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The map label for this gene is pfp [H]

Identifier: 239618066

GI number: 239618066

Start: 1825074

End: 1826096

Strand: Reverse

Name: pfp [H]

Synonym: Kole_1696

Alternate gene names: 239618066

Gene position: 1826096-1825074 (Counterclockwise)

Preceding gene: 239618070

Following gene: 239618065

Centisome position: 79.32

GC content: 44.77

Gene sequence:

>1023_bases
ATGAAGGTCGGAATTCTTACAGGTGGTGGAGACTGTCCGGGGCTAAACGCGGTTATTCGTGGAATCGTTAAATCAGCTCC
CAAAGACATAGAAATTATCGGGATAATGCACGGTTGGAAAGGGCTGATTACTGGCGAGGTCACAAAACTTACTGACGATG
ACGTCGAAGGGATTCACATCATAGGTGGAACGATACTCGGTACCTCAAGAACAAACCCTTTTAAAAGCGAGGAAAACAAG
AATAAAATGCTTGAAAACATAGAGAAACTCGGGCTTGATGCCCTCATCGCTATTGGTGGAGACGATACTCTGTCTGTCGC
AGCAAAGTTATCCTCTCTGGGTTACAAGGTTATAGGTGTTCCAAAAACCATAGATAACGACGTTTCCAACACCGATTACA
CATTTGGCTATCATACAGCGGTGAACGTTGGTGCTGATGCCATAGACAGACTTCATTCAACAGCAAAATCCCACGGGAGA
GTTCTCTTCGTTGAGCTCATGGGCAGAGAAGCAGGCTGGATAACATTAGAAGCAGGACTTGCAGCTGGTGCTCATTTGAT
ACTAATCCCCGAATACCCGATGACTCTTGAAGAGATCGTCAGGTACGTGAATGAAAGGATGGATAAAAAGAGCTACATGA
TCATAGCGGTTGCGGAAGGATTTAAACCCACTGACCTTGAAAATGTCATCGGTGATAAGAATGAAGTCGATGCCTTTGGC
CATATACGTCTTGGAGGAATCGCCCATTACCTTGCTGAAATAATAAAGGAGAAAACCGGATATGATACCCGCTCTGTTGT
TCTTGGACATCTGTTAAGGGGCGGAACACCCACGGCTTTTGACAGGATACTTGGAACCCGTTACGGTGTTCATGCAATGA
AACTCGTTCTCGAAAAGGATTTTGGTAGAATGGTAGCTCTGCGTGGAAATGCAATCGTTTCAATTCCTCTGGAATATGGG
ATAGCCACCAAAAAATTCGTTCCCTTCGAATACTACAAACTCGCGCAGATATTCTTTGGATGA

Upstream 100 bases:

>100_bases
CAAGCGTTCCTCCTCTCGGAGAGAGATAGATGTTCATGGTATAATTATACACGAAATGTTCTTTTTGAATCTCACTTCTA
GAGCGCAGGAGGTAATGAAA

Downstream 100 bases:

>100_bases
AGGGGTGAGTACCGCGAGATCGATATATGTTGAATGGGCCTGGCATGTAATCGAATCTTACATACTCAGGAATGAATATC
CAGGCATTTCTGGGAATTTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKVGILTGGGDCPGLNAVIRGIVKSAPKDIEIIGIMHGWKGLITGEVTKLTDDDVEGIHIIGGTILGTSRTNPFKSEENK
NKMLENIEKLGLDALIAIGGDDTLSVAAKLSSLGYKVIGVPKTIDNDVSNTDYTFGYHTAVNVGADAIDRLHSTAKSHGR
VLFVELMGREAGWITLEAGLAAGAHLILIPEYPMTLEEIVRYVNERMDKKSYMIIAVAEGFKPTDLENVIGDKNEVDAFG
HIRLGGIAHYLAEIIKEKTGYDTRSVVLGHLLRGGTPTAFDRILGTRYGVHAMKLVLEKDFGRMVALRGNAIVSIPLEYG
IATKKFVPFEYYKLAQIFFG

Sequences:

