Click here to switch to the map view.

The map label for this gene is pckA

Identifier: 255767698

GI number: 255767698

Start: 3129530

End: 3131113

Strand: Direct

Name: pckA

Synonym: BSU30560

Alternate gene names: 255767698

Gene position: 3129530-3131113 (Clockwise)

Preceding gene: 16080104

Following gene: 255767699

Centisome position: 74.24

GC content: 47.22

Gene sequence:

>1584_bases
ATGAACTCAGTTGATTTGACCGCTGATTTACAAGCCTTATTAACATGTCCAAATGTGCGTCATAATTTATCAGCAGCACA
GCTAACAGAAAAAGTCCTCTCCCGAAACGAAGGCATTTTAACATCCACAGGTGCTGTTCGCGCGACAACAGGCGCTTACA
CAGGACGCTCACCTAAAGATAAATTCATCGTGGAGGAAGAAAGCACGAAAAATAAGATCGATTGGGGCCCGGTGAATCAG
CCGATTTCAGAAGAAGCGTTTGAGCGGCTGTACACGAAAGTTGTCAGCTATTTAAAGGAGCGAGATGAACTGTTTGTTTT
CGAAGGATTTGCCGGAGCAGACGAGAAATACAGGCTGCCGATCACTGTCGTAAATGAGTTCGCATGGCACAATTTATTTG
CGCGGCAGCTGTTTATCCGTCCGGAAGGAAATGATAAGAAAACAGTTGAGCAGCCGTTCACCATTCTTTCTGCTCCGCAT
TTCAAAGCGGATCCAAAAACAGACGGCACTCATTCCGAAACGTTTATTATTGTCTCTTTCGAAAAGCGGACAATTTTAAT
CGGCGGAACTGAGTATGCCGGTGAAATGAAGAAGTCCATTTTCTCCATTATGAATTTCCTGCTGCCTGAAAGAGATATTT
TATCTATGCACTGCTCCGCCAATGTCGGTGAAAAAGGCGATGTCGCCCTTTTCTTCGGACTGTCAGGAACAGGAAAGACC
ACCCTGTCGGCAGATGCTGACCGCAAGCTGATCGGTGACGATGAACATGGCTGGTCTGATACAGGCGTCTTTAATATTGA
AGGCGGATGCTACGCTAAGTGTATTCATTTAAGCGAGGAAAAGGAGCCGCAAATCTTTAACGCGATCCGCTTCGGGTCTG
TTCTCGAAAATGTCGTTGTGGATGAAGATACACGCGAAGCCAATTATGATGATTCCTTCTATACTGAAAACACGCGGGCA
GCTTACCCGATTCATATGATTAATAACATCGTGACTCCAAGCATGGCCGGCCATCCGTCAGCCATTGTATTTTTGACGGC
TGATGCCTTCGGAGTCCTGCCGCCGATCAGCAAACTAACGAAGGAGCAGGCGATGTACCATTTTTTGAGCGGTTACACGA
GTAAGCTTGCCGGAACCGAACGTGGTGTCACGTCTCCTGAAACGACGTTTTCTACATGCTTCGGCTCACCGTTCCTGCCG
CTTCCTGCTCACGTCTATGCTGAAATGCTCGGCAAAAAGATCGATGAACACGGCGCAGACGTTTTCTTAGTCAATACCGG
ATGGACCGGGGGCGGCTACGGCACAGGCGAACGAATGAAGCTTTCTTACACTAGAGCAATGGTCAAAGCAGCGATTGAAG
GCAAATTAGAGGATGCTGAAATGATAACTGACGATATTTTCGGCCTGCACATTCCGGCCCATGTTCCTGGCGTTCCTGAT
CATATCCTTCAGCCTGAAAACACGTGGACCAACAAGGAAGAATACAAAGAAAAAGCAGTCTACCTTGCAAATGAATTCAA
AGAGAACTTTAAAAAGTTCGCACATACCGATGCCATCGCCCAGGCAGGCGGCCCTCTCGTATAA

