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The map label for this gene is ppdK [H]

Identifier: 239618111

GI number: 239618111

Start: 1871908

End: 1874556

Strand: Reverse

Name: ppdK [H]

Synonym: Kole_1743

Alternate gene names: 239618111

Gene position: 1874556-1871908 (Counterclockwise)

Preceding gene: 239618113

Following gene: 239618110

Centisome position: 81.43

GC content: 47.23

Gene sequence:

>2649_bases
GTGTCTAAGAAGTACGTATACTTTTTCGGAAAAGGCAGGGCTGAGGGAGACGCTTCTATGAAGCAGCTCCTCGGTGGTAA
AGGCGCGAACCTCGCTGAAATGGTGCGCCTTGGAGTTCCCGTACCACCTGGTTTTACAATCTCCACTGAAGTCTGTAAAT
ATTACTACGACCATGGAAAAACGTATCCTGAAGAGCTAAAGGATCAGGTACTCAAAGCTATGAAAGAACTCGAGGAAGCT
ACTGGAAAGGTCTTTGGTTCTCCTGAAAATCCTCTCCTTGTTTCCGTTAGATCTGGTGCCGCAGTTTCTATGCCTGGTAT
GATGGACACAATCCTCAACCTTGGCTTGAACGACGAAACCGTTGAAGGTATGATAAAGTCCACAAATAATCCAAGATTCT
GCTACGATGCCTACCGCAGGTTTATGCAGATGTTTGGCGATGTTGTTCTGAACATCAAGCATGAGTACTTCGAAGAGGAA
CTCAATAAAGTCAAGACCGAAAAAGGTGTCAAGCTGGATACCGATCTTGATGCCGATGACCTCAAAAAGGTTGTAGAAAG
ATACAAAGAGGTTTATAAGAAAGCAGGACACAAATTCCCGCAGGATCCATGGGATCAGCTCTGGAAGGCTATCGATGCCG
TCTTTGGAAGCTGGATGAATGAAAGGGCCATTAAGTACAGAGAACTTAACGGCATAAAGGAAGGTTCTCTCCTAGGTACG
GCTGTCAACGTTGTTGCGATGGTTTTCGGTAACATGGGCGAAGACAGTGGTACCGGCGTTGCCTTTACAAGAGATCCAAA
CACCGGTGAAAAGAAGTACTACGGAGAATATCTCCCAAACGCCCAGGGTGAAGACGTTGTTGCCGGTATAAGAACCCCAT
ATCCTCTGACAAAACTCGAAGAAATGATGCCGGACGTTTATAAACAGCTCCTCGATATATTCGAGAAACTTGAAAAGCAC
TACAGGGATATGCAGGATATAGAATTTACCATTGAACGTGGAAAACTCTATCTCCTCCAGACAAGAACAGGAAAGAGAAC
CCCATTCGCCGCTGTGAGGATCGCTGTCGATATGGCAGAAGAAGGGCTCATAACCAAAGAAGAAGCCATTATGAGGGTCT
CCCCAGATCACATCGAAACCCTGCTGCACCCCTACTTTGAACCCGAAACCAAGAAAAACGCTTCCCTTCTCGCTAAGGGT
ATAGCCGCTTCTCCGGGAGCAGCAACGGGAATCATTGTCTTTGATCCTGCAGAAGCCGAAAGAATGGTCAAAGAGGAAAA
CAAACAGGTAATCCTTGTCAGACCCGAAACGTCTCCGGAAGATATAGCCGGAATGGCAGCAGCTGAAGGTATCCTCACAT
CCACCGGCGGTAAAACCTCTCACGCCGCTGTTGTTGCCAGAGGTATGGGCAAACCCTGTATTGTTGGCTGTGAAGCTATC
GAGGTGGACGAAAAGGCTGGCGTTATAAGAATAGATGGCAAAGAGATTAAGGAGTTCTCCTGGCTCAGTATAGACGGAAC
GACGGGTGAGGTATTCGACGGCAAGCTTGCCACCGTGAAACCTCAGGGACTTGAAGGACCTCTCGCAAAGATATTGCAAT
GGGCCGACGAGATCAGAAAACTCGGTGTCAGGACCAACGCTGATACCCCGAGAGACGCGACGGTTGCCAGAAACTTCGGC
GCTGAAGGTATCGGTCTGTGTAGAACAGAGCATATGTTCTTCGAAGAAGAGAGAATCCCCGTCGTTAGACAAATGATTGT
AGCCAAGACAAAGGAACAGCGCGAAATAGCTCTTGAAAAACTTCTCCCAATGCAGCAGGAAGACTTTGTTGGACTATTCA
AGGCAATGGAAGGTTATCCTGTTACAATCAGACTTCTCGACCCACCTCTTCACGAGTTCCTACCGAAGGAAGACGAGGAC
ATCAAAGCCCTTGCGGAAGAAATGGGACTCTCCTACGAAGAGCTCAAGGCAACTGTTGAAGAACTCCACGAGTTCAACCC
GATGATGGGACACAGAGGCTGCCGTTTAAGTATTACATATCCAGAGATCGCTGTCATGCAGACCAAAGCAATTATCGGTG
CAGCCATTCAGGTGCTCAAAGAAGAAGGAAAAACGGTCATTCCTGAAATAATGATTCCTCTTGTTGGTCACATTAACGAA
CTGAAGTATCTCAAAAAGATAATTACCGAAACCGCCGATAAGATGATAGAAGAAGCTGGTGTGGATCTGCAGTACAAGGT
GGGAACAATGATCGAAATTCCAAGAGCTGCTCTCACTGCAGACGAAATAGCAACCGAAGCTGAATTCTTCAGTTTCGGAA
CAAATGACCTCACACAGATGACCTTCGGATTCAGCCGTGACGACTACGGAAAGTTCGTAAAAGAATACATAGAAAAGGGC
ATTCTCGAGGTTGATCCATTCCAGAAACTCGACAGAACTGGTGTCGGCCAGCTCGTTAAGATGGGTACCGAAAAGGGCAG
GTCAACAAGACCAAACCTGAAAGTTGGTATCTGTGGCGAGCACGGAGGCGAACCCTCTTCAATTGAATTCTGTCATATAA
CCGGTCTCGACTACGTGAGCTGCTCTCCTTACAGAGTACCCGTTGCAAGACTTGCCGCTGCTCAGGCAGCAATAAAGCAT
GGTAAATAA

