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The map label for this gene is serA

Identifier: 255767495

GI number: 255767495

Start: 2411086

End: 2412663

Strand: Direct

Name: serA

Synonym: BSU23070

Alternate gene names: 255767495

Gene position: 2411086-2412663 (Clockwise)

Preceding gene: 16079361

Following gene: 16079380

Centisome position: 57.19

GC content: 42.78

Gene sequence:

>1578_bases
ATGTTTCGAGTATTGGTCTCAGACAAAATGAGCAACGACGGTTTACAGCCACTTATTGAATCAGACTTTATTGAAATCGT
TCAAAAAAACGTAGCAGATGCAGAAGATGAATTACATACTTTTGATGCTCTTTTGGTGCGAAGCGCGACAAAAGTAACAG
AAGACCTTTTCAACAAAATGACTTCTTTAAAAATTGTCGGAAGAGCCGGTGTCGGTGTCGATAATATCGATATTGATGAG
GCTACGAAACACGGGGTAATCGTGATCAATGCTCCGAACGGCAACACCATTTCGACAGCTGAGCATACATTTGCAATGAT
CTCTTCTTTAATGAGACACATTCCGCAGGCTAATATCTCAGTGAAATCCAGAGAGTGGAACCGCACAGCTTATGTCGGTT
CAGAGCTTTATGGAAAAACGCTTGGTATTGTAGGATTAGGCCGAATCGGAAGTGAAATTGCACAGCGTGCGAGAGCGTTC
GGTATGACTGTGCACGTTTTTGATCCTTTCTTAACGGAAGAAAGAGCGAAAAAAATCGGCGTAAACAGCCGTACATTTGA
AGAAGTGCTTGAAAGTGCGGATATCATTACCGTTCACACGCCTTTAACAAAAGAAACAAAAGGCTTATTGAATAAAGAAA
CGATTGCAAAAACGAAAAAAGGCGTTCGCTTAATTAACTGTGCGCGAGGCGGAATTATCGATGAAGCAGCACTTCTCGAA
GCTTTGGAAAACGGGCATGTTGCGGGAGCAGCGCTTGACGTTTTCGAAGTCGAACCGCCAGTTGACAACAAACTTGTTGA
TCATCCATTAGTCATTGCCACTCCTCACTTGGGAGCATCAACGAAAGAAGCACAGCTCAATGTCGCCGCCCAAGTGTCAG
AAGAAGTTCTGCAGTTCGCAAAAGGCCTGCCTGTCATGTCAGCTATCAACCTGCCTGCCATGACAAAAGATGAATTTGCT
AAAATTAAGCCTTACCATCAAATTGCAGGAAAAATTGGCAGCCTTGTATCACAGTGCATGAAAGAACCAGTACAGGATGT
TGCCATTCAATATGAAGGCACAATTGCTAAACTTGAAACATCGTTCATTACAAAAGCCCTTCTGTCAGGCTTTTTAAAAC
CGCGCGTAGACTCTACTGTTAATGAGGTCAATGCCGGGGGTGTCGCAAAAGAACGCGGCATTAGCTTCAGTGAAAAAATT
TCCTCTTCTGAATCTGGATACGATAACTGCATCAGCGTAAAAGTGACAGGAGACCGCAGCACCTTTACTGTAACGGCTAC
GTATATTCCTCATTTCGGAGAACGAATTGTCGAGATCAATGGATTTAATATTGATTTTTATCCGACTGGTCACTTGGTGT
ATATCCAGCATCAAGATACAACAGGTGTCATCGGCCGAGTAGGACGTATTCTCGGAGATAATGATATTAACATTGCAACT
ATGCAGGTTGGCCGTAAGGAAAAAGGCGGAGAAGCCATCATGATGCTTTCCTTTGACAGACATCTGGAAGATAAAATTGT
GAAAGAACTAACAAATGTCCCTGATATTGTGTCTGTGAAGCTCATTGATCTGCCATAA

Upstream 100 bases:

>100_bases
TTACTTTTATCCTTTACTGCGTCAATACACGTTGACACTCTTTTGAGAATATGTTAAATTATCAGATATTTAGTTTGTCA
ATTTAGGAGGAAATCTAACG

Downstream 100 bases:

