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The map label for this gene is ycjG [C]

Identifier: 239618114

GI number: 239618114

Start: 1876891

End: 1877922

Strand: Reverse

Name: ycjG [C]

Synonym: Kole_1746

Alternate gene names: 239618114

Gene position: 1877922-1876891 (Counterclockwise)

Preceding gene: 239618115

Following gene: 239618113

Centisome position: 81.57

GC content: 44.28

Gene sequence:

>1032_bases
ATGGGAAAGATAGTCGATGTGAAGTTTTTGGTAAAGGAATACGATTATGAAAAACCCTTTCATATCGCCAACAACATCTC
AACCAGCAGCACAAATGTAGAAGTGCAGATAGTACTTGACAATGGTCTAGTTGGTTATGGAGAAGCTGCCCCATCTTACA
GGGTGAACGGAGAACGAGTTGAAACCTTCCCGTCTCTGGAGCCTTTTGTTAAAGAACAACTTATTGGAATGGATGTAAGA
AATTACAAGCGCATCTTTGACATCATGGATGGCTTGCGCTGCGCTTCCAGTTTAAAAGCTGCTGTGCAATTCGCTGCTCT
GGATGCCATTAGCGTTGAAAGCGGGTTGCCTGTACATCAACTGTTAGGCGGAGCAAAAGACGAAATAGAAACAGATAAAA
CCGTCAGCATCGATACCATAGAAAACATGGTCAAAGACGCGAAGGAAATATACGATTCAGGATTCCGCGTGATAAAGATA
AAGGTCGGGGAAAATCTGAAGAAGGATATTGAGACGCTGGAAGCTATCGCTGAAGTGACAAAGGAAGCAAAATACATTGT
AGACGCGAACACGGGTTATACCCCCAAGGAAGCCATTGCGTTTGCAAAGGCCCTCTATTCCAAAGGCATAGATATAGACG
TATTCGAACAACCCGTACCGGCTGAAAGTATAGAAGGGCTAAAATTGGTCAGGTTCAATTCACCTTTCCCAGTCGCGGCC
GACGAAAGCGCAAGAACAAGATACGACGTTTTGAGACTCATACGGGAAGAAGCCGTTGATTTTGTGAATATAAAGCTGAT
GAAGTCAGGAATCTCCGACGCCCTTGCCATCGTGGAAATGGCGAATACCGCGAAGATTCAGTTGATGATAGGTTGTATGG
GTGAATCCAGTGTTGGAATAAATCAGAGCGTTCAATTTGCGCTGGGAACAGGAGCCTTCGTATTCCATGACCTCGATTCT
CACCTGATGCTTAAAGAAGAGCAATTTAGAGGAAAGTTTAAACAGGATGGGCCGAAGATGATTGTTCGTTAA

Upstream 100 bases:

>100_bases
GAACTTGAAAATATCTACAACGATGCTTTCACTTATGAAATGAAGGTACTTTCTTTCGAAGAGATTTGAAAAACAAAATA
TTTCTCGGGGAGGAAAGAGT

Downstream 100 bases:

>100_bases
ATAAAAAAGGGTGGTTTATCCGCCATTTTTTATGTTGGAAATTTTACGTCTTTTATGAAGTTTGCTGGTATAATGAAAAG
GTACCTGTGCGGTCAGGTAT

Product: Mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MGKIVDVKFLVKEYDYEKPFHIANNISTSSTNVEVQIVLDNGLVGYGEAAPSYRVNGERVETFPSLEPFVKEQLIGMDVR
NYKRIFDIMDGLRCASSLKAAVQFAALDAISVESGLPVHQLLGGAKDEIETDKTVSIDTIENMVKDAKEIYDSGFRVIKI
KVGENLKKDIETLEAIAEVTKEAKYIVDANTGYTPKEAIAFAKALYSKGIDIDVFEQPVPAESIEGLKLVRFNSPFPVAA
DESARTRYDVLRLIREEAVDFVNIKLMKSGISDALAIVEMANTAKIQLMIGCMGESSVGINQSVQFALGTGAFVFHDLDS
HLMLKEEQFRGKFKQDGPKMIVR