Upstream 100 bases:

>100_bases
TAATTGGAGGGTCTTTAGTACAAATTCGAGTTACAGCATATTGCGCCCTGTCATGTTATAATTCAACACGAATATGTTCG
TATCGAAAGGAGGAAAAACC

Downstream 100 bases:

>100_bases
TGAACACTATTGCCGCGACTCCAATGCGGGTCGCGGCTTTATTTTTTTGGAGGGTTGATGATGCTGGAAGCGATCCTTAA
ATTCGTTAGCTCAACGGGGT

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 882; Mature: 881

Protein sequence:

>882_residues
MSKKYVYFFGKGRAEGDASMKQLLGGKGANLAEMVRLGVPVPPGFTISTEVCKYYYDHGKTYPEELKDQVLKAMKELEEA
TGKVFGSPENPLLVSVRSGAAVSMPGMMDTILNLGLNDETVEGMIKSTNNPRFCYDAYRRFMQMFGDVVLNIKHEYFEEE
LNKVKTEKGVKLDTDLDADDLKKVVERYKEVYKKAGHKFPQDPWDQLWKAIDAVFGSWMNERAIKYRELNGIKEGSLLGT
AVNVVAMVFGNMGEDSGTGVAFTRDPNTGEKKYYGEYLPNAQGEDVVAGIRTPYPLTKLEEMMPDVYKQLLDIFEKLEKH
YRDMQDIEFTIERGKLYLLQTRTGKRTPFAAVRIAVDMAEEGLITKEEAIMRVSPDHIETLLHPYFEPETKKNASLLAKG
IAASPGAATGIIVFDPAEAERMVKEENKQVILVRPETSPEDIAGMAAAEGILTSTGGKTSHAAVVARGMGKPCIVGCEAI
EVDEKAGVIRIDGKEIKEFSWLSIDGTTGEVFDGKLATVKPQGLEGPLAKILQWADEIRKLGVRTNADTPRDATVARNFG
AEGIGLCRTEHMFFEEERIPVVRQMIVAKTKEQREIALEKLLPMQQEDFVGLFKAMEGYPVTIRLLDPPLHEFLPKEDED
IKALAEEMGLSYEELKATVEELHEFNPMMGHRGCRLSITYPEIAVMQTKAIIGAAIQVLKEEGKTVIPEIMIPLVGHINE
LKYLKKIITETADKMIEEAGVDLQYKVGTMIEIPRAALTADEIATEAEFFSFGTNDLTQMTFGFSRDDYGKFVKEYIEKG
ILEVDPFQKLDRTGVGQLVKMGTEKGRSTRPNLKVGICGEHGGEPSSIEFCHITGLDYVSCSPYRVPVARLAAAQAAIKH
GK

Sequences:

