPDLLIYGD
ATKLKQAVINLMKNSIEAVPHGKGMIHISAKRNGHTIMINITDNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSIIE
HHHGTISFNSSFQKGTTVTIKLPADLPH

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the kinB activity

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=31.1688311688312, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=31.858407079646, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=26.1261261261261, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790300, Length=235, Percent_Identity=27.2340425531915, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788393, Length=265, Percent_Identity=25.6603773584906, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=27.7533039647577, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693157, Length=256, Percent_Identity=26.953125, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=26.5486725663717, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790861, Length=232, Percent_Identity=23.7068965517241, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081816, Length=220, Percent_Identity=24.0909090909091, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789808, Length=214, Percent_Identity=24.2990654205607, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786783, Length=228, Percent_Identity=23.2456140350877, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=27.3504273504274, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=25.9574468085106, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI48994928, Length=233, Percent_Identity=23.175965665236, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KINB_BACSU (Q08430)

Other databases:

- EMBL:   U63302
- EMBL:   Z93933
- EMBL:   AL009126
- PIR:   S32935
- RefSeq:   NP_391023.2
- ProteinModelPortal:   Q08430
- SMR:   Q08430
- EnsemblBacteria:   EBBACT00000001557
- GeneID:   937167
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU31450
- NMPDR:   fig|224308.1.peg.3148
- GenoList:   BSU31450
- GeneTree:   EBGT00070000031970
- HOGENOM:   HBG341199
- ProtClustDB:   CLSK887792
- BioCyc:   BSUB:BSU31450-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 47813; Mature: 47813

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11bfa6d0)-; HASH(0x119f9514)-; HASH(0x1032370c)-; HASH(0x116c6580)-; HASH(0x1158a30c)-; HASH(0xd20d3d8)-;

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILKDYLLHICFILFPILLYQVFWLGKPAILVPKINSGLVTLFACGASVLCIIFPIHEM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
DYIQYGLQMIPVIICLFYISTASGLTVAASVLCFELLFYEPSAMFVFTLLPFLIIIPILF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QKKWPFMSKAKKLLLSLLISCVEIFLFFASSWILSALNILNFQKSGIFVYEAAVSGLFRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
SVLLLSIYIIESIAENIALRSQLIHSEKMTIVSELAASVAHEVRNPLTVVRGFVQLLFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
ETLQNKSSADYKKLVLSELDRAQGIITNYLDMAKQQLYEKEVFDLSALIKETSSLMVSYA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
NYKSVTVEAETEPDLLIYGDATKLKQAVINLMKNSIEAVPHGKGMIHISAKRNGHTIMIN
CCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
ITDNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSIIEHHHGTISFNSSFQKGTTVTI
EECCCCCCCHHHHHHHCCCCEEEECCCCCEEHEEEEEEHHHCCCEEEECCCCCCCCEEEE
KLPADLPH
EECCCCCH
>Mature Secondary Structure
MEILKDYLLHICFILFPILLYQVFWLGKPAILVPKINSGLVTLFACGASVLCIIFPIHEM
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
DYIQYGLQMIPVIICLFYISTASGLTVAASVLCFELLFYEPSAMFVFTLLPFLIIIPILF
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QKKWPFMSKAKKLLLSLLISCVEIFLFFASSWILSALNILNFQKSGIFVYEAAVSGLFRS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHH
SVLLLSIYIIESIAENIALRSQLIHSEKMTIVSELAASVAHEVRNPLTVVRGFVQLLFND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCC
ETLQNKSSADYKKLVLSELDRAQGIITNYLDMAKQQLYEKEVFDLSALIKETSSLMVSYA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
NYKSVTVEAETEPDLLIYGDATKLKQAVINLMKNSIEAVPHGKGMIHISAKRNGHTIMIN
CCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
ITDNGVGMTDHQMQKLGEPYYSLKTNGTGLGLTVTFSIIEHHHGTISFNSSFQKGTTVTI
EECCCCCCCHHHHHHHCCCCEEEECCCCCEEHEEEEEEHHHCCCEEEECCCCCCCCEEEE
KLPADLPH
EECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377