>100_bases
TTAAAAAAACTCAAGCTATATAGCTTGAGTTTTTTTATTGTTCTATCCCCGTGTGTTTTTATGCAAAATATCTAAGACAC
TCCTTAGCTCAGAAACAGGA

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: PGDH

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MFRVLVSDKMSNDGLQPLIESDFIEIVQKNVADAEDELHTFDALLVRSATKVTEDLFNKMTSLKIVGRAGVGVDNIDIDE
ATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQANISVKSREWNRTAYVGSELYGKTLGIVGLGRIGSEIAQRARAF
GMTVHVFDPFLTEERAKKIGVNSRTFEEVLESADIITVHTPLTKETKGLLNKETIAKTKKGVRLINCARGGIIDEAALLE
ALENGHVAGAALDVFEVEPPVDNKLVDHPLVIATPHLGASTKEAQLNVAAQVSEEVLQFAKGLPVMSAINLPAMTKDEFA
KIKPYHQIAGKIGSLVSQCMKEPVQDVAIQYEGTIAKLETSFITKALLSGFLKPRVDSTVNEVNAGGVAKERGISFSEKI
SSSESGYDNCISVKVTGDRSTFTVTATYIPHFGERIVEINGFNIDFYPTGHLVYIQHQDTTGVIGRVGRILGDNDINIAT
MQVGRKEKGGEAIMMLSFDRHLEDKIVKELTNVPDIVSVKLIDLP

Sequences:

>Translated_525_residues
MFRVLVSDKMSNDGLQPLIESDFIEIVQKNVADAEDELHTFDALLVRSATKVTEDLFNKMTSLKIVGRAGVGVDNIDIDE
ATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQANISVKSREWNRTAYVGSELYGKTLGIVGLGRIGSEIAQRARAF
GMTVHVFDPFLTEERAKKIGVNSRTFEEVLESADIITVHTPLTKETKGLLNKETIAKTKKGVRLINCARGGIIDEAALLE
ALENGHVAGAALDVFEVEPPVDNKLVDHPLVIATPHLGASTKEAQLNVAAQVSEEVLQFAKGLPVMSAINLPAMTKDEFA
KIKPYHQIAGKIGSLVSQCMKEPVQDVAIQYEGTIAKLETSFITKALLSGFLKPRVDSTVNEVNAGGVAKERGISFSEKI
SSSESGYDNCISVKVTGDRSTFTVTATYIPHFGERIVEINGFNIDFYPTGHLVYIQHQDTTGVIGRVGRILGDNDINIAT
MQVGRKEKGGEAIMMLSFDRHLEDKIVKELTNVPDIVSVKLIDLP
>Mature_525_residues
MFRVLVSDKMSNDGLQPLIESDFIEIVQKNVADAEDELHTFDALLVRSATKVTEDLFNKMTSLKIVGRAGVGVDNIDIDE
ATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQANISVKSREWNRTAYVGSELYGKTLGIVGLGRIGSEIAQRARAF
GMTVHVFDPFLTEERAKKIGVNSRTFEEVLESADIITVHTPLTKETKGLLNKETIAKTKKGVRLINCARGGIIDEAALLE
ALENGHVAGAALDVFEVEPPVDNKLVDHPLVIATPHLGASTKEAQLNVAAQVSEEVLQFAKGLPVMSAINLPAMTKDEFA
KIKPYHQIAGKIGSLVSQCMKEPVQDVAIQYEGTIAKLETSFITKALLSGFLKPRVDSTVNEVNAGGVAKERGISFSEKI
SSSESGYDNCISVKVTGDRSTFTVTATYIPHFGERIVEINGFNIDFYPTGHLVYIQHQDTTGVIGRVGRILGDNDINIAT
MQVGRKEKGGEAIMMLSFDRHLEDKIVKELTNVPDIVSVKLIDLP

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain

Homologues:

Organism=Homo sapiens, GI23308577, Length=416, Percent_Identity=42.7884615384615, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=36.2962962962963, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=36.2962962962963, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI145580578, Length=293, Percent_Identity=34.1296928327645, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI4557499, Length=293, Percent_Identity=34.1296928327645, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI145580575, Length=264, Percent_Identity=35.6060606060606, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI6912396, Length=257, Percent_Identity=30.7392996108949, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1789279, Length=320, Percent_Identity=32.1875, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082289, Length=266, Percent_Identity=34.5864661654135, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787645, Length=207, Percent_Identity=32.3671497584541, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788660, Length=268, Percent_Identity=33.5820895522388, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI87081824, Length=251, Percent_Identity=29.4820717131474, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17532191, Length=304, Percent_Identity=49.0131578947368, Blast_Score=299, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI25147481, Length=269, Percent_Identity=33.457249070632, Blast_Score=155, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=285, Percent_Identity=32.6315789473684, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322116, Length=300, Percent_Identity=31.6666666666667, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6324055, Length=292, Percent_Identity=32.8767123287671, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324964, Length=256, Percent_Identity=29.296875, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325144, Length=149, Percent_Identity=32.2147651006711, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321253, Length=260, Percent_Identity=25.7692307692308, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=190, Percent_Identity=28.9473684210526, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921140, Length=304, Percent_Identity=48.3552631578947, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24646446, Length=244, Percent_Identity=36.0655737704918, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24646448, Length=244, Percent_Identity=36.0655737704918, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24646452, Length=244, Percent_Identity=36.0655737704918, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24646450, Length=244, Percent_Identity=36.0655737704918, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI62472511, Length=244, Percent_Identity=36.0655737704918, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28574286, Length=252, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28571528, Length=276, Percent_Identity=32.9710144927536, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24585516, Length=315, Percent_Identity=24.1269841269841, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28574284, Length=318, Percent_Identity=24.8427672955975, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24585514, Length=318, Percent_Identity=24.8427672955975, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28574282, Length=318, Percent_Identity=24.8427672955975, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45552429, Length=318, Percent_Identity=24.8427672955975, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45551003, Length=318, Percent_Identity=24.8427672955975, Blast_Score=102, Evalue=8e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SERA_BACSU (P35136)