>Translated_882_residues
MSKKYVYFFGKGRAEGDASMKQLLGGKGANLAEMVRLGVPVPPGFTISTEVCKYYYDHGKTYPEELKDQVLKAMKELEEA
TGKVFGSPENPLLVSVRSGAAVSMPGMMDTILNLGLNDETVEGMIKSTNNPRFCYDAYRRFMQMFGDVVLNIKHEYFEEE
LNKVKTEKGVKLDTDLDADDLKKVVERYKEVYKKAGHKFPQDPWDQLWKAIDAVFGSWMNERAIKYRELNGIKEGSLLGT
AVNVVAMVFGNMGEDSGTGVAFTRDPNTGEKKYYGEYLPNAQGEDVVAGIRTPYPLTKLEEMMPDVYKQLLDIFEKLEKH
YRDMQDIEFTIERGKLYLLQTRTGKRTPFAAVRIAVDMAEEGLITKEEAIMRVSPDHIETLLHPYFEPETKKNASLLAKG
IAASPGAATGIIVFDPAEAERMVKEENKQVILVRPETSPEDIAGMAAAEGILTSTGGKTSHAAVVARGMGKPCIVGCEAI
EVDEKAGVIRIDGKEIKEFSWLSIDGTTGEVFDGKLATVKPQGLEGPLAKILQWADEIRKLGVRTNADTPRDATVARNFG
AEGIGLCRTEHMFFEEERIPVVRQMIVAKTKEQREIALEKLLPMQQEDFVGLFKAMEGYPVTIRLLDPPLHEFLPKEDED
IKALAEEMGLSYEELKATVEELHEFNPMMGHRGCRLSITYPEIAVMQTKAIIGAAIQVLKEEGKTVIPEIMIPLVGHINE
LKYLKKIITETADKMIEEAGVDLQYKVGTMIEIPRAALTADEIATEAEFFSFGTNDLTQMTFGFSRDDYGKFVKEYIEKG
ILEVDPFQKLDRTGVGQLVKMGTEKGRSTRPNLKVGICGEHGGEPSSIEFCHITGLDYVSCSPYRVPVARLAAAQAAIKH
GK
>Mature_881_residues
SKKYVYFFGKGRAEGDASMKQLLGGKGANLAEMVRLGVPVPPGFTISTEVCKYYYDHGKTYPEELKDQVLKAMKELEEAT
GKVFGSPENPLLVSVRSGAAVSMPGMMDTILNLGLNDETVEGMIKSTNNPRFCYDAYRRFMQMFGDVVLNIKHEYFEEEL
NKVKTEKGVKLDTDLDADDLKKVVERYKEVYKKAGHKFPQDPWDQLWKAIDAVFGSWMNERAIKYRELNGIKEGSLLGTA
VNVVAMVFGNMGEDSGTGVAFTRDPNTGEKKYYGEYLPNAQGEDVVAGIRTPYPLTKLEEMMPDVYKQLLDIFEKLEKHY
RDMQDIEFTIERGKLYLLQTRTGKRTPFAAVRIAVDMAEEGLITKEEAIMRVSPDHIETLLHPYFEPETKKNASLLAKGI
AASPGAATGIIVFDPAEAERMVKEENKQVILVRPETSPEDIAGMAAAEGILTSTGGKTSHAAVVARGMGKPCIVGCEAIE
VDEKAGVIRIDGKEIKEFSWLSIDGTTGEVFDGKLATVKPQGLEGPLAKILQWADEIRKLGVRTNADTPRDATVARNFGA
EGIGLCRTEHMFFEEERIPVVRQMIVAKTKEQREIALEKLLPMQQEDFVGLFKAMEGYPVTIRLLDPPLHEFLPKEDEDI
KALAEEMGLSYEELKATVEELHEFNPMMGHRGCRLSITYPEIAVMQTKAIIGAAIQVLKEEGKTVIPEIMIPLVGHINEL
KYLKKIITETADKMIEEAGVDLQYKVGTMIEIPRAALTADEIATEAEFFSFGTNDLTQMTFGFSRDDYGKFVKEYIEKGI
LEVDPFQKLDRTGVGQLVKMGTEKGRSTRPNLKVGICGEHGGEPSSIEFCHITGLDYVSCSPYRVPVARLAAAQAAIKHG
K

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=457, Percent_Identity=25.601750547046, Blast_Score=114, Evalue=4e-26,
Organism=Escherichia coli, GI48994992, Length=442, Percent_Identity=23.0769230769231, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1788726, Length=461, Percent_Identity=23.644251626898, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1789193, Length=465, Percent_Identity=23.010752688172, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 98082; Mature: 97951