Upstream 100 bases:

>100_bases
GCACACACAAAAAACAATAAATAGTATAGACTATTTGAAAATATATGTTATACTAATTCACAATTAGCAAAACACAAAAA
ACGATAAAGGAAGGTTTCAT

Downstream 100 bases:

>100_bases
AAAACAAAAGCCAAGAGCAATTATGCTCTTGGCTTGTTTTAATTGACGGAATGCAAAGAAGTCAGCTTGTATGTAATGAT
GGTCCGGCCTTCTTCCCATT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MNSVDLTADLQALLTCPNVRHNLSAAQLTEKVLSRNEGILTSTGAVRATTGAYTGRSPKDKFIVEEESTKNKIDWGPVNQ
PISEEAFERLYTKVVSYLKERDELFVFEGFAGADEKYRLPITVVNEFAWHNLFARQLFIRPEGNDKKTVEQPFTILSAPH
FKADPKTDGTHSETFIIVSFEKRTILIGGTEYAGEMKKSIFSIMNFLLPERDILSMHCSANVGEKGDVALFFGLSGTGKT
TLSADADRKLIGDDEHGWSDTGVFNIEGGCYAKCIHLSEEKEPQIFNAIRFGSVLENVVVDEDTREANYDDSFYTENTRA
AYPIHMINNIVTPSMAGHPSAIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTSPETTFSTCFGSPFLP
LPAHVYAEMLGKKIDEHGADVFLVNTGWTGGGYGTGERMKLSYTRAMVKAAIEGKLEDAEMITDDIFGLHIPAHVPGVPD
HILQPENTWTNKEEYKEKAVYLANEFKENFKKFAHTDAIAQAGGPLV

Sequences:

>Translated_527_residues
MNSVDLTADLQALLTCPNVRHNLSAAQLTEKVLSRNEGILTSTGAVRATTGAYTGRSPKDKFIVEEESTKNKIDWGPVNQ
PISEEAFERLYTKVVSYLKERDELFVFEGFAGADEKYRLPITVVNEFAWHNLFARQLFIRPEGNDKKTVEQPFTILSAPH
FKADPKTDGTHSETFIIVSFEKRTILIGGTEYAGEMKKSIFSIMNFLLPERDILSMHCSANVGEKGDVALFFGLSGTGKT
TLSADADRKLIGDDEHGWSDTGVFNIEGGCYAKCIHLSEEKEPQIFNAIRFGSVLENVVVDEDTREANYDDSFYTENTRA
AYPIHMINNIVTPSMAGHPSAIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTSPETTFSTCFGSPFLP
LPAHVYAEMLGKKIDEHGADVFLVNTGWTGGGYGTGERMKLSYTRAMVKAAIEGKLEDAEMITDDIFGLHIPAHVPGVPD
HILQPENTWTNKEEYKEKAVYLANEFKENFKKFAHTDAIAQAGGPLV
>Mature_527_residues
MNSVDLTADLQALLTCPNVRHNLSAAQLTEKVLSRNEGILTSTGAVRATTGAYTGRSPKDKFIVEEESTKNKIDWGPVNQ
PISEEAFERLYTKVVSYLKERDELFVFEGFAGADEKYRLPITVVNEFAWHNLFARQLFIRPEGNDKKTVEQPFTILSAPH
FKADPKTDGTHSETFIIVSFEKRTILIGGTEYAGEMKKSIFSIMNFLLPERDILSMHCSANVGEKGDVALFFGLSGTGKT
TLSADADRKLIGDDEHGWSDTGVFNIEGGCYAKCIHLSEEKEPQIFNAIRFGSVLENVVVDEDTREANYDDSFYTENTRA
AYPIHMINNIVTPSMAGHPSAIVFLTADAFGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTSPETTFSTCFGSPFLP
LPAHVYAEMLGKKIDEHGADVFLVNTGWTGGGYGTGERMKLSYTRAMVKAAIEGKLEDAEMITDDIFGLHIPAHVPGVPD
HILQPENTWTNKEEYKEKAVYLANEFKENFKKFAHTDAIAQAGGPLV