>Translated_340_residues
MKVGILTGGGDCPGLNAVIRGIVKSAPKDIEIIGIMHGWKGLITGEVTKLTDDDVEGIHIIGGTILGTSRTNPFKSEENK
NKMLENIEKLGLDALIAIGGDDTLSVAAKLSSLGYKVIGVPKTIDNDVSNTDYTFGYHTAVNVGADAIDRLHSTAKSHGR
VLFVELMGREAGWITLEAGLAAGAHLILIPEYPMTLEEIVRYVNERMDKKSYMIIAVAEGFKPTDLENVIGDKNEVDAFG
HIRLGGIAHYLAEIIKEKTGYDTRSVVLGHLLRGGTPTAFDRILGTRYGVHAMKLVLEKDFGRMVALRGNAIVSIPLEYG
IATKKFVPFEYYKLAQIFFG
>Mature_340_residues
MKVGILTGGGDCPGLNAVIRGIVKSAPKDIEIIGIMHGWKGLITGEVTKLTDDDVEGIHIIGGTILGTSRTNPFKSEENK
NKMLENIEKLGLDALIAIGGDDTLSVAAKLSSLGYKVIGVPKTIDNDVSNTDYTFGYHTAVNVGADAIDRLHSTAKSHGR
VLFVELMGREAGWITLEAGLAAGAHLILIPEYPMTLEEIVRYVNERMDKKSYMIIAVAEGFKPTDLENVIGDKNEVDAFG
HIRLGGIAHYLAEIIKEKTGYDTRSVVLGHLLRGGTPTAFDRILGTRYGVHAMKLVLEKDFGRMVALRGNAIVSIPLEYG
IATKKFVPFEYYKLAQIFFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=348, Percent_Identity=32.183908045977, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI266453768, Length=363, Percent_Identity=31.6804407713499, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI266453748, Length=363, Percent_Identity=31.6804407713499, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4505749, Length=363, Percent_Identity=31.6804407713499, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI266453619, Length=363, Percent_Identity=31.6804407713499, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI48762920, Length=364, Percent_Identity=29.9450549450549, Blast_Score=148, Evalue=6e-36,
Organism=Escherichia coli, GI1790350, Length=314, Percent_Identity=38.2165605095541, Blast_Score=217, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI25147584, Length=348, Percent_Identity=31.0344827586207, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17558788, Length=352, Percent_Identity=29.5454545454545, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI72003601, Length=309, Percent_Identity=27.1844660194175, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323861, Length=360, Percent_Identity=33.0555555555556, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321679, Length=362, Percent_Identity=32.0441988950276, Blast_Score=161, Evalue=1e-40,
Organism=Drosophila melanogaster, GI28573326, Length=348, Percent_Identity=31.8965517241379, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24652337, Length=348, Percent_Identity=32.4712643678161, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI17647809, Length=348, Percent_Identity=32.4712643678161, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 36900; Mature: 36900

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGILTGGGDCPGLNAVIRGIVKSAPKDIEIIGIMHGWKGLITGEVTKLTDDDVEGIHI
CEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCEEECCCCCCCEEEE
IGGTILGTSRTNPFKSEENKNKMLENIEKLGLDALIAIGGDDTLSVAAKLSSLGYKVIGV
EEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEC
PKTIDNDVSNTDYTFGYHTAVNVGADAIDRLHSTAKSHGRVLFVELMGREAGWITLEAGL
CCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
AAGAHLILIPEYPMTLEEIVRYVNERMDKKSYMIIAVAEGFKPTDLENVIGDKNEVDAFG
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCC
HIRLGGIAHYLAEIIKEKTGYDTRSVVLGHLLRGGTPTAFDRILGTRYGVHAMKLVLEKD
CEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHC
FGRMVALRGNAIVSIPLEYGIATKKFVPFEYYKLAQIFFG
CCCEEEEECCEEEEEEHHCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKVGILTGGGDCPGLNAVIRGIVKSAPKDIEIIGIMHGWKGLITGEVTKLTDDDVEGIHI
CEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEECCCEEECCCCCCCEEEE
IGGTILGTSRTNPFKSEENKNKMLENIEKLGLDALIAIGGDDTLSVAAKLSSLGYKVIGV
EEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEC
PKTIDNDVSNTDYTFGYHTAVNVGADAIDRLHSTAKSHGRVLFVELMGREAGWITLEAGL
CCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEECCC
AAGAHLILIPEYPMTLEEIVRYVNERMDKKSYMIIAVAEGFKPTDLENVIGDKNEVDAFG
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCCCCCCCCC
HIRLGGIAHYLAEIIKEKTGYDTRSVVLGHLLRGGTPTAFDRILGTRYGVHAMKLVLEKD
CEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHC
FGRMVALRGNAIVSIPLEYGIATKKFVPFEYYKLAQIFFG
CCCEEEEECCEEEEEEHHCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550409 [H]