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKYVYFFGKGRAEGDASMKQLLGGKGANLAEMVRLGVPVPPGFTISTEVCKYYYDHGK
CCCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
TYPEELKDQVLKAMKELEEATGKVFGSPENPLLVSVRSGAAVSMPGMMDTILNLGLNDET
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEECCHHHHHHHHCCCCHHH
VEGMIKSTNNPRFCYDAYRRFMQMFGDVVLNIKHEYFEEELNKVKTEKGVKLDTDLDADD
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHH
LKKVVERYKEVYKKAGHKFPQDPWDQLWKAIDAVFGSWMNERAIKYRELNGIKEGSLLGT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AVNVVAMVFGNMGEDSGTGVAFTRDPNTGEKKYYGEYLPNAQGEDVVAGIRTPYPLTKLE
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
EMMPDVYKQLLDIFEKLEKHYRDMQDIEFTIERGKLYLLQTRTGKRTPFAAVRIAVDMAE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCCCCHHHHHEEHHHHH
EGLITKEEAIMRVSPDHIETLLHPYFEPETKKNASLLAKGIAASPGAATGIIVFDPAEAE
CCCCCHHHHHEECCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCHHHH
RMVKEENKQVILVRPETSPEDIAGMAAAEGILTSTGGKTSHAAVVARGMGKPCIVGCEAI
HHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCCEEEECEEE
EVDEKAGVIRIDGKEIKEFSWLSIDGTTGEVFDGKLATVKPQGLEGPLAKILQWADEIRK
EECCCCCEEEECCHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
LGVRTNADTPRDATVARNFGAEGIGLCRTEHMFFEEERIPVVRQMIVAKTKEQREIALEK
CCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLPMQQEDFVGLFKAMEGYPVTIRLLDPPLHEFLPKEDEDIKALAEEMGLSYEELKATVE
HCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
ELHEFNPMMGHRGCRLSITYPEIAVMQTKAIIGAAIQVLKEEGKTVIPEIMIPLVGHINE
HHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHH
LKYLKKIITETADKMIEEAGVDLQYKVGTMIEIPRAALTADEIATEAEFFSFGTNDLTQM
HHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHH
TFGFSRDDYGKFVKEYIEKGILEVDPFQKLDRTGVGQLVKMGTEKGRSTRPNLKVGICGE
HCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCC
HGGEPSSIEFCHITGLDYVSCSPYRVPVARLAAAQAAIKHGK
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKKYVYFFGKGRAEGDASMKQLLGGKGANLAEMVRLGVPVPPGFTISTEVCKYYYDHGK
CCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
TYPEELKDQVLKAMKELEEATGKVFGSPENPLLVSVRSGAAVSMPGMMDTILNLGLNDET
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEECCHHHHHHHHCCCCHHH
VEGMIKSTNNPRFCYDAYRRFMQMFGDVVLNIKHEYFEEELNKVKTEKGVKLDTDLDADD
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHH
LKKVVERYKEVYKKAGHKFPQDPWDQLWKAIDAVFGSWMNERAIKYRELNGIKEGSLLGT
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AVNVVAMVFGNMGEDSGTGVAFTRDPNTGEKKYYGEYLPNAQGEDVVAGIRTPYPLTKLE
HHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCEEECCCCCCCHHHHH
EMMPDVYKQLLDIFEKLEKHYRDMQDIEFTIERGKLYLLQTRTGKRTPFAAVRIAVDMAE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCCCCHHHHHEEHHHHH
EGLITKEEAIMRVSPDHIETLLHPYFEPETKKNASLLAKGIAASPGAATGIIVFDPAEAE
CCCCCHHHHHEECCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCHHHH
RMVKEENKQVILVRPETSPEDIAGMAAAEGILTSTGGKTSHAAVVARGMGKPCIVGCEAI
HHHHHCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCCEEEECEEE
EVDEKAGVIRIDGKEIKEFSWLSIDGTTGEVFDGKLATVKPQGLEGPLAKILQWADEIRK
EECCCCCEEEECCHHHCCEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
LGVRTNADTPRDATVARNFGAEGIGLCRTEHMFFEEERIPVVRQMIVAKTKEQREIALEK
CCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LLPMQQEDFVGLFKAMEGYPVTIRLLDPPLHEFLPKEDEDIKALAEEMGLSYEELKATVE
HCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHH
ELHEFNPMMGHRGCRLSITYPEIAVMQTKAIIGAAIQVLKEEGKTVIPEIMIPLVGHINE
HHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHH
LKYLKKIITETADKMIEEAGVDLQYKVGTMIEIPRAALTADEIATEAEFFSFGTNDLTQM
HHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCHHHHHHHHHHHHHHHHHCCCCHHHHH
TFGFSRDDYGKFVKEYIEKGILEVDPFQKLDRTGVGQLVKMGTEKGRSTRPNLKVGICGE
HCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCC
HGGEPSSIEFCHITGLDYVSCSPYRVPVARLAAAQAAIKHGK
CCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]