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=513, Percent_Identity=49.9025341130604, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6322950, Length=501, Percent_Identity=51.6966067864271, Blast_Score=508, Evalue=1e-144,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_BACSU (P54418)

Other databases:

- EMBL:   AF008220
- EMBL:   AL009126
- EMBL:   U52812
- PIR:   F69673
- RefSeq:   NP_390934.2
- ProteinModelPortal:   P54418
- SMR:   P54418
- EnsemblBacteria:   EBBACT00000001279
- GeneID:   937235
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU30560
- NMPDR:   fig|224308.1.peg.3059
- GenoList:   BSU30560
- GeneTree:   EBGT00050000001183
- HOGENOM:   HBG293387
- PhylomeDB:   P54418
- ProtClustDB:   PRK09344
- BioCyc:   BSUB:BSU30560-MONOMER
- BRENDA:   4.1.1.49
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58272; Mature: 58272

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVDLTADLQALLTCPNVRHNLSAAQLTEKVLSRNEGILTSTGAVRATTGAYTGRSPKD
CCCCCCHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCC
KFIVEEESTKNKIDWGPVNQPISEEAFERLYTKVVSYLKERDELFVFEGFAGADEKYRLP
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECC
ITVVNEFAWHNLFARQLFIRPEGNDKKTVEQPFTILSAPHFKADPKTDGTHSETFIIVSF
HHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCHHEEECCCCCCCCCCCCCCCCEEEEEEE
EKRTILIGGTEYAGEMKKSIFSIMNFLLPERDILSMHCSANVGEKGDVALFFGLSGTGKT
CCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCEEEEEEECCCCCE
TLSADADRKLIGDDEHGWSDTGVFNIEGGCYAKCIHLSEEKEPQIFNAIRFGSVLENVVV
EECCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
DEDTREANYDDSFYTENTRAAYPIHMINNIVTPSMAGHPSAIVFLTADAFGVLPPISKLT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHH
KEQAMYHFLSGYTSKLAGTERGVTSPETTFSTCFGSPFLPLPAHVYAEMLGKKIDEHGAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
VFLVNTGWTGGGYGTGERMKLSYTRAMVKAAIEGKLEDAEMITDDIFGLHIPAHVPGVPD
EEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCCH
HILQPENTWTNKEEYKEKAVYLANEFKENFKKFAHTDAIAQAGGPLV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNSVDLTADLQALLTCPNVRHNLSAAQLTEKVLSRNEGILTSTGAVRATTGAYTGRSPKD
CCCCCCHHHHHHHHCCCCCHHCCHHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCC
KFIVEEESTKNKIDWGPVNQPISEEAFERLYTKVVSYLKERDELFVFEGFAGADEKYRLP
CEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECC
ITVVNEFAWHNLFARQLFIRPEGNDKKTVEQPFTILSAPHFKADPKTDGTHSETFIIVSF
HHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCHHEEECCCCCCCCCCCCCCCCEEEEEEE
EKRTILIGGTEYAGEMKKSIFSIMNFLLPERDILSMHCSANVGEKGDVALFFGLSGTGKT
CCCEEEEECCHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCEEEEEEECCCCCE
TLSADADRKLIGDDEHGWSDTGVFNIEGGCYAKCIHLSEEKEPQIFNAIRFGSVLENVVV
EECCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
DEDTREANYDDSFYTENTRAAYPIHMINNIVTPSMAGHPSAIVFLTADAFGVLPPISKLT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHH
KEQAMYHFLSGYTSKLAGTERGVTSPETTFSTCFGSPFLPLPAHVYAEMLGKKIDEHGAD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
VFLVNTGWTGGGYGTGERMKLSYTRAMVKAAIEGKLEDAEMITDDIFGLHIPAHVPGVPD
EEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCCCCCCCH
HILQPENTWTNKEEYKEKAVYLANEFKENFKKFAHTDAIAQAGGPLV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891