Sequences:

>Translated_343_residues
MGKIVDVKFLVKEYDYEKPFHIANNISTSSTNVEVQIVLDNGLVGYGEAAPSYRVNGERVETFPSLEPFVKEQLIGMDVR
NYKRIFDIMDGLRCASSLKAAVQFAALDAISVESGLPVHQLLGGAKDEIETDKTVSIDTIENMVKDAKEIYDSGFRVIKI
KVGENLKKDIETLEAIAEVTKEAKYIVDANTGYTPKEAIAFAKALYSKGIDIDVFEQPVPAESIEGLKLVRFNSPFPVAA
DESARTRYDVLRLIREEAVDFVNIKLMKSGISDALAIVEMANTAKIQLMIGCMGESSVGINQSVQFALGTGAFVFHDLDS
HLMLKEEQFRGKFKQDGPKMIVR
>Mature_342_residues
GKIVDVKFLVKEYDYEKPFHIANNISTSSTNVEVQIVLDNGLVGYGEAAPSYRVNGERVETFPSLEPFVKEQLIGMDVRN
YKRIFDIMDGLRCASSLKAAVQFAALDAISVESGLPVHQLLGGAKDEIETDKTVSIDTIENMVKDAKEIYDSGFRVIKIK
VGENLKKDIETLEAIAEVTKEAKYIVDANTGYTPKEAIAFAKALYSKGIDIDVFEQPVPAESIEGLKLVRFNSPFPVAAD
ESARTRYDVLRLIREEAVDFVNIKLMKSGISDALAIVEMANTAKIQLMIGCMGESSVGINQSVQFALGTGAFVFHDLDSH
LMLKEEQFRGKFKQDGPKMIVR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of a broad range of other

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=320, Percent_Identity=27.5, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 37933; Mature: 37802

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKIVDVKFLVKEYDYEKPFHIANNISTSSTNVEVQIVLDNGLVGYGEAAPSYRVNGERV
CCCEEEHHHHHHHCCCCCCCCHHCCCCCCCCCEEEEEEEECCEEECCCCCCCCCCCCCCC
ETFPSLEPFVKEQLIGMDVRNYKRIFDIMDGLRCASSLKAAVQFAALDAISVESGLPVHQ
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
LLGGAKDEIETDKTVSIDTIENMVKDAKEIYDSGFRVIKIKVGENLKKDIETLEAIAEVT
HHCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHH
KEAKYIVDANTGYTPKEAIAFAKALYSKGIDIDVFEQPVPAESIEGLKLVRFNSPFPVAA
HHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHCCCEEEEEECCCCCCCC
DESARTRYDVLRLIREEAVDFVNIKLMKSGISDALAIVEMANTAKIQLMIGCMGESSVGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEEEECCCCCCCC
NQSVQFALGTGAFVFHDLDSHLMLKEEQFRGKFKQDGPKMIVR
CCCEEEEECCCEEEEEECCCHHEEECHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKIVDVKFLVKEYDYEKPFHIANNISTSSTNVEVQIVLDNGLVGYGEAAPSYRVNGERV
CCEEEHHHHHHHCCCCCCCCHHCCCCCCCCCEEEEEEEECCEEECCCCCCCCCCCCCCC
ETFPSLEPFVKEQLIGMDVRNYKRIFDIMDGLRCASSLKAAVQFAALDAISVESGLPVHQ
CCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHH
LLGGAKDEIETDKTVSIDTIENMVKDAKEIYDSGFRVIKIKVGENLKKDIETLEAIAEVT
HHCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHH
KEAKYIVDANTGYTPKEAIAFAKALYSKGIDIDVFEQPVPAESIEGLKLVRFNSPFPVAA
HHCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHCCCEEEEEECCCCCCCC
DESARTRYDVLRLIREEAVDFVNIKLMKSGISDALAIVEMANTAKIQLMIGCMGESSVGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEEEECCCCCCCC
NQSVQFALGTGAFVFHDLDSHLMLKEEQFRGKFKQDGPKMIVR
CCCEEEEECCCEEEEEECCCHHEEECHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]