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The map label for this gene is nudF [H]

Identifier: 239618083

GI number: 239618083

Start: 1845088

End: 1845615

Strand: Reverse

Name: nudF [H]

Synonym: Kole_1715

Alternate gene names: 239618083

Gene position: 1845615-1845088 (Counterclockwise)

Preceding gene: 239618084

Following gene: 239618082

Centisome position: 80.17

GC content: 42.05

Gene sequence:

>528_bases
ATGGAAAAATCTCTGGGTTTTAAAACATATTTCGAAGGAAAAGTGGTAAAACTCGAAAAACACGAGGTAGAGTTAGAGAA
TGGTTCAAAAAGCTTCCGCGAAGTGGTAAGACACCCGGGTGCCGTTGCTGTTGTAGCCCTTTTAGAAGACAGGGTATTGA
TGGTTAAACAGTACAGATTTCCCGTTGAAGATGATCTGCTGGAAATTCCGGCTGGAAAACTCGAAATGGGTGAGGATCCC
CTAGAATGCGCTAAAAGAGAATTAATGGAAGAAACAGGTTGCTCACCAAAAAAAATAACGCTTATGACTCAGTTATACAC
CTCCCCGGGGTTCTCTGATGAAAGGATATATCTTTATCTGGCGGAAGTAGAGCGTAACTCCGCACCCAATCCGGATGAAG
ACGAGATAATCGATGCCATCGAAATGCCTTTTGAAGAAGTTCTTCAGCTCATTTTAGAAGGAAAGATAAGGGATGGTAAA
ACCATTGCCGGAATTCTCTTTGCTGATAGAATTAGAAATAGGTGGTGA

Upstream 100 bases:

>100_bases
ATTTCGATGTAATTCTTGTGGGAAAAGAGAAAATCATCAGAACATCTGTTTCAGAATTGCTACCTTTACAATTCAAACTG
AATGAGGATGAGTGATATCT

Downstream 100 bases:

>100_bases
TATACAATGATCAGAGTAAGATTCGCGCCAAGTCCCACAGGATATTTGCACGTGGGTGGTGCAAGAACGGCGTTATTCAA
TTATCTCTTTGCAAGGCATA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MEKSLGFKTYFEGKVVKLEKHEVELENGSKSFREVVRHPGAVAVVALLEDRVLMVKQYRFPVEDDLLEIPAGKLEMGEDP
LECAKRELMEETGCSPKKITLMTQLYTSPGFSDERIYLYLAEVERNSAPNPDEDEIIDAIEMPFEEVLQLILEGKIRDGK
TIAGILFADRIRNRW

Sequences:

>Translated_175_residues
MEKSLGFKTYFEGKVVKLEKHEVELENGSKSFREVVRHPGAVAVVALLEDRVLMVKQYRFPVEDDLLEIPAGKLEMGEDP
LECAKRELMEETGCSPKKITLMTQLYTSPGFSDERIYLYLAEVERNSAPNPDEDEIIDAIEMPFEEVLQLILEGKIRDGK
TIAGILFADRIRNRW
>Mature_175_residues
MEKSLGFKTYFEGKVVKLEKHEVELENGSKSFREVVRHPGAVAVVALLEDRVLMVKQYRFPVEDDLLEIPAGKLEMGEDP
LECAKRELMEETGCSPKKITLMTQLYTSPGFSDERIYLYLAEVERNSAPNPDEDEIIDAIEMPFEEVLQLILEGKIRDGK
TIAGILFADRIRNRW

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=147, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20034; Mature: 20034

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSLGFKTYFEGKVVKLEKHEVELENGSKSFREVVRHPGAVAVVALLEDRVLMVKQYRF
CCCCCCCEEEECCEEEEEECCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PVEDDLLEIPAGKLEMGEDPLECAKRELMEETGCSPKKITLMTQLYTSPGFSDERIYLYL
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCEEEEEE
AEVERNSAPNPDEDEIIDAIEMPFEEVLQLILEGKIRDGKTIAGILFADRIRNRW
EEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKSLGFKTYFEGKVVKLEKHEVELENGSKSFREVVRHPGAVAVVALLEDRVLMVKQYRF
CCCCCCCEEEECCEEEEEECCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
PVEDDLLEIPAGKLEMGEDPLECAKRELMEETGCSPKKITLMTQLYTSPGFSDERIYLYL
CCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCEEEEEE
AEVERNSAPNPDEDEIIDAIEMPFEEVLQLILEGKIRDGKTIAGILFADRIRNRW
EEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]