Other databases:

- EMBL:   L47648
- EMBL:   AL009126
- EMBL:   L09228
- PIR:   C69705
- RefSeq:   NP_390188.2
- ProteinModelPortal:   P35136
- SMR:   P35136
- EnsemblBacteria:   EBBACT00000002415
- GeneID:   938964
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU23070
- NMPDR:   fig|224308.1.peg.2311
- GenoList:   BSU23070
- GeneTree:   EBGT00050000001763
- HOGENOM:   HBG731446
- PhylomeDB:   P35136
- ProtClustDB:   PRK13581
- BioCyc:   BSUB:BSU23070-MONOMER
- BRENDA:   1.1.1.95
- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF20
- TIGRFAMs:   TIGR01327

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT

EC number: =1.1.1.95

Molecular weight: Translated: 57130; Mature: 57130

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 229-229 ACT_SITE 258-258 ACT_SITE 276-276 BINDING 168-168 BINDING 200-200 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRVLVSDKMSNDGLQPLIESDFIEIVQKNVADAEDELHTFDALLVRSATKVTEDLFNKM
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLKIVGRAGVGVDNIDIDEATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQANIS
HHEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCE
VKSREWNRTAYVGSELYGKTLGIVGLGRIGSEIAQRARAFGMTVHVFDPFLTEERAKKIG
EECCCCCCEEEECHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCC
VNSRTFEEVLESADIITVHTPLTKETKGLLNKETIAKTKKGVRLINCARGGIIDEAALLE
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
ALENGHVAGAALDVFEVEPPVDNKLVDHPLVIATPHLGASTKEAQLNVAAQVSEEVLQFA
HHHCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLPVMSAINLPAMTKDEFAKIKPYHQIAGKIGSLVSQCMKEPVQDVAIQYEGTIAKLET
CCCCCCHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHEEEECCCHHHHHH
SFITKALLSGFLKPRVDSTVNEVNAGGVAKERGISFSEKISSSESGYDNCISVKVTGDRS
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCCEEEEEEECCCC
TFTVTATYIPHFGERIVEINGFNIDFYPTGHLVYIQHQDTTGVIGRVGRILGDNDINIAT
EEEEEEEECCCCCCEEEEEECEEEEEEECCEEEEEEECCCCHHHHHHHHHHCCCCCEEEE
MQVGRKEKGGEAIMMLSFDRHLEDKIVKELTNVPDIVSVKLIDLP
EECCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECC
>Mature Secondary Structure
MFRVLVSDKMSNDGLQPLIESDFIEIVQKNVADAEDELHTFDALLVRSATKVTEDLFNKM
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSLKIVGRAGVGVDNIDIDEATKHGVIVINAPNGNTISTAEHTFAMISSLMRHIPQANIS
HHEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHCCCCCCE
VKSREWNRTAYVGSELYGKTLGIVGLGRIGSEIAQRARAFGMTVHVFDPFLTEERAKKIG
EECCCCCCEEEECHHHHCCCCCEEEEHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHCC
VNSRTFEEVLESADIITVHTPLTKETKGLLNKETIAKTKKGVRLINCARGGIIDEAALLE
CCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
ALENGHVAGAALDVFEVEPPVDNKLVDHPLVIATPHLGASTKEAQLNVAAQVSEEVLQFA
HHHCCCEEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLPVMSAINLPAMTKDEFAKIKPYHQIAGKIGSLVSQCMKEPVQDVAIQYEGTIAKLET
CCCCCCHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHEEEECCCHHHHHH
SFITKALLSGFLKPRVDSTVNEVNAGGVAKERGISFSEKISSSESGYDNCISVKVTGDRS
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCHHHHHCCCCCCCCCEEEEEEECCCC
TFTVTATYIPHFGERIVEINGFNIDFYPTGHLVYIQHQDTTGVIGRVGRILGDNDINIAT
EEEEEEEECCCCCCEEEEEECEEEEEEECCEEEEEEECCCCHHHHHHHHHHCCCCCEEEE
MQVGRKEKGGEAIMMLSFDRHLEDKIVKELTNVPDIVSVKLIDLP
EECCